Anaerobacillus arseniciselenatis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Anaerobacillus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3587 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1S2LQ82|A0A1S2LQ82_9BACI Uncharacterized protein OS=Anaerobacillus arseniciselenatis OX=85682 GN=BKP35_06265 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.18KK3 pKa = 9.77YY4 pKa = 10.72LKK6 pKa = 10.01RR7 pKa = 11.84LSIAAGLSLTLLAAGCGSDD26 pKa = 3.62ATTGDD31 pKa = 3.88DD32 pKa = 3.67SSVGGSLDD40 pKa = 3.57YY41 pKa = 10.95TITGIDD47 pKa = 3.18AGAGIMAATEE57 pKa = 4.03EE58 pKa = 4.56AIEE61 pKa = 4.42AYY63 pKa = 10.26GLEE66 pKa = 4.72DD67 pKa = 3.85YY68 pKa = 11.04NLQTSSSAAMTAALDD83 pKa = 3.53AAYY86 pKa = 9.32TNEE89 pKa = 3.9EE90 pKa = 4.59PIVVTGWTPHH100 pKa = 4.83WKK102 pKa = 9.0FAKK105 pKa = 10.28YY106 pKa = 9.77DD107 pKa = 3.76LKK109 pKa = 11.22YY110 pKa = 11.09LEE112 pKa = 5.21DD113 pKa = 3.82PQGIFGEE120 pKa = 4.53SEE122 pKa = 4.35VIHH125 pKa = 6.19TIARR129 pKa = 11.84AGLDD133 pKa = 3.4TDD135 pKa = 4.33HH136 pKa = 7.36PSAYY140 pKa = 9.41QVLEE144 pKa = 3.93NFYY147 pKa = 10.43WEE149 pKa = 4.09QDD151 pKa = 3.37DD152 pKa = 4.31MGAIMIDD159 pKa = 3.82VNEE162 pKa = 4.5GADD165 pKa = 3.81PVDD168 pKa = 4.76AAADD172 pKa = 3.52WVAANQDD179 pKa = 4.03KK180 pKa = 10.75VSEE183 pKa = 4.19WTDD186 pKa = 3.52GVSHH190 pKa = 7.12VDD192 pKa = 3.14GDD194 pKa = 4.18QITLAFVAWDD204 pKa = 3.52SEE206 pKa = 4.26IASTHH211 pKa = 5.8MIGKK215 pKa = 8.69VLEE218 pKa = 4.95DD219 pKa = 2.59IGYY222 pKa = 9.74DD223 pKa = 3.5VEE225 pKa = 4.82LVSLEE230 pKa = 3.99AAGMWTAVATGSADD244 pKa = 3.88AIVAAWLPGTHH255 pKa = 6.5AAYY258 pKa = 10.94YY259 pKa = 10.18EE260 pKa = 4.27DD261 pKa = 3.98YY262 pKa = 11.08KK263 pKa = 11.45EE264 pKa = 4.85DD265 pKa = 4.92FIDD268 pKa = 4.76LGVNLEE274 pKa = 4.07GAGIGLVVPAYY285 pKa = 9.9MDD287 pKa = 3.41IDD289 pKa = 4.58SIEE292 pKa = 4.26DD293 pKa = 3.5LNNN296 pKa = 3.12

Molecular weight:
31.54 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1S2LIU4|A0A1S2LIU4_9BACI Uncharacterized protein OS=Anaerobacillus arseniciselenatis OX=85682 GN=BKP35_10905 PE=4 SV=1
MM1 pKa = 6.37TTTVRR6 pKa = 11.84RR7 pKa = 11.84LVFFTALIAIWEE19 pKa = 4.64SIFRR23 pKa = 11.84LNDD26 pKa = 2.5IFTFRR31 pKa = 11.84DD32 pKa = 3.24TRR34 pKa = 11.84FFPSPSGAIEE44 pKa = 3.62QLYY47 pKa = 10.45IGFFQSGILSSALITSLQRR66 pKa = 11.84ISVGFALALIIGTALGILLGQSKK89 pKa = 10.09LADD92 pKa = 3.62EE93 pKa = 5.22TIGSLVIALQSIPSIVWLPIALIAFGQGSTAIIFIIVLGGTWAMTMNVRR142 pKa = 11.84MGIKK146 pKa = 9.74NVQPLLIRR154 pKa = 11.84AARR157 pKa = 11.84TMGYY161 pKa = 9.79KK162 pKa = 10.42GSEE165 pKa = 4.29LVWKK169 pKa = 10.59VMLPASIPAALTGARR184 pKa = 11.84LAWAFGWRR192 pKa = 11.84ALMAGEE198 pKa = 3.83LLGRR202 pKa = 11.84GGLGRR207 pKa = 11.84TLLDD211 pKa = 3.53ARR213 pKa = 11.84DD214 pKa = 4.25MFNMDD219 pKa = 3.95LVIAIMVIISIIGLIVEE236 pKa = 4.44YY237 pKa = 10.72LVFAPLEE244 pKa = 4.35KK245 pKa = 10.24KK246 pKa = 10.87VMTRR250 pKa = 11.84WGLSKK255 pKa = 10.97

Molecular weight:
27.81 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3587

0

3587

1023233

26

1847

285.3

32.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.456 ± 0.05

0.789 ± 0.014

5.097 ± 0.033

7.877 ± 0.05

4.756 ± 0.04

6.589 ± 0.041

2.048 ± 0.02

8.131 ± 0.046

7.045 ± 0.049

9.732 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.019

4.739 ± 0.033

3.46 ± 0.029

3.687 ± 0.029

3.94 ± 0.031

5.886 ± 0.031

5.398 ± 0.03

7.255 ± 0.041

0.971 ± 0.015

3.486 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski