Amino acid dipepetide frequency for halophilic archaeon DL31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.096AlaAla: 13.096 ± 0.19
0.781AlaCys: 0.781 ± 0.032
8.778AlaAsp: 8.778 ± 0.132
9.017AlaGlu: 9.017 ± 0.117
3.951AlaPhe: 3.951 ± 0.073
9.351AlaGly: 9.351 ± 0.121
1.842AlaHis: 1.842 ± 0.041
4.495AlaIle: 4.495 ± 0.072
2.2AlaLys: 2.2 ± 0.044
9.748AlaLeu: 9.748 ± 0.137
2.083AlaMet: 2.083 ± 0.048
2.507AlaAsn: 2.507 ± 0.054
3.8AlaPro: 3.8 ± 0.068
2.256AlaGln: 2.256 ± 0.051
5.644AlaArg: 5.644 ± 0.085
5.115AlaSer: 5.115 ± 0.082
6.474AlaThr: 6.474 ± 0.1
10.39AlaVal: 10.39 ± 0.132
1.168AlaTrp: 1.168 ± 0.037
2.657AlaTyr: 2.657 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.584CysAla: 0.584 ± 0.025
0.095CysCys: 0.095 ± 0.011
0.567CysAsp: 0.567 ± 0.027
0.638CysGlu: 0.638 ± 0.027
0.225CysPhe: 0.225 ± 0.015
0.932CysGly: 0.932 ± 0.031
0.193CysHis: 0.193 ± 0.015
0.308CysIle: 0.308 ± 0.017
0.156CysLys: 0.156 ± 0.014
0.575CysLeu: 0.575 ± 0.025
0.106CysMet: 0.106 ± 0.012
0.197CysAsn: 0.197 ± 0.016
0.57CysPro: 0.57 ± 0.024
0.183CysGln: 0.183 ± 0.014
0.514CysArg: 0.514 ± 0.024
0.498CysSer: 0.498 ± 0.024
0.451CysThr: 0.451 ± 0.02
0.556CysVal: 0.556 ± 0.026
0.118CysTrp: 0.118 ± 0.012
0.213CysTyr: 0.213 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
9.286AspAla: 9.286 ± 0.124
0.709AspCys: 0.709 ± 0.031
6.672AspAsp: 6.672 ± 0.102
7.703AspGlu: 7.703 ± 0.117
1.821AspPhe: 1.821 ± 0.046
7.048AspGly: 7.048 ± 0.102
1.9AspHis: 1.9 ± 0.045
3.351AspIle: 3.351 ± 0.065
1.151AspLys: 1.151 ± 0.037
6.629AspLeu: 6.629 ± 0.083
1.196AspMet: 1.196 ± 0.035
1.372AspAsn: 1.372 ± 0.045
4.084AspPro: 4.084 ± 0.075
1.926AspGln: 1.926 ± 0.044
5.621AspArg: 5.621 ± 0.074
3.962AspSer: 3.962 ± 0.068
4.096AspThr: 4.096 ± 0.074
6.421AspVal: 6.421 ± 0.096
1.055AspTrp: 1.055 ± 0.03
1.8AspTyr: 1.8 ± 0.043
0.0AspXaa: 0.0 ± 0.0
Glu
9.288GluAla: 9.288 ± 0.122
0.575GluCys: 0.575 ± 0.025
5.351GluAsp: 5.351 ± 0.098
7.991GluGlu: 7.991 ± 0.144
3.318GluPhe: 3.318 ± 0.062
5.736GluGly: 5.736 ± 0.086
2.086GluHis: 2.086 ± 0.048
3.787GluIle: 3.787 ± 0.074
2.225GluLys: 2.225 ± 0.063
8.432GluLeu: 8.432 ± 0.098
1.993GluMet: 1.993 ± 0.043
2.792GluAsn: 2.792 ± 0.053
4.047GluPro: 4.047 ± 0.07
3.358GluGln: 3.358 ± 0.074
7.096GluArg: 7.096 ± 0.114
5.22GluSer: 5.22 ± 0.083
6.617GluThr: 6.617 ± 0.104
5.563GluVal: 5.563 ± 0.08
1.188GluTrp: 1.188 ± 0.038
2.742GluTyr: 2.742 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
3.643PheAla: 3.643 ± 0.075
0.291PheCys: 0.291 ± 0.02
3.212PheAsp: 3.212 ± 0.06
3.214PheGlu: 3.214 ± 0.061
1.083PhePhe: 1.083 ± 0.042
3.298PheGly: 3.298 ± 0.055
0.72PheHis: 0.72 ± 0.027
1.25PheIle: 1.25 ± 0.044
0.602PheLys: 0.602 ± 0.024
2.854PheLeu: 2.854 ± 0.07
0.519PheMet: 0.519 ± 0.025
0.795PheAsn: 0.795 ± 0.028
1.39PhePro: 1.39 ± 0.045
0.91PheGln: 0.91 ± 0.031
1.919PheArg: 1.919 ± 0.051
1.878PheSer: 1.878 ± 0.045
2.033PheThr: 2.033 ± 0.048
2.984PheVal: 2.984 ± 0.061
0.45PheTrp: 0.45 ± 0.023
0.903PheTyr: 0.903 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
7.704GlyAla: 7.704 ± 0.128
0.757GlyCys: 0.757 ± 0.027
6.146GlyAsp: 6.146 ± 0.081
7.566GlyGlu: 7.566 ± 0.103
3.151GlyPhe: 3.151 ± 0.059
7.267GlyGly: 7.267 ± 0.121
1.68GlyHis: 1.68 ± 0.046
4.113GlyIle: 4.113 ± 0.079
2.229GlyLys: 2.229 ± 0.05
7.383GlyLeu: 7.383 ± 0.114
1.721GlyMet: 1.721 ± 0.047
2.11GlyAsn: 2.11 ± 0.051
3.29GlyPro: 3.29 ± 0.058
2.182GlyGln: 2.182 ± 0.04
4.762GlyArg: 4.762 ± 0.085
5.123GlySer: 5.123 ± 0.083
5.446GlyThr: 5.446 ± 0.084
7.459GlyVal: 7.459 ± 0.101
1.118GlyTrp: 1.118 ± 0.035
2.717GlyTyr: 2.717 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.026HisAla: 2.026 ± 0.043
0.218HisCys: 0.218 ± 0.014
1.804HisAsp: 1.804 ± 0.041
1.882HisGlu: 1.882 ± 0.049
0.517HisPhe: 0.517 ± 0.023
1.939HisGly: 1.939 ± 0.052
0.546HisHis: 0.546 ± 0.029
0.858HisIle: 0.858 ± 0.03
0.412HisLys: 0.412 ± 0.021
1.77HisLeu: 1.77 ± 0.047
0.309HisMet: 0.309 ± 0.016
0.561HisAsn: 0.561 ± 0.025
1.313HisPro: 1.313 ± 0.042
0.576HisGln: 0.576 ± 0.028
1.468HisArg: 1.468 ± 0.04
0.964HisSer: 0.964 ± 0.032
1.211HisThr: 1.211 ± 0.034
1.768HisVal: 1.768 ± 0.042
0.234HisTrp: 0.234 ± 0.017
0.599HisTyr: 0.599 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
4.472IleAla: 4.472 ± 0.079
0.311IleCys: 0.311 ± 0.018
3.852IleAsp: 3.852 ± 0.06
4.301IleGlu: 4.301 ± 0.072
0.967IlePhe: 0.967 ± 0.034
3.778IleGly: 3.778 ± 0.063
0.92IleHis: 0.92 ± 0.031
1.658IleIle: 1.658 ± 0.053
0.777IleLys: 0.777 ± 0.03
2.992IleLeu: 2.992 ± 0.069
0.528IleMet: 0.528 ± 0.027
1.072IleAsn: 1.072 ± 0.038
2.212IlePro: 2.212 ± 0.058
1.323IleGln: 1.323 ± 0.038
2.55IleArg: 2.55 ± 0.057
2.343IleSer: 2.343 ± 0.055
2.463IleThr: 2.463 ± 0.054
3.463IleVal: 3.463 ± 0.066
0.345IleTrp: 0.345 ± 0.015
0.906IleTyr: 0.906 ± 0.033
0.0IleXaa: 0.0 ± 0.0
Lys
2.137LysAla: 2.137 ± 0.046
0.144LysCys: 0.144 ± 0.013
1.216LysAsp: 1.216 ± 0.036
1.772LysGlu: 1.772 ± 0.056
0.634LysPhe: 0.634 ± 0.027
1.568LysGly: 1.568 ± 0.04
0.59LysHis: 0.59 ± 0.024
0.894LysIle: 0.894 ± 0.036
0.67LysLys: 0.67 ± 0.031
2.106LysLeu: 2.106 ± 0.053
0.453LysMet: 0.453 ± 0.021
0.728LysAsn: 0.728 ± 0.034
1.096LysPro: 1.096 ± 0.032
1.04LysGln: 1.04 ± 0.034
1.837LysArg: 1.837 ± 0.052
1.319LysSer: 1.319 ± 0.044
1.518LysThr: 1.518 ± 0.039
1.316LysVal: 1.316 ± 0.039
0.26LysTrp: 0.26 ± 0.015
0.64LysTyr: 0.64 ± 0.032
0.0LysXaa: 0.0 ± 0.0
Leu
10.351LeuAla: 10.351 ± 0.142
0.632LeuCys: 0.632 ± 0.025
7.187LeuAsp: 7.187 ± 0.097
7.017LeuGlu: 7.017 ± 0.098
3.096LeuPhe: 3.096 ± 0.068
7.585LeuGly: 7.585 ± 0.112
1.703LeuHis: 1.703 ± 0.043
3.061LeuIle: 3.061 ± 0.063
1.904LeuLys: 1.904 ± 0.046
9.088LeuLeu: 9.088 ± 0.137
1.409LeuMet: 1.409 ± 0.039
2.255LeuAsn: 2.255 ± 0.056
4.376LeuPro: 4.376 ± 0.075
2.486LeuGln: 2.486 ± 0.059
5.557LeuArg: 5.557 ± 0.091
6.07LeuSer: 6.07 ± 0.082
5.608LeuThr: 5.608 ± 0.069
8.099LeuVal: 8.099 ± 0.121
0.852LeuTrp: 0.852 ± 0.03
2.198LeuTyr: 2.198 ± 0.05
0.0LeuXaa: 0.0 ± 0.0
Met
1.892MetAla: 1.892 ± 0.048
0.102MetCys: 0.102 ± 0.01
1.211MetAsp: 1.211 ± 0.041
1.253MetGlu: 1.253 ± 0.039
0.559MetPhe: 0.559 ± 0.028
1.48MetGly: 1.48 ± 0.041
0.362MetHis: 0.362 ± 0.019
0.573MetIle: 0.573 ± 0.026
0.481MetLys: 0.481 ± 0.022
1.636MetLeu: 1.636 ± 0.045
0.303MetMet: 0.303 ± 0.019
0.603MetAsn: 0.603 ± 0.024
0.844MetPro: 0.844 ± 0.031
0.588MetGln: 0.588 ± 0.026
1.129MetArg: 1.129 ± 0.036
1.623MetSer: 1.623 ± 0.04
1.439MetThr: 1.439 ± 0.04
1.354MetVal: 1.354 ± 0.043
0.17MetTrp: 0.17 ± 0.012
0.384MetTyr: 0.384 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
2.671AsnAla: 2.671 ± 0.046
0.246AsnCys: 0.246 ± 0.015
1.723AsnAsp: 1.723 ± 0.046
2.085AsnGlu: 2.085 ± 0.057
0.713AsnPhe: 0.713 ± 0.027
2.173AsnGly: 2.173 ± 0.055
0.614AsnHis: 0.614 ± 0.026
1.117AsnIle: 1.117 ± 0.039
0.564AsnLys: 0.564 ± 0.027
2.115AsnLeu: 2.115 ± 0.047
0.474AsnMet: 0.474 ± 0.024
0.725AsnAsn: 0.725 ± 0.028
1.773AsnPro: 1.773 ± 0.048
0.829AsnGln: 0.829 ± 0.031
1.858AsnArg: 1.858 ± 0.042
1.346AsnSer: 1.346 ± 0.036
1.526AsnThr: 1.526 ± 0.04
2.326AsnVal: 2.326 ± 0.056
0.343AsnTrp: 0.343 ± 0.018
0.747AsnTyr: 0.747 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
4.521ProAla: 4.521 ± 0.077
0.249ProCys: 0.249 ± 0.017
4.528ProAsp: 4.528 ± 0.066
4.895ProGlu: 4.895 ± 0.082
1.635ProPhe: 1.635 ± 0.046
3.74ProGly: 3.74 ± 0.069
0.948ProHis: 0.948 ± 0.034
1.842ProIle: 1.842 ± 0.053
1.043ProLys: 1.043 ± 0.027
3.743ProLeu: 3.743 ± 0.068
0.828ProMet: 0.828 ± 0.027
1.231ProAsn: 1.231 ± 0.04
2.163ProPro: 2.163 ± 0.049
1.136ProGln: 1.136 ± 0.031
2.227ProArg: 2.227 ± 0.05
2.768ProSer: 2.768 ± 0.053
3.24ProThr: 3.24 ± 0.062
4.088ProVal: 4.088 ± 0.075
0.523ProTrp: 0.523 ± 0.026
1.245ProTyr: 1.245 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.803GlnAla: 2.803 ± 0.052
0.175GlnCys: 0.175 ± 0.014
1.582GlnAsp: 1.582 ± 0.043
2.193GlnGlu: 2.193 ± 0.058
1.308GlnPhe: 1.308 ± 0.037
1.933GlnGly: 1.933 ± 0.045
0.651GlnHis: 0.651 ± 0.024
1.185GlnIle: 1.185 ± 0.04
0.757GlnLys: 0.757 ± 0.028
2.666GlnLeu: 2.666 ± 0.058
0.577GlnMet: 0.577 ± 0.023
0.933GlnAsn: 0.933 ± 0.035
1.38GlnPro: 1.38 ± 0.039
1.354GlnGln: 1.354 ± 0.045
2.292GlnArg: 2.292 ± 0.045
1.808GlnSer: 1.808 ± 0.04
1.755GlnThr: 1.755 ± 0.051
2.002GlnVal: 2.002 ± 0.049
0.406GlnTrp: 0.406 ± 0.022
0.972GlnTyr: 0.972 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
5.955ArgAla: 5.955 ± 0.083
0.523ArgCys: 0.523 ± 0.024
4.472ArgAsp: 4.472 ± 0.07
6.537ArgGlu: 6.537 ± 0.096
2.462ArgPhe: 2.462 ± 0.047
4.412ArgGly: 4.412 ± 0.072
1.265ArgHis: 1.265 ± 0.041
2.957ArgIle: 2.957 ± 0.058
1.598ArgLys: 1.598 ± 0.045
6.138ArgLeu: 6.138 ± 0.092
1.33ArgMet: 1.33 ± 0.039
1.865ArgAsn: 1.865 ± 0.046
2.392ArgPro: 2.392 ± 0.065
1.962ArgGln: 1.962 ± 0.043
4.499ArgArg: 4.499 ± 0.082
3.468ArgSer: 3.468 ± 0.06
3.74ArgThr: 3.74 ± 0.063
5.092ArgVal: 5.092 ± 0.08
0.751ArgTrp: 0.751 ± 0.027
1.956ArgTyr: 1.956 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
5.112SerAla: 5.112 ± 0.077
0.42SerCys: 0.42 ± 0.023
4.196SerAsp: 4.196 ± 0.074
4.722SerGlu: 4.722 ± 0.076
2.102SerPhe: 2.102 ± 0.051
5.381SerGly: 5.381 ± 0.077
1.194SerHis: 1.194 ± 0.035
2.522SerIle: 2.522 ± 0.063
1.438SerLys: 1.438 ± 0.049
5.248SerLeu: 5.248 ± 0.086
1.095SerMet: 1.095 ± 0.032
1.448SerAsn: 1.448 ± 0.043
2.807SerPro: 2.807 ± 0.053
1.666SerGln: 1.666 ± 0.045
3.369SerArg: 3.369 ± 0.066
3.186SerSer: 3.186 ± 0.065
3.554SerThr: 3.554 ± 0.064
4.825SerVal: 4.825 ± 0.074
0.686SerTrp: 0.686 ± 0.029
1.566SerTyr: 1.566 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
6.892ThrAla: 6.892 ± 0.089
0.381ThrCys: 0.381 ± 0.023
4.912ThrAsp: 4.912 ± 0.067
5.267ThrGlu: 5.267 ± 0.081
2.245ThrPhe: 2.245 ± 0.061
5.587ThrGly: 5.587 ± 0.076
1.227ThrHis: 1.227 ± 0.035
2.765ThrIle: 2.765 ± 0.058
1.287ThrLys: 1.287 ± 0.038
5.856ThrLeu: 5.856 ± 0.086
1.087ThrMet: 1.087 ± 0.031
1.621ThrAsn: 1.621 ± 0.043
3.343ThrPro: 3.343 ± 0.057
1.6ThrGln: 1.6 ± 0.043
3.209ThrArg: 3.209 ± 0.058
2.878ThrSer: 2.878 ± 0.057
4.235ThrThr: 4.235 ± 0.08
6.842ThrVal: 6.842 ± 0.112
0.635ThrTrp: 0.635 ± 0.028
1.612ThrTyr: 1.612 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
9.377ValAla: 9.377 ± 0.112
0.688ValCys: 0.688 ± 0.026
7.083ValAsp: 7.083 ± 0.092
7.831ValGlu: 7.831 ± 0.092
2.836ValPhe: 2.836 ± 0.063
7.482ValGly: 7.482 ± 0.116
1.665ValHis: 1.665 ± 0.041
3.132ValIle: 3.132 ± 0.067
1.645ValLys: 1.645 ± 0.044
7.807ValLeu: 7.807 ± 0.109
1.374ValMet: 1.374 ± 0.045
2.07ValAsn: 2.07 ± 0.047
3.952ValPro: 3.952 ± 0.069
2.129ValGln: 2.129 ± 0.049
5.037ValArg: 5.037 ± 0.076
4.948ValSer: 4.948 ± 0.074
5.715ValThr: 5.715 ± 0.086
8.206ValVal: 8.206 ± 0.123
0.799ValTrp: 0.799 ± 0.029
2.021ValTyr: 2.021 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.972TrpAla: 0.972 ± 0.032
0.116TrpCys: 0.116 ± 0.012
0.753TrpAsp: 0.753 ± 0.027
0.981TrpGlu: 0.981 ± 0.033
0.462TrpPhe: 0.462 ± 0.024
0.901TrpGly: 0.901 ± 0.03
0.236TrpHis: 0.236 ± 0.015
0.432TrpIle: 0.432 ± 0.024
0.326TrpLys: 0.326 ± 0.017
1.258TrpLeu: 1.258 ± 0.035
0.232TrpMet: 0.232 ± 0.015
0.391TrpAsn: 0.391 ± 0.019
0.432TrpPro: 0.432 ± 0.02
0.406TrpGln: 0.406 ± 0.021
0.848TrpArg: 0.848 ± 0.029
0.641TrpSer: 0.641 ± 0.025
0.798TrpThr: 0.798 ± 0.029
0.899TrpVal: 0.899 ± 0.032
0.214TrpTrp: 0.214 ± 0.015
0.365TrpTyr: 0.365 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.571TyrAla: 2.571 ± 0.06
0.265TyrCys: 0.265 ± 0.015
2.558TyrAsp: 2.558 ± 0.055
2.539TyrGlu: 2.539 ± 0.046
0.864TyrPhe: 0.864 ± 0.028
2.333TyrGly: 2.333 ± 0.058
0.632TyrHis: 0.632 ± 0.026
0.934TyrIle: 0.934 ± 0.036
0.469TyrLys: 0.469 ± 0.024
2.489TyrLeu: 2.489 ± 0.052
0.371TyrMet: 0.371 ± 0.022
0.689TyrAsn: 0.689 ± 0.027
1.327TyrPro: 1.327 ± 0.036
0.822TyrGln: 0.822 ± 0.03
1.94TyrArg: 1.94 ± 0.047
1.376TyrSer: 1.376 ± 0.038
1.565TyrThr: 1.565 ± 0.036
2.148TyrVal: 2.148 ± 0.051
0.351TyrTrp: 0.351 ± 0.019
0.839TyrTyr: 0.839 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3413 proteins (980990 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski