Corynebacterium sp. KPL1855
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2285 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U7L100|U7L100_9CORY Uncharacterized protein OS=Corynebacterium sp. KPL1855 OX=1203562 GN=HMPREF1281_01714 PE=4 SV=1
MM1 pKa = 7.26 KK2 pKa = 10.39 RR3 pKa = 11.84 SITLAALVLTSTLALTACSDD23 pKa = 3.52 ATDD26 pKa = 4.01 NSDD29 pKa = 5.03 DD30 pKa = 4.68 ADD32 pKa = 4.04 TTSTTTATAEE42 pKa = 4.19 TNEE45 pKa = 4.09 THH47 pKa = 7.0 QEE49 pKa = 3.92 DD50 pKa = 4.48 TTTADD55 pKa = 3.85 EE56 pKa = 4.09 EE57 pKa = 4.89 HH58 pKa = 6.92 GGHH61 pKa = 6.94 DD62 pKa = 3.56 HH63 pKa = 7.03 PADD66 pKa = 4.04 GGAPPAGIEE75 pKa = 4.02 EE76 pKa = 4.9 AEE78 pKa = 4.43 DD79 pKa = 3.24 PTYY82 pKa = 10.65 PVGTEE87 pKa = 4.03 VILTADD93 pKa = 4.44 HH94 pKa = 6.28 MPGMDD99 pKa = 3.64 GATATISGAFDD110 pKa = 3.15 TTTYY114 pKa = 10.41 SVSYY118 pKa = 9.66 TPAEE122 pKa = 4.29 GGAPVTDD129 pKa = 3.89 HH130 pKa = 6.43 RR131 pKa = 11.84 WVVHH135 pKa = 6.18 EE136 pKa = 4.48 EE137 pKa = 3.95 LVDD140 pKa = 4.02 PGQAPLPDD148 pKa = 4.18 GASVVLDD155 pKa = 3.87 AEE157 pKa = 4.78 HH158 pKa = 6.21 MSGMKK163 pKa = 10.11 GAEE166 pKa = 3.73 ATIDD170 pKa = 3.59 YY171 pKa = 8.79 STEE174 pKa = 3.61 EE175 pKa = 3.86 TVYY178 pKa = 10.39 MVDD181 pKa = 3.49 LTVDD185 pKa = 3.48 GMTMTNHH192 pKa = 5.38 KK193 pKa = 9.32 WVTEE197 pKa = 4.09 SEE199 pKa = 4.23 IQPAEE204 pKa = 3.8
Molecular weight: 21.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.656
ProMoST 3.986
Dawson 3.834
Bjellqvist 4.012
Wikipedia 3.745
Rodwell 3.681
Grimsley 3.567
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.151
Thurlkill 3.694
EMBOSS 3.757
Sillero 3.973
Patrickios 1.888
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.89
Protein with the highest isoelectric point:
>tr|U7L7B6|U7L7B6_9CORY Peptide chain release factor 2 OS=Corynebacterium sp. KPL1855 OX=1203562 GN=prfB PE=3 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 KK18 pKa = 8.59 HH19 pKa = 4.58 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MSTRR28 pKa = 11.84 SGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.71 KK40 pKa = 10.51 GRR42 pKa = 11.84 AKK44 pKa = 10.73 LSAA47 pKa = 3.92
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2285
0
2285
719998
24
1642
315.1
34.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.467 ± 0.058
0.708 ± 0.015
6.2 ± 0.043
6.565 ± 0.054
3.38 ± 0.032
8.282 ± 0.045
2.19 ± 0.022
5.243 ± 0.039
3.618 ± 0.038
9.371 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.278 ± 0.024
2.978 ± 0.03
4.919 ± 0.034
3.444 ± 0.029
5.976 ± 0.052
6.05 ± 0.039
5.941 ± 0.032
7.69 ± 0.045
1.409 ± 0.021
2.29 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here