Dragonfly larvae associated circular virus-1
Average proteome isoelectric point is 8.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5U268|W5U268_9VIRU ATP-dependent helicase Rep OS=Dragonfly larvae associated circular virus-1 OX=1454021 PE=3 SV=1
MM1 pKa = 7.79 PLSDD5 pKa = 4.51 HH6 pKa = 7.2 RR7 pKa = 11.84 SPAPGSRR14 pKa = 11.84 LSVRR18 pKa = 11.84 RR19 pKa = 11.84 QGIFWMLTIPHH30 pKa = 6.75 HH31 pKa = 6.68 CFTPYY36 pKa = 10.62 LPPGCVWIRR45 pKa = 11.84 GQHH48 pKa = 5.08 EE49 pKa = 4.62 LSLSGYY55 pKa = 6.95 SHH57 pKa = 6.18 WQILVAMSTKK67 pKa = 10.55 SSLAQVKK74 pKa = 10.04 SVFGEE79 pKa = 3.93 QCHH82 pKa = 6.35 AEE84 pKa = 4.15 LSRR87 pKa = 11.84 SEE89 pKa = 4.24 SAQDD93 pKa = 3.78 YY94 pKa = 8.97 VWKK97 pKa = 10.35 EE98 pKa = 3.95 DD99 pKa = 3.43 TRR101 pKa = 11.84 VDD103 pKa = 3.55 CSQFEE108 pKa = 4.47 FGSKK112 pKa = 9.95 PIRR115 pKa = 11.84 RR116 pKa = 11.84 NSRR119 pKa = 11.84 VDD121 pKa = 3.23 WEE123 pKa = 4.97 SVWSAAKK130 pKa = 10.59 SGDD133 pKa = 3.72 LLQIPANVRR142 pKa = 11.84 VVSYY146 pKa = 8.57 RR147 pKa = 11.84 TLRR150 pKa = 11.84 AIKK153 pKa = 10.08 ADD155 pKa = 3.72 YY156 pKa = 9.16 EE157 pKa = 4.81 VPQSMEE163 pKa = 3.94 RR164 pKa = 11.84 TALCYY169 pKa = 9.29 WGPTGTGKK177 pKa = 10.08 SLRR180 pKa = 11.84 SSRR183 pKa = 11.84 EE184 pKa = 3.7 AGIDD188 pKa = 3.46 AYY190 pKa = 11.5 YY191 pKa = 10.71 KK192 pKa = 10.52 DD193 pKa = 3.98 PRR195 pKa = 11.84 TKK197 pKa = 10.0 FWCGYY202 pKa = 9.47 RR203 pKa = 11.84 GQQHH207 pKa = 6.08 VIVDD211 pKa = 4.07 EE212 pKa = 4.14 FRR214 pKa = 11.84 GAIDD218 pKa = 3.24 ISHH221 pKa = 7.84 LLRR224 pKa = 11.84 WLDD227 pKa = 3.73 RR228 pKa = 11.84 YY229 pKa = 10.14 PCYY232 pKa = 10.82 LEE234 pKa = 4.38 TKK236 pKa = 10.31 GSSTSNCVTKK246 pKa = 10.42 FWFTSNLHH254 pKa = 6.18 PDD256 pKa = 3.67 FWYY259 pKa = 10.14 PDD261 pKa = 3.66 LDD263 pKa = 3.77 EE264 pKa = 4.57 LTRR267 pKa = 11.84 LALIRR272 pKa = 11.84 RR273 pKa = 11.84 ISVLEE278 pKa = 3.67 ITEE281 pKa = 5.04 LDD283 pKa = 3.57 TSLLPP288 pKa = 4.48
Molecular weight: 33.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.38
IPC2_protein 7.629
IPC_protein 7.512
Toseland 7.132
ProMoST 8.126
Dawson 8.17
Bjellqvist 8.58
Wikipedia 8.083
Rodwell 8.17
Grimsley 7.176
Solomon 8.258
Lehninger 8.273
Nozaki 8.77
DTASelect 8.287
Thurlkill 8.331
EMBOSS 8.39
Sillero 8.668
Patrickios 4.202
IPC_peptide 8.244
IPC2_peptide 7.79
IPC2.peptide.svr19 7.73
Protein with the highest isoelectric point:
>tr|W5U268|W5U268_9VIRU ATP-dependent helicase Rep OS=Dragonfly larvae associated circular virus-1 OX=1454021 PE=3 SV=1
MM1 pKa = 7.38 TRR3 pKa = 11.84 TFNYY7 pKa = 8.73 GHH9 pKa = 7.55 PLRR12 pKa = 11.84 VVGTIAANQFYY23 pKa = 10.73 NKK25 pKa = 10.1 YY26 pKa = 8.3 IAPWKK31 pKa = 9.88 DD32 pKa = 3.01 RR33 pKa = 11.84 KK34 pKa = 10.57 HH35 pKa = 6.47 PDD37 pKa = 2.65 WLSYY41 pKa = 10.92 KK42 pKa = 10.1 PIDD45 pKa = 3.47 YY46 pKa = 10.91 SSIYY50 pKa = 10.39 KK51 pKa = 9.31 PDD53 pKa = 4.14 HH54 pKa = 6.17 GSKK57 pKa = 10.34 PLPKK61 pKa = 9.97 KK62 pKa = 10.59 KK63 pKa = 9.92 EE64 pKa = 4.01 STMGAEE70 pKa = 5.17 DD71 pKa = 3.52 IPIGVKK77 pKa = 10.12 KK78 pKa = 10.6 NVVSVHH84 pKa = 5.91 TGLKK88 pKa = 9.45 RR89 pKa = 11.84 LKK91 pKa = 10.82 GMVAGHH97 pKa = 6.17 ISYY100 pKa = 10.38 RR101 pKa = 11.84 DD102 pKa = 3.55 SYY104 pKa = 10.72 PALLVCDD111 pKa = 4.12 EE112 pKa = 5.08 GYY114 pKa = 11.18 QNVTAPVYY122 pKa = 10.55 IGTTSQFLTATSNGVASNRR141 pKa = 11.84 DD142 pKa = 3.59 SVSYY146 pKa = 10.97 ASWFALNPEE155 pKa = 3.96 QGIAAGSIISANTAPACDD173 pKa = 4.11 KK174 pKa = 10.9 IGLQSSTTYY183 pKa = 11.33 LDD185 pKa = 3.9 FKK187 pKa = 11.42 NDD189 pKa = 3.52 TTNICYY195 pKa = 10.22 YY196 pKa = 10.17 KK197 pKa = 10.0 VHH199 pKa = 8.01 IYY201 pKa = 7.98 MAKK204 pKa = 8.86 TDD206 pKa = 3.74 TNQTVLSEE214 pKa = 4.5 FNNSTGSDD222 pKa = 2.96 NLYY225 pKa = 10.69 NSNFAYY231 pKa = 8.55 GTGVGTVLPATSGNEE246 pKa = 3.59 QTNAYY251 pKa = 8.25 IASSTSQIEE260 pKa = 4.45 SCNMLPYY267 pKa = 10.32 TKK269 pKa = 9.69 ISSKK273 pKa = 10.59 RR274 pKa = 11.84 LVRR277 pKa = 11.84 SVWKK281 pKa = 10.03 KK282 pKa = 9.87 IKK284 pKa = 9.58 TISHH288 pKa = 5.74 VFSGGDD294 pKa = 2.94 AWKK297 pKa = 10.5 LIVNIKK303 pKa = 10.05 HH304 pKa = 5.35 NQFGLRR310 pKa = 11.84 EE311 pKa = 3.99 RR312 pKa = 11.84 LNTLSVVFPKK322 pKa = 10.96 GCVCFLFEE330 pKa = 4.2 MQGGTVYY337 pKa = 9.51 NTANSAFGYY346 pKa = 8.2 SACQMAVNITRR357 pKa = 11.84 KK358 pKa = 9.08 IHH360 pKa = 6.35 LSPMAIPNKK369 pKa = 9.43 RR370 pKa = 11.84 FEE372 pKa = 4.1 SRR374 pKa = 11.84 YY375 pKa = 8.87 VGAGRR380 pKa = 11.84 EE381 pKa = 4.11 FQGALLGTEE390 pKa = 3.7 KK391 pKa = 10.81 DD392 pKa = 3.59 LYY394 pKa = 11.26 HH395 pKa = 5.62 NTEE398 pKa = 3.99 VGAVPAKK405 pKa = 10.29 IVV407 pKa = 3.05
Molecular weight: 44.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.732
IPC2_protein 8.77
IPC_protein 8.639
Toseland 9.151
ProMoST 9.077
Dawson 9.516
Bjellqvist 9.326
Wikipedia 9.706
Rodwell 9.677
Grimsley 9.589
Solomon 9.545
Lehninger 9.487
Nozaki 9.37
DTASelect 9.268
Thurlkill 9.355
EMBOSS 9.619
Sillero 9.502
Patrickios 4.164
IPC_peptide 9.531
IPC2_peptide 8.053
IPC2.peptide.svr19 7.77
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
695
288
407
347.5
38.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.619 ± 0.868
2.158 ± 0.381
4.46 ± 0.674
4.029 ± 0.726
3.597 ± 0.077
6.763 ± 0.743
2.734 ± 0.241
5.612 ± 0.462
5.755 ± 1.191
7.482 ± 1.165
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.727 ± 0.208
4.604 ± 1.978
4.892 ± 0.408
3.309 ± 0.314
5.468 ± 1.763
9.928 ± 0.728
6.906 ± 0.831
6.619 ± 0.44
2.302 ± 0.933
5.036 ± 0.535
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here