Brevibacillus phage Jimmer1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Jimmervirus; unclassified Jimmervirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5MBT5|S5MBT5_9CAUD Uncharacterized protein OS=Brevibacillus phage Jimmer1 OX=1296659 GN=JIMMER1_96 PE=4 SV=1
MM1 pKa = 7.6SSRR4 pKa = 11.84SFGTKK9 pKa = 9.35IGGQQMEE16 pKa = 5.1RR17 pKa = 11.84IYY19 pKa = 10.15CTYY22 pKa = 11.08ADD24 pKa = 3.94TEE26 pKa = 4.38VASLYY31 pKa = 9.49THH33 pKa = 5.98VQCPYY38 pKa = 10.44CNHH41 pKa = 5.96EE42 pKa = 4.11QNEE45 pKa = 4.42LGMDD49 pKa = 3.87EE50 pKa = 4.5CGKK53 pKa = 10.38IYY55 pKa = 10.6VIEE58 pKa = 4.45CEE60 pKa = 4.19EE61 pKa = 4.48CEE63 pKa = 4.35EE64 pKa = 3.99QYY66 pKa = 11.84EE67 pKa = 4.56MYY69 pKa = 10.74FDD71 pKa = 4.18ASS73 pKa = 3.38

Molecular weight: 8.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5MBM6|S5MBM6_9CAUD Terminase large subunit OS=Brevibacillus phage Jimmer1 OX=1296659 GN=JIMMER1_2 PE=3 SV=1
MM1 pKa = 7.19QSYY4 pKa = 10.74ILVKK8 pKa = 10.17VKK10 pKa = 10.45RR11 pKa = 11.84NRR13 pKa = 11.84PGSIVAQKK21 pKa = 10.31AQNSTNYY28 pKa = 9.13STFRR32 pKa = 11.84CYY34 pKa = 11.28DD35 pKa = 4.16FII37 pKa = 6.79

Molecular weight: 4.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

16387

27

677

159.1

18.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.858 ± 0.244

0.885 ± 0.115

6.151 ± 0.205

8.238 ± 0.288

3.991 ± 0.21

5.993 ± 0.201

1.642 ± 0.133

7.317 ± 0.211

8.714 ± 0.247

8.385 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.132

4.949 ± 0.219

3.039 ± 0.209

4.07 ± 0.206

4.699 ± 0.302

5.858 ± 0.214

5.791 ± 0.186

6.401 ± 0.223

1.275 ± 0.098

4.021 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski