Methanobrevibacter sp. NOE

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobrevibacter; unclassified Methanobrevibacter

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1736 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A366MB73|A0A366MB73_9EURY RNA-splicing ligase RtcB OS=Methanobrevibacter sp. NOE OX=2006182 GN=rtcB PE=3 SV=1
MM1 pKa = 7.32YY2 pKa = 9.91IINSSFSNSPSYY14 pKa = 10.77IGDD17 pKa = 3.51GGFSGHH23 pKa = 6.5GGAIFFNTNSKK34 pKa = 11.3SNISNSNFTNNIASEE49 pKa = 4.27GGGIFINNGDD59 pKa = 4.64DD60 pKa = 3.57LTINDD65 pKa = 5.34SIFSDD70 pKa = 3.17NFANVNGGAVFLNPNN85 pKa = 3.54

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A366MAR4|A0A366MAR4_9EURY Ribonuclease P protein component 2 OS=Methanobrevibacter sp. NOE OX=2006182 GN=rnp2 PE=3 SV=1
MM1 pKa = 7.04TVRR4 pKa = 11.84GVAMNAVDD12 pKa = 4.06HH13 pKa = 6.08PHH15 pKa = 6.84GGGNRR20 pKa = 11.84QHH22 pKa = 7.4PGRR25 pKa = 11.84PTTVSRR31 pKa = 11.84HH32 pKa = 4.6TPPGRR37 pKa = 11.84KK38 pKa = 9.1VGSIAAKK45 pKa = 8.74RR46 pKa = 11.84TGKK49 pKa = 10.07RR50 pKa = 11.84RR51 pKa = 3.34

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1736

0

1736

389240

29

2074

224.2

25.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.416 ± 0.059

1.149 ± 0.034

6.138 ± 0.052

7.341 ± 0.064

4.206 ± 0.051

6.53 ± 0.063

1.611 ± 0.023

10.247 ± 0.073

8.656 ± 0.072

8.488 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.029

7.209 ± 0.093

3.21 ± 0.038

2.158 ± 0.03

3.093 ± 0.043

6.593 ± 0.054

5.095 ± 0.047

6.232 ± 0.05

0.612 ± 0.019

3.441 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski