Desulfonema ishimotonii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfonema

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5012 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A401FWM4|A0A401FWM4_9DELT ABC transporter ATP-binding protein OS=Desulfonema ishimotonii OX=45657 GN=DENIS_2337 PE=4 SV=1
MM1 pKa = 7.49TSGEE5 pKa = 4.48YY6 pKa = 8.87FTVRR10 pKa = 11.84YY11 pKa = 9.28EE12 pKa = 4.07SGGVKK17 pKa = 10.16LVVSEE22 pKa = 4.71VISEE26 pKa = 3.86FSIGLTISPSGSGRR40 pKa = 11.84VLIEE44 pKa = 3.86EE45 pKa = 4.52EE46 pKa = 4.46GDD48 pKa = 3.8GEE50 pKa = 4.53TGTTGLTFTCPDD62 pKa = 3.76DD63 pKa = 5.52CVDD66 pKa = 5.77SDD68 pKa = 4.34TEE70 pKa = 4.43CNSGFCKK77 pKa = 10.36KK78 pKa = 10.17YY79 pKa = 9.06FAPGARR85 pKa = 11.84LKK87 pKa = 10.0LTATPGSGYY96 pKa = 10.75LFDD99 pKa = 3.57TWDD102 pKa = 3.98GEE104 pKa = 4.66VEE106 pKa = 4.32STSDD110 pKa = 3.41PKK112 pKa = 10.61IVYY115 pKa = 10.22VDD117 pKa = 3.68MAADD121 pKa = 3.4QSVTARR127 pKa = 11.84FEE129 pKa = 3.96ISQPLPDD136 pKa = 4.6RR137 pKa = 11.84DD138 pKa = 3.9GDD140 pKa = 4.15GTPDD144 pKa = 3.69TQDD147 pKa = 3.22GCPDD151 pKa = 3.98DD152 pKa = 4.16PRR154 pKa = 11.84KK155 pKa = 8.59TSPGACGCGVADD167 pKa = 4.16TDD169 pKa = 4.27SDD171 pKa = 3.91GDD173 pKa = 3.87EE174 pKa = 4.57TPDD177 pKa = 5.25CNDD180 pKa = 3.7EE181 pKa = 4.98CPADD185 pKa = 3.99PNKK188 pKa = 9.52TAPGQCGCGVADD200 pKa = 4.02TDD202 pKa = 4.27SDD204 pKa = 3.91GDD206 pKa = 3.87EE207 pKa = 4.57TPDD210 pKa = 5.29CNDD213 pKa = 3.9EE214 pKa = 5.15CPDD217 pKa = 4.71DD218 pKa = 4.48PDD220 pKa = 3.64KK221 pKa = 11.16TSPGACGCGVADD233 pKa = 4.24TDD235 pKa = 4.16SDD237 pKa = 3.8GDD239 pKa = 3.88ATPDD243 pKa = 3.93CNDD246 pKa = 3.99RR247 pKa = 11.84CPDD250 pKa = 4.29DD251 pKa = 4.24PDD253 pKa = 3.42KK254 pKa = 11.38TEE256 pKa = 5.01PGQCGCGLSDD266 pKa = 4.99ADD268 pKa = 3.92SDD270 pKa = 4.09GDD272 pKa = 4.02GFSDD276 pKa = 5.87CRR278 pKa = 11.84DD279 pKa = 3.41QCPDD283 pKa = 4.01DD284 pKa = 4.49PGKK287 pKa = 8.32TLPGQCGCGVADD299 pKa = 4.43TDD301 pKa = 3.74TDD303 pKa = 3.93GDD305 pKa = 4.01GTADD309 pKa = 4.9CNDD312 pKa = 4.02KK313 pKa = 11.53CPDD316 pKa = 4.16DD317 pKa = 4.26PNKK320 pKa = 9.94TFPGQCGCGVVDD332 pKa = 4.29TDD334 pKa = 4.36SDD336 pKa = 4.18GDD338 pKa = 3.93GTPDD342 pKa = 5.32CFDD345 pKa = 4.08KK346 pKa = 11.3CPNDD350 pKa = 3.66ADD352 pKa = 3.77KK353 pKa = 11.11TEE355 pKa = 4.89PGQCGCGVADD365 pKa = 4.99ADD367 pKa = 4.32SDD369 pKa = 4.29GDD371 pKa = 4.05GTPDD375 pKa = 5.26CSDD378 pKa = 3.76KK379 pKa = 11.35CPNDD383 pKa = 3.6PDD385 pKa = 3.59KK386 pKa = 11.31TEE388 pKa = 4.91PGQCGCGIADD398 pKa = 3.99TDD400 pKa = 3.85SDD402 pKa = 4.16GDD404 pKa = 4.02GVADD408 pKa = 4.17CEE410 pKa = 4.47SEE412 pKa = 4.29AVSGIPVPISPEE424 pKa = 3.69NGEE427 pKa = 4.32TVAEE431 pKa = 4.29DD432 pKa = 3.58SVTLVAQVDD441 pKa = 3.84LGSTVDD447 pKa = 4.58GPMEE451 pKa = 4.37IYY453 pKa = 8.94WWWRR457 pKa = 11.84PVDD460 pKa = 3.84KK461 pKa = 10.74KK462 pKa = 10.62CTVSSEE468 pKa = 3.98TTEE471 pKa = 4.08AYY473 pKa = 10.01RR474 pKa = 11.84GLAEE478 pKa = 4.58LRR480 pKa = 11.84ISGLRR485 pKa = 11.84DD486 pKa = 2.97GLQYY490 pKa = 11.13AWQVGVRR497 pKa = 11.84NPASGEE503 pKa = 3.8IFISDD508 pKa = 3.32EE509 pKa = 4.07VFFTVGASEE518 pKa = 4.42VEE520 pKa = 4.23VPFRR524 pKa = 11.84VDD526 pKa = 4.66AGTTLNDD533 pKa = 2.91YY534 pKa = 11.18RR535 pKa = 11.84MYY537 pKa = 10.98SFSLWPEE544 pKa = 4.04DD545 pKa = 4.45ADD547 pKa = 4.71AADD550 pKa = 4.27LLGDD554 pKa = 3.85AVGEE558 pKa = 4.03YY559 pKa = 8.24DD560 pKa = 3.72TKK562 pKa = 10.68FFKK565 pKa = 10.51IGTYY569 pKa = 10.36DD570 pKa = 3.67PEE572 pKa = 3.73QSRR575 pKa = 11.84YY576 pKa = 8.54VEE578 pKa = 4.15YY579 pKa = 10.72DD580 pKa = 2.7SGAITIVPGQAYY592 pKa = 7.88WFLARR597 pKa = 11.84NGLEE601 pKa = 3.68ASIRR605 pKa = 11.84GIKK608 pKa = 10.27VSVAEE613 pKa = 4.64NIHH616 pKa = 4.29VHH618 pKa = 5.8LRR620 pKa = 11.84YY621 pKa = 10.36DD622 pKa = 3.43EE623 pKa = 4.14TRR625 pKa = 11.84EE626 pKa = 4.15KK627 pKa = 9.22GWNMIAPPNDD637 pKa = 2.92GDD639 pKa = 4.03YY640 pKa = 10.67FRR642 pKa = 11.84KK643 pKa = 10.21DD644 pKa = 3.25IQVIAYY650 pKa = 7.03TEE652 pKa = 4.21KK653 pKa = 10.31PDD655 pKa = 3.52GSFEE659 pKa = 4.14AEE661 pKa = 4.31PGFEE665 pKa = 4.26TPVPLSALEE674 pKa = 4.82ADD676 pKa = 4.61NPWLDD681 pKa = 3.52LVLWKK686 pKa = 10.21WEE688 pKa = 3.68GGRR691 pKa = 11.84YY692 pKa = 9.43VSMTPDD698 pKa = 3.25DD699 pKa = 3.91AAAVLLRR706 pKa = 11.84NRR708 pKa = 11.84GYY710 pKa = 9.53WVNARR715 pKa = 11.84RR716 pKa = 11.84GNICLVFPGSARR728 pKa = 11.84KK729 pKa = 9.3DD730 pKa = 3.55LPEE733 pKa = 6.28SEE735 pKa = 4.72DD736 pKa = 3.55TLATMMRR743 pKa = 11.84RR744 pKa = 11.84GGEE747 pKa = 3.54WMGRR751 pKa = 11.84HH752 pKa = 5.64LTPDD756 pKa = 3.16AAVADD761 pKa = 4.23SGEE764 pKa = 4.34TPPMPIGYY772 pKa = 8.82SGSGASGTGGGVDD785 pKa = 3.08SGGGDD790 pKa = 3.35VLLTRR795 pKa = 5.11

Molecular weight: 84.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A401FSD9|A0A401FSD9_9DELT Ethanolamine ammonia-lyase OS=Desulfonema ishimotonii OX=45657 GN=DENIS_0833 PE=4 SV=1
MM1 pKa = 8.19AIRR4 pKa = 11.84KK5 pKa = 7.27IHH7 pKa = 6.69RR8 pKa = 11.84NITLWRR14 pKa = 11.84LTSDD18 pKa = 4.24FYY20 pKa = 11.68QFVRR24 pKa = 11.84SQRR27 pKa = 11.84FLTRR31 pKa = 11.84RR32 pKa = 11.84MGQPFSRR39 pKa = 11.84SRR41 pKa = 11.84KK42 pKa = 8.2FVEE45 pKa = 4.24MDD47 pKa = 2.99ITWRR51 pKa = 11.84CNLKK55 pKa = 10.64CPNCNRR61 pKa = 11.84SCTQVPSRR69 pKa = 11.84RR70 pKa = 11.84EE71 pKa = 3.63MSVGQVASFIRR82 pKa = 11.84EE83 pKa = 4.23SIAMGAQWEE92 pKa = 4.49RR93 pKa = 11.84IRR95 pKa = 11.84VLGGEE100 pKa = 4.3PTLHH104 pKa = 6.49RR105 pKa = 11.84GFFAILEE112 pKa = 4.28LLRR115 pKa = 11.84HH116 pKa = 5.51YY117 pKa = 10.34RR118 pKa = 11.84AAHH121 pKa = 5.33NPGLRR126 pKa = 11.84IVVCTNGHH134 pKa = 5.29GDD136 pKa = 3.29RR137 pKa = 11.84VNRR140 pKa = 11.84VLDD143 pKa = 4.99RR144 pKa = 11.84IPAGIAVKK152 pKa = 9.22NTFKK156 pKa = 10.49TAGPRR161 pKa = 11.84LFRR164 pKa = 11.84PFNVAPVDD172 pKa = 3.21THH174 pKa = 6.87RR175 pKa = 11.84YY176 pKa = 9.22RR177 pKa = 11.84FADD180 pKa = 3.4YY181 pKa = 11.15SGGCRR186 pKa = 11.84IISEE190 pKa = 4.4CGIGVTPLGCYY201 pKa = 9.14PCAIAGGIDD210 pKa = 3.52RR211 pKa = 11.84IFGFGLGRR219 pKa = 11.84KK220 pKa = 8.96KK221 pKa = 10.87LPLPDD226 pKa = 5.09DD227 pKa = 3.75EE228 pKa = 5.21MRR230 pKa = 11.84DD231 pKa = 3.5HH232 pKa = 7.15LKK234 pKa = 10.25IFCRR238 pKa = 11.84LCGHH242 pKa = 7.11FGFAWPTRR250 pKa = 11.84KK251 pKa = 9.71PEE253 pKa = 4.36ISKK256 pKa = 8.3TWEE259 pKa = 3.46NAYY262 pKa = 10.47RR263 pKa = 11.84NCRR266 pKa = 11.84AGNSSEE272 pKa = 4.0RR273 pKa = 3.74

Molecular weight: 31.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5012

0

5012

1708607

29

5505

340.9

37.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.708 ± 0.043

1.293 ± 0.015

5.892 ± 0.046

6.604 ± 0.031

4.347 ± 0.025

7.81 ± 0.036

1.993 ± 0.016

6.572 ± 0.032

5.113 ± 0.038

9.336 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.661 ± 0.018

3.473 ± 0.026

4.629 ± 0.026

2.988 ± 0.019

6.657 ± 0.041

5.733 ± 0.029

5.339 ± 0.04

6.645 ± 0.031

1.168 ± 0.014

3.038 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski