Anaerobacillus arseniciselenatis
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3587 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S2LQ82|A0A1S2LQ82_9BACI Uncharacterized protein OS=Anaerobacillus arseniciselenatis OX=85682 GN=BKP35_06265 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.18 KK3 pKa = 9.77 YY4 pKa = 10.72 LKK6 pKa = 10.01 RR7 pKa = 11.84 LSIAAGLSLTLLAAGCGSDD26 pKa = 3.62 ATTGDD31 pKa = 3.88 DD32 pKa = 3.67 SSVGGSLDD40 pKa = 3.57 YY41 pKa = 10.95 TITGIDD47 pKa = 3.18 AGAGIMAATEE57 pKa = 4.03 EE58 pKa = 4.56 AIEE61 pKa = 4.42 AYY63 pKa = 10.26 GLEE66 pKa = 4.72 DD67 pKa = 3.85 YY68 pKa = 11.04 NLQTSSSAAMTAALDD83 pKa = 3.53 AAYY86 pKa = 9.32 TNEE89 pKa = 3.9 EE90 pKa = 4.59 PIVVTGWTPHH100 pKa = 4.83 WKK102 pKa = 9.0 FAKK105 pKa = 10.28 YY106 pKa = 9.77 DD107 pKa = 3.76 LKK109 pKa = 11.22 YY110 pKa = 11.09 LEE112 pKa = 5.21 DD113 pKa = 3.82 PQGIFGEE120 pKa = 4.53 SEE122 pKa = 4.35 VIHH125 pKa = 6.19 TIARR129 pKa = 11.84 AGLDD133 pKa = 3.4 TDD135 pKa = 4.33 HH136 pKa = 7.36 PSAYY140 pKa = 9.41 QVLEE144 pKa = 3.93 NFYY147 pKa = 10.43 WEE149 pKa = 4.09 QDD151 pKa = 3.37 DD152 pKa = 4.31 MGAIMIDD159 pKa = 3.82 VNEE162 pKa = 4.5 GADD165 pKa = 3.81 PVDD168 pKa = 4.76 AAADD172 pKa = 3.52 WVAANQDD179 pKa = 4.03 KK180 pKa = 10.75 VSEE183 pKa = 4.19 WTDD186 pKa = 3.52 GVSHH190 pKa = 7.12 VDD192 pKa = 3.14 GDD194 pKa = 4.18 QITLAFVAWDD204 pKa = 3.52 SEE206 pKa = 4.26 IASTHH211 pKa = 5.8 MIGKK215 pKa = 8.69 VLEE218 pKa = 4.95 DD219 pKa = 2.59 IGYY222 pKa = 9.74 DD223 pKa = 3.5 VEE225 pKa = 4.82 LVSLEE230 pKa = 3.99 AAGMWTAVATGSADD244 pKa = 3.88 AIVAAWLPGTHH255 pKa = 6.5 AAYY258 pKa = 10.94 YY259 pKa = 10.18 EE260 pKa = 4.27 DD261 pKa = 3.98 YY262 pKa = 11.08 KK263 pKa = 11.45 EE264 pKa = 4.85 DD265 pKa = 4.92 FIDD268 pKa = 4.76 LGVNLEE274 pKa = 4.07 GAGIGLVVPAYY285 pKa = 9.9 MDD287 pKa = 3.41 IDD289 pKa = 4.58 SIEE292 pKa = 4.26 DD293 pKa = 3.5 LNNN296 pKa = 3.12
Molecular weight: 31.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.528
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.567
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.088
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.872
Patrickios 0.769
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.771
Protein with the highest isoelectric point:
>tr|A0A1S2LIU4|A0A1S2LIU4_9BACI Uncharacterized protein OS=Anaerobacillus arseniciselenatis OX=85682 GN=BKP35_10905 PE=4 SV=1
MM1 pKa = 6.37 TTTVRR6 pKa = 11.84 RR7 pKa = 11.84 LVFFTALIAIWEE19 pKa = 4.64 SIFRR23 pKa = 11.84 LNDD26 pKa = 2.5 IFTFRR31 pKa = 11.84 DD32 pKa = 3.24 TRR34 pKa = 11.84 FFPSPSGAIEE44 pKa = 3.62 QLYY47 pKa = 10.45 IGFFQSGILSSALITSLQRR66 pKa = 11.84 ISVGFALALIIGTALGILLGQSKK89 pKa = 10.09 LADD92 pKa = 3.62 EE93 pKa = 5.22 TIGSLVIALQSIPSIVWLPIALIAFGQGSTAIIFIIVLGGTWAMTMNVRR142 pKa = 11.84 MGIKK146 pKa = 9.74 NVQPLLIRR154 pKa = 11.84 AARR157 pKa = 11.84 TMGYY161 pKa = 9.79 KK162 pKa = 10.42 GSEE165 pKa = 4.29 LVWKK169 pKa = 10.59 VMLPASIPAALTGARR184 pKa = 11.84 LAWAFGWRR192 pKa = 11.84 ALMAGEE198 pKa = 3.83 LLGRR202 pKa = 11.84 GGLGRR207 pKa = 11.84 TLLDD211 pKa = 3.53 ARR213 pKa = 11.84 DD214 pKa = 4.25 MFNMDD219 pKa = 3.95 LVIAIMVIISIIGLIVEE236 pKa = 4.44 YY237 pKa = 10.72 LVFAPLEE244 pKa = 4.35 KK245 pKa = 10.24 KK246 pKa = 10.87 VMTRR250 pKa = 11.84 WGLSKK255 pKa = 10.97
Molecular weight: 27.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.619
IPC_protein 10.409
Toseland 10.701
ProMoST 10.452
Dawson 10.789
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 11.008
Grimsley 10.833
Solomon 10.921
Lehninger 10.891
Nozaki 10.672
DTASelect 10.496
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.716
Patrickios 10.745
IPC_peptide 10.921
IPC2_peptide 9.194
IPC2.peptide.svr19 8.801
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3587
0
3587
1023233
26
1847
285.3
32.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.456 ± 0.05
0.789 ± 0.014
5.097 ± 0.033
7.877 ± 0.05
4.756 ± 0.04
6.589 ± 0.041
2.048 ± 0.02
8.131 ± 0.046
7.045 ± 0.049
9.732 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.659 ± 0.019
4.739 ± 0.033
3.46 ± 0.029
3.687 ± 0.029
3.94 ± 0.031
5.886 ± 0.031
5.398 ± 0.03
7.255 ± 0.041
0.971 ± 0.015
3.486 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here