Lettuce infectious yellows virus (isolate United States/92) (LIYV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Crinivirus; Lettuce infectious yellows virus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q83044|R1A_LIYV9 Replicase polyprotein 1a OS=Lettuce infectious yellows virus (isolate United States/92) OX=651355 GN=ORF1a PE=4 SV=1
MM1 pKa = 7.49DD2 pKa = 4.51TKK4 pKa = 10.85TLIDD8 pKa = 4.14KK9 pKa = 10.8YY10 pKa = 10.84NIEE13 pKa = 4.23NFTNYY18 pKa = 9.79INFIIRR24 pKa = 11.84NHH26 pKa = 5.21QAGKK30 pKa = 10.19GNLRR34 pKa = 11.84FLVNLLKK41 pKa = 8.82TTGGSNLKK49 pKa = 10.01EE50 pKa = 3.66LDD52 pKa = 3.52INPVEE57 pKa = 4.22IEE59 pKa = 4.14NFNIDD64 pKa = 2.87IYY66 pKa = 11.36LDD68 pKa = 3.65FLEE71 pKa = 5.24FCLDD75 pKa = 3.47SKK77 pKa = 11.04FIFF80 pKa = 4.34

Molecular weight: 9.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q83047|MVP_LIYV9 Movement protein Hsp70h OS=Lettuce infectious yellows virus (isolate United States/92) OX=651355 PE=3 SV=1
MM1 pKa = 8.83IMMSPLYY8 pKa = 10.42ALTKK12 pKa = 10.14QCVIDD17 pKa = 3.38TAYY20 pKa = 10.86RR21 pKa = 11.84LAVPTQHH28 pKa = 6.55CAIYY32 pKa = 9.48TVACRR37 pKa = 11.84ILFLSVGFMTIVKK50 pKa = 10.11LCGFKK55 pKa = 9.87MDD57 pKa = 3.64TSSFIASIEE66 pKa = 3.92KK67 pKa = 10.49DD68 pKa = 3.31NLMDD72 pKa = 4.2CLISLVEE79 pKa = 3.81MRR81 pKa = 11.84DD82 pKa = 3.43RR83 pKa = 11.84LRR85 pKa = 11.84LCNDD89 pKa = 3.77FPILNYY95 pKa = 10.18GVNILEE101 pKa = 4.11LLIGKK106 pKa = 9.14RR107 pKa = 11.84LNKK110 pKa = 9.83INNLKK115 pKa = 8.6NCYY118 pKa = 9.57VIRR121 pKa = 11.84EE122 pKa = 4.74LITINISKK130 pKa = 9.91EE131 pKa = 4.12WVGKK135 pKa = 9.32QALKK139 pKa = 10.99VGLHH143 pKa = 6.26CFLNLSQADD152 pKa = 3.9SRR154 pKa = 11.84HH155 pKa = 4.35VKK157 pKa = 10.47YY158 pKa = 10.76LLSDD162 pKa = 3.66KK163 pKa = 11.02EE164 pKa = 4.68SLNKK168 pKa = 9.83MNFSRR173 pKa = 11.84YY174 pKa = 8.42YY175 pKa = 8.19VPKK178 pKa = 10.62VVTDD182 pKa = 4.5LYY184 pKa = 11.24LDD186 pKa = 4.97LIGVLYY192 pKa = 11.18VNTGYY197 pKa = 11.09NIDD200 pKa = 3.95LVEE203 pKa = 4.61KK204 pKa = 10.31FIFDD208 pKa = 3.39KK209 pKa = 11.62LEE211 pKa = 3.7FLVYY215 pKa = 10.57DD216 pKa = 3.73GEE218 pKa = 4.56EE219 pKa = 4.57GFKK222 pKa = 10.8SPQVEE227 pKa = 4.27YY228 pKa = 11.58NDD230 pKa = 3.02ICTVNNLKK238 pKa = 10.37PIIKK242 pKa = 9.4YY243 pKa = 9.71NRR245 pKa = 11.84WHH247 pKa = 6.6TDD249 pKa = 2.56GSIVIEE255 pKa = 4.58CGDD258 pKa = 3.97VIGKK262 pKa = 9.42GINKK266 pKa = 7.65TKK268 pKa = 10.63KK269 pKa = 9.92KK270 pKa = 10.24FAINDD275 pKa = 3.4AKK277 pKa = 11.37AEE279 pKa = 4.01FVKK282 pKa = 10.79NFKK285 pKa = 10.59AKK287 pKa = 10.46NKK289 pKa = 10.1NNEE292 pKa = 3.68

Molecular weight: 33.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

6620

80

2379

735.6

84.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.063 ± 0.277

2.13 ± 0.239

6.616 ± 0.353

5.755 ± 0.37

5.332 ± 0.157

4.562 ± 0.25

1.813 ± 0.207

7.764 ± 0.346

8.52 ± 0.266

8.867 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.085 ± 0.116

6.737 ± 0.433

2.931 ± 0.182

2.356 ± 0.181

5.151 ± 0.341

7.689 ± 0.283

5.483 ± 0.185

6.36 ± 0.334

0.967 ± 0.161

4.819 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski