Paraburkholderia sp. DHOC27

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia; unclassified Paraburkholderia

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6264 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A372K7M5|A0A372K7M5_9BURK Uncharacterized protein OS=Paraburkholderia sp. DHOC27 OX=2303330 GN=D0B32_00095 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 9.82IGSISGIAQLSLSASLAFSLAACGGGGGSSASTSNQPVTAAATSMTGTVAIGTALTGATVTVTDD66 pKa = 4.37ANGKK70 pKa = 6.06TVSAASGANGAYY82 pKa = 9.73SVSLTGLSAPLLITAADD99 pKa = 3.86PSGVSGTLYY108 pKa = 10.75SVVASTNTTSGAPVTANATPLTTAVAALMTQSGNPADD145 pKa = 3.93LAGNASAITSSAITAAEE162 pKa = 4.18TTLDD166 pKa = 3.66SAIAPILSANSVPASFDD183 pKa = 4.17PIGTTFTPNQTGADD197 pKa = 3.51AVIDD201 pKa = 4.13SVAVTPSASGSGFQITSLANPNTAIQLNSSTSVSTALVAPAQAANYY247 pKa = 8.18LASLQASLSACASDD261 pKa = 3.99VQGGATDD268 pKa = 3.88TSDD271 pKa = 4.11SNCMSAIDD279 pKa = 5.05ANYY282 pKa = 9.93LNNGVGTGVAGFAKK296 pKa = 10.02RR297 pKa = 11.84HH298 pKa = 4.92SLFTKK303 pKa = 9.03GTVLTGIKK311 pKa = 9.41TIAFVPAGTLAGISNPAALVYY332 pKa = 11.0LLMIDD337 pKa = 4.14PDD339 pKa = 3.92GTPDD343 pKa = 3.21FGMDD347 pKa = 3.71YY348 pKa = 10.74VQQLPNGKK356 pKa = 8.37WDD358 pKa = 3.71VIGNQLQDD366 pKa = 3.18GTYY369 pKa = 9.19IASFLGRR376 pKa = 11.84VQYY379 pKa = 10.48TDD381 pKa = 3.32SADD384 pKa = 3.27AGNAHH389 pKa = 6.4YY390 pKa = 10.43EE391 pKa = 4.19SGLDD395 pKa = 3.47VQIPSSVKK403 pKa = 9.8VNGTATSVGSAVVTGPGLPTNGLWFQSAANGTGAGYY439 pKa = 10.93LDD441 pKa = 4.62IPTGTLTAPLTSTGNRR457 pKa = 11.84VNGGMSTTYY466 pKa = 9.45KK467 pKa = 9.53WAWAPVSGGTTSFSPSGLPEE487 pKa = 4.08YY488 pKa = 10.56ASSSQDD494 pKa = 2.86VSAITNFGIYY504 pKa = 9.18TVTLYY509 pKa = 8.99DD510 pKa = 3.43TTGTEE515 pKa = 3.83IGSEE519 pKa = 4.03QIQNIARR526 pKa = 11.84NYY528 pKa = 8.75AAAAGSTVTWQTLGNDD544 pKa = 3.97VIANYY549 pKa = 7.53LTPGGSGTQSAPGTSTALDD568 pKa = 3.44WATPAGSFYY577 pKa = 10.54PNFWASINSLGVAQASIPATTYY599 pKa = 11.1DD600 pKa = 3.26ATVWGASTGNTPSPLTFSTSFTDD623 pKa = 3.49VLTSTATATAEE634 pKa = 4.03QAVQVQLGWQADD646 pKa = 3.58GEE648 pKa = 5.02YY649 pKa = 10.85YY650 pKa = 10.97VNTWQYY656 pKa = 11.4GNPP659 pKa = 3.6

Molecular weight: 65.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A372KAU9|A0A372KAU9_9BURK MFS transporter OS=Paraburkholderia sp. DHOC27 OX=2303330 GN=D0B32_02345 PE=4 SV=1
MM1 pKa = 7.24AAIVEE6 pKa = 4.48FRR8 pKa = 11.84APGRR12 pKa = 11.84RR13 pKa = 11.84GRR15 pKa = 11.84RR16 pKa = 11.84VVGHH20 pKa = 6.7AVPGVGLRR28 pKa = 11.84VRR30 pKa = 11.84RR31 pKa = 11.84TARR34 pKa = 11.84QGVGRR39 pKa = 11.84MLRR42 pKa = 11.84QSVRR46 pKa = 11.84RR47 pKa = 11.84MLRR50 pKa = 11.84RR51 pKa = 11.84VLGQMPRR58 pKa = 11.84QGLRR62 pKa = 11.84QRR64 pKa = 11.84LSHH67 pKa = 7.07PAPQGLFPP75 pKa = 5.6

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6264

0

6264

2023864

19

3196

323.1

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.663 ± 0.043

0.909 ± 0.009

5.358 ± 0.022

5.162 ± 0.033

3.701 ± 0.024

8.08 ± 0.034

2.367 ± 0.016

4.703 ± 0.02

3.035 ± 0.022

10.293 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.354 ± 0.015

2.877 ± 0.025

5.083 ± 0.024

3.757 ± 0.023

6.665 ± 0.036

5.812 ± 0.027

5.595 ± 0.03

7.792 ± 0.027

1.372 ± 0.013

2.423 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski