Pseudogymnoascus sp. 05NY08

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10300 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B8EIP2|A0A1B8EIP2_9PEZI Kinetochore protein SPC25 OS=Pseudogymnoascus sp. 05NY08 OX=1622149 GN=VF21_09894 PE=3 SV=1
MM1 pKa = 7.81EE2 pKa = 4.44GTICGDD8 pKa = 3.41IPKK11 pKa = 9.67HH12 pKa = 6.03SPLARR17 pKa = 11.84SSCSIKK23 pKa = 10.37LAPNVACCSWDD34 pKa = 3.56VFSVDD39 pKa = 3.57NWDD42 pKa = 3.68QVDD45 pKa = 4.08DD46 pKa = 4.28NVPVANDD53 pKa = 2.98IASFIVSSDD62 pKa = 3.48SALVCADD69 pKa = 4.66LEE71 pKa = 4.65DD72 pKa = 4.23SDD74 pKa = 4.64GAINAGIIGTDD85 pKa = 3.1NDD87 pKa = 3.74NQEE90 pKa = 4.71DD91 pKa = 5.55VYY93 pKa = 11.19TDD95 pKa = 3.52TYY97 pKa = 10.67LQEE100 pKa = 5.41ALDD103 pKa = 4.18FCAKK107 pKa = 10.54GDD109 pKa = 3.95GLKK112 pKa = 10.7KK113 pKa = 10.63LL114 pKa = 4.02

Molecular weight: 12.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B8ES18|A0A1B8ES18_9PEZI Alginate_lyase domain-containing protein OS=Pseudogymnoascus sp. 05NY08 OX=1622149 GN=VF21_06232 PE=3 SV=1
MM1 pKa = 7.38AAAGPSRR8 pKa = 11.84EE9 pKa = 4.47EE10 pKa = 3.7EE11 pKa = 4.72DD12 pKa = 2.97ISPRR16 pKa = 11.84RR17 pKa = 11.84SYY19 pKa = 11.73AMEE22 pKa = 3.71AHH24 pKa = 6.19EE25 pKa = 4.13LARR28 pKa = 11.84YY29 pKa = 9.12RR30 pKa = 11.84SQYY33 pKa = 11.29SDD35 pKa = 2.86ATSAHH40 pKa = 5.94HH41 pKa = 6.31RR42 pKa = 11.84ASSRR46 pKa = 11.84ARR48 pKa = 11.84SRR50 pKa = 11.84KK51 pKa = 9.09PLGPPKK57 pKa = 10.54GLARR61 pKa = 11.84VPFEE65 pKa = 4.74LKK67 pKa = 10.61KK68 pKa = 10.41FWGRR72 pKa = 11.84QISVIVDD79 pKa = 3.23QAHH82 pKa = 5.99NRR84 pKa = 11.84DD85 pKa = 3.61HH86 pKa = 7.04LALEE90 pKa = 4.45RR91 pKa = 11.84TFLGYY96 pKa = 10.71LRR98 pKa = 11.84TSQALSMLGVIISQLFTLQKK118 pKa = 9.38STSPDD123 pKa = 2.97PHH125 pKa = 7.36IGYY128 pKa = 9.33FVTGKK133 pKa = 9.46PLGAICQGAAMLTLMLGTFRR153 pKa = 11.84WWRR156 pKa = 11.84QQNAITRR163 pKa = 11.84GKK165 pKa = 9.9ALAGGFEE172 pKa = 4.01ITVIGLLVLLLCIVLFGILVAIEE195 pKa = 3.63IRR197 pKa = 11.84DD198 pKa = 3.6FRR200 pKa = 11.84RR201 pKa = 11.84RR202 pKa = 11.84LL203 pKa = 3.28

Molecular weight: 22.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10300

0

10300

5075312

51

5717

492.7

54.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.984 ± 0.024

1.154 ± 0.01

5.6 ± 0.015

6.36 ± 0.027

3.654 ± 0.015

7.386 ± 0.023

2.194 ± 0.011

4.993 ± 0.017

5.021 ± 0.024

8.699 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.009

3.704 ± 0.014

5.979 ± 0.025

3.811 ± 0.02

5.797 ± 0.022

8.046 ± 0.025

6.061 ± 0.018

6.192 ± 0.019

1.411 ± 0.008

2.758 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski