Amino acid dipepetide frequency for Streptomyces phage Wentworth

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.469AlaAla: 12.469 ± 1.713
1.134AlaCys: 1.134 ± 0.23
7.639AlaAsp: 7.639 ± 0.478
7.737AlaGlu: 7.737 ± 0.86
2.563AlaPhe: 2.563 ± 0.375
8.082AlaGly: 8.082 ± 0.805
1.577AlaHis: 1.577 ± 0.326
4.435AlaIle: 4.435 ± 0.461
5.717AlaLys: 5.717 ± 0.787
7.737AlaLeu: 7.737 ± 0.546
3.006AlaMet: 3.006 ± 0.466
3.943AlaAsn: 3.943 ± 0.565
3.105AlaPro: 3.105 ± 0.384
4.14AlaGln: 4.14 ± 0.628
5.224AlaArg: 5.224 ± 0.507
4.534AlaSer: 4.534 ± 0.513
7.097AlaThr: 7.097 ± 0.958
5.963AlaVal: 5.963 ± 0.539
1.429AlaTrp: 1.429 ± 0.313
2.267AlaTyr: 2.267 ± 0.402
0.0AlaXaa: 0.0 ± 0.0
Cys
0.838CysAla: 0.838 ± 0.193
0.246CysCys: 0.246 ± 0.114
0.789CysAsp: 0.789 ± 0.206
1.134CysGlu: 1.134 ± 0.307
0.296CysPhe: 0.296 ± 0.136
0.838CysGly: 0.838 ± 0.26
0.148CysHis: 0.148 ± 0.074
0.345CysIle: 0.345 ± 0.129
0.838CysLys: 0.838 ± 0.297
0.641CysLeu: 0.641 ± 0.236
0.296CysMet: 0.296 ± 0.139
0.69CysAsn: 0.69 ± 0.205
0.641CysPro: 0.641 ± 0.245
0.69CysGln: 0.69 ± 0.176
0.838CysArg: 0.838 ± 0.218
0.838CysSer: 0.838 ± 0.234
0.739CysThr: 0.739 ± 0.262
0.394CysVal: 0.394 ± 0.134
0.246CysTrp: 0.246 ± 0.103
0.296CysTyr: 0.296 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
7.737AspAla: 7.737 ± 0.749
0.838AspCys: 0.838 ± 0.231
6.752AspAsp: 6.752 ± 0.918
6.752AspGlu: 6.752 ± 0.875
2.661AspPhe: 2.661 ± 0.302
6.505AspGly: 6.505 ± 0.554
1.577AspHis: 1.577 ± 0.357
2.957AspIle: 2.957 ± 0.374
2.661AspLys: 2.661 ± 0.449
6.505AspLeu: 6.505 ± 0.567
1.774AspMet: 1.774 ± 0.345
2.366AspAsn: 2.366 ± 0.304
3.992AspPro: 3.992 ± 0.634
2.563AspGln: 2.563 ± 0.541
3.499AspArg: 3.499 ± 0.463
3.598AspSer: 3.598 ± 0.387
3.746AspThr: 3.746 ± 0.709
3.992AspVal: 3.992 ± 0.415
1.134AspTrp: 1.134 ± 0.257
2.021AspTyr: 2.021 ± 0.275
0.0AspXaa: 0.0 ± 0.0
Glu
8.082GluAla: 8.082 ± 0.879
1.035GluCys: 1.035 ± 0.247
6.111GluAsp: 6.111 ± 0.957
5.717GluGlu: 5.717 ± 0.944
2.661GluPhe: 2.661 ± 0.32
4.386GluGly: 4.386 ± 0.51
1.774GluHis: 1.774 ± 0.368
3.203GluIle: 3.203 ± 0.419
2.661GluLys: 2.661 ± 0.467
6.111GluLeu: 6.111 ± 0.681
2.119GluMet: 2.119 ± 0.296
2.563GluAsn: 2.563 ± 0.376
3.154GluPro: 3.154 ± 0.423
3.401GluGln: 3.401 ± 0.474
4.337GluArg: 4.337 ± 0.572
3.006GluSer: 3.006 ± 0.315
3.056GluThr: 3.056 ± 0.456
4.337GluVal: 4.337 ± 0.443
0.936GluTrp: 0.936 ± 0.193
1.577GluTyr: 1.577 ± 0.374
0.0GluXaa: 0.0 ± 0.0
Phe
2.858PheAla: 2.858 ± 0.374
0.246PheCys: 0.246 ± 0.109
2.267PheAsp: 2.267 ± 0.311
2.415PheGlu: 2.415 ± 0.374
0.838PhePhe: 0.838 ± 0.213
2.908PheGly: 2.908 ± 0.348
0.296PheHis: 0.296 ± 0.137
1.281PheIle: 1.281 ± 0.273
1.232PheLys: 1.232 ± 0.236
2.168PheLeu: 2.168 ± 0.32
0.739PheMet: 0.739 ± 0.184
1.134PheAsn: 1.134 ± 0.266
1.232PhePro: 1.232 ± 0.334
1.084PheGln: 1.084 ± 0.243
2.07PheArg: 2.07 ± 0.338
1.478PheSer: 1.478 ± 0.532
1.232PheThr: 1.232 ± 0.199
2.021PheVal: 2.021 ± 0.264
0.739PheTrp: 0.739 ± 0.269
0.69PheTyr: 0.69 ± 0.194
0.0PheXaa: 0.0 ± 0.0
Gly
6.407GlyAla: 6.407 ± 0.649
0.986GlyCys: 0.986 ± 0.249
6.357GlyAsp: 6.357 ± 1.126
5.668GlyGlu: 5.668 ± 0.5
2.415GlyPhe: 2.415 ± 0.373
6.259GlyGly: 6.259 ± 0.603
1.38GlyHis: 1.38 ± 0.285
3.795GlyIle: 3.795 ± 0.453
4.435GlyLys: 4.435 ± 0.511
5.273GlyLeu: 5.273 ± 0.548
2.76GlyMet: 2.76 ± 0.446
3.006GlyAsn: 3.006 ± 0.353
4.189GlyPro: 4.189 ± 0.399
2.218GlyGln: 2.218 ± 0.292
4.83GlyArg: 4.83 ± 0.603
4.928GlySer: 4.928 ± 0.557
4.928GlyThr: 4.928 ± 0.628
6.357GlyVal: 6.357 ± 0.605
2.119GlyTrp: 2.119 ± 0.461
2.612GlyTyr: 2.612 ± 0.371
0.0GlyXaa: 0.0 ± 0.0
His
1.676HisAla: 1.676 ± 0.259
0.246HisCys: 0.246 ± 0.108
1.035HisAsp: 1.035 ± 0.262
1.676HisGlu: 1.676 ± 0.353
0.493HisPhe: 0.493 ± 0.175
1.281HisGly: 1.281 ± 0.244
0.542HisHis: 0.542 ± 0.189
0.986HisIle: 0.986 ± 0.185
1.528HisLys: 1.528 ± 0.311
1.873HisLeu: 1.873 ± 0.262
0.641HisMet: 0.641 ± 0.192
0.493HisAsn: 0.493 ± 0.134
0.986HisPro: 0.986 ± 0.23
0.591HisGln: 0.591 ± 0.196
1.281HisArg: 1.281 ± 0.274
0.739HisSer: 0.739 ± 0.188
1.232HisThr: 1.232 ± 0.237
1.331HisVal: 1.331 ± 0.26
0.591HisTrp: 0.591 ± 0.166
0.542HisTyr: 0.542 ± 0.151
0.0HisXaa: 0.0 ± 0.0
Ile
4.041IleAla: 4.041 ± 0.405
0.493IleCys: 0.493 ± 0.155
3.746IleAsp: 3.746 ± 0.49
2.76IleGlu: 2.76 ± 0.461
0.936IlePhe: 0.936 ± 0.195
3.056IleGly: 3.056 ± 0.468
0.986IleHis: 0.986 ± 0.263
2.021IleIle: 2.021 ± 0.366
2.563IleLys: 2.563 ± 0.365
3.203IleLeu: 3.203 ± 0.417
0.838IleMet: 0.838 ± 0.213
1.528IleAsn: 1.528 ± 0.238
2.809IlePro: 2.809 ± 0.377
2.415IleGln: 2.415 ± 0.278
3.056IleArg: 3.056 ± 0.435
2.415IleSer: 2.415 ± 0.29
3.006IleThr: 3.006 ± 0.516
3.203IleVal: 3.203 ± 0.379
0.641IleTrp: 0.641 ± 0.182
0.936IleTyr: 0.936 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
5.027LysAla: 5.027 ± 0.637
0.444LysCys: 0.444 ± 0.158
3.154LysAsp: 3.154 ± 0.411
3.006LysGlu: 3.006 ± 0.504
1.035LysPhe: 1.035 ± 0.226
4.09LysGly: 4.09 ± 0.573
1.035LysHis: 1.035 ± 0.253
2.267LysIle: 2.267 ± 0.304
2.908LysLys: 2.908 ± 0.489
4.78LysLeu: 4.78 ± 0.575
1.922LysMet: 1.922 ± 0.398
1.528LysAsn: 1.528 ± 0.319
3.302LysPro: 3.302 ± 0.447
2.119LysGln: 2.119 ± 0.385
3.154LysArg: 3.154 ± 0.493
3.203LysSer: 3.203 ± 0.379
3.45LysThr: 3.45 ± 0.485
3.203LysVal: 3.203 ± 0.468
1.478LysTrp: 1.478 ± 0.277
1.134LysTyr: 1.134 ± 0.232
0.0LysXaa: 0.0 ± 0.0
Leu
7.343LeuAla: 7.343 ± 0.579
1.035LeuCys: 1.035 ± 0.238
4.978LeuAsp: 4.978 ± 0.537
4.978LeuGlu: 4.978 ± 0.546
2.119LeuPhe: 2.119 ± 0.316
6.259LeuGly: 6.259 ± 0.51
1.676LeuHis: 1.676 ± 0.292
3.351LeuIle: 3.351 ± 0.448
4.09LeuLys: 4.09 ± 0.599
4.583LeuLeu: 4.583 ± 0.454
2.119LeuMet: 2.119 ± 0.347
3.105LeuAsn: 3.105 ± 0.445
4.041LeuPro: 4.041 ± 0.5
2.464LeuGln: 2.464 ± 0.349
5.52LeuArg: 5.52 ± 0.722
5.421LeuSer: 5.421 ± 0.541
4.682LeuThr: 4.682 ± 0.662
5.372LeuVal: 5.372 ± 0.646
1.183LeuTrp: 1.183 ± 0.241
2.563LeuTyr: 2.563 ± 0.353
0.0LeuXaa: 0.0 ± 0.0
Met
3.253MetAla: 3.253 ± 0.519
0.296MetCys: 0.296 ± 0.126
2.366MetAsp: 2.366 ± 0.291
1.823MetGlu: 1.823 ± 0.371
0.542MetPhe: 0.542 ± 0.143
1.971MetGly: 1.971 ± 0.378
0.394MetHis: 0.394 ± 0.148
1.281MetIle: 1.281 ± 0.221
1.478MetLys: 1.478 ± 0.298
2.168MetLeu: 2.168 ± 0.318
0.69MetMet: 0.69 ± 0.178
0.986MetAsn: 0.986 ± 0.213
1.725MetPro: 1.725 ± 0.342
1.183MetGln: 1.183 ± 0.199
2.119MetArg: 2.119 ± 0.302
2.218MetSer: 2.218 ± 0.345
1.725MetThr: 1.725 ± 0.361
1.232MetVal: 1.232 ± 0.238
0.739MetTrp: 0.739 ± 0.175
0.789MetTyr: 0.789 ± 0.213
0.0MetXaa: 0.0 ± 0.0
Asn
3.696AsnAla: 3.696 ± 0.495
0.493AsnCys: 0.493 ± 0.144
2.711AsnAsp: 2.711 ± 0.373
2.513AsnGlu: 2.513 ± 0.387
0.493AsnPhe: 0.493 ± 0.153
3.45AsnGly: 3.45 ± 0.477
0.542AsnHis: 0.542 ± 0.164
1.134AsnIle: 1.134 ± 0.311
2.218AsnLys: 2.218 ± 0.316
1.725AsnLeu: 1.725 ± 0.282
0.887AsnMet: 0.887 ± 0.197
1.084AsnAsn: 1.084 ± 0.236
2.07AsnPro: 2.07 ± 0.314
2.119AsnGln: 2.119 ± 0.361
1.873AsnArg: 1.873 ± 0.312
1.676AsnSer: 1.676 ± 0.219
2.563AsnThr: 2.563 ± 0.337
2.513AsnVal: 2.513 ± 0.501
0.641AsnTrp: 0.641 ± 0.223
0.789AsnTyr: 0.789 ± 0.152
0.0AsnXaa: 0.0 ± 0.0
Pro
4.485ProAla: 4.485 ± 0.449
0.345ProCys: 0.345 ± 0.134
3.548ProAsp: 3.548 ± 0.465
4.337ProGlu: 4.337 ± 0.515
0.986ProPhe: 0.986 ± 0.188
3.795ProGly: 3.795 ± 0.376
1.035ProHis: 1.035 ± 0.218
1.528ProIle: 1.528 ± 0.251
2.858ProLys: 2.858 ± 0.389
3.203ProLeu: 3.203 ± 0.443
1.084ProMet: 1.084 ± 0.208
1.134ProAsn: 1.134 ± 0.225
1.626ProPro: 1.626 ± 0.359
1.478ProGln: 1.478 ± 0.297
2.119ProArg: 2.119 ± 0.47
3.253ProSer: 3.253 ± 0.381
3.499ProThr: 3.499 ± 0.657
3.844ProVal: 3.844 ± 0.524
0.789ProTrp: 0.789 ± 0.204
1.676ProTyr: 1.676 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
4.78GlnAla: 4.78 ± 0.556
0.345GlnCys: 0.345 ± 0.156
2.021GlnAsp: 2.021 ± 0.277
1.528GlnGlu: 1.528 ± 0.284
1.232GlnPhe: 1.232 ± 0.327
2.957GlnGly: 2.957 ± 0.423
0.591GlnHis: 0.591 ± 0.169
1.676GlnIle: 1.676 ± 0.271
2.267GlnLys: 2.267 ± 0.365
3.401GlnLeu: 3.401 ± 0.34
1.429GlnMet: 1.429 ± 0.259
0.986GlnAsn: 0.986 ± 0.188
1.429GlnPro: 1.429 ± 0.296
1.429GlnGln: 1.429 ± 0.26
2.858GlnArg: 2.858 ± 0.354
2.267GlnSer: 2.267 ± 0.406
2.168GlnThr: 2.168 ± 0.428
1.823GlnVal: 1.823 ± 0.289
0.838GlnTrp: 0.838 ± 0.201
1.528GlnTyr: 1.528 ± 0.431
0.0GlnXaa: 0.0 ± 0.0
Arg
4.928ArgAla: 4.928 ± 0.49
0.69ArgCys: 0.69 ± 0.188
3.795ArgAsp: 3.795 ± 0.48
4.485ArgGlu: 4.485 ± 0.702
2.218ArgPhe: 2.218 ± 0.357
4.386ArgGly: 4.386 ± 0.466
1.626ArgHis: 1.626 ± 0.366
3.647ArgIle: 3.647 ± 0.546
3.943ArgLys: 3.943 ± 0.449
5.421ArgLeu: 5.421 ± 0.755
2.267ArgMet: 2.267 ± 0.355
1.823ArgAsn: 1.823 ± 0.351
2.218ArgPro: 2.218 ± 0.31
1.774ArgGln: 1.774 ± 0.347
4.83ArgArg: 4.83 ± 0.759
3.302ArgSer: 3.302 ± 0.443
2.661ArgThr: 2.661 ± 0.376
3.943ArgVal: 3.943 ± 0.414
1.38ArgTrp: 1.38 ± 0.256
2.218ArgTyr: 2.218 ± 0.429
0.0ArgXaa: 0.0 ± 0.0
Ser
5.52SerAla: 5.52 ± 0.56
0.789SerCys: 0.789 ± 0.174
3.746SerAsp: 3.746 ± 0.525
3.105SerGlu: 3.105 ± 0.389
1.429SerPhe: 1.429 ± 0.336
6.308SerGly: 6.308 ± 0.811
0.986SerHis: 0.986 ± 0.206
2.218SerIle: 2.218 ± 0.472
2.563SerLys: 2.563 ± 0.409
4.534SerLeu: 4.534 ± 0.479
1.873SerMet: 1.873 ± 0.303
2.661SerAsn: 2.661 ± 0.344
2.366SerPro: 2.366 ± 0.34
1.676SerGln: 1.676 ± 0.327
2.563SerArg: 2.563 ± 0.398
3.598SerSer: 3.598 ± 0.59
3.056SerThr: 3.056 ± 0.422
4.14SerVal: 4.14 ± 0.593
1.134SerTrp: 1.134 ± 0.216
1.873SerTyr: 1.873 ± 0.303
0.0SerXaa: 0.0 ± 0.0
Thr
5.52ThrAla: 5.52 ± 0.714
0.591ThrCys: 0.591 ± 0.204
3.253ThrAsp: 3.253 ± 0.594
3.401ThrGlu: 3.401 ± 0.454
2.563ThrPhe: 2.563 ± 0.515
6.062ThrGly: 6.062 ± 1.101
1.232ThrHis: 1.232 ± 0.261
3.45ThrIle: 3.45 ± 0.457
2.021ThrLys: 2.021 ± 0.321
4.83ThrLeu: 4.83 ± 0.701
1.676ThrMet: 1.676 ± 0.467
1.922ThrAsn: 1.922 ± 0.353
3.203ThrPro: 3.203 ± 0.448
1.429ThrGln: 1.429 ± 0.271
4.238ThrArg: 4.238 ± 0.44
3.105ThrSer: 3.105 ± 0.652
3.943ThrThr: 3.943 ± 0.7
4.83ThrVal: 4.83 ± 0.671
1.134ThrTrp: 1.134 ± 0.228
1.331ThrTyr: 1.331 ± 0.257
0.0ThrXaa: 0.0 ± 0.0
Val
6.702ValAla: 6.702 ± 0.675
0.641ValCys: 0.641 ± 0.202
5.618ValAsp: 5.618 ± 0.887
4.288ValGlu: 4.288 ± 0.529
2.021ValPhe: 2.021 ± 0.298
5.027ValGly: 5.027 ± 0.636
1.478ValHis: 1.478 ± 0.268
3.105ValIle: 3.105 ± 0.357
3.893ValLys: 3.893 ± 0.571
5.273ValLeu: 5.273 ± 0.546
1.626ValMet: 1.626 ± 0.388
2.76ValAsn: 2.76 ± 0.374
2.76ValPro: 2.76 ± 0.403
2.809ValGln: 2.809 ± 0.329
4.041ValArg: 4.041 ± 0.361
3.992ValSer: 3.992 ± 0.449
3.992ValThr: 3.992 ± 0.609
4.879ValVal: 4.879 ± 0.478
1.281ValTrp: 1.281 ± 0.291
1.823ValTyr: 1.823 ± 0.31
0.0ValXaa: 0.0 ± 0.0
Trp
1.577TrpAla: 1.577 ± 0.291
0.394TrpCys: 0.394 ± 0.154
1.676TrpAsp: 1.676 ± 0.337
1.232TrpGlu: 1.232 ± 0.274
0.444TrpPhe: 0.444 ± 0.136
0.789TrpGly: 0.789 ± 0.175
0.394TrpHis: 0.394 ± 0.145
0.986TrpIle: 0.986 ± 0.234
0.986TrpLys: 0.986 ± 0.261
1.922TrpLeu: 1.922 ± 0.304
0.641TrpMet: 0.641 ± 0.187
0.542TrpAsn: 0.542 ± 0.196
0.887TrpPro: 0.887 ± 0.187
0.69TrpGln: 0.69 ± 0.174
1.134TrpArg: 1.134 ± 0.264
1.183TrpSer: 1.183 ± 0.236
1.478TrpThr: 1.478 ± 0.418
1.281TrpVal: 1.281 ± 0.252
0.345TrpTrp: 0.345 ± 0.115
0.789TrpTyr: 0.789 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.908TyrAla: 2.908 ± 0.348
0.493TyrCys: 0.493 ± 0.192
2.07TyrAsp: 2.07 ± 0.371
1.676TyrGlu: 1.676 ± 0.301
0.986TyrPhe: 0.986 ± 0.253
2.513TyrGly: 2.513 ± 0.393
0.493TyrHis: 0.493 ± 0.148
0.986TyrIle: 0.986 ± 0.208
1.38TyrLys: 1.38 ± 0.343
1.528TyrLeu: 1.528 ± 0.288
0.542TyrMet: 0.542 ± 0.137
0.936TyrAsn: 0.936 ± 0.212
0.887TyrPro: 0.887 ± 0.234
1.281TyrGln: 1.281 ± 0.29
1.971TyrArg: 1.971 ± 0.323
1.38TyrSer: 1.38 ± 0.243
1.478TyrThr: 1.478 ± 0.269
3.351TyrVal: 3.351 ± 0.769
0.591TyrTrp: 0.591 ± 0.198
0.591TyrTyr: 0.591 ± 0.196
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 103 proteins (20292 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski