Rhodobacter vinaykumarii
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3255 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N7KQQ1|A0A1N7KQQ1_9RHOB Uncharacterized protein OS=Rhodobacter vinaykumarii OX=407234 GN=SAMN05421795_10255 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.41 KK3 pKa = 10.44 LLLASTALVMTAGVASAEE21 pKa = 4.11 MTIGGSAEE29 pKa = 3.9 MGFQGGDD36 pKa = 3.37 FYY38 pKa = 11.81 GVGTDD43 pKa = 4.8 DD44 pKa = 5.67 LSFHH48 pKa = 6.64 QDD50 pKa = 3.03 YY51 pKa = 11.23 SIDD54 pKa = 3.42 IKK56 pKa = 11.2 GTGTTDD62 pKa = 2.69 AGLEE66 pKa = 4.03 FGFTFSLQGDD76 pKa = 4.11 GNVRR80 pKa = 11.84 TRR82 pKa = 11.84 NDD84 pKa = 2.88 IAGDD88 pKa = 3.63 NEE90 pKa = 4.56 TVFIGGAFGKK100 pKa = 8.77 LTMGDD105 pKa = 3.14 TDD107 pKa = 5.08 GAFDD111 pKa = 3.43 WAMTEE116 pKa = 3.98 VNLAGGSIADD126 pKa = 4.78 DD127 pKa = 3.88 EE128 pKa = 4.76 TTHH131 pKa = 7.37 GGFNGNAAFDD141 pKa = 4.16 GQNDD145 pKa = 4.08 DD146 pKa = 3.06 QVLRR150 pKa = 11.84 YY151 pKa = 8.7 EE152 pKa = 4.32 YY153 pKa = 11.61 AMGAFGFALSYY164 pKa = 10.65 EE165 pKa = 4.23 QFAQGQSVPGVDD177 pKa = 3.86 ADD179 pKa = 3.96 DD180 pKa = 4.9 SIGVGLKK187 pKa = 10.58 YY188 pKa = 9.35 STQMSTVALDD198 pKa = 3.74 FGLGYY203 pKa = 9.8 QQGAATAIGPYY214 pKa = 9.38 TLVSRR219 pKa = 11.84 TNGDD223 pKa = 3.44 VIGASVKK230 pKa = 10.75 ADD232 pKa = 3.87 FGNGFLAGLNYY243 pKa = 10.36 SSYY246 pKa = 11.25 DD247 pKa = 3.3 FEE249 pKa = 6.25 ARR251 pKa = 11.84 DD252 pKa = 3.64 VTGVLTNTPDD262 pKa = 3.43 ATHH265 pKa = 6.53 MGLGVAYY272 pKa = 6.98 TTGPWTVAANYY283 pKa = 9.61 GKK285 pKa = 10.89 YY286 pKa = 10.48 DD287 pKa = 3.46 VDD289 pKa = 3.85 AVNADD294 pKa = 3.29 VTGYY298 pKa = 10.96 GLVVNYY304 pKa = 10.24 DD305 pKa = 3.56 LGGGAVVQAGYY316 pKa = 10.39 GATDD320 pKa = 4.11 FEE322 pKa = 5.27 APAANDD328 pKa = 3.65 TEE330 pKa = 4.81 SYY332 pKa = 10.43 SIGVAMSFF340 pKa = 3.82
Molecular weight: 35.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.478
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.694
Rodwell 3.541
Grimsley 3.389
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.126
Thurlkill 3.541
EMBOSS 3.694
Sillero 3.846
Patrickios 0.604
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.777
Protein with the highest isoelectric point:
>tr|A0A1N7JQ12|A0A1N7JQ12_9RHOB Ferric uptake regulation protein OS=Rhodobacter vinaykumarii OX=407234 GN=fur PE=3 SV=1
MM1 pKa = 7.16 PRR3 pKa = 11.84 KK4 pKa = 9.4 RR5 pKa = 11.84 QAAMPRR11 pKa = 11.84 KK12 pKa = 9.26 SQAALPRR19 pKa = 11.84 GGQVAMPRR27 pKa = 11.84 IGQAAMPRR35 pKa = 11.84 GGHH38 pKa = 6.4 RR39 pKa = 11.84 ATPGNRR45 pKa = 11.84 ATPGNDD51 pKa = 2.82 APANRR56 pKa = 11.84 PAVARR61 pKa = 11.84 AGRR64 pKa = 11.84 ARR66 pKa = 3.45
Molecular weight: 6.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.436
IPC2_protein 10.935
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.281
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.018
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.126
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3255
0
3255
1023962
25
1967
314.6
33.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.465 ± 0.08
0.831 ± 0.015
5.777 ± 0.044
5.803 ± 0.047
3.404 ± 0.024
9.092 ± 0.054
2.037 ± 0.022
4.6 ± 0.031
2.41 ± 0.037
10.386 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.615 ± 0.022
2.091 ± 0.022
5.794 ± 0.042
2.827 ± 0.024
7.863 ± 0.045
4.519 ± 0.028
5.149 ± 0.03
7.074 ± 0.032
1.383 ± 0.018
1.88 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here