Actinoallomurus bryophytorum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinoallomurus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8518 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A543CFZ8|A0A543CFZ8_9ACTN Thiamine kinase-like enzyme OS=Actinoallomurus bryophytorum OX=1490222 GN=FB559_1565 PE=4 SV=1
MM1 pKa = 7.53LKK3 pKa = 10.65ALLGQLAEE11 pKa = 4.79LLDD14 pKa = 4.48PDD16 pKa = 4.89ADD18 pKa = 3.75LHH20 pKa = 8.09AGDD23 pKa = 5.49DD24 pKa = 3.75SHH26 pKa = 8.74ALDD29 pKa = 3.98EE30 pKa = 4.85YY31 pKa = 11.24YY32 pKa = 11.05DD33 pKa = 3.84NEE35 pKa = 4.16

Molecular weight: 3.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A543CQW9|A0A543CQW9_9ACTN PS-10 peptidase S37 OS=Actinoallomurus bryophytorum OX=1490222 GN=FB559_5177 PE=4 SV=1
MM1 pKa = 7.71LAPPLRR7 pKa = 11.84RR8 pKa = 11.84SAAPPLRR15 pKa = 11.84RR16 pKa = 11.84SAAPPLRR23 pKa = 11.84RR24 pKa = 11.84SAAPPLRR31 pKa = 11.84RR32 pKa = 11.84SAAPPLRR39 pKa = 11.84RR40 pKa = 11.84SAAPPLRR47 pKa = 11.84RR48 pKa = 11.84SAAPPLRR55 pKa = 11.84RR56 pKa = 11.84SAAPPLRR63 pKa = 11.84RR64 pKa = 11.84SAAPPLRR71 pKa = 11.84RR72 pKa = 11.84SAAPPLRR79 pKa = 11.84RR80 pKa = 11.84SAAPPLRR87 pKa = 11.84RR88 pKa = 11.84SAAPPLRR95 pKa = 11.84RR96 pKa = 11.84SAAPPLRR103 pKa = 11.84RR104 pKa = 11.84SAAPPLRR111 pKa = 11.84RR112 pKa = 11.84SAAPPLRR119 pKa = 11.84RR120 pKa = 11.84SAAPPLRR127 pKa = 11.84RR128 pKa = 11.84SAAPPLRR135 pKa = 11.84RR136 pKa = 11.84SAAPPLRR143 pKa = 11.84RR144 pKa = 11.84SAAPPLRR151 pKa = 11.84RR152 pKa = 11.84SAAPPLRR159 pKa = 11.84RR160 pKa = 11.84SAAPPLRR167 pKa = 11.84RR168 pKa = 11.84SAAPPLRR175 pKa = 11.84RR176 pKa = 11.84SAAPPLRR183 pKa = 11.84RR184 pKa = 11.84SAAPPLRR191 pKa = 11.84RR192 pKa = 11.84SAAPPLRR199 pKa = 11.84RR200 pKa = 11.84SAAPPLRR207 pKa = 11.84RR208 pKa = 11.84SAAPPLRR215 pKa = 11.84RR216 pKa = 11.84SAAPPLRR223 pKa = 11.84RR224 pKa = 11.84SAAPPLRR231 pKa = 11.84RR232 pKa = 11.84SAAPPLRR239 pKa = 11.84RR240 pKa = 11.84SAAPPLRR247 pKa = 11.84RR248 pKa = 11.84SAAPPLRR255 pKa = 11.84RR256 pKa = 11.84SAAPPLRR263 pKa = 11.84RR264 pKa = 11.84SAAPPLRR271 pKa = 11.84RR272 pKa = 11.84SAAPPLRR279 pKa = 11.84RR280 pKa = 11.84PADD283 pKa = 3.67PPVLCTSRR291 pKa = 11.84CLAPRR296 pKa = 11.84RR297 pKa = 11.84IQPRR301 pKa = 11.84QGRR304 pKa = 11.84KK305 pKa = 8.4SAVRR309 pKa = 11.84APPAAAPNRR318 pKa = 11.84SHH320 pKa = 6.8GVIHH324 pKa = 7.17RR325 pKa = 4.06

Molecular weight: 34.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8518

0

8518

2754208

29

15440

323.3

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.107 ± 0.034

0.777 ± 0.01

5.977 ± 0.021

5.48 ± 0.029

2.8 ± 0.015

9.535 ± 0.027

2.304 ± 0.012

3.722 ± 0.017

1.986 ± 0.019

10.205 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.86 ± 0.01

1.859 ± 0.016

6.024 ± 0.028

2.653 ± 0.015

8.268 ± 0.032

5.223 ± 0.025

6.093 ± 0.025

8.485 ± 0.026

1.513 ± 0.011

2.131 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski