Pseudomonas phage ventosus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 172 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4P7Z1|A0A2H4P7Z1_9CAUD Uncharacterized protein OS=Pseudomonas phage ventosus OX=2048980 GN=CNR37_00096 PE=4 SV=1
MM1 pKa = 7.22FALILSLCINATSCNDD17 pKa = 3.38YY18 pKa = 11.13VIDD21 pKa = 3.98SSPVEE26 pKa = 4.23GDD28 pKa = 3.49CHH30 pKa = 7.68SMLVEE35 pKa = 4.37RR36 pKa = 11.84SDD38 pKa = 3.81SFADD42 pKa = 3.29AWADD46 pKa = 3.31DD47 pKa = 4.14DD48 pKa = 5.29ANKK51 pKa = 10.46KK52 pKa = 8.89LAEE55 pKa = 3.88WLKK58 pKa = 9.92PYY60 pKa = 10.4KK61 pKa = 9.82IVEE64 pKa = 4.7PIEE67 pKa = 4.12RR68 pKa = 11.84VSMYY72 pKa = 11.05DD73 pKa = 3.08FTCPFIPDD81 pKa = 3.11SDD83 pKa = 3.84IPP85 pKa = 3.79

Molecular weight: 9.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4P809|A0A2H4P809_9CAUD Uncharacterized protein OS=Pseudomonas phage ventosus OX=2048980 GN=CNR37_00091 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 9.98TMKK5 pKa = 10.41PMMYY9 pKa = 10.22FVGNMAKK16 pKa = 10.42LLSVWEE22 pKa = 4.12HH23 pKa = 6.76SILAMLPCTTLILLVRR39 pKa = 11.84STLVRR44 pKa = 11.84WQSTGVARR52 pKa = 4.91

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

172

0

172

28064

33

870

163.2

18.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.523 ± 0.223

1.265 ± 0.106

5.947 ± 0.156

6.727 ± 0.224

4.144 ± 0.144

7.458 ± 0.244

1.96 ± 0.131

5.509 ± 0.158

6.143 ± 0.206

7.914 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.054 ± 0.121

4.208 ± 0.169

3.756 ± 0.164

3.955 ± 0.132

5.106 ± 0.187

5.587 ± 0.211

6.336 ± 0.228

7.283 ± 0.229

1.55 ± 0.102

3.574 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski