Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936) (Fusarium vascular wilt of tomato)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; Fusarium oxysporum species complex; Fusarium oxysporum; Fusarium oxysporum f. sp. lycopersici

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16646 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D2XVT7|A0A0D2XVT7_FUSO4 Uncharacterized protein OS=Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936) OX=426428 GN=FOXG_08099 PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 10.48SSFLTIFGLAAAAVAQSSDD21 pKa = 4.31DD22 pKa = 3.79LPQCGRR28 pKa = 11.84TCAGNMVSAEE38 pKa = 3.88KK39 pKa = 10.54SQEE42 pKa = 4.08LGCDD46 pKa = 3.26AGDD49 pKa = 3.95IGCLCTNQNFIYY61 pKa = 10.55GLRR64 pKa = 11.84DD65 pKa = 3.29CSAAICNGEE74 pKa = 3.94QAAQVLNYY82 pKa = 10.16GLEE85 pKa = 3.7ICRR88 pKa = 11.84RR89 pKa = 11.84AGVQITTGASGEE101 pKa = 4.25VSATATGSGAVRR113 pKa = 11.84TVLSTLTSGDD123 pKa = 3.52STITSALSTISGTATGASDD142 pKa = 3.87DD143 pKa = 4.25VSVSTYY149 pKa = 10.66TSVLTNSEE157 pKa = 3.75GDD159 pKa = 3.34EE160 pKa = 4.06FTTTGKK166 pKa = 10.59AILGGAVVTTFTSGGSTIVSTITSGSEE193 pKa = 4.06TEE195 pKa = 4.1TSGAEE200 pKa = 4.08SAEE203 pKa = 4.19VTTFTSDD210 pKa = 2.47GTEE213 pKa = 3.58IVRR216 pKa = 11.84TLVTEE221 pKa = 4.3TASTDD226 pKa = 3.35SAASAEE232 pKa = 4.45VTTFTTDD239 pKa = 2.54GTEE242 pKa = 4.14VVRR245 pKa = 11.84TLTTVTSGSQSEE257 pKa = 4.77SVSEE261 pKa = 4.31TVTDD265 pKa = 3.84ASTATEE271 pKa = 4.36GATSATGTDD280 pKa = 3.55ASATTTGTDD289 pKa = 3.02NAAAAQMTGAPAGVIAAAGIAMLLLL314 pKa = 4.24

Molecular weight: 30.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D2XNR3|A0A0D2XNR3_FUSO4 Uncharacterized protein OS=Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936) OX=426428 PE=4 SV=1
MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.63RR8 pKa = 11.84HH9 pKa = 4.04TAPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APAHH26 pKa = 4.84SHH28 pKa = 5.33RR29 pKa = 11.84HH30 pKa = 4.03TTTTTTTTTKK40 pKa = 9.5PRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84GMFGGGAGRR53 pKa = 11.84RR54 pKa = 11.84THH56 pKa = 6.46ATTTAPVHH64 pKa = 4.87HH65 pKa = 6.63HH66 pKa = 5.14QRR68 pKa = 11.84RR69 pKa = 11.84PSMKK73 pKa = 10.02DD74 pKa = 2.95KK75 pKa = 11.41VSGALLKK82 pKa = 11.04LKK84 pKa = 10.68GSLTRR89 pKa = 11.84RR90 pKa = 11.84PGVKK94 pKa = 9.89AAGTRR99 pKa = 11.84RR100 pKa = 11.84MRR102 pKa = 11.84GTDD105 pKa = 3.02GRR107 pKa = 11.84GARR110 pKa = 11.84HH111 pKa = 5.87HH112 pKa = 7.23RR113 pKa = 11.84YY114 pKa = 9.44

Molecular weight: 12.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16636

10

16646

6886139

13

7531

413.7

45.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.416 ± 0.015

1.274 ± 0.007

5.86 ± 0.017

6.256 ± 0.021

3.786 ± 0.011

6.873 ± 0.019

2.36 ± 0.008

5.121 ± 0.013

5.105 ± 0.016

8.777 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.266 ± 0.008

3.788 ± 0.011

5.811 ± 0.02

3.998 ± 0.015

5.868 ± 0.017

7.95 ± 0.019

5.945 ± 0.019

6.154 ± 0.014

1.56 ± 0.007

2.831 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski