Rubinisphaera italica

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Rubinisphaera

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C5XML0|A0A5C5XML0_9PLAN Galactose-1-phosphate uridylyltransferase OS=Rubinisphaera italica OX=2527969 GN=galT PE=3 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84LSDD5 pKa = 3.77VLDD8 pKa = 3.63VAGEE12 pKa = 4.16FQWSDD17 pKa = 3.24ALYY20 pKa = 10.86LPINTAFTLNTPAIVYY36 pKa = 10.44DD37 pKa = 4.1PDD39 pKa = 4.51DD40 pKa = 3.92VVDD43 pKa = 5.45DD44 pKa = 4.38GQEE47 pKa = 4.16TPVFPSEE54 pKa = 3.69NGMVYY59 pKa = 10.55VLGINTVQEE68 pKa = 3.98ILSNLNQQKK77 pKa = 10.1PEE79 pKa = 4.01CTADD83 pKa = 3.93DD84 pKa = 3.8KK85 pKa = 11.79LKK87 pKa = 11.06ALNHH91 pKa = 5.78YY92 pKa = 8.77VLNDD96 pKa = 3.6AFICLNN102 pKa = 3.59

Molecular weight: 11.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C5XA96|A0A5C5XA96_9PLAN Uncharacterized protein OS=Rubinisphaera italica OX=2527969 GN=Pan54_00210 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 5.02SLIVLISGTATGIVAGLFGVGGSFLLVPLLSITTSIPMEE41 pKa = 4.79LIVGSCACQVLGPATAASLSFRR63 pKa = 11.84QRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84DD68 pKa = 3.37LRR70 pKa = 11.84IPFILMGGIIAGTLWGSASLNQLKK94 pKa = 9.68EE95 pKa = 4.3QIGSNADD102 pKa = 3.38SLSNIVQITYY112 pKa = 10.74LVLLWSLGLFSLWEE126 pKa = 4.21SMLHH130 pKa = 6.21KK131 pKa = 10.02RR132 pKa = 11.84GKK134 pKa = 10.11LIPIGWARR142 pKa = 11.84LKK144 pKa = 9.89WLRR147 pKa = 11.84PVCEE151 pKa = 3.74VFGRR155 pKa = 11.84NRR157 pKa = 11.84RR158 pKa = 11.84HH159 pKa = 4.58QVSIISLSWFGVFVGFLSGFIGLSGGVILLPGLHH193 pKa = 6.04YY194 pKa = 10.59AYY196 pKa = 10.39GIPTKK201 pKa = 10.24RR202 pKa = 11.84AARR205 pKa = 11.84MSMLLVWLIAIQATVIHH222 pKa = 6.12ATYY225 pKa = 10.53QRR227 pKa = 11.84VDD229 pKa = 3.46LQTVVVLLLGGTLGAKK245 pKa = 10.25LGVQLSEE252 pKa = 4.15RR253 pKa = 11.84MSGGALRR260 pKa = 11.84QHH262 pKa = 6.48FAWLLLITAAVLSIYY277 pKa = 10.83SLTKK281 pKa = 10.4

Molecular weight: 30.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5229

0

5229

1885334

29

9718

360.6

40.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.996 ± 0.041

1.217 ± 0.015

5.707 ± 0.031

6.874 ± 0.033

3.979 ± 0.019

7.005 ± 0.049

2.28 ± 0.019

5.94 ± 0.025

4.521 ± 0.038

9.88 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.017

3.964 ± 0.028

4.91 ± 0.03

4.544 ± 0.029

5.658 ± 0.037

6.822 ± 0.029

5.604 ± 0.044

6.523 ± 0.033

1.477 ± 0.017

2.775 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski