Gongylonema pulchrum
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26895 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A183DPK0|A0A183DPK0_9BILA DUF2052 domain-containing protein OS=Gongylonema pulchrum OX=637853 GN=GPUH_LOCUS10641 PE=4 SV=1
MM1 pKa = 7.51 PADD4 pKa = 4.04 EE5 pKa = 5.16 VKK7 pKa = 10.06 MLQLRR12 pKa = 11.84 SFFAEE17 pKa = 3.88 EE18 pKa = 4.42 DD19 pKa = 3.68 DD20 pKa = 4.92 DD21 pKa = 5.27 GVVANVVLLVILLLLTEE38 pKa = 4.65 LFEE41 pKa = 4.6 IALADD46 pKa = 4.97 EE47 pKa = 4.98 IDD49 pKa = 4.16 CSDD52 pKa = 3.67 VLIVEE57 pKa = 4.46 VDD59 pKa = 3.19 DD60 pKa = 3.94 CTEE63 pKa = 3.92 HH64 pKa = 6.36 GRR66 pKa = 11.84 CGGEE70 pKa = 3.81 RR71 pKa = 11.84 TPLGAAISDD80 pKa = 3.69 AYY82 pKa = 10.12 YY83 pKa = 10.83 NRR85 pKa = 11.84 FIVVLLVILLLLTEE99 pKa = 4.65 LFEE102 pKa = 4.6 IALADD107 pKa = 4.97 EE108 pKa = 4.98 IDD110 pKa = 4.16 CSDD113 pKa = 3.67 VLIVEE118 pKa = 4.46 VDD120 pKa = 3.19 DD121 pKa = 3.94 CTEE124 pKa = 3.92 HH125 pKa = 6.36 GRR127 pKa = 11.84 CGGEE131 pKa = 3.81 RR132 pKa = 11.84 TPLGAAISDD141 pKa = 3.7 AYY143 pKa = 10.93 YY144 pKa = 11.23 NRR146 pKa = 11.84 FTT148 pKa = 4.43
Molecular weight: 16.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.579
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.617
Grimsley 3.49
Solomon 3.757
Lehninger 3.706
Nozaki 3.884
DTASelect 4.088
Thurlkill 3.63
EMBOSS 3.694
Sillero 3.91
Patrickios 1.036
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A3P6P130|A0A3P6P130_9BILA Tyrosine-protein phosphatase domain-containing protein OS=Gongylonema pulchrum OX=637853 GN=GPUH_LOCUS1469 PE=4 SV=1
MM1 pKa = 7.57 IFFHH5 pKa = 6.68 FLVSLFWLRR14 pKa = 11.84 IHH16 pKa = 7.06 IFAAKK21 pKa = 9.74 IPNHH25 pKa = 6.15 LLHH28 pKa = 7.22 LARR31 pKa = 11.84 HH32 pKa = 4.72 QQQYY36 pKa = 7.97 HH37 pKa = 4.32 HH38 pKa = 6.95 HH39 pKa = 6.85 RR40 pKa = 11.84 LRR42 pKa = 11.84 PCFLHH47 pKa = 5.03 WMSRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 NRR55 pKa = 11.84 QNRR58 pKa = 11.84 GKK60 pKa = 10.61 RR61 pKa = 11.84 IRR63 pKa = 11.84 NPRR66 pKa = 11.84 KK67 pKa = 9.57 RR68 pKa = 11.84 KK69 pKa = 9.43 LLRR72 pKa = 11.84 KK73 pKa = 8.97 AKK75 pKa = 9.81 KK76 pKa = 9.54 RR77 pKa = 11.84 RR78 pKa = 11.84 KK79 pKa = 8.6 KK80 pKa = 8.94 AQRR83 pKa = 11.84 RR84 pKa = 11.84 KK85 pKa = 8.62 GRR87 pKa = 11.84 RR88 pKa = 11.84 RR89 pKa = 11.84 RR90 pKa = 11.84 SRR92 pKa = 11.84 KK93 pKa = 9.26 KK94 pKa = 8.64 KK95 pKa = 7.63 AQRR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 KK101 pKa = 7.09 VQRR104 pKa = 11.84 RR105 pKa = 11.84 KK106 pKa = 8.93 ARR108 pKa = 11.84 KK109 pKa = 8.31 RR110 pKa = 11.84 RR111 pKa = 11.84 RR112 pKa = 11.84 GPRR115 pKa = 11.84 RR116 pKa = 11.84 RR117 pKa = 11.84 GPRR120 pKa = 11.84 KK121 pKa = 9.31 RR122 pKa = 11.84 GLRR125 pKa = 11.84 RR126 pKa = 11.84 KK127 pKa = 8.8 GPKK130 pKa = 9.31 RR131 pKa = 11.84 RR132 pKa = 11.84 VQRR135 pKa = 11.84 RR136 pKa = 11.84 RR137 pKa = 11.84 GPRR140 pKa = 11.84 RR141 pKa = 11.84 KK142 pKa = 10.11 GPRR145 pKa = 11.84 KK146 pKa = 8.78 KK147 pKa = 9.84 RR148 pKa = 11.84 KK149 pKa = 8.92 RR150 pKa = 11.84 MVRR153 pKa = 11.84 RR154 pKa = 11.84 RR155 pKa = 11.84 KK156 pKa = 8.75 LQRR159 pKa = 11.84 RR160 pKa = 11.84 SKK162 pKa = 9.5 VSKK165 pKa = 9.78 RR166 pKa = 11.84 GPRR169 pKa = 11.84 RR170 pKa = 11.84 KK171 pKa = 9.25 RR172 pKa = 11.84 RR173 pKa = 11.84 RR174 pKa = 11.84 RR175 pKa = 11.84 RR176 pKa = 11.84 EE177 pKa = 3.14 RR178 pKa = 11.84 RR179 pKa = 11.84 RR180 pKa = 11.84 RR181 pKa = 11.84 RR182 pKa = 11.84 KK183 pKa = 9.36 RR184 pKa = 11.84 RR185 pKa = 11.84 RR186 pKa = 11.84 AGRR189 pKa = 11.84 KK190 pKa = 7.51 KK191 pKa = 10.22 RR192 pKa = 11.84 KK193 pKa = 9.35 RR194 pKa = 11.84 SRR196 pKa = 11.84 KK197 pKa = 9.2 KK198 pKa = 9.46 KK199 pKa = 9.57 RR200 pKa = 11.84 KK201 pKa = 9.3 RR202 pKa = 11.84 NLKK205 pKa = 9.65 RR206 pKa = 11.84 KK207 pKa = 9.52 RR208 pKa = 11.84 SRR210 pKa = 11.84 KK211 pKa = 8.64 RR212 pKa = 11.84 KK213 pKa = 8.77 RR214 pKa = 11.84 NRR216 pKa = 11.84 KK217 pKa = 8.53 RR218 pKa = 11.84 KK219 pKa = 8.97 RR220 pKa = 11.84 SRR222 pKa = 11.84 KK223 pKa = 8.09 RR224 pKa = 11.84 RR225 pKa = 11.84 KK226 pKa = 8.79 KK227 pKa = 8.74 RR228 pKa = 11.84 RR229 pKa = 11.84 RR230 pKa = 11.84 RR231 pKa = 11.84 KK232 pKa = 8.39 GRR234 pKa = 11.84 KK235 pKa = 7.1 RR236 pKa = 11.84 KK237 pKa = 8.99 KK238 pKa = 8.86 RR239 pKa = 11.84 RR240 pKa = 11.84 RR241 pKa = 11.84 GRR243 pKa = 11.84 RR244 pKa = 11.84 KK245 pKa = 8.64 RR246 pKa = 11.84 RR247 pKa = 11.84 RR248 pKa = 11.84 RR249 pKa = 11.84 RR250 pKa = 11.84 LRR252 pKa = 11.84 KK253 pKa = 9.02 RR254 pKa = 11.84 KK255 pKa = 9.21 RR256 pKa = 11.84 KK257 pKa = 9.22 RR258 pKa = 11.84 RR259 pKa = 11.84 RR260 pKa = 11.84 KK261 pKa = 9.08 KK262 pKa = 8.14 RR263 pKa = 11.84 RR264 pKa = 11.84 KK265 pKa = 8.22 RR266 pKa = 11.84 RR267 pKa = 11.84 RR268 pKa = 11.84 RR269 pKa = 11.84 RR270 pKa = 11.84 KK271 pKa = 8.04 GRR273 pKa = 11.84 RR274 pKa = 11.84 RR275 pKa = 11.84 KK276 pKa = 9.1 GRR278 pKa = 11.84 KK279 pKa = 8.36 RR280 pKa = 11.84 SRR282 pKa = 11.84 KK283 pKa = 8.29 RR284 pKa = 11.84 RR285 pKa = 11.84 KK286 pKa = 9.07 KK287 pKa = 8.29 RR288 pKa = 11.84 RR289 pKa = 11.84 KK290 pKa = 8.04 RR291 pKa = 11.84 RR292 pKa = 11.84 RR293 pKa = 11.84 RR294 pKa = 11.84 GKK296 pKa = 8.58 RR297 pKa = 11.84 RR298 pKa = 11.84 RR299 pKa = 11.84 RR300 pKa = 11.84 RR301 pKa = 11.84 RR302 pKa = 11.84 LIRR305 pKa = 11.84 TKK307 pKa = 10.93 KK308 pKa = 8.81 MRR310 pKa = 11.84 AKK312 pKa = 10.24 VKK314 pKa = 9.2 VKK316 pKa = 10.47 KK317 pKa = 10.3 GVRR320 pKa = 11.84 SCGQEE325 pKa = 3.88 PEE327 pKa = 4.31 GTGCNGWKK335 pKa = 10.16 KK336 pKa = 10.42 GIQRR340 pKa = 11.84 KK341 pKa = 8.44 RR342 pKa = 11.84 AHH344 pKa = 6.19 CTDD347 pKa = 5.58 LIAVANIPLIIILEE361 pKa = 4.05 KK362 pKa = 9.66 MKK364 pKa = 10.67 RR365 pKa = 11.84 VLKK368 pKa = 10.42 LAIIQCIPTHH378 pKa = 6.85 AYY380 pKa = 9.67 AA381 pKa = 5.4
Molecular weight: 49.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.301
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.691
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.398
IPC_peptide 13.495
IPC2_peptide 12.486
IPC2.peptide.svr19 9.187
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26895
0
26895
4856716
29
6632
180.6
20.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.519 ± 0.021
2.252 ± 0.013
5.258 ± 0.019
6.545 ± 0.022
4.484 ± 0.015
5.473 ± 0.026
2.37 ± 0.009
5.426 ± 0.013
5.592 ± 0.017
9.308 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.626 ± 0.01
4.126 ± 0.012
4.703 ± 0.023
4.168 ± 0.017
6.108 ± 0.016
7.952 ± 0.022
5.317 ± 0.017
6.484 ± 0.016
1.114 ± 0.006
3.03 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here