Amino acid dipepetide frequency for Methanobrevibacter sp. NOE

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.468AlaAla: 3.468 ± 0.113
0.678AlaCys: 0.678 ± 0.051
2.967AlaAsp: 2.967 ± 0.085
3.453AlaGlu: 3.453 ± 0.093
2.076AlaPhe: 2.076 ± 0.072
3.805AlaGly: 3.805 ± 0.113
0.897AlaHis: 0.897 ± 0.05
6.279AlaIle: 6.279 ± 0.172
4.871AlaLys: 4.871 ± 0.108
4.637AlaLeu: 4.637 ± 0.122
1.619AlaMet: 1.619 ± 0.069
3.276AlaAsn: 3.276 ± 0.094
1.583AlaPro: 1.583 ± 0.066
1.105AlaGln: 1.105 ± 0.053
1.701AlaArg: 1.701 ± 0.077
3.235AlaSer: 3.235 ± 0.084
3.001AlaThr: 3.001 ± 0.086
3.72AlaVal: 3.72 ± 0.116
0.239AlaTrp: 0.239 ± 0.023
1.557AlaTyr: 1.557 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.588CysAla: 0.588 ± 0.044
0.162CysCys: 0.162 ± 0.023
0.771CysAsp: 0.771 ± 0.055
0.853CysGlu: 0.853 ± 0.052
0.367CysPhe: 0.367 ± 0.036
1.3CysGly: 1.3 ± 0.078
0.221CysHis: 0.221 ± 0.024
0.938CysIle: 0.938 ± 0.07
0.686CysLys: 0.686 ± 0.049
0.776CysLeu: 0.776 ± 0.049
0.277CysMet: 0.277 ± 0.028
0.632CysAsn: 0.632 ± 0.037
0.814CysPro: 0.814 ± 0.079
0.272CysGln: 0.272 ± 0.025
0.378CysArg: 0.378 ± 0.033
0.719CysSer: 0.719 ± 0.039
0.555CysThr: 0.555 ± 0.046
0.778CysVal: 0.778 ± 0.061
0.057CysTrp: 0.057 ± 0.011
0.342CysTyr: 0.342 ± 0.034
0.0CysXaa: 0.0 ± 0.0
Asp
2.726AspAla: 2.726 ± 0.088
0.637AspCys: 0.637 ± 0.047
3.589AspAsp: 3.589 ± 0.103
5.213AspGlu: 5.213 ± 0.138
2.934AspPhe: 2.934 ± 0.075
3.396AspGly: 3.396 ± 0.095
0.943AspHis: 0.943 ± 0.052
6.919AspIle: 6.919 ± 0.161
4.946AspLys: 4.946 ± 0.113
5.996AspLeu: 5.996 ± 0.144
1.855AspMet: 1.855 ± 0.076
3.908AspAsn: 3.908 ± 0.11
2.217AspPro: 2.217 ± 0.079
1.179AspGln: 1.179 ± 0.058
1.559AspArg: 1.559 ± 0.076
3.951AspSer: 3.951 ± 0.106
2.492AspThr: 2.492 ± 0.08
4.162AspVal: 4.162 ± 0.12
0.432AspTrp: 0.432 ± 0.038
2.33AspTyr: 2.33 ± 0.074
0.0AspXaa: 0.0 ± 0.0
Glu
3.784GluAla: 3.784 ± 0.118
0.642GluCys: 0.642 ± 0.043
4.709GluAsp: 4.709 ± 0.128
6.531GluGlu: 6.531 ± 0.157
2.911GluPhe: 2.911 ± 0.092
3.964GluGly: 3.964 ± 0.094
0.966GluHis: 0.966 ± 0.05
8.188GluIle: 8.188 ± 0.17
7.79GluLys: 7.79 ± 0.145
6.287GluLeu: 6.287 ± 0.151
1.847GluMet: 1.847 ± 0.069
6.03GluAsn: 6.03 ± 0.132
1.603GluPro: 1.603 ± 0.073
1.182GluGln: 1.182 ± 0.063
2.155GluArg: 2.155 ± 0.078
3.964GluSer: 3.964 ± 0.108
3.527GluThr: 3.527 ± 0.102
4.373GluVal: 4.373 ± 0.119
0.388GluTrp: 0.388 ± 0.033
2.574GluTyr: 2.574 ± 0.08
0.0GluXaa: 0.0 ± 0.0
Phe
2.037PheAla: 2.037 ± 0.068
0.421PheCys: 0.421 ± 0.031
2.921PheAsp: 2.921 ± 0.082
3.157PheGlu: 3.157 ± 0.091
1.652PhePhe: 1.652 ± 0.076
2.741PheGly: 2.741 ± 0.096
0.709PheHis: 0.709 ± 0.042
4.139PheIle: 4.139 ± 0.109
3.499PheLys: 3.499 ± 0.098
3.751PheLeu: 3.751 ± 0.117
0.992PheMet: 0.992 ± 0.056
3.065PheAsn: 3.065 ± 0.116
1.285PhePro: 1.285 ± 0.064
0.879PheGln: 0.879 ± 0.048
1.243PheArg: 1.243 ± 0.059
3.103PheSer: 3.103 ± 0.098
2.145PheThr: 2.145 ± 0.087
2.59PheVal: 2.59 ± 0.09
0.224PheTrp: 0.224 ± 0.03
1.511PheTyr: 1.511 ± 0.075
0.0PheXaa: 0.0 ± 0.0
Gly
4.221GlyAla: 4.221 ± 0.127
0.922GlyCys: 0.922 ± 0.059
3.669GlyAsp: 3.669 ± 0.125
4.057GlyGlu: 4.057 ± 0.103
2.993GlyPhe: 2.993 ± 0.091
4.619GlyGly: 4.619 ± 0.147
1.156GlyHis: 1.156 ± 0.06
7.057GlyIle: 7.057 ± 0.159
5.231GlyLys: 5.231 ± 0.112
5.179GlyLeu: 5.179 ± 0.113
1.711GlyMet: 1.711 ± 0.069
3.843GlyAsn: 3.843 ± 0.128
1.744GlyPro: 1.744 ± 0.075
1.493GlyGln: 1.493 ± 0.066
2.094GlyArg: 2.094 ± 0.075
4.29GlySer: 4.29 ± 0.121
3.666GlyThr: 3.666 ± 0.11
4.524GlyVal: 4.524 ± 0.132
0.462GlyTrp: 0.462 ± 0.038
2.366GlyTyr: 2.366 ± 0.075
0.0GlyXaa: 0.0 ± 0.0
His
0.814HisAla: 0.814 ± 0.047
0.267HisCys: 0.267 ± 0.024
1.115HisAsp: 1.115 ± 0.061
1.125HisGlu: 1.125 ± 0.055
0.642HisPhe: 0.642 ± 0.037
1.246HisGly: 1.246 ± 0.062
0.355HisHis: 0.355 ± 0.034
1.428HisIle: 1.428 ± 0.057
0.951HisLys: 0.951 ± 0.054
1.434HisLeu: 1.434 ± 0.058
0.419HisMet: 0.419 ± 0.029
0.886HisAsn: 0.886 ± 0.053
0.799HisPro: 0.799 ± 0.043
0.393HisGln: 0.393 ± 0.032
0.596HisArg: 0.596 ± 0.039
0.974HisSer: 0.974 ± 0.053
0.814HisThr: 0.814 ± 0.047
1.11HisVal: 1.11 ± 0.055
0.108HisTrp: 0.108 ± 0.016
0.632HisTyr: 0.632 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
6.402IleAla: 6.402 ± 0.142
1.074IleCys: 1.074 ± 0.054
6.605IleAsp: 6.605 ± 0.143
7.438IleGlu: 7.438 ± 0.16
4.319IlePhe: 4.319 ± 0.113
6.811IleGly: 6.811 ± 0.152
1.688IleHis: 1.688 ± 0.064
10.115IleIle: 10.115 ± 0.187
8.239IleLys: 8.239 ± 0.191
9.018IleLeu: 9.018 ± 0.202
2.436IleMet: 2.436 ± 0.084
7.206IleAsn: 7.206 ± 0.187
3.63IlePro: 3.63 ± 0.091
2.058IleGln: 2.058 ± 0.06
2.931IleArg: 2.931 ± 0.094
7.086IleSer: 7.086 ± 0.14
5.154IleThr: 5.154 ± 0.13
6.384IleVal: 6.384 ± 0.115
0.547IleTrp: 0.547 ± 0.033
3.327IleTyr: 3.327 ± 0.103
0.0IleXaa: 0.0 ± 0.0
Lys
4.118LysAla: 4.118 ± 0.105
0.909LysCys: 0.909 ± 0.07
5.398LysAsp: 5.398 ± 0.123
7.579LysGlu: 7.579 ± 0.17
3.191LysPhe: 3.191 ± 0.118
4.432LysGly: 4.432 ± 0.106
1.3LysHis: 1.3 ± 0.061
9.323LysIle: 9.323 ± 0.171
8.553LysLys: 8.553 ± 0.183
6.677LysLeu: 6.677 ± 0.127
2.353LysMet: 2.353 ± 0.083
7.183LysAsn: 7.183 ± 0.182
2.307LysPro: 2.307 ± 0.076
1.922LysGln: 1.922 ± 0.064
2.749LysArg: 2.749 ± 0.099
5.192LysSer: 5.192 ± 0.121
4.745LysThr: 4.745 ± 0.117
4.75LysVal: 4.75 ± 0.109
0.522LysTrp: 0.522 ± 0.036
3.358LysTyr: 3.358 ± 0.091
0.0LysXaa: 0.0 ± 0.0
Leu
5.601LeuAla: 5.601 ± 0.15
0.789LeuCys: 0.789 ± 0.061
5.719LeuAsp: 5.719 ± 0.118
6.246LeuGlu: 6.246 ± 0.167
3.473LeuPhe: 3.473 ± 0.101
5.524LeuGly: 5.524 ± 0.126
1.133LeuHis: 1.133 ± 0.059
8.147LeuIle: 8.147 ± 0.167
7.969LeuLys: 7.969 ± 0.143
6.916LeuLeu: 6.916 ± 0.178
2.335LeuMet: 2.335 ± 0.079
6.181LeuAsn: 6.181 ± 0.138
2.687LeuPro: 2.687 ± 0.089
1.577LeuGln: 1.577 ± 0.067
2.6LeuArg: 2.6 ± 0.087
5.832LeuSer: 5.832 ± 0.149
4.326LeuThr: 4.326 ± 0.11
4.964LeuVal: 4.964 ± 0.113
0.486LeuTrp: 0.486 ± 0.035
2.369LeuTyr: 2.369 ± 0.079
0.0LeuXaa: 0.0 ± 0.0
Met
1.405MetAla: 1.405 ± 0.061
0.267MetCys: 0.267 ± 0.029
1.937MetAsp: 1.937 ± 0.076
1.822MetGlu: 1.822 ± 0.064
0.917MetPhe: 0.917 ± 0.046
1.963MetGly: 1.963 ± 0.074
0.393MetHis: 0.393 ± 0.027
2.561MetIle: 2.561 ± 0.08
2.549MetLys: 2.549 ± 0.078
1.852MetLeu: 1.852 ± 0.074
0.619MetMet: 0.619 ± 0.041
1.757MetAsn: 1.757 ± 0.071
0.768MetPro: 0.768 ± 0.044
0.473MetGln: 0.473 ± 0.037
0.866MetArg: 0.866 ± 0.049
1.665MetSer: 1.665 ± 0.062
1.305MetThr: 1.305 ± 0.062
1.804MetVal: 1.804 ± 0.064
0.146MetTrp: 0.146 ± 0.019
0.668MetTyr: 0.668 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
2.787AsnAla: 2.787 ± 0.089
0.822AsnCys: 0.822 ± 0.051
3.933AsnAsp: 3.933 ± 0.118
4.771AsnGlu: 4.771 ± 0.121
2.947AsnPhe: 2.947 ± 0.084
4.098AsnGly: 4.098 ± 0.133
1.056AsnHis: 1.056 ± 0.057
8.034AsnIle: 8.034 ± 0.19
6.197AsnLys: 6.197 ± 0.18
5.96AsnLeu: 5.96 ± 0.14
1.69AsnMet: 1.69 ± 0.074
7.592AsnAsn: 7.592 ± 0.281
2.554AsnPro: 2.554 ± 0.083
1.703AsnGln: 1.703 ± 0.077
1.901AsnArg: 1.901 ± 0.077
5.349AsnSer: 5.349 ± 0.178
3.417AsnThr: 3.417 ± 0.123
4.093AsnVal: 4.093 ± 0.114
0.514AsnTrp: 0.514 ± 0.044
2.677AsnTyr: 2.677 ± 0.106
0.0AsnXaa: 0.0 ± 0.0
Pro
1.534ProAla: 1.534 ± 0.07
0.403ProCys: 0.403 ± 0.034
1.824ProAsp: 1.824 ± 0.08
2.741ProGlu: 2.741 ± 0.094
1.426ProPhe: 1.426 ± 0.064
2.233ProGly: 2.233 ± 0.088
0.65ProHis: 0.65 ± 0.051
3.075ProIle: 3.075 ± 0.102
2.477ProLys: 2.477 ± 0.093
2.703ProLeu: 2.703 ± 0.096
0.748ProMet: 0.748 ± 0.043
1.976ProAsn: 1.976 ± 0.083
0.943ProPro: 0.943 ± 0.048
0.866ProGln: 0.866 ± 0.049
1.002ProArg: 1.002 ± 0.058
2.158ProSer: 2.158 ± 0.075
1.716ProThr: 1.716 ± 0.068
2.32ProVal: 2.32 ± 0.083
0.208ProTrp: 0.208 ± 0.024
1.102ProTyr: 1.102 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
1.197GlnAla: 1.197 ± 0.05
0.254GlnCys: 0.254 ± 0.024
1.092GlnAsp: 1.092 ± 0.048
1.521GlnGlu: 1.521 ± 0.073
0.866GlnPhe: 0.866 ± 0.049
1.236GlnGly: 1.236 ± 0.059
0.373GlnHis: 0.373 ± 0.032
2.084GlnIle: 2.084 ± 0.081
2.155GlnLys: 2.155 ± 0.078
1.806GlnLeu: 1.806 ± 0.065
0.714GlnMet: 0.714 ± 0.048
1.603GlnAsn: 1.603 ± 0.066
0.506GlnPro: 0.506 ± 0.033
0.581GlnGln: 0.581 ± 0.048
0.84GlnArg: 0.84 ± 0.051
1.285GlnSer: 1.285 ± 0.054
1.151GlnThr: 1.151 ± 0.053
1.218GlnVal: 1.218 ± 0.051
0.193GlnTrp: 0.193 ± 0.02
0.904GlnTyr: 0.904 ± 0.052
0.0GlnXaa: 0.0 ± 0.0
Arg
1.809ArgAla: 1.809 ± 0.069
0.424ArgCys: 0.424 ± 0.037
1.778ArgAsp: 1.778 ± 0.067
2.392ArgGlu: 2.392 ± 0.082
1.4ArgPhe: 1.4 ± 0.056
2.145ArgGly: 2.145 ± 0.076
0.501ArgHis: 0.501 ± 0.037
2.908ArgIle: 2.908 ± 0.082
2.726ArgLys: 2.726 ± 0.08
2.487ArgLeu: 2.487 ± 0.077
0.902ArgMet: 0.902 ± 0.047
1.819ArgAsn: 1.819 ± 0.061
1.01ArgPro: 1.01 ± 0.062
0.74ArgGln: 0.74 ± 0.048
1.31ArgArg: 1.31 ± 0.061
1.76ArgSer: 1.76 ± 0.07
1.631ArgThr: 1.631 ± 0.07
1.947ArgVal: 1.947 ± 0.077
0.185ArgTrp: 0.185 ± 0.022
1.056ArgTyr: 1.056 ± 0.059
0.0ArgXaa: 0.0 ± 0.0
Ser
3.265SerAla: 3.265 ± 0.094
0.686SerCys: 0.686 ± 0.054
3.733SerAsp: 3.733 ± 0.101
4.283SerGlu: 4.283 ± 0.104
3.155SerPhe: 3.155 ± 0.085
4.581SerGly: 4.581 ± 0.121
1.174SerHis: 1.174 ± 0.059
6.292SerIle: 6.292 ± 0.138
5.734SerLys: 5.734 ± 0.137
5.685SerLeu: 5.685 ± 0.121
1.526SerMet: 1.526 ± 0.066
4.717SerAsn: 4.717 ± 0.138
2.084SerPro: 2.084 ± 0.062
1.634SerGln: 1.634 ± 0.065
2.122SerArg: 2.122 ± 0.077
5.092SerSer: 5.092 ± 0.146
3.502SerThr: 3.502 ± 0.099
3.8SerVal: 3.8 ± 0.105
0.455SerTrp: 0.455 ± 0.033
2.412SerTyr: 2.412 ± 0.092
0.0SerXaa: 0.0 ± 0.0
Thr
2.813ThrAla: 2.813 ± 0.101
0.683ThrCys: 0.683 ± 0.048
3.014ThrAsp: 3.014 ± 0.1
2.975ThrGlu: 2.975 ± 0.093
2.22ThrPhe: 2.22 ± 0.078
3.931ThrGly: 3.931 ± 0.112
0.92ThrHis: 0.92 ± 0.05
5.552ThrIle: 5.552 ± 0.148
3.579ThrLys: 3.579 ± 0.101
4.493ThrLeu: 4.493 ± 0.108
1.187ThrMet: 1.187 ± 0.054
3.386ThrAsn: 3.386 ± 0.103
2.001ThrPro: 2.001 ± 0.073
1.205ThrGln: 1.205 ± 0.054
1.647ThrArg: 1.647 ± 0.065
3.245ThrSer: 3.245 ± 0.089
2.901ThrThr: 2.901 ± 0.093
3.268ThrVal: 3.268 ± 0.094
0.272ThrTrp: 0.272 ± 0.025
1.66ThrTyr: 1.66 ± 0.069
0.0ThrXaa: 0.0 ± 0.0
Val
3.795ValAla: 3.795 ± 0.122
0.843ValCys: 0.843 ± 0.053
4.167ValAsp: 4.167 ± 0.1
4.47ValGlu: 4.47 ± 0.104
2.674ValPhe: 2.674 ± 0.08
4.229ValGly: 4.229 ± 0.128
0.953ValHis: 0.953 ± 0.05
5.765ValIle: 5.765 ± 0.129
5.21ValLys: 5.21 ± 0.12
5.562ValLeu: 5.562 ± 0.118
1.467ValMet: 1.467 ± 0.057
3.918ValAsn: 3.918 ± 0.116
2.176ValPro: 2.176 ± 0.079
1.313ValGln: 1.313 ± 0.07
1.893ValArg: 1.893 ± 0.076
4.293ValSer: 4.293 ± 0.116
2.913ValThr: 2.913 ± 0.085
4.37ValVal: 4.37 ± 0.136
0.326ValTrp: 0.326 ± 0.033
1.983ValTyr: 1.983 ± 0.071
0.0ValXaa: 0.0 ± 0.0
Trp
0.306TrpAla: 0.306 ± 0.032
0.08TrpCys: 0.08 ± 0.014
0.403TrpAsp: 0.403 ± 0.032
0.442TrpGlu: 0.442 ± 0.035
0.254TrpPhe: 0.254 ± 0.023
0.437TrpGly: 0.437 ± 0.036
0.072TrpHis: 0.072 ± 0.013
0.619TrpIle: 0.619 ± 0.046
0.524TrpLys: 0.524 ± 0.034
0.532TrpLeu: 0.532 ± 0.036
0.195TrpMet: 0.195 ± 0.025
0.437TrpAsn: 0.437 ± 0.035
0.177TrpPro: 0.177 ± 0.025
0.121TrpGln: 0.121 ± 0.02
0.226TrpArg: 0.226 ± 0.024
0.313TrpSer: 0.313 ± 0.032
0.295TrpThr: 0.295 ± 0.028
0.352TrpVal: 0.352 ± 0.034
0.103TrpTrp: 0.103 ± 0.019
0.231TrpTyr: 0.231 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.493TyrAla: 1.493 ± 0.065
0.522TyrCys: 0.522 ± 0.043
2.04TyrAsp: 2.04 ± 0.073
2.348TyrGlu: 2.348 ± 0.067
1.647TyrPhe: 1.647 ± 0.061
2.61TyrGly: 2.61 ± 0.091
0.65TyrHis: 0.65 ± 0.043
3.044TyrIle: 3.044 ± 0.094
2.677TyrLys: 2.677 ± 0.086
3.124TyrLeu: 3.124 ± 0.088
0.843TyrMet: 0.843 ± 0.048
2.68TyrAsn: 2.68 ± 0.1
1.215TyrPro: 1.215 ± 0.054
0.884TyrGln: 0.884 ± 0.047
1.115TyrArg: 1.115 ± 0.052
2.425TyrSer: 2.425 ± 0.08
1.693TyrThr: 1.693 ± 0.062
1.796TyrVal: 1.796 ± 0.069
0.254TyrTrp: 0.254 ± 0.024
1.351TyrTyr: 1.351 ± 0.067
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1736 proteins (389240 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski