Amino acid dipepetide frequency for Pseudomonas phage ventosus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.837AlaAla: 8.837 ± 0.73
1.14AlaCys: 1.14 ± 0.228
3.706AlaAsp: 3.706 ± 0.438
5.06AlaGlu: 5.06 ± 0.466
3.421AlaPhe: 3.421 ± 0.387
6.236AlaGly: 6.236 ± 0.495
1.924AlaHis: 1.924 ± 0.291
5.309AlaIle: 5.309 ± 0.396
4.989AlaLys: 4.989 ± 0.474
7.234AlaLeu: 7.234 ± 0.52
3.278AlaMet: 3.278 ± 0.399
3.314AlaAsn: 3.314 ± 0.304
2.708AlaPro: 2.708 ± 0.319
3.92AlaGln: 3.92 ± 0.351
4.347AlaArg: 4.347 ± 0.445
4.205AlaSer: 4.205 ± 0.466
6.236AlaThr: 6.236 ± 0.491
5.452AlaVal: 5.452 ± 0.573
1.212AlaTrp: 1.212 ± 0.214
2.708AlaTyr: 2.708 ± 0.282
0.0AlaXaa: 0.0 ± 0.0
Cys
0.748CysAla: 0.748 ± 0.187
0.214CysCys: 0.214 ± 0.08
0.962CysAsp: 0.962 ± 0.183
0.855CysGlu: 0.855 ± 0.152
0.855CysPhe: 0.855 ± 0.175
0.962CysGly: 0.962 ± 0.211
0.535CysHis: 0.535 ± 0.164
0.926CysIle: 0.926 ± 0.175
0.962CysLys: 0.962 ± 0.205
0.748CysLeu: 0.748 ± 0.183
0.463CysMet: 0.463 ± 0.135
0.57CysAsn: 0.57 ± 0.167
0.535CysPro: 0.535 ± 0.127
0.356CysGln: 0.356 ± 0.119
0.499CysArg: 0.499 ± 0.138
0.535CysSer: 0.535 ± 0.119
0.606CysThr: 0.606 ± 0.148
0.748CysVal: 0.748 ± 0.17
0.036CysTrp: 0.036 ± 0.033
0.535CysTyr: 0.535 ± 0.135
0.0CysXaa: 0.0 ± 0.0
Asp
5.167AspAla: 5.167 ± 0.434
0.855AspCys: 0.855 ± 0.203
3.67AspAsp: 3.67 ± 0.398
3.207AspGlu: 3.207 ± 0.29
2.566AspPhe: 2.566 ± 0.316
5.238AspGly: 5.238 ± 0.401
1.176AspHis: 1.176 ± 0.208
3.492AspIle: 3.492 ± 0.364
3.385AspLys: 3.385 ± 0.335
4.668AspLeu: 4.668 ± 0.41
1.924AspMet: 1.924 ± 0.233
2.744AspAsn: 2.744 ± 0.286
2.209AspPro: 2.209 ± 0.241
2.102AspGln: 2.102 ± 0.245
2.53AspArg: 2.53 ± 0.378
3.385AspSer: 3.385 ± 0.295
3.599AspThr: 3.599 ± 0.324
4.098AspVal: 4.098 ± 0.376
1.318AspTrp: 1.318 ± 0.206
2.138AspTyr: 2.138 ± 0.265
0.0AspXaa: 0.0 ± 0.0
Glu
5.844GluAla: 5.844 ± 0.453
0.855GluCys: 0.855 ± 0.184
3.92GluAsp: 3.92 ± 0.431
5.381GluGlu: 5.381 ± 0.558
3.35GluPhe: 3.35 ± 0.349
4.347GluGly: 4.347 ± 0.427
1.497GluHis: 1.497 ± 0.233
3.314GluIle: 3.314 ± 0.346
4.24GluLys: 4.24 ± 0.454
6.022GluLeu: 6.022 ± 0.479
2.494GluMet: 2.494 ± 0.296
1.996GluAsn: 1.996 ± 0.256
1.71GluPro: 1.71 ± 0.264
3.35GluGln: 3.35 ± 0.406
3.777GluArg: 3.777 ± 0.463
2.815GluSer: 2.815 ± 0.277
3.457GluThr: 3.457 ± 0.342
5.167GluVal: 5.167 ± 0.455
1.247GluTrp: 1.247 ± 0.215
2.494GluTyr: 2.494 ± 0.304
0.0GluXaa: 0.0 ± 0.0
Phe
3.171PheAla: 3.171 ± 0.356
0.57PheCys: 0.57 ± 0.134
2.494PheAsp: 2.494 ± 0.31
3.528PheGlu: 3.528 ± 0.359
1.71PhePhe: 1.71 ± 0.263
3.35PheGly: 3.35 ± 0.378
0.677PheHis: 0.677 ± 0.127
2.779PheIle: 2.779 ± 0.336
2.387PheLys: 2.387 ± 0.264
2.993PheLeu: 2.993 ± 0.275
1.318PheMet: 1.318 ± 0.239
1.889PheAsn: 1.889 ± 0.249
1.283PhePro: 1.283 ± 0.214
1.675PheGln: 1.675 ± 0.253
1.746PheArg: 1.746 ± 0.274
2.316PheSer: 2.316 ± 0.283
2.851PheThr: 2.851 ± 0.309
2.922PheVal: 2.922 ± 0.293
0.606PheTrp: 0.606 ± 0.169
1.176PheTyr: 1.176 ± 0.211
0.0PheXaa: 0.0 ± 0.0
Gly
4.739GlyAla: 4.739 ± 0.537
0.926GlyCys: 0.926 ± 0.172
4.775GlyAsp: 4.775 ± 0.509
4.383GlyGlu: 4.383 ± 0.425
3.1GlyPhe: 3.1 ± 0.334
5.987GlyGly: 5.987 ± 0.648
1.212GlyHis: 1.212 ± 0.208
4.276GlyIle: 4.276 ± 0.401
4.882GlyLys: 4.882 ± 0.518
5.808GlyLeu: 5.808 ± 0.48
1.996GlyMet: 1.996 ± 0.315
3.136GlyAsn: 3.136 ± 0.365
1.425GlyPro: 1.425 ± 0.256
3.171GlyGln: 3.171 ± 0.493
3.991GlyArg: 3.991 ± 0.352
4.882GlySer: 4.882 ± 0.506
4.918GlyThr: 4.918 ± 0.628
6.307GlyVal: 6.307 ± 0.519
1.247GlyTrp: 1.247 ± 0.201
3.421GlyTyr: 3.421 ± 0.446
0.0GlyXaa: 0.0 ± 0.0
His
1.71HisAla: 1.71 ± 0.201
0.535HisCys: 0.535 ± 0.142
1.212HisAsp: 1.212 ± 0.208
1.14HisGlu: 1.14 ± 0.205
0.748HisPhe: 0.748 ± 0.171
1.675HisGly: 1.675 ± 0.224
0.285HisHis: 0.285 ± 0.138
1.033HisIle: 1.033 ± 0.166
1.283HisLys: 1.283 ± 0.27
1.497HisLeu: 1.497 ± 0.224
0.57HisMet: 0.57 ± 0.139
0.891HisAsn: 0.891 ± 0.171
0.784HisPro: 0.784 ± 0.178
0.392HisGln: 0.392 ± 0.137
0.82HisArg: 0.82 ± 0.19
0.926HisSer: 0.926 ± 0.176
1.568HisThr: 1.568 ± 0.217
1.318HisVal: 1.318 ± 0.226
0.392HisTrp: 0.392 ± 0.148
0.82HisTyr: 0.82 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
4.276IleAla: 4.276 ± 0.4
0.713IleCys: 0.713 ± 0.177
3.991IleAsp: 3.991 ± 0.367
4.526IleGlu: 4.526 ± 0.419
1.817IlePhe: 1.817 ± 0.256
4.205IleGly: 4.205 ± 0.41
1.033IleHis: 1.033 ± 0.174
2.316IleIle: 2.316 ± 0.284
3.706IleLys: 3.706 ± 0.354
3.421IleLeu: 3.421 ± 0.29
1.532IleMet: 1.532 ± 0.275
2.779IleAsn: 2.779 ± 0.354
2.494IlePro: 2.494 ± 0.252
1.96IleGln: 1.96 ± 0.237
2.958IleArg: 2.958 ± 0.375
3.065IleSer: 3.065 ± 0.329
3.955IleThr: 3.955 ± 0.465
3.955IleVal: 3.955 ± 0.407
0.748IleTrp: 0.748 ± 0.186
1.639IleTyr: 1.639 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
6.307LysAla: 6.307 ± 0.553
0.748LysCys: 0.748 ± 0.158
3.563LysAsp: 3.563 ± 0.424
5.238LysGlu: 5.238 ± 0.44
2.281LysPhe: 2.281 ± 0.329
4.561LysGly: 4.561 ± 0.405
1.318LysHis: 1.318 ± 0.221
2.993LysIle: 2.993 ± 0.324
2.601LysLys: 2.601 ± 0.314
5.06LysLeu: 5.06 ± 0.408
2.174LysMet: 2.174 ± 0.26
1.532LysAsn: 1.532 ± 0.297
2.459LysPro: 2.459 ± 0.331
2.566LysGln: 2.566 ± 0.276
3.314LysArg: 3.314 ± 0.327
3.278LysSer: 3.278 ± 0.395
3.385LysThr: 3.385 ± 0.41
4.918LysVal: 4.918 ± 0.433
1.033LysTrp: 1.033 ± 0.154
2.067LysTyr: 2.067 ± 0.249
0.0LysXaa: 0.0 ± 0.0
Leu
7.483LeuAla: 7.483 ± 0.576
1.069LeuCys: 1.069 ± 0.223
5.452LeuAsp: 5.452 ± 0.457
5.024LeuGlu: 5.024 ± 0.405
3.065LeuPhe: 3.065 ± 0.317
5.808LeuGly: 5.808 ± 0.492
1.283LeuHis: 1.283 ± 0.197
4.276LeuIle: 4.276 ± 0.411
4.918LeuLys: 4.918 ± 0.411
5.987LeuLeu: 5.987 ± 0.501
2.566LeuMet: 2.566 ± 0.278
3.563LeuAsn: 3.563 ± 0.422
3.742LeuPro: 3.742 ± 0.418
3.314LeuGln: 3.314 ± 0.376
3.777LeuArg: 3.777 ± 0.344
4.704LeuSer: 4.704 ± 0.363
4.597LeuThr: 4.597 ± 0.407
5.06LeuVal: 5.06 ± 0.41
0.998LeuTrp: 0.998 ± 0.168
2.459LeuTyr: 2.459 ± 0.304
0.0LeuXaa: 0.0 ± 0.0
Met
3.136MetAla: 3.136 ± 0.377
0.356MetCys: 0.356 ± 0.138
1.425MetAsp: 1.425 ± 0.239
1.746MetGlu: 1.746 ± 0.258
0.855MetPhe: 0.855 ± 0.199
1.71MetGly: 1.71 ± 0.271
0.499MetHis: 0.499 ± 0.13
1.425MetIle: 1.425 ± 0.229
2.637MetLys: 2.637 ± 0.291
2.566MetLeu: 2.566 ± 0.295
0.641MetMet: 0.641 ± 0.148
1.283MetAsn: 1.283 ± 0.247
1.14MetPro: 1.14 ± 0.173
1.354MetGln: 1.354 ± 0.195
1.283MetArg: 1.283 ± 0.255
2.067MetSer: 2.067 ± 0.299
2.779MetThr: 2.779 ± 0.336
2.387MetVal: 2.387 ± 0.261
0.249MetTrp: 0.249 ± 0.083
0.998MetTyr: 0.998 ± 0.209
0.0MetXaa: 0.0 ± 0.0
Asn
3.1AsnAla: 3.1 ± 0.354
0.57AsnCys: 0.57 ± 0.143
1.996AsnAsp: 1.996 ± 0.275
1.746AsnGlu: 1.746 ± 0.284
1.604AsnPhe: 1.604 ± 0.286
4.169AsnGly: 4.169 ± 0.381
0.784AsnHis: 0.784 ± 0.149
2.566AsnIle: 2.566 ± 0.319
2.958AsnLys: 2.958 ± 0.309
3.563AsnLeu: 3.563 ± 0.461
0.677AsnMet: 0.677 ± 0.151
1.924AsnAsn: 1.924 ± 0.296
2.494AsnPro: 2.494 ± 0.315
1.212AsnGln: 1.212 ± 0.204
2.245AsnArg: 2.245 ± 0.307
2.53AsnSer: 2.53 ± 0.328
2.637AsnThr: 2.637 ± 0.317
3.243AsnVal: 3.243 ± 0.379
0.641AsnTrp: 0.641 ± 0.161
1.425AsnTyr: 1.425 ± 0.235
0.0AsnXaa: 0.0 ± 0.0
Pro
3.243ProAla: 3.243 ± 0.322
0.285ProCys: 0.285 ± 0.105
2.316ProAsp: 2.316 ± 0.281
3.563ProGlu: 3.563 ± 0.363
1.71ProPhe: 1.71 ± 0.215
2.031ProGly: 2.031 ± 0.324
0.926ProHis: 0.926 ± 0.157
1.354ProIle: 1.354 ± 0.212
2.174ProLys: 2.174 ± 0.288
3.457ProLeu: 3.457 ± 0.328
1.247ProMet: 1.247 ± 0.186
1.425ProAsn: 1.425 ± 0.199
1.176ProPro: 1.176 ± 0.235
1.283ProGln: 1.283 ± 0.239
1.782ProArg: 1.782 ± 0.258
2.352ProSer: 2.352 ± 0.353
2.174ProThr: 2.174 ± 0.315
2.993ProVal: 2.993 ± 0.362
0.677ProTrp: 0.677 ± 0.207
1.39ProTyr: 1.39 ± 0.259
0.0ProXaa: 0.0 ± 0.0
Gln
3.563GlnAla: 3.563 ± 0.426
0.57GlnCys: 0.57 ± 0.147
2.067GlnAsp: 2.067 ± 0.258
2.53GlnGlu: 2.53 ± 0.354
2.067GlnPhe: 2.067 ± 0.293
2.494GlnGly: 2.494 ± 0.266
0.926GlnHis: 0.926 ± 0.192
2.102GlnIle: 2.102 ± 0.237
2.138GlnLys: 2.138 ± 0.3
3.742GlnLeu: 3.742 ± 0.354
1.354GlnMet: 1.354 ± 0.235
1.212GlnAsn: 1.212 ± 0.208
1.532GlnPro: 1.532 ± 0.237
1.532GlnGln: 1.532 ± 0.233
2.174GlnArg: 2.174 ± 0.234
2.209GlnSer: 2.209 ± 0.287
1.782GlnThr: 1.782 ± 0.273
3.385GlnVal: 3.385 ± 0.329
0.57GlnTrp: 0.57 ± 0.132
1.604GlnTyr: 1.604 ± 0.263
0.0GlnXaa: 0.0 ± 0.0
Arg
3.92ArgAla: 3.92 ± 0.348
0.428ArgCys: 0.428 ± 0.154
3.528ArgAsp: 3.528 ± 0.39
3.136ArgGlu: 3.136 ± 0.357
2.281ArgPhe: 2.281 ± 0.263
3.243ArgGly: 3.243 ± 0.389
1.212ArgHis: 1.212 ± 0.224
2.851ArgIle: 2.851 ± 0.294
2.637ArgLys: 2.637 ± 0.337
4.134ArgLeu: 4.134 ± 0.417
1.639ArgMet: 1.639 ± 0.252
2.316ArgAsn: 2.316 ± 0.299
1.96ArgPro: 1.96 ± 0.342
1.746ArgGln: 1.746 ± 0.239
2.993ArgArg: 2.993 ± 0.375
2.851ArgSer: 2.851 ± 0.322
3.421ArgThr: 3.421 ± 0.335
3.528ArgVal: 3.528 ± 0.318
1.283ArgTrp: 1.283 ± 0.241
1.96ArgTyr: 1.96 ± 0.264
0.0ArgXaa: 0.0 ± 0.0
Ser
4.561SerAla: 4.561 ± 0.52
0.606SerCys: 0.606 ± 0.16
2.958SerAsp: 2.958 ± 0.282
3.207SerGlu: 3.207 ± 0.374
2.744SerPhe: 2.744 ± 0.318
4.989SerGly: 4.989 ± 0.411
0.962SerHis: 0.962 ± 0.175
2.958SerIle: 2.958 ± 0.352
3.029SerLys: 3.029 ± 0.313
4.383SerLeu: 4.383 ± 0.474
1.568SerMet: 1.568 ± 0.235
3.029SerAsn: 3.029 ± 0.377
2.174SerPro: 2.174 ± 0.261
2.209SerGln: 2.209 ± 0.297
3.207SerArg: 3.207 ± 0.45
3.742SerSer: 3.742 ± 0.675
3.065SerThr: 3.065 ± 0.396
3.635SerVal: 3.635 ± 0.383
0.926SerTrp: 0.926 ± 0.171
1.924SerTyr: 1.924 ± 0.256
0.0SerXaa: 0.0 ± 0.0
Thr
5.523ThrAla: 5.523 ± 0.469
0.641ThrCys: 0.641 ± 0.169
3.314ThrAsp: 3.314 ± 0.32
4.347ThrGlu: 4.347 ± 0.402
2.387ThrPhe: 2.387 ± 0.253
5.096ThrGly: 5.096 ± 0.646
1.283ThrHis: 1.283 ± 0.217
4.454ThrIle: 4.454 ± 0.395
3.528ThrLys: 3.528 ± 0.305
4.846ThrLeu: 4.846 ± 0.38
1.461ThrMet: 1.461 ± 0.195
2.673ThrAsn: 2.673 ± 0.333
3.243ThrPro: 3.243 ± 0.375
2.779ThrGln: 2.779 ± 0.261
3.029ThrArg: 3.029 ± 0.338
3.171ThrSer: 3.171 ± 0.337
3.67ThrThr: 3.67 ± 0.365
4.846ThrVal: 4.846 ± 0.438
0.641ThrTrp: 0.641 ± 0.185
2.423ThrTyr: 2.423 ± 0.297
0.0ThrXaa: 0.0 ± 0.0
Val
6.272ValAla: 6.272 ± 0.562
0.748ValCys: 0.748 ± 0.182
4.989ValAsp: 4.989 ± 0.334
5.452ValGlu: 5.452 ± 0.481
2.815ValPhe: 2.815 ± 0.351
4.811ValGly: 4.811 ± 0.509
1.033ValHis: 1.033 ± 0.166
3.243ValIle: 3.243 ± 0.299
5.167ValLys: 5.167 ± 0.491
5.06ValLeu: 5.06 ± 0.432
2.281ValMet: 2.281 ± 0.323
3.457ValAsn: 3.457 ± 0.317
2.53ValPro: 2.53 ± 0.323
2.922ValGln: 2.922 ± 0.264
3.385ValArg: 3.385 ± 0.388
3.884ValSer: 3.884 ± 0.391
5.131ValThr: 5.131 ± 0.484
5.915ValVal: 5.915 ± 0.457
0.926ValTrp: 0.926 ± 0.188
2.815ValTyr: 2.815 ± 0.27
0.0ValXaa: 0.0 ± 0.0
Trp
0.891TrpAla: 0.891 ± 0.193
0.214TrpCys: 0.214 ± 0.083
1.069TrpAsp: 1.069 ± 0.186
1.318TrpGlu: 1.318 ± 0.239
0.57TrpPhe: 0.57 ± 0.145
0.926TrpGly: 0.926 ± 0.172
0.428TrpHis: 0.428 ± 0.115
1.069TrpIle: 1.069 ± 0.202
0.891TrpLys: 0.891 ± 0.194
1.639TrpLeu: 1.639 ± 0.256
0.428TrpMet: 0.428 ± 0.124
0.891TrpAsn: 0.891 ± 0.176
0.428TrpPro: 0.428 ± 0.123
0.713TrpGln: 0.713 ± 0.161
0.82TrpArg: 0.82 ± 0.156
0.677TrpSer: 0.677 ± 0.154
0.606TrpThr: 0.606 ± 0.131
0.926TrpVal: 0.926 ± 0.167
0.356TrpTrp: 0.356 ± 0.085
0.641TrpTyr: 0.641 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.744TyrAla: 2.744 ± 0.336
0.606TyrCys: 0.606 ± 0.211
2.067TyrAsp: 2.067 ± 0.263
1.889TyrGlu: 1.889 ± 0.304
1.497TyrPhe: 1.497 ± 0.205
2.744TyrGly: 2.744 ± 0.322
0.606TyrHis: 0.606 ± 0.181
2.352TyrIle: 2.352 ± 0.325
2.922TyrLys: 2.922 ± 0.358
2.316TyrLeu: 2.316 ± 0.317
0.891TyrMet: 0.891 ± 0.15
1.461TyrAsn: 1.461 ± 0.229
1.532TyrPro: 1.532 ± 0.245
0.998TyrGln: 0.998 ± 0.215
2.387TyrArg: 2.387 ± 0.3
2.281TyrSer: 2.281 ± 0.364
2.922TyrThr: 2.922 ± 0.287
2.031TyrVal: 2.031 ± 0.218
0.392TyrTrp: 0.392 ± 0.123
1.105TyrTyr: 1.105 ± 0.254
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 172 proteins (28064 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski