Usitatibacter palustris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Usitatibacteraceae; Usitatibacter

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3756 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M4H3B2|A0A6M4H3B2_9PROT Methyl-accepting transducer domain-containing protein OS=Usitatibacter palustris OX=2732487 GN=DSM104440_00808 PE=4 SV=1
MM1 pKa = 7.62NDD3 pKa = 3.05MTTALPDD10 pKa = 3.89PLLFSEE16 pKa = 4.67SAANKK21 pKa = 9.18VKK23 pKa = 10.78ALIEE27 pKa = 4.12EE28 pKa = 4.45EE29 pKa = 4.43GNNDD33 pKa = 2.75LKK35 pKa = 11.35LRR37 pKa = 11.84VFVTGGGCSGLQYY50 pKa = 11.21GFTFDD55 pKa = 4.62EE56 pKa = 4.69MTNEE60 pKa = 5.1DD61 pKa = 4.13DD62 pKa = 5.31TVMQKK67 pKa = 10.76NGVSLLIDD75 pKa = 3.63PMSYY79 pKa = 10.24QYY81 pKa = 11.93LMGAEE86 pKa = 3.82IDD88 pKa = 3.83YY89 pKa = 10.8TEE91 pKa = 4.44GLEE94 pKa = 4.23GAQFVIKK101 pKa = 10.56NPNAVSTCGCGSSFTVV117 pKa = 3.3

Molecular weight: 12.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M4HBZ2|A0A6M4HBZ2_9PROT Uncharacterized protein OS=Usitatibacter palustris OX=2732487 GN=DSM104440_02952 PE=4 SV=1
MM1 pKa = 7.91PKK3 pKa = 9.63MKK5 pKa = 9.69TKK7 pKa = 10.59SGAAKK12 pKa = 9.64RR13 pKa = 11.84FRR15 pKa = 11.84VRR17 pKa = 11.84GSGSVKK23 pKa = 9.19RR24 pKa = 11.84TMAGKK29 pKa = 9.88RR30 pKa = 11.84HH31 pKa = 6.26ILTKK35 pKa = 10.02RR36 pKa = 11.84TTKK39 pKa = 10.2SKK41 pKa = 9.81RR42 pKa = 11.84QLRR45 pKa = 11.84GTTGVHH51 pKa = 5.29KK52 pKa = 9.76TNAAHH57 pKa = 5.98VKK59 pKa = 10.74AMLPYY64 pKa = 10.54AA65 pKa = 4.38

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3756

0

3756

1238078

29

5825

329.6

35.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.569 ± 0.052

0.906 ± 0.014

5.173 ± 0.029

5.604 ± 0.053

3.832 ± 0.025

8.417 ± 0.046

2.045 ± 0.024

4.769 ± 0.027

4.011 ± 0.039

9.774 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.354 ± 0.024

2.8 ± 0.038

5.299 ± 0.033

2.974 ± 0.02

6.862 ± 0.056

5.299 ± 0.035

5.565 ± 0.075

7.96 ± 0.034

1.393 ± 0.018

2.392 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski