Coraliomargarita sinensis
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2981 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A317ZDZ7|A0A317ZDZ7_9BACT Putative pre-16S rRNA nuclease OS=Coraliomargarita sinensis OX=2174842 GN=DDZ13_14855 PE=3 SV=1
MM1 pKa = 7.2 QKK3 pKa = 10.13 NAPICLALAVCYY15 pKa = 7.37 LTWLAPLRR23 pKa = 11.84 AADD26 pKa = 3.42 ITFVGHH32 pKa = 5.82 QDD34 pKa = 3.35 AVEE37 pKa = 4.37 ADD39 pKa = 3.51 ADD41 pKa = 4.27 TPTSGWRR48 pKa = 11.84 NATPSKK54 pKa = 9.89 TLDD57 pKa = 3.16 IDD59 pKa = 3.51 VDD61 pKa = 3.99 NVLGTDD67 pKa = 4.73 GYY69 pKa = 11.13 QVAAKK74 pKa = 9.73 SANPSYY80 pKa = 10.92 GWIIRR85 pKa = 11.84 RR86 pKa = 11.84 SPGYY90 pKa = 10.03 NHH92 pKa = 7.14 ASSWGLWDD100 pKa = 5.44 DD101 pKa = 4.16 PDD103 pKa = 3.92 NASGSDD109 pKa = 3.48 NLSAGVFYY117 pKa = 10.78 DD118 pKa = 4.04 PNANGQVDD126 pKa = 4.02 VFEE129 pKa = 4.76 LVIEE133 pKa = 4.29 GDD135 pKa = 3.74 EE136 pKa = 4.37 LVGKK140 pKa = 6.6 TLRR143 pKa = 11.84 VGILYY148 pKa = 10.38 GVMVNGTSTFTVTQTVGGNQMVTTPSLTHH177 pKa = 7.25 DD178 pKa = 3.57 ATSLNAAFFNITGAVEE194 pKa = 4.1 GDD196 pKa = 3.49 TFRR199 pKa = 11.84 ISTEE203 pKa = 3.47 ILTGSGVEE211 pKa = 3.93 QVGGIFLDD219 pKa = 3.91 SAPTNPGTQLPEE231 pKa = 3.28 VDD233 pKa = 3.24 GTVRR237 pKa = 11.84 GTYY240 pKa = 8.81 VAPLEE245 pKa = 4.26 VAGIFSDD252 pKa = 3.18 NGVIQRR258 pKa = 11.84 GMVVPVWGWDD268 pKa = 3.5 DD269 pKa = 4.02 PGTVVTVDD277 pKa = 3.8 FAGQSVNGTADD288 pKa = 3.23 ANGRR292 pKa = 11.84 WEE294 pKa = 4.63 AEE296 pKa = 4.16 LLSLTANTTPQTMTISAGADD316 pKa = 2.85 TVTISNLLVGDD327 pKa = 3.88 VWLCSGQSNMEE338 pKa = 3.65 MDD340 pKa = 4.43 FNGSQYY346 pKa = 10.97 AADD349 pKa = 3.94 MPAFIDD355 pKa = 3.81 PDD357 pKa = 3.5 NFPTIRR363 pKa = 11.84 HH364 pKa = 5.89 IDD366 pKa = 3.24 ISNAQAEE373 pKa = 4.65 FRR375 pKa = 11.84 QKK377 pKa = 10.76 RR378 pKa = 11.84 LPNGNAGWTVCDD390 pKa = 3.39 STTVQDD396 pKa = 3.46 YY397 pKa = 8.21 TAAGFFFATTISQEE411 pKa = 3.96 TGVPIGLINSTFGNSRR427 pKa = 11.84 IGEE430 pKa = 4.32 FVTPDD435 pKa = 3.45 SIGLVTDD442 pKa = 4.09 QIPASSFRR450 pKa = 11.84 GVFTPDD456 pKa = 2.85 PCKK459 pKa = 10.7 KK460 pKa = 9.44 FFAMVAPITGYY471 pKa = 11.74 AMTGALWYY479 pKa = 10.41 QGEE482 pKa = 4.24 ANANDD487 pKa = 3.81 GEE489 pKa = 4.55 DD490 pKa = 4.16 YY491 pKa = 10.36 FWKK494 pKa = 10.47 LAALFQGWRR503 pKa = 11.84 DD504 pKa = 3.1 EE505 pKa = 4.21 WQQGDD510 pKa = 3.92 FPFYY514 pKa = 10.21 YY515 pKa = 10.58 AQLPSFDD522 pKa = 5.1 GSVSWPIIRR531 pKa = 11.84 EE532 pKa = 4.08 SQRR535 pKa = 11.84 RR536 pKa = 11.84 ALTLTNTGMATLVDD550 pKa = 3.86 VGGVGLPWPEE560 pKa = 3.97 NLHH563 pKa = 6.45 PPNKK567 pKa = 9.44 YY568 pKa = 10.09 DD569 pKa = 3.13 VGRR572 pKa = 11.84 RR573 pKa = 11.84 LAQWALLNEE582 pKa = 4.39 YY583 pKa = 9.15 GQSSVVPSGPLFSSAVFDD601 pKa = 4.04 GANVTISFDD610 pKa = 3.77 YY611 pKa = 10.97 VGSGLVAAAKK621 pKa = 9.15 PSPQSTDD628 pKa = 2.91 APSPVADD635 pKa = 3.31 VLGFEE640 pKa = 4.79 LAGSDD645 pKa = 4.24 EE646 pKa = 4.33 VWHH649 pKa = 6.6 AANASIAGSTVVLSSASVPNPEE671 pKa = 3.97 AARR674 pKa = 11.84 YY675 pKa = 9.18 LYY677 pKa = 9.26 ATNTDD682 pKa = 3.44 GGTLYY687 pKa = 11.0 NLEE690 pKa = 5.11 DD691 pKa = 3.8 LPAAPFLTTAADD703 pKa = 3.9 NPNPPSTPTGLNATSGDD720 pKa = 3.48 GRR722 pKa = 11.84 VDD724 pKa = 4.15 LAWDD728 pKa = 3.71 AATGADD734 pKa = 3.58 SYY736 pKa = 11.26 NVKK739 pKa = 10.43 RR740 pKa = 11.84 STTTGGPYY748 pKa = 10.58 ASIGSPSGTSYY759 pKa = 12.01 ADD761 pKa = 3.15 TTAANGTTYY770 pKa = 10.38 YY771 pKa = 10.83 YY772 pKa = 11.13 VVAAVNTIGSSADD785 pKa = 3.36 STEE788 pKa = 4.21 VSATPVEE795 pKa = 4.52 ATNPPDD801 pKa = 3.85 APTGLSATPGDD812 pKa = 3.78 NRR814 pKa = 11.84 VDD816 pKa = 4.2 LSWSASSGADD826 pKa = 2.95 SYY828 pKa = 11.44 NVKK831 pKa = 10.36 RR832 pKa = 11.84 STTMGGPYY840 pKa = 9.81 SGIGTTPGISYY851 pKa = 10.13 TDD853 pKa = 3.84 LSATNGTTYY862 pKa = 10.69 YY863 pKa = 10.56 YY864 pKa = 10.83 VVSASNTYY872 pKa = 10.6 GNSADD877 pKa = 3.75 SAEE880 pKa = 4.4 ASATPQEE887 pKa = 4.32 PAAGAVITYY896 pKa = 9.72 VGHH899 pKa = 6.42 QDD901 pKa = 3.45 NVDD904 pKa = 3.5 AKK906 pKa = 10.12 TDD908 pKa = 3.72 DD909 pKa = 3.88 PTSGWRR915 pKa = 11.84 NTTPAKK921 pKa = 9.78 THH923 pKa = 7.22 DD924 pKa = 3.6 IDD926 pKa = 4.58 GDD928 pKa = 4.16 NILGTDD934 pKa = 4.47 GYY936 pKa = 11.88 AMFRR940 pKa = 11.84 NNSAFSVVSLPSYY953 pKa = 10.18 ISAVTHH959 pKa = 6.41 VSTHH963 pKa = 5.65 SNANASFGVMDD974 pKa = 4.63 NPSDD978 pKa = 3.75 PSGSDD983 pKa = 3.61 DD984 pKa = 3.25 INYY987 pKa = 9.25 GLWYY991 pKa = 9.02 LTTTGGTTDD1000 pKa = 3.18 VFTFTITGTDD1010 pKa = 3.44 LDD1012 pKa = 4.33 GKK1014 pKa = 6.76 TLRR1017 pKa = 11.84 VGLAYY1022 pKa = 10.3 DD1023 pKa = 4.88 GYY1025 pKa = 10.86 NGSGNQQLTITQTAGGAAADD1045 pKa = 3.83 NSGTVSWGNDD1055 pKa = 2.85 GLDD1058 pKa = 3.35 FVFFDD1063 pKa = 3.3 ITEE1066 pKa = 4.23 VNDD1069 pKa = 3.53 GDD1071 pKa = 4.26 VFTVNASAGAGLPHH1085 pKa = 6.46 VSGVTFDD1092 pKa = 4.32 VITDD1096 pKa = 3.62 IATPPGTPTGLAATPGDD1113 pKa = 3.9 GQVDD1117 pKa = 4.21 LTWNVSSGAASYY1129 pKa = 10.21 NVKK1132 pKa = 10.43 RR1133 pKa = 11.84 GTAEE1137 pKa = 4.01 GGPYY1141 pKa = 8.85 STVASPTTNSFSDD1154 pKa = 3.6 STVSNGTTYY1163 pKa = 10.73 YY1164 pKa = 10.58 YY1165 pKa = 10.9 VVSAVNAEE1173 pKa = 4.19 GEE1175 pKa = 4.5 SPDD1178 pKa = 3.8 SGEE1181 pKa = 5.04 ASATPQALPPDD1192 pKa = 4.27 APTGLVATPGDD1203 pKa = 3.99 GQVDD1207 pKa = 4.5 LSWDD1211 pKa = 3.46 ASSGADD1217 pKa = 3.08 SYY1219 pKa = 11.35 NVKK1222 pKa = 10.28 RR1223 pKa = 11.84 GTSMGGPYY1231 pKa = 9.0 STVASPNNNSLTDD1244 pKa = 3.54 ATVSNGTTYY1253 pKa = 10.73 YY1254 pKa = 10.58 YY1255 pKa = 10.86 VVSAVNTNGEE1265 pKa = 4.34 SGNSGEE1271 pKa = 5.07 VSATPQAAPTGATISYY1287 pKa = 9.94 VGNQTNVEE1295 pKa = 4.34 TEE1297 pKa = 3.78 ASDD1300 pKa = 5.22 AGIGWRR1306 pKa = 11.84 NTTPDD1311 pKa = 3.75 KK1312 pKa = 11.11 PLDD1315 pKa = 3.58 IDD1317 pKa = 3.95 GDD1319 pKa = 4.29 NILGTDD1325 pKa = 4.41 GYY1327 pKa = 11.14 RR1328 pKa = 11.84 IFNDD1332 pKa = 3.55 GSTVSLPSYY1341 pKa = 9.85 IDD1343 pKa = 4.33 NITKK1347 pKa = 10.22 IAPNAAKK1354 pKa = 10.44 SGGWGVIDD1362 pKa = 5.7 DD1363 pKa = 4.82 PSDD1366 pKa = 3.48 PSGTDD1371 pKa = 3.69 DD1372 pKa = 4.42 IIHH1375 pKa = 6.12 GFWYY1379 pKa = 10.08 DD1380 pKa = 3.07 SGNAQGSVDD1389 pKa = 3.26 ILSFQITGTALDD1401 pKa = 4.19 GQTLRR1406 pKa = 11.84 IGIIYY1411 pKa = 7.63 DD1412 pKa = 3.72 THH1414 pKa = 6.66 WGTGDD1419 pKa = 3.0 IAFTLTQNGTSGSVTATSGLVTAGSDD1445 pKa = 3.75 GIDD1448 pKa = 3.12 VVFFDD1453 pKa = 4.64 LTNVSSGDD1461 pKa = 3.76 VFTLNADD1468 pKa = 3.65 ADD1470 pKa = 4.0 DD1471 pKa = 5.09 HH1472 pKa = 6.38 NFSHH1476 pKa = 7.23 AGGITFDD1483 pKa = 3.52 TSVISGGDD1491 pKa = 3.43 TLADD1495 pKa = 3.2 WLASPEE1501 pKa = 4.26 FGLDD1505 pKa = 3.56 PADD1508 pKa = 3.94 QDD1510 pKa = 5.15 FGDD1513 pKa = 4.93 DD1514 pKa = 3.78 PDD1516 pKa = 6.15 GDD1518 pKa = 3.63 GHH1520 pKa = 7.71 ANGIEE1525 pKa = 4.41 NYY1527 pKa = 10.05 FGTHH1531 pKa = 6.45 PGEE1534 pKa = 4.34 FSQGLVSGTVSGDD1547 pKa = 2.96 SMTFTHH1553 pKa = 7.16 PLSDD1557 pKa = 3.96 SPAADD1562 pKa = 2.99 ISAVYY1567 pKa = 9.68 RR1568 pKa = 11.84 WSKK1571 pKa = 11.06 DD1572 pKa = 2.92 LDD1574 pKa = 3.88 SFHH1577 pKa = 8.1 VDD1579 pKa = 2.95 GDD1581 pKa = 4.17 SFDD1584 pKa = 3.73 NTTVTFSQGTPADD1597 pKa = 3.48 GMVEE1601 pKa = 3.82 VTAAITGDD1609 pKa = 3.87 PLDD1612 pKa = 4.14 KK1613 pKa = 10.91 LFVDD1617 pKa = 4.9 IEE1619 pKa = 4.45 VTQPP1623 pKa = 2.93
Molecular weight: 168.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.67
IPC2_protein 3.732
IPC_protein 3.808
Toseland 3.554
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.77
Rodwell 3.617
Grimsley 3.465
Solomon 3.808
Lehninger 3.77
Nozaki 3.91
DTASelect 4.228
Thurlkill 3.617
EMBOSS 3.77
Sillero 3.923
Patrickios 1.163
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A317ZD97|A0A317ZD97_9BACT Cytochrome oxidase assembly protein OS=Coraliomargarita sinensis OX=2174842 GN=DDZ13_13100 PE=4 SV=1
MM1 pKa = 7.59 GNLKK5 pKa = 10.05 KK6 pKa = 10.3 KK7 pKa = 10.32 RR8 pKa = 11.84 RR9 pKa = 11.84 LKK11 pKa = 9.51 MNKK14 pKa = 8.33 HH15 pKa = 5.49 KK16 pKa = 10.35 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.17 RR20 pKa = 11.84 LKK22 pKa = 9.94 SNRR25 pKa = 11.84 HH26 pKa = 5.57 KK27 pKa = 10.88 KK28 pKa = 8.44 RR29 pKa = 11.84 TWW31 pKa = 2.8
Molecular weight: 4.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.261
Rodwell 12.705
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.427
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.035
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2981
0
2981
1056829
15
5145
354.5
39.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.111 ± 0.049
1.019 ± 0.018
6.035 ± 0.055
7.15 ± 0.056
4.201 ± 0.03
7.778 ± 0.054
2.106 ± 0.028
5.691 ± 0.04
4.803 ± 0.066
9.752 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.18 ± 0.025
3.593 ± 0.036
4.712 ± 0.03
3.405 ± 0.028
5.716 ± 0.057
6.443 ± 0.051
5.301 ± 0.075
6.584 ± 0.04
1.405 ± 0.021
3.015 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here