Amino acid dipepetide frequency for Paraburkholderia sp. DHOC27

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.593AlaAla: 17.593 ± 0.126
1.24AlaCys: 1.24 ± 0.027
6.347AlaAsp: 6.347 ± 0.057
6.146AlaGlu: 6.146 ± 0.065
4.356AlaPhe: 4.356 ± 0.052
10.199AlaGly: 10.199 ± 0.088
2.876AlaHis: 2.876 ± 0.042
5.637AlaIle: 5.637 ± 0.059
3.593AlaLys: 3.593 ± 0.052
14.185AlaLeu: 14.185 ± 0.104
3.189AlaMet: 3.189 ± 0.041
3.489AlaAsn: 3.489 ± 0.045
5.913AlaPro: 5.913 ± 0.068
5.496AlaGln: 5.496 ± 0.059
8.613AlaArg: 8.613 ± 0.08
7.764AlaSer: 7.764 ± 0.074
6.701AlaThr: 6.701 ± 0.077
8.926AlaVal: 8.926 ± 0.088
1.72AlaTrp: 1.72 ± 0.031
2.647AlaTyr: 2.647 ± 0.035
0.0AlaXaa: 0.0 ± 0.0
Cys
1.188CysAla: 1.188 ± 0.026
0.113CysCys: 0.113 ± 0.009
0.512CysAsp: 0.512 ± 0.016
0.496CysGlu: 0.496 ± 0.015
0.341CysPhe: 0.341 ± 0.013
0.969CysGly: 0.969 ± 0.025
0.265CysHis: 0.265 ± 0.011
0.396CysIle: 0.396 ± 0.013
0.21CysLys: 0.21 ± 0.01
0.847CysLeu: 0.847 ± 0.022
0.198CysMet: 0.198 ± 0.011
0.247CysAsn: 0.247 ± 0.011
0.413CysPro: 0.413 ± 0.016
0.22CysGln: 0.22 ± 0.011
0.579CysArg: 0.579 ± 0.017
0.496CysSer: 0.496 ± 0.017
0.468CysThr: 0.468 ± 0.017
0.789CysVal: 0.789 ± 0.023
0.121CysTrp: 0.121 ± 0.008
0.225CysTyr: 0.225 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.411AspAla: 7.411 ± 0.07
0.461AspCys: 0.461 ± 0.016
3.121AspAsp: 3.121 ± 0.052
3.376AspGlu: 3.376 ± 0.059
1.94AspPhe: 1.94 ± 0.036
4.475AspGly: 4.475 ± 0.049
1.215AspHis: 1.215 ± 0.027
2.499AspIle: 2.499 ± 0.039
1.526AspLys: 1.526 ± 0.033
5.213AspLeu: 5.213 ± 0.058
1.162AspMet: 1.162 ± 0.024
1.367AspAsn: 1.367 ± 0.031
2.863AspPro: 2.863 ± 0.043
1.692AspGln: 1.692 ± 0.03
3.185AspArg: 3.185 ± 0.041
2.595AspSer: 2.595 ± 0.036
2.818AspThr: 2.818 ± 0.038
4.223AspVal: 4.223 ± 0.043
0.923AspTrp: 0.923 ± 0.021
1.515AspTyr: 1.515 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
6.642GluAla: 6.642 ± 0.067
0.395GluCys: 0.395 ± 0.015
2.146GluAsp: 2.146 ± 0.034
2.437GluGlu: 2.437 ± 0.046
1.794GluPhe: 1.794 ± 0.03
3.394GluGly: 3.394 ± 0.043
1.467GluHis: 1.467 ± 0.028
2.768GluIle: 2.768 ± 0.042
1.779GluLys: 1.779 ± 0.037
5.365GluLeu: 5.365 ± 0.068
1.227GluMet: 1.227 ± 0.023
1.381GluAsn: 1.381 ± 0.029
2.478GluPro: 2.478 ± 0.039
2.426GluGln: 2.426 ± 0.04
4.72GluArg: 4.72 ± 0.066
2.648GluSer: 2.648 ± 0.037
2.918GluThr: 2.918 ± 0.035
3.752GluVal: 3.752 ± 0.049
0.719GluTrp: 0.719 ± 0.018
1.159GluTyr: 1.159 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
4.733PheAla: 4.733 ± 0.052
0.427PheCys: 0.427 ± 0.015
2.689PheAsp: 2.689 ± 0.042
2.061PheGlu: 2.061 ± 0.031
1.441PhePhe: 1.441 ± 0.035
3.655PheGly: 3.655 ± 0.051
0.766PheHis: 0.766 ± 0.021
1.69PheIle: 1.69 ± 0.028
1.027PheLys: 1.027 ± 0.025
3.019PheLeu: 3.019 ± 0.043
0.804PheMet: 0.804 ± 0.021
1.167PheAsn: 1.167 ± 0.025
1.574PhePro: 1.574 ± 0.029
1.037PheGln: 1.037 ± 0.024
1.975PheArg: 1.975 ± 0.033
2.331PheSer: 2.331 ± 0.032
2.002PheThr: 2.002 ± 0.032
3.111PheVal: 3.111 ± 0.047
0.51PheTrp: 0.51 ± 0.016
0.995PheTyr: 0.995 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
9.345GlyAla: 9.345 ± 0.081
0.832GlyCys: 0.832 ± 0.02
3.837GlyAsp: 3.837 ± 0.042
4.223GlyGlu: 4.223 ± 0.051
3.402GlyPhe: 3.402 ± 0.051
6.603GlyGly: 6.603 ± 0.086
1.972GlyHis: 1.972 ± 0.031
4.187GlyIle: 4.187 ± 0.049
3.198GlyLys: 3.198 ± 0.047
7.912GlyLeu: 7.912 ± 0.076
2.176GlyMet: 2.176 ± 0.035
2.428GlyAsn: 2.428 ± 0.053
2.915GlyPro: 2.915 ± 0.041
2.852GlyGln: 2.852 ± 0.04
4.883GlyArg: 4.883 ± 0.054
4.794GlySer: 4.794 ± 0.065
4.81GlyThr: 4.81 ± 0.079
6.711GlyVal: 6.711 ± 0.07
1.344GlyTrp: 1.344 ± 0.03
2.372GlyTyr: 2.372 ± 0.032
0.0GlyXaa: 0.0 ± 0.0
His
3.248HisAla: 3.248 ± 0.046
0.257HisCys: 0.257 ± 0.011
1.4HisAsp: 1.4 ± 0.029
1.253HisGlu: 1.253 ± 0.027
0.983HisPhe: 0.983 ± 0.019
2.179HisGly: 2.179 ± 0.039
0.663HisHis: 0.663 ± 0.022
0.947HisIle: 0.947 ± 0.02
0.522HisLys: 0.522 ± 0.018
2.28HisLeu: 2.28 ± 0.035
0.523HisMet: 0.523 ± 0.015
0.562HisAsn: 0.562 ± 0.015
1.472HisPro: 1.472 ± 0.028
0.71HisGln: 0.71 ± 0.019
1.495HisArg: 1.495 ± 0.03
1.102HisSer: 1.102 ± 0.025
1.184HisThr: 1.184 ± 0.027
1.797HisVal: 1.797 ± 0.033
0.403HisTrp: 0.403 ± 0.015
0.692HisTyr: 0.692 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
6.738IleAla: 6.738 ± 0.061
0.466IleCys: 0.466 ± 0.016
3.29IleAsp: 3.29 ± 0.038
3.277IleGlu: 3.277 ± 0.041
1.53IlePhe: 1.53 ± 0.03
4.605IleGly: 4.605 ± 0.051
0.945IleHis: 0.945 ± 0.023
1.734IleIle: 1.734 ± 0.03
1.424IleLys: 1.424 ± 0.029
3.541IleLeu: 3.541 ± 0.046
0.791IleMet: 0.791 ± 0.022
1.491IleAsn: 1.491 ± 0.03
2.15IlePro: 2.15 ± 0.032
1.327IleGln: 1.327 ± 0.029
2.719IleArg: 2.719 ± 0.04
2.564IleSer: 2.564 ± 0.043
2.563IleThr: 2.563 ± 0.043
4.309IleVal: 4.309 ± 0.045
0.54IleTrp: 0.54 ± 0.017
1.03IleTyr: 1.03 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
3.539LysAla: 3.539 ± 0.054
0.171LysCys: 0.171 ± 0.009
1.482LysAsp: 1.482 ± 0.031
1.388LysGlu: 1.388 ± 0.03
0.884LysPhe: 0.884 ± 0.022
2.081LysGly: 2.081 ± 0.039
0.695LysHis: 0.695 ± 0.019
1.564LysIle: 1.564 ± 0.029
1.185LysLys: 1.185 ± 0.036
3.259LysLeu: 3.259 ± 0.044
0.793LysMet: 0.793 ± 0.023
0.864LysAsn: 0.864 ± 0.024
1.93LysPro: 1.93 ± 0.035
1.327LysGln: 1.327 ± 0.027
2.227LysArg: 2.227 ± 0.034
1.698LysSer: 1.698 ± 0.029
1.839LysThr: 1.839 ± 0.034
2.387LysVal: 2.387 ± 0.042
0.361LysTrp: 0.361 ± 0.015
0.674LysTyr: 0.674 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
13.804LeuAla: 13.804 ± 0.103
1.033LeuCys: 1.033 ± 0.026
5.959LeuAsp: 5.959 ± 0.061
5.047LeuGlu: 5.047 ± 0.066
3.652LeuPhe: 3.652 ± 0.05
8.104LeuGly: 8.104 ± 0.065
2.329LeuHis: 2.329 ± 0.037
4.888LeuIle: 4.888 ± 0.057
3.301LeuLys: 3.301 ± 0.043
10.238LeuLeu: 10.238 ± 0.097
2.281LeuMet: 2.281 ± 0.033
2.931LeuAsn: 2.931 ± 0.04
5.658LeuPro: 5.658 ± 0.063
3.625LeuGln: 3.625 ± 0.033
6.874LeuArg: 6.874 ± 0.07
6.453LeuSer: 6.453 ± 0.063
5.899LeuThr: 5.899 ± 0.062
7.5LeuVal: 7.5 ± 0.074
1.133LeuTrp: 1.133 ± 0.03
2.216LeuTyr: 2.216 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
2.526MetAla: 2.526 ± 0.038
0.179MetCys: 0.179 ± 0.009
0.964MetAsp: 0.964 ± 0.025
0.912MetGlu: 0.912 ± 0.023
0.78MetPhe: 0.78 ± 0.022
1.573MetGly: 1.573 ± 0.029
0.549MetHis: 0.549 ± 0.017
1.126MetIle: 1.126 ± 0.025
0.995MetLys: 0.995 ± 0.024
2.678MetLeu: 2.678 ± 0.04
0.614MetMet: 0.614 ± 0.019
0.934MetAsn: 0.934 ± 0.022
1.49MetPro: 1.49 ± 0.028
1.018MetGln: 1.018 ± 0.023
1.724MetArg: 1.724 ± 0.029
1.726MetSer: 1.726 ± 0.028
1.596MetThr: 1.596 ± 0.027
1.523MetVal: 1.523 ± 0.031
0.212MetTrp: 0.212 ± 0.011
0.418MetTyr: 0.418 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.724AsnAla: 3.724 ± 0.049
0.253AsnCys: 0.253 ± 0.012
1.525AsnAsp: 1.525 ± 0.028
1.419AsnGlu: 1.419 ± 0.025
1.053AsnPhe: 1.053 ± 0.024
2.705AsnGly: 2.705 ± 0.048
0.581AsnHis: 0.581 ± 0.018
1.206AsnIle: 1.206 ± 0.026
0.705AsnLys: 0.705 ± 0.021
2.921AsnLeu: 2.921 ± 0.042
0.575AsnMet: 0.575 ± 0.016
0.913AsnAsn: 0.913 ± 0.027
1.903AsnPro: 1.903 ± 0.033
0.993AsnGln: 0.993 ± 0.024
1.781AsnArg: 1.781 ± 0.029
1.426AsnSer: 1.426 ± 0.033
1.549AsnThr: 1.549 ± 0.033
2.312AsnVal: 2.312 ± 0.037
0.432AsnTrp: 0.432 ± 0.014
0.792AsnTyr: 0.792 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
6.558ProAla: 6.558 ± 0.069
0.344ProCys: 0.344 ± 0.013
3.315ProAsp: 3.315 ± 0.042
3.008ProGlu: 3.008 ± 0.044
1.942ProPhe: 1.942 ± 0.033
4.058ProGly: 4.058 ± 0.047
1.315ProHis: 1.315 ± 0.028
1.986ProIle: 1.986 ± 0.034
1.374ProLys: 1.374 ± 0.028
5.267ProLeu: 5.267 ± 0.054
1.063ProMet: 1.063 ± 0.023
1.499ProAsn: 1.499 ± 0.029
2.5ProPro: 2.5 ± 0.054
1.971ProGln: 1.971 ± 0.036
2.823ProArg: 2.823 ± 0.048
2.829ProSer: 2.829 ± 0.038
2.675ProThr: 2.675 ± 0.048
4.428ProVal: 4.428 ± 0.049
0.639ProTrp: 0.639 ± 0.02
1.235ProTyr: 1.235 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
4.557GlnAla: 4.557 ± 0.058
0.286GlnCys: 0.286 ± 0.011
1.469GlnAsp: 1.469 ± 0.028
1.383GlnGlu: 1.383 ± 0.031
1.334GlnPhe: 1.334 ± 0.024
2.588GlnGly: 2.588 ± 0.033
1.029GlnHis: 1.029 ± 0.023
2.068GlnIle: 2.068 ± 0.033
1.126GlnLys: 1.126 ± 0.027
3.907GlnLeu: 3.907 ± 0.049
1.037GlnMet: 1.037 ± 0.023
0.996GlnAsn: 0.996 ± 0.022
2.141GlnPro: 2.141 ± 0.038
2.072GlnGln: 2.072 ± 0.045
3.061GlnArg: 3.061 ± 0.039
2.205GlnSer: 2.205 ± 0.038
2.151GlnThr: 2.151 ± 0.035
2.606GlnVal: 2.606 ± 0.033
0.573GlnTrp: 0.573 ± 0.019
0.978GlnTyr: 0.978 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
7.847ArgAla: 7.847 ± 0.078
0.551ArgCys: 0.551 ± 0.018
3.557ArgAsp: 3.557 ± 0.044
4.094ArgGlu: 4.094 ± 0.049
2.83ArgPhe: 2.83 ± 0.041
4.439ArgGly: 4.439 ± 0.056
1.801ArgHis: 1.801 ± 0.03
3.444ArgIle: 3.444 ± 0.044
1.976ArgLys: 1.976 ± 0.032
7.018ArgLeu: 7.018 ± 0.066
1.77ArgMet: 1.77 ± 0.031
1.884ArgAsn: 1.884 ± 0.03
2.946ArgPro: 2.946 ± 0.043
2.494ArgGln: 2.494 ± 0.038
4.948ArgArg: 4.948 ± 0.065
3.573ArgSer: 3.573 ± 0.045
3.344ArgThr: 3.344 ± 0.041
5.165ArgVal: 5.165 ± 0.06
1.005ArgTrp: 1.005 ± 0.023
1.965ArgTyr: 1.965 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
7.191SerAla: 7.191 ± 0.078
0.442SerCys: 0.442 ± 0.017
2.852SerAsp: 2.852 ± 0.041
2.762SerGlu: 2.762 ± 0.036
2.256SerPhe: 2.256 ± 0.037
5.559SerGly: 5.559 ± 0.067
1.297SerHis: 1.297 ± 0.028
2.823SerIle: 2.823 ± 0.037
1.683SerLys: 1.683 ± 0.029
5.941SerLeu: 5.941 ± 0.054
1.453SerMet: 1.453 ± 0.026
1.785SerAsn: 1.785 ± 0.036
2.873SerPro: 2.873 ± 0.041
1.947SerGln: 1.947 ± 0.03
3.55SerArg: 3.55 ± 0.045
3.652SerSer: 3.652 ± 0.057
3.42SerThr: 3.42 ± 0.048
4.558SerVal: 4.558 ± 0.053
0.731SerTrp: 0.731 ± 0.018
1.347SerTyr: 1.347 ± 0.029
0.0SerXaa: 0.0 ± 0.0
Thr
6.394ThrAla: 6.394 ± 0.087
0.43ThrCys: 0.43 ± 0.015
2.725ThrAsp: 2.725 ± 0.04
2.377ThrGlu: 2.377 ± 0.035
2.053ThrPhe: 2.053 ± 0.037
4.922ThrGly: 4.922 ± 0.072
1.33ThrHis: 1.33 ± 0.028
2.624ThrIle: 2.624 ± 0.045
1.256ThrLys: 1.256 ± 0.027
6.782ThrLeu: 6.782 ± 0.081
1.15ThrMet: 1.15 ± 0.024
1.434ThrAsn: 1.434 ± 0.03
3.709ThrPro: 3.709 ± 0.052
2.165ThrGln: 2.165 ± 0.034
3.573ThrArg: 3.573 ± 0.048
3.236ThrSer: 3.236 ± 0.052
3.191ThrThr: 3.191 ± 0.061
4.59ThrVal: 4.59 ± 0.051
0.736ThrTrp: 0.736 ± 0.02
1.268ThrTyr: 1.268 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
9.593ValAla: 9.593 ± 0.078
0.794ValCys: 0.794 ± 0.017
4.327ValAsp: 4.327 ± 0.046
4.218ValGlu: 4.218 ± 0.048
2.853ValPhe: 2.853 ± 0.04
5.676ValGly: 5.676 ± 0.062
1.605ValHis: 1.605 ± 0.03
3.888ValIle: 3.888 ± 0.04
2.441ValLys: 2.441 ± 0.04
8.077ValLeu: 8.077 ± 0.071
1.927ValMet: 1.927 ± 0.031
2.279ValAsn: 2.279 ± 0.035
3.98ValPro: 3.98 ± 0.053
2.563ValGln: 2.563 ± 0.034
4.91ValArg: 4.91 ± 0.052
4.896ValSer: 4.896 ± 0.052
4.786ValThr: 4.786 ± 0.059
6.405ValVal: 6.405 ± 0.068
0.985ValTrp: 0.985 ± 0.022
1.718ValTyr: 1.718 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.182TrpAla: 1.182 ± 0.029
0.157TrpCys: 0.157 ± 0.009
0.626TrpAsp: 0.626 ± 0.018
0.498TrpGlu: 0.498 ± 0.017
0.575TrpPhe: 0.575 ± 0.016
0.855TrpGly: 0.855 ± 0.02
0.423TrpHis: 0.423 ± 0.013
0.696TrpIle: 0.696 ± 0.021
0.415TrpLys: 0.415 ± 0.014
1.886TrpLeu: 1.886 ± 0.035
0.368TrpMet: 0.368 ± 0.014
0.472TrpAsn: 0.472 ± 0.016
0.652TrpPro: 0.652 ± 0.02
0.711TrpGln: 0.711 ± 0.021
1.164TrpArg: 1.164 ± 0.026
0.829TrpSer: 0.829 ± 0.022
0.716TrpThr: 0.716 ± 0.018
0.948TrpVal: 0.948 ± 0.023
0.234TrpTrp: 0.234 ± 0.011
0.316TrpTyr: 0.316 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.816TyrAla: 2.816 ± 0.041
0.261TyrCys: 0.261 ± 0.011
1.434TyrAsp: 1.434 ± 0.034
1.242TyrGlu: 1.242 ± 0.027
1.016TyrPhe: 1.016 ± 0.021
2.156TyrGly: 2.156 ± 0.038
0.549TyrHis: 0.549 ± 0.016
0.861TyrIle: 0.861 ± 0.019
0.616TyrLys: 0.616 ± 0.018
2.593TyrLeu: 2.593 ± 0.04
0.439TyrMet: 0.439 ± 0.014
0.645TyrAsn: 0.645 ± 0.018
1.268TyrPro: 1.268 ± 0.027
0.92TyrGln: 0.92 ± 0.022
1.845TyrArg: 1.845 ± 0.03
1.304TyrSer: 1.304 ± 0.027
1.316TyrThr: 1.316 ± 0.03
1.884TyrVal: 1.884 ± 0.032
0.399TyrTrp: 0.399 ± 0.015
0.663TyrTyr: 0.663 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6264 proteins (2023864 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski