Aminomonas paucivorans DSM 12260
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2391 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E3CYW7|E3CYW7_9BACT Transcriptional regulator AraC family OS=Aminomonas paucivorans DSM 12260 OX=584708 GN=Apau_2151 PE=4 SV=1
MM1 pKa = 7.72 AARR4 pKa = 11.84 EE5 pKa = 4.02 KK6 pKa = 10.37 IAYY9 pKa = 9.46 LKK11 pKa = 11.04 GLIDD15 pKa = 3.91 GQKK18 pKa = 10.25 PADD21 pKa = 4.19 PDD23 pKa = 3.81 TAKK26 pKa = 11.15 LLGAVVDD33 pKa = 4.62 ALDD36 pKa = 4.15 GLADD40 pKa = 4.01 DD41 pKa = 5.84 LEE43 pKa = 4.65 NQGIRR48 pKa = 11.84 LEE50 pKa = 4.11 EE51 pKa = 3.86 HH52 pKa = 6.9 RR53 pKa = 11.84 EE54 pKa = 3.77 ILDD57 pKa = 3.65 EE58 pKa = 4.1 VSEE61 pKa = 4.18 YY62 pKa = 11.04 LDD64 pKa = 4.79 QIDD67 pKa = 4.58 EE68 pKa = 4.41 DD69 pKa = 4.26 LCALEE74 pKa = 5.93 DD75 pKa = 4.59 RR76 pKa = 11.84 IEE78 pKa = 4.2 SCEE81 pKa = 4.0 DD82 pKa = 3.22 EE83 pKa = 4.89 EE84 pKa = 4.37 EE85 pKa = 5.56 GEE87 pKa = 4.92 DD88 pKa = 4.27 EE89 pKa = 4.33 EE90 pKa = 4.58 EE91 pKa = 5.15 DD92 pKa = 4.25 YY93 pKa = 11.32 ISVCCPHH100 pKa = 6.45 CRR102 pKa = 11.84 KK103 pKa = 10.49 DD104 pKa = 3.6 FFYY107 pKa = 11.1 DD108 pKa = 3.37 PTAYY112 pKa = 10.08 EE113 pKa = 3.91 EE114 pKa = 5.91 DD115 pKa = 3.94 EE116 pKa = 5.6 DD117 pKa = 5.29 LLCPHH122 pKa = 7.02 CGEE125 pKa = 4.75 PFKK128 pKa = 11.05 QPEE131 pKa = 3.85 II132 pKa = 4.05
Molecular weight: 15.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.859
IPC_protein 3.846
Toseland 3.643
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.668
Grimsley 3.554
Solomon 3.808
Lehninger 3.757
Nozaki 3.923
DTASelect 4.101
Thurlkill 3.681
EMBOSS 3.719
Sillero 3.961
Patrickios 1.875
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.852
Protein with the highest isoelectric point:
>tr|E3D143|E3D143_9BACT Asparaginase/glutaminase OS=Aminomonas paucivorans DSM 12260 OX=584708 GN=Apau_1530 PE=4 SV=1
MM1 pKa = 7.82 IKK3 pKa = 10.17 PQGARR8 pKa = 11.84 AALLLPLLPLTLFLLSFTLGRR29 pKa = 11.84 YY30 pKa = 7.68 PIPLPTVLKK39 pKa = 10.42 ILAAKK44 pKa = 8.06 FFPLTPHH51 pKa = 6.62 WPDD54 pKa = 2.92 TMEE57 pKa = 4.06 TVLWNLRR64 pKa = 11.84 LPRR67 pKa = 11.84 ALGAMMVGAALSSSGCAFQGLFRR90 pKa = 11.84 NPLVSPYY97 pKa = 10.68 VLGVAAGSGFGACLAILFTEE117 pKa = 4.48 TRR119 pKa = 11.84 WIVQLSATLFGMVAVLGATGLSGIYY144 pKa = 9.75 RR145 pKa = 11.84 KK146 pKa = 10.24 ASTLVLVLGGIIVGSFFSALISLLKK171 pKa = 10.37 FQADD175 pKa = 4.07 PYY177 pKa = 9.63 EE178 pKa = 4.51 KK179 pKa = 10.38 LPAMVFWLMGSLARR193 pKa = 11.84 VSLTNLLAVAPMMAVGLGVLLALRR217 pKa = 11.84 WRR219 pKa = 11.84 LNLLAFGEE227 pKa = 4.43 QEE229 pKa = 4.06 AQALGAEE236 pKa = 4.76 VVRR239 pKa = 11.84 EE240 pKa = 3.71 RR241 pKa = 11.84 WLVILACTLLTSSAVCLAGVIGWVGLVIPHH271 pKa = 7.27 IGRR274 pKa = 11.84 LLVGPDD280 pKa = 2.97 IRR282 pKa = 11.84 RR283 pKa = 11.84 LLPASISLGSFYY295 pKa = 11.21 LLLVDD300 pKa = 3.94 TLSRR304 pKa = 11.84 TLSASEE310 pKa = 4.04 IPLGILTALIGAPFFAWLLSRR331 pKa = 11.84 NRR333 pKa = 11.84 VAWRR337 pKa = 3.38
Molecular weight: 36.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.619
IPC_protein 10.467
Toseland 10.467
ProMoST 10.423
Dawson 10.628
Bjellqvist 10.379
Wikipedia 10.847
Rodwell 10.76
Grimsley 10.687
Solomon 10.716
Lehninger 10.687
Nozaki 10.496
DTASelect 10.35
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.555
Patrickios 10.452
IPC_peptide 10.716
IPC2_peptide 9.589
IPC2.peptide.svr19 8.568
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2391
0
2391
794651
31
1932
332.4
36.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.178 ± 0.052
1.281 ± 0.019
5.015 ± 0.036
7.231 ± 0.055
3.627 ± 0.031
9.623 ± 0.048
1.841 ± 0.021
3.427 ± 0.038
3.028 ± 0.043
12.444 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.975 ± 0.02
1.996 ± 0.029
5.675 ± 0.042
2.883 ± 0.029
8.492 ± 0.061
5.13 ± 0.03
4.623 ± 0.031
8.049 ± 0.044
1.392 ± 0.019
2.089 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here