Amino acid dipepetide frequency for Brevibacillus phage Jimmer1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.319AlaAla: 2.319 ± 0.31
0.793AlaCys: 0.793 ± 0.26
3.845AlaAsp: 3.845 ± 0.498
4.943AlaGlu: 4.943 ± 0.575
2.685AlaPhe: 2.685 ± 0.401
3.234AlaGly: 3.234 ± 0.461
0.854AlaHis: 0.854 ± 0.255
4.699AlaIle: 4.699 ± 0.568
5.737AlaLys: 5.737 ± 0.476
4.882AlaLeu: 4.882 ± 0.554
2.197AlaMet: 2.197 ± 0.428
2.929AlaAsn: 2.929 ± 0.395
1.282AlaPro: 1.282 ± 0.297
2.624AlaGln: 2.624 ± 0.318
2.746AlaArg: 2.746 ± 0.455
2.502AlaSer: 2.502 ± 0.425
2.502AlaThr: 2.502 ± 0.424
4.15AlaVal: 4.15 ± 0.601
1.343AlaTrp: 1.343 ± 0.314
2.319AlaTyr: 2.319 ± 0.332
0.0AlaXaa: 0.0 ± 0.0
Cys
0.183CysAla: 0.183 ± 0.11
0.244CysCys: 0.244 ± 0.159
0.732CysAsp: 0.732 ± 0.213
0.976CysGlu: 0.976 ± 0.268
0.427CysPhe: 0.427 ± 0.141
0.61CysGly: 0.61 ± 0.24
0.183CysHis: 0.183 ± 0.107
0.793CysIle: 0.793 ± 0.241
0.427CysLys: 0.427 ± 0.172
0.366CysLeu: 0.366 ± 0.153
0.0CysMet: 0.0 ± 0.0
0.61CysAsn: 0.61 ± 0.216
0.427CysPro: 0.427 ± 0.182
0.061CysGln: 0.061 ± 0.065
0.61CysArg: 0.61 ± 0.241
0.366CysSer: 0.366 ± 0.137
0.671CysThr: 0.671 ± 0.186
0.488CysVal: 0.488 ± 0.164
0.183CysTrp: 0.183 ± 0.164
0.488CysTyr: 0.488 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
3.173AspAla: 3.173 ± 0.414
0.427AspCys: 0.427 ± 0.193
3.479AspAsp: 3.479 ± 0.501
5.615AspGlu: 5.615 ± 0.586
2.99AspPhe: 2.99 ± 0.406
3.54AspGly: 3.54 ± 0.506
0.976AspHis: 0.976 ± 0.238
4.516AspIle: 4.516 ± 0.504
5.554AspLys: 5.554 ± 0.673
5.065AspLeu: 5.065 ± 0.555
1.77AspMet: 1.77 ± 0.277
3.234AspAsn: 3.234 ± 0.493
2.502AspPro: 2.502 ± 0.527
2.075AspGln: 2.075 ± 0.336
2.319AspArg: 2.319 ± 0.312
3.967AspSer: 3.967 ± 0.478
2.868AspThr: 2.868 ± 0.429
3.723AspVal: 3.723 ± 0.476
0.793AspTrp: 0.793 ± 0.212
2.929AspTyr: 2.929 ± 0.41
0.0AspXaa: 0.0 ± 0.0
Glu
4.516GluAla: 4.516 ± 0.464
0.732GluCys: 0.732 ± 0.242
3.906GluAsp: 3.906 ± 0.553
7.018GluGlu: 7.018 ± 0.816
3.662GluPhe: 3.662 ± 0.473
4.272GluGly: 4.272 ± 0.482
2.014GluHis: 2.014 ± 0.358
6.286GluIle: 6.286 ± 0.565
8.117GluLys: 8.117 ± 0.658
7.323GluLeu: 7.323 ± 0.791
2.746GluMet: 2.746 ± 0.363
2.319GluAsn: 2.319 ± 0.341
1.709GluPro: 1.709 ± 0.311
4.699GluGln: 4.699 ± 0.592
4.943GluArg: 4.943 ± 0.586
3.967GluSer: 3.967 ± 0.418
4.76GluThr: 4.76 ± 0.545
5.92GluVal: 5.92 ± 0.548
1.16GluTrp: 1.16 ± 0.279
2.319GluTyr: 2.319 ± 0.455
0.0GluXaa: 0.0 ± 0.0
Phe
2.197PheAla: 2.197 ± 0.339
0.305PheCys: 0.305 ± 0.147
3.173PheAsp: 3.173 ± 0.4
3.479PheGlu: 3.479 ± 0.474
1.648PhePhe: 1.648 ± 0.376
2.441PheGly: 2.441 ± 0.331
0.549PheHis: 0.549 ± 0.196
3.173PheIle: 3.173 ± 0.509
3.112PheLys: 3.112 ± 0.464
3.967PheLeu: 3.967 ± 0.454
0.854PheMet: 0.854 ± 0.194
1.526PheAsn: 1.526 ± 0.527
0.732PhePro: 0.732 ± 0.198
1.465PheGln: 1.465 ± 0.26
1.587PheArg: 1.587 ± 0.297
2.685PheSer: 2.685 ± 0.373
2.319PheThr: 2.319 ± 0.328
2.746PheVal: 2.746 ± 0.48
0.61PheTrp: 0.61 ± 0.175
1.343PheTyr: 1.343 ± 0.306
0.0PheXaa: 0.0 ± 0.0
Gly
3.906GlyAla: 3.906 ± 0.658
0.366GlyCys: 0.366 ± 0.181
4.028GlyAsp: 4.028 ± 0.526
4.15GlyGlu: 4.15 ± 0.464
2.014GlyPhe: 2.014 ± 0.493
3.357GlyGly: 3.357 ± 0.45
0.732GlyHis: 0.732 ± 0.231
3.845GlyIle: 3.845 ± 0.474
5.676GlyLys: 5.676 ± 0.499
4.333GlyLeu: 4.333 ± 0.535
1.709GlyMet: 1.709 ± 0.362
3.601GlyAsn: 3.601 ± 0.486
0.976GlyPro: 0.976 ± 0.22
1.892GlyGln: 1.892 ± 0.359
3.295GlyArg: 3.295 ± 0.53
3.479GlySer: 3.479 ± 0.473
3.479GlyThr: 3.479 ± 0.438
5.187GlyVal: 5.187 ± 0.633
0.854GlyTrp: 0.854 ± 0.244
3.051GlyTyr: 3.051 ± 0.385
0.0GlyXaa: 0.0 ± 0.0
His
0.793HisAla: 0.793 ± 0.209
0.122HisCys: 0.122 ± 0.089
0.976HisAsp: 0.976 ± 0.23
1.465HisGlu: 1.465 ± 0.286
0.854HisPhe: 0.854 ± 0.251
0.854HisGly: 0.854 ± 0.206
0.366HisHis: 0.366 ± 0.163
1.404HisIle: 1.404 ± 0.283
0.854HisLys: 0.854 ± 0.26
1.037HisLeu: 1.037 ± 0.202
0.122HisMet: 0.122 ± 0.085
1.098HisAsn: 1.098 ± 0.225
0.854HisPro: 0.854 ± 0.178
0.61HisGln: 0.61 ± 0.18
0.549HisArg: 0.549 ± 0.197
0.671HisSer: 0.671 ± 0.219
1.098HisThr: 1.098 ± 0.259
1.16HisVal: 1.16 ± 0.276
0.305HisTrp: 0.305 ± 0.136
1.221HisTyr: 1.221 ± 0.28
0.0HisXaa: 0.0 ± 0.0
Ile
5.309IleAla: 5.309 ± 0.669
0.549IleCys: 0.549 ± 0.173
3.906IleAsp: 3.906 ± 0.524
6.53IleGlu: 6.53 ± 0.663
2.075IlePhe: 2.075 ± 0.377
4.272IleGly: 4.272 ± 0.571
1.648IleHis: 1.648 ± 0.324
3.723IleIle: 3.723 ± 0.425
6.835IleLys: 6.835 ± 0.579
6.103IleLeu: 6.103 ± 0.585
1.282IleMet: 1.282 ± 0.33
4.089IleAsn: 4.089 ± 0.537
2.624IlePro: 2.624 ± 0.439
2.563IleGln: 2.563 ± 0.386
3.295IleArg: 3.295 ± 0.387
5.065IleSer: 5.065 ± 0.496
4.455IleThr: 4.455 ± 0.517
5.004IleVal: 5.004 ± 0.436
0.549IleTrp: 0.549 ± 0.204
3.295IleTyr: 3.295 ± 0.606
0.0IleXaa: 0.0 ± 0.0
Lys
5.37LysAla: 5.37 ± 0.617
0.976LysCys: 0.976 ± 0.232
4.394LysAsp: 4.394 ± 0.467
7.812LysGlu: 7.812 ± 0.665
2.685LysPhe: 2.685 ± 0.432
5.615LysGly: 5.615 ± 0.599
1.587LysHis: 1.587 ± 0.327
5.981LysIle: 5.981 ± 0.653
8.056LysLys: 8.056 ± 0.698
7.14LysLeu: 7.14 ± 0.688
3.051LysMet: 3.051 ± 0.475
4.699LysAsn: 4.699 ± 0.661
2.502LysPro: 2.502 ± 0.345
4.089LysGln: 4.089 ± 0.579
5.126LysArg: 5.126 ± 0.609
4.272LysSer: 4.272 ± 0.554
4.516LysThr: 4.516 ± 0.504
5.431LysVal: 5.431 ± 0.511
1.037LysTrp: 1.037 ± 0.251
2.807LysTyr: 2.807 ± 0.47
0.0LysXaa: 0.0 ± 0.0
Leu
6.164LeuAla: 6.164 ± 0.619
0.915LeuCys: 0.915 ± 0.237
6.042LeuAsp: 6.042 ± 0.62
7.201LeuGlu: 7.201 ± 0.697
3.54LeuPhe: 3.54 ± 0.532
4.394LeuGly: 4.394 ± 0.425
0.976LeuHis: 0.976 ± 0.237
6.164LeuIle: 6.164 ± 0.655
6.408LeuLys: 6.408 ± 0.673
6.774LeuLeu: 6.774 ± 0.65
2.38LeuMet: 2.38 ± 0.412
4.821LeuAsn: 4.821 ± 0.469
2.319LeuPro: 2.319 ± 0.334
3.723LeuGln: 3.723 ± 0.514
3.601LeuArg: 3.601 ± 0.438
6.042LeuSer: 6.042 ± 0.548
4.943LeuThr: 4.943 ± 0.527
3.967LeuVal: 3.967 ± 0.44
0.793LeuTrp: 0.793 ± 0.228
2.685LeuTyr: 2.685 ± 0.361
0.0LeuXaa: 0.0 ± 0.0
Met
2.014MetAla: 2.014 ± 0.346
0.122MetCys: 0.122 ± 0.084
1.465MetAsp: 1.465 ± 0.271
1.953MetGlu: 1.953 ± 0.329
1.037MetPhe: 1.037 ± 0.323
1.404MetGly: 1.404 ± 0.298
0.244MetHis: 0.244 ± 0.121
1.465MetIle: 1.465 ± 0.328
2.685MetLys: 2.685 ± 0.425
1.709MetLeu: 1.709 ± 0.374
1.282MetMet: 1.282 ± 0.26
1.404MetAsn: 1.404 ± 0.25
1.037MetPro: 1.037 ± 0.319
1.404MetGln: 1.404 ± 0.292
1.709MetArg: 1.709 ± 0.331
2.075MetSer: 2.075 ± 0.31
1.77MetThr: 1.77 ± 0.345
1.343MetVal: 1.343 ± 0.228
0.122MetTrp: 0.122 ± 0.069
0.976MetTyr: 0.976 ± 0.255
0.0MetXaa: 0.0 ± 0.0
Asn
1.892AsnAla: 1.892 ± 0.327
0.427AsnCys: 0.427 ± 0.156
2.319AsnAsp: 2.319 ± 0.437
4.028AsnGlu: 4.028 ± 0.467
1.892AsnPhe: 1.892 ± 0.277
3.662AsnGly: 3.662 ± 0.505
0.915AsnHis: 0.915 ± 0.232
3.723AsnIle: 3.723 ± 0.531
5.187AsnLys: 5.187 ± 0.562
4.394AsnLeu: 4.394 ± 0.5
1.16AsnMet: 1.16 ± 0.26
2.319AsnAsn: 2.319 ± 0.424
2.563AsnPro: 2.563 ± 0.633
2.014AsnGln: 2.014 ± 0.334
2.441AsnArg: 2.441 ± 0.371
2.746AsnSer: 2.746 ± 0.364
2.807AsnThr: 2.807 ± 0.387
2.38AsnVal: 2.38 ± 0.341
0.61AsnTrp: 0.61 ± 0.194
2.014AsnTyr: 2.014 ± 0.43
0.0AsnXaa: 0.0 ± 0.0
Pro
1.404ProAla: 1.404 ± 0.32
0.305ProCys: 0.305 ± 0.124
2.929ProAsp: 2.929 ± 0.438
2.136ProGlu: 2.136 ± 0.433
1.892ProPhe: 1.892 ± 0.342
1.282ProGly: 1.282 ± 0.308
0.427ProHis: 0.427 ± 0.155
2.563ProIle: 2.563 ± 0.331
2.075ProLys: 2.075 ± 0.479
2.136ProLeu: 2.136 ± 0.331
0.366ProMet: 0.366 ± 0.146
2.075ProAsn: 2.075 ± 0.499
0.976ProPro: 0.976 ± 0.315
1.098ProGln: 1.098 ± 0.274
1.282ProArg: 1.282 ± 0.3
2.197ProSer: 2.197 ± 0.299
2.014ProThr: 2.014 ± 0.368
1.709ProVal: 1.709 ± 0.339
0.427ProTrp: 0.427 ± 0.166
1.098ProTyr: 1.098 ± 0.245
0.0ProXaa: 0.0 ± 0.0
Gln
2.99GlnAla: 2.99 ± 0.458
0.244GlnCys: 0.244 ± 0.131
1.831GlnAsp: 1.831 ± 0.362
3.112GlnGlu: 3.112 ± 0.353
1.16GlnPhe: 1.16 ± 0.279
2.075GlnGly: 2.075 ± 0.358
0.793GlnHis: 0.793 ± 0.199
3.54GlnIle: 3.54 ± 0.496
3.112GlnLys: 3.112 ± 0.443
5.248GlnLeu: 5.248 ± 0.532
0.854GlnMet: 0.854 ± 0.238
2.319GlnAsn: 2.319 ± 0.408
1.098GlnPro: 1.098 ± 0.224
1.648GlnGln: 1.648 ± 0.378
1.953GlnArg: 1.953 ± 0.387
2.197GlnSer: 2.197 ± 0.373
2.441GlnThr: 2.441 ± 0.351
2.319GlnVal: 2.319 ± 0.389
0.427GlnTrp: 0.427 ± 0.164
1.343GlnTyr: 1.343 ± 0.329
0.0GlnXaa: 0.0 ± 0.0
Arg
2.136ArgAla: 2.136 ± 0.346
0.671ArgCys: 0.671 ± 0.217
1.831ArgAsp: 1.831 ± 0.318
4.272ArgGlu: 4.272 ± 0.428
1.953ArgPhe: 1.953 ± 0.326
3.418ArgGly: 3.418 ± 0.438
0.976ArgHis: 0.976 ± 0.313
3.845ArgIle: 3.845 ± 0.427
4.516ArgLys: 4.516 ± 0.646
3.967ArgLeu: 3.967 ± 0.618
1.953ArgMet: 1.953 ± 0.314
1.648ArgAsn: 1.648 ± 0.31
1.282ArgPro: 1.282 ± 0.242
1.953ArgGln: 1.953 ± 0.35
2.014ArgArg: 2.014 ± 0.377
2.014ArgSer: 2.014 ± 0.431
2.685ArgThr: 2.685 ± 0.465
3.112ArgVal: 3.112 ± 0.445
0.61ArgTrp: 0.61 ± 0.161
2.136ArgTyr: 2.136 ± 0.354
0.0ArgXaa: 0.0 ± 0.0
Ser
2.868SerAla: 2.868 ± 0.524
0.122SerCys: 0.122 ± 0.095
4.455SerAsp: 4.455 ± 0.462
4.15SerGlu: 4.15 ± 0.414
3.723SerPhe: 3.723 ± 0.567
3.418SerGly: 3.418 ± 0.384
0.915SerHis: 0.915 ± 0.225
4.638SerIle: 4.638 ± 0.518
5.126SerLys: 5.126 ± 0.622
5.126SerLeu: 5.126 ± 0.498
1.587SerMet: 1.587 ± 0.32
2.502SerAsn: 2.502 ± 0.362
1.892SerPro: 1.892 ± 0.29
2.014SerGln: 2.014 ± 0.343
1.953SerArg: 1.953 ± 0.342
3.784SerSer: 3.784 ± 0.424
3.845SerThr: 3.845 ± 0.566
3.479SerVal: 3.479 ± 0.363
0.793SerTrp: 0.793 ± 0.212
2.197SerTyr: 2.197 ± 0.398
0.0SerXaa: 0.0 ± 0.0
Thr
3.845ThrAla: 3.845 ± 0.555
0.427ThrCys: 0.427 ± 0.148
3.54ThrAsp: 3.54 ± 0.508
4.272ThrGlu: 4.272 ± 0.423
1.892ThrPhe: 1.892 ± 0.339
4.211ThrGly: 4.211 ± 0.528
0.976ThrHis: 0.976 ± 0.193
4.028ThrIle: 4.028 ± 0.549
5.004ThrLys: 5.004 ± 0.59
5.676ThrLeu: 5.676 ± 0.502
1.16ThrMet: 1.16 ± 0.247
2.746ThrAsn: 2.746 ± 0.418
2.258ThrPro: 2.258 ± 0.317
2.258ThrGln: 2.258 ± 0.333
1.831ThrArg: 1.831 ± 0.313
3.418ThrSer: 3.418 ± 0.496
3.112ThrThr: 3.112 ± 0.35
3.967ThrVal: 3.967 ± 0.538
0.793ThrTrp: 0.793 ± 0.228
2.502ThrTyr: 2.502 ± 0.372
0.0ThrXaa: 0.0 ± 0.0
Val
4.821ValAla: 4.821 ± 0.606
0.427ValCys: 0.427 ± 0.156
4.638ValAsp: 4.638 ± 0.563
4.516ValGlu: 4.516 ± 0.512
1.77ValPhe: 1.77 ± 0.323
4.272ValGly: 4.272 ± 0.504
0.549ValHis: 0.549 ± 0.183
5.004ValIle: 5.004 ± 0.545
4.455ValLys: 4.455 ± 0.556
3.845ValLeu: 3.845 ± 0.553
1.648ValMet: 1.648 ± 0.32
3.234ValAsn: 3.234 ± 0.405
1.892ValPro: 1.892 ± 0.262
2.685ValGln: 2.685 ± 0.394
3.173ValArg: 3.173 ± 0.43
4.333ValSer: 4.333 ± 0.416
4.577ValThr: 4.577 ± 0.571
4.089ValVal: 4.089 ± 0.583
0.549ValTrp: 0.549 ± 0.178
3.54ValTyr: 3.54 ± 0.427
0.0ValXaa: 0.0 ± 0.0
Trp
0.488TrpAla: 0.488 ± 0.168
0.122TrpCys: 0.122 ± 0.102
0.671TrpAsp: 0.671 ± 0.206
1.221TrpGlu: 1.221 ± 0.235
0.549TrpPhe: 0.549 ± 0.159
0.793TrpGly: 0.793 ± 0.206
0.183TrpHis: 0.183 ± 0.107
0.671TrpIle: 0.671 ± 0.196
1.648TrpLys: 1.648 ± 0.294
1.404TrpLeu: 1.404 ± 0.265
0.183TrpMet: 0.183 ± 0.115
0.244TrpAsn: 0.244 ± 0.119
0.244TrpPro: 0.244 ± 0.177
0.427TrpGln: 0.427 ± 0.151
0.61TrpArg: 0.61 ± 0.176
0.549TrpSer: 0.549 ± 0.163
1.098TrpThr: 1.098 ± 0.229
0.976TrpVal: 0.976 ± 0.242
0.366TrpTrp: 0.366 ± 0.165
0.305TrpTyr: 0.305 ± 0.132
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.197TyrAla: 2.197 ± 0.331
0.549TyrCys: 0.549 ± 0.203
3.357TyrAsp: 3.357 ± 0.409
3.54TyrGlu: 3.54 ± 0.531
1.465TyrPhe: 1.465 ± 0.288
2.807TyrGly: 2.807 ± 0.501
0.549TyrHis: 0.549 ± 0.153
3.112TyrIle: 3.112 ± 0.398
2.563TyrLys: 2.563 ± 0.417
3.357TyrLeu: 3.357 ± 0.47
0.854TyrMet: 0.854 ± 0.211
2.075TyrAsn: 2.075 ± 0.358
1.221TyrPro: 1.221 ± 0.271
1.404TyrGln: 1.404 ± 0.259
1.953TyrArg: 1.953 ± 0.405
2.258TyrSer: 2.258 ± 0.458
1.953TyrThr: 1.953 ± 0.419
2.868TyrVal: 2.868 ± 0.508
0.427TyrTrp: 0.427 ± 0.171
1.648TyrTyr: 1.648 ± 0.35
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 103 proteins (16387 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski