Microlunatus sp. Gsoil 973

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Microlunatus; unclassified Microlunatus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I5VNC4|A0A6I5VNC4_9ACTN DUF4097 family beta strand repeat protein OS=Microlunatus sp. Gsoil 973 OX=2672569 GN=GJV80_19935 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 5.73SIATASFTPPPRR14 pKa = 11.84AGSYY18 pKa = 7.11YY19 pKa = 9.62WRR21 pKa = 11.84VSYY24 pKa = 11.04SGDD27 pKa = 3.57DD28 pKa = 3.51NYY30 pKa = 11.28TALPQSPCGEE40 pKa = 4.25AGEE43 pKa = 4.61TSTVTPAVPTIATQATTATLPNASVSDD70 pKa = 3.79TATVTGIPDD79 pKa = 3.43APIPTGTVTFRR90 pKa = 11.84LYY92 pKa = 10.59GASGTPVCTGDD103 pKa = 3.71PAFGPATVPLTDD115 pKa = 3.72GTATSPEE122 pKa = 4.11FSPTVAGDD130 pKa = 3.61YY131 pKa = 10.26FWLVDD136 pKa = 3.37YY137 pKa = 11.41SGDD140 pKa = 3.55ANYY143 pKa = 10.71EE144 pKa = 4.27PVTSTCDD151 pKa = 3.44DD152 pKa = 4.12PNEE155 pKa = 4.0KK156 pKa = 10.55SRR158 pKa = 11.84ITKK161 pKa = 10.25SAVLLTSSAADD172 pKa = 3.16ARR174 pKa = 11.84FGDD177 pKa = 4.58EE178 pKa = 4.66ISDD181 pKa = 3.5TGLLVGVGDD190 pKa = 3.95VAPTGTVTFTAYY202 pKa = 10.47GPTDD206 pKa = 3.31SDD208 pKa = 3.69EE209 pKa = 4.4PVCEE213 pKa = 4.24DD214 pKa = 3.89VAFAQAGTDD223 pKa = 3.66LQTTSPGTATADD235 pKa = 3.34ASFTPSDD242 pKa = 3.36PGRR245 pKa = 11.84YY246 pKa = 7.42FWRR249 pKa = 11.84ISYY252 pKa = 10.53SGDD255 pKa = 3.1SNYY258 pKa = 9.99VDD260 pKa = 3.54TVSDD264 pKa = 3.88CGALDD269 pKa = 3.45EE270 pKa = 4.58EE271 pKa = 4.99SRR273 pKa = 11.84VTVPSIDD280 pKa = 3.27IAKK283 pKa = 8.47TATPDD288 pKa = 3.32SFGSADD294 pKa = 3.93SPITYY299 pKa = 10.13NYY301 pKa = 10.44RR302 pKa = 11.84VTNTGDD308 pKa = 3.15ATLDD312 pKa = 3.49NVRR315 pKa = 11.84VTDD318 pKa = 3.63PHH320 pKa = 7.25DD321 pKa = 4.1GLSEE325 pKa = 4.03LACAPAQGSSLDD337 pKa = 3.84PGDD340 pKa = 4.64VMTCTAVYY348 pKa = 8.28ATTQADD354 pKa = 4.11LNAGSITNTATVTGTPPTGADD375 pKa = 3.35VTDD378 pKa = 3.47TDD380 pKa = 3.98QAVVTANQGPDD391 pKa = 2.83IQIAKK396 pKa = 9.15NASPGSFGAAGTPITYY412 pKa = 10.05SYY414 pKa = 11.32QVTNTGNVTLDD425 pKa = 3.7DD426 pKa = 3.87VRR428 pKa = 11.84VTDD431 pKa = 3.47PHH433 pKa = 7.47VGLSAVSCAPAQGSSLDD450 pKa = 3.84PGDD453 pKa = 4.64VMTCTAVYY461 pKa = 7.42TTTQADD467 pKa = 4.07LNAGSITNTATVTGTPPTGADD488 pKa = 3.35VTDD491 pKa = 3.47TDD493 pKa = 3.98QAVVTANQGPDD504 pKa = 2.83IQIAKK509 pKa = 9.15NASPGSFGAAGTPITYY525 pKa = 10.05SYY527 pKa = 11.32QVTNTGNVTLNNVRR541 pKa = 11.84VTDD544 pKa = 3.82PHH546 pKa = 7.44DD547 pKa = 4.11GLSDD551 pKa = 3.93LACAPNQGTSLDD563 pKa = 4.05PGDD566 pKa = 4.83VMTCTAVYY574 pKa = 7.57TTTQDD579 pKa = 3.54DD580 pKa = 4.43LNAGSITDD588 pKa = 3.61TATATGVSVAGDD600 pKa = 3.46NVLDD604 pKa = 3.85SDD606 pKa = 4.28EE607 pKa = 4.9ALVTASTAPAIDD619 pKa = 3.48IQKK622 pKa = 8.59TADD625 pKa = 3.34PTEE628 pKa = 4.56FGTAGTPITYY638 pKa = 8.82TYY640 pKa = 11.11QVTNTGNVTLDD651 pKa = 3.7DD652 pKa = 3.87VRR654 pKa = 11.84VTDD657 pKa = 3.47PHH659 pKa = 7.47VGLSAVSCAPAQGATLEE676 pKa = 5.19PGDD679 pKa = 4.98QMDD682 pKa = 3.92CTAVYY687 pKa = 7.57TTTQGDD693 pKa = 4.24LNSGSVTDD701 pKa = 3.6TATVVGSPPTGSNVTDD717 pKa = 3.41TDD719 pKa = 3.48QALITANQGPAIQIAKK735 pKa = 8.37TVAPDD740 pKa = 3.21TFGAVGTPITYY751 pKa = 10.15SYY753 pKa = 11.32QVTNTGNVTLNDD765 pKa = 3.66VQVTDD770 pKa = 3.84PHH772 pKa = 7.18DD773 pKa = 4.1GLSTISCSPSQGSSLDD789 pKa = 3.42PGSAMSCTASYY800 pKa = 11.11SATQADD806 pKa = 4.77LNIGSITDD814 pKa = 3.4TATVVGSPPTGPNVTDD830 pKa = 3.23SDD832 pKa = 4.04QALVTATTAPAIDD845 pKa = 3.64IQKK848 pKa = 10.04SAEE851 pKa = 3.94PTEE854 pKa = 4.63FSAAGTPIAYY864 pKa = 8.41TFVVTNTGNVTLDD877 pKa = 3.53NVDD880 pKa = 3.24VTDD883 pKa = 4.87PLDD886 pKa = 3.79GLTPIACDD894 pKa = 3.28PAQGVSLDD902 pKa = 4.45PGDD905 pKa = 5.11QMTCTAGYY913 pKa = 6.54TTTQADD919 pKa = 4.25LNRR922 pKa = 11.84GDD924 pKa = 3.82IANTATVVGVPPAGPNVSDD943 pKa = 5.07SDD945 pKa = 3.84QQLITANQGPEE956 pKa = 3.59IQITKK961 pKa = 9.95IADD964 pKa = 3.36PTEE967 pKa = 4.51FGSAGTPITYY977 pKa = 8.61TYY979 pKa = 11.53RR980 pKa = 11.84MINTGNVTVDD990 pKa = 3.69DD991 pKa = 4.01VRR993 pKa = 11.84ITDD996 pKa = 3.55PHH998 pKa = 7.46SGLSAISCAPAQGASLDD1015 pKa = 4.46PGDD1018 pKa = 5.41QMDD1021 pKa = 4.05CTAVYY1026 pKa = 7.57TTTQTDD1032 pKa = 3.48VNRR1035 pKa = 11.84GEE1037 pKa = 4.28IVNTATVTGTSPAGDD1052 pKa = 3.49VTDD1055 pKa = 3.76TDD1057 pKa = 4.17TALVTADD1064 pKa = 3.46QGPNITVTKK1073 pKa = 10.21IADD1076 pKa = 3.49PTEE1079 pKa = 4.42FGSAGTVINYY1089 pKa = 9.53GYY1091 pKa = 11.16LVTNNGNVTLDD1102 pKa = 3.82DD1103 pKa = 3.82VRR1105 pKa = 11.84VTDD1108 pKa = 4.03PQVGLSAVSCAPAQGASLAPGDD1130 pKa = 4.25TMSCTARR1137 pKa = 11.84YY1138 pKa = 6.14TTTQADD1144 pKa = 4.17LNAGLITNIGTVTGTPPTGPAVSDD1168 pKa = 3.57SDD1170 pKa = 4.27DD1171 pKa = 3.71AAVTANQVPAITISKK1186 pKa = 9.63IADD1189 pKa = 3.54PAEE1192 pKa = 4.44FGAAGTPITYY1202 pKa = 8.79TYY1204 pKa = 11.02RR1205 pKa = 11.84VTNTGNVTLDD1215 pKa = 3.7DD1216 pKa = 3.87VRR1218 pKa = 11.84VTDD1221 pKa = 3.47PHH1223 pKa = 7.47VGLSAVSCAPAQGATLEE1240 pKa = 5.19PGDD1243 pKa = 5.31QMDD1246 pKa = 3.87CTAAYY1251 pKa = 6.89TTTQADD1257 pKa = 4.23LNSGSVTNTATVVGQPPTGPNVTDD1281 pKa = 3.39TDD1283 pKa = 3.68QALITANQGPNIQIIKK1299 pKa = 9.21IAEE1302 pKa = 4.18PEE1304 pKa = 4.36TFGSAGTPITYY1315 pKa = 10.03SYY1317 pKa = 11.32QVTNTGNVTLDD1328 pKa = 3.7DD1329 pKa = 3.87VRR1331 pKa = 11.84VTDD1334 pKa = 3.47PHH1336 pKa = 7.47VGLSAVSCAPAQGSTLDD1353 pKa = 4.66PGDD1356 pKa = 3.52QMDD1359 pKa = 4.7CIGRR1363 pKa = 11.84YY1364 pKa = 6.44TTTQSDD1370 pKa = 4.18LNAGSVTDD1378 pKa = 3.65TATVTGEE1385 pKa = 4.24PPTGNAVTDD1394 pKa = 3.6SDD1396 pKa = 4.24QALITANQGPAITIQKK1412 pKa = 6.97TANPTEE1418 pKa = 4.48FGAAGTPITYY1428 pKa = 8.87TYY1430 pKa = 11.11QVTNTGNVTLDD1441 pKa = 3.7DD1442 pKa = 3.87VRR1444 pKa = 11.84VTDD1447 pKa = 3.47PHH1449 pKa = 7.47VGLSAVSCAPAQGATLDD1466 pKa = 4.73PGDD1469 pKa = 4.7QMDD1472 pKa = 4.05CTAVYY1477 pKa = 7.57TTTQGDD1483 pKa = 4.24LNSGSVTNTATVVGSPPTGPNVTDD1507 pKa = 3.39TDD1509 pKa = 3.68QALITANQGPAIQITKK1525 pKa = 8.65TAAPDD1530 pKa = 3.28TFGAVGTPITFTYY1543 pKa = 10.61QVTNTGNVTLDD1554 pKa = 3.7DD1555 pKa = 3.87VRR1557 pKa = 11.84VTDD1560 pKa = 3.47PHH1562 pKa = 7.47VGLSAVSCAPAQGSTLDD1579 pKa = 4.66PGDD1582 pKa = 3.52QMDD1585 pKa = 4.7CIGRR1589 pKa = 11.84YY1590 pKa = 6.44TTTQSDD1596 pKa = 4.18LNAGSVTDD1604 pKa = 3.65TATVTGEE1611 pKa = 4.28PPTGPAVTDD1620 pKa = 3.35SDD1622 pKa = 3.96QALITANQGPAITIQKK1638 pKa = 6.97TANPTEE1644 pKa = 4.48FGAAGTPITYY1654 pKa = 8.87TYY1656 pKa = 11.11QVTNTGNVTLDD1667 pKa = 3.7DD1668 pKa = 3.87VRR1670 pKa = 11.84VTDD1673 pKa = 3.47PHH1675 pKa = 7.47VGLSAVSCAPAQGATLEE1692 pKa = 5.19PGDD1695 pKa = 4.98QMDD1698 pKa = 3.92CTAVYY1703 pKa = 7.57TTTQGDD1709 pKa = 4.24LNSGSVTNTATVTGEE1724 pKa = 4.19PPTGNAVTDD1733 pKa = 3.6SDD1735 pKa = 4.24QALITANQGPAITTQKK1751 pKa = 8.61TANPTEE1757 pKa = 4.48FGAAGTPITYY1767 pKa = 8.87TYY1769 pKa = 11.11QVTNTGNVTLDD1780 pKa = 3.43NVRR1783 pKa = 11.84VTDD1786 pKa = 3.7PHH1788 pKa = 7.22AGLSAVSCAPAQGATLEE1805 pKa = 5.19PGDD1808 pKa = 5.03QMDD1811 pKa = 3.59CTGRR1815 pKa = 11.84YY1816 pKa = 5.68TTTQADD1822 pKa = 4.28LNSGSVTNTATVVGAPPTGPLVTDD1846 pKa = 3.23SDD1848 pKa = 4.45SAVVVANQAANIDD1861 pKa = 3.71ITKK1864 pKa = 10.38SAFPTEE1870 pKa = 4.72FGAAGTPINYY1880 pKa = 8.01TYY1882 pKa = 11.06QVTNNGNVTLDD1893 pKa = 3.6DD1894 pKa = 3.97VQVVDD1899 pKa = 4.29PHH1901 pKa = 7.47AGLSTIACNPPQRR1914 pKa = 11.84SSLDD1918 pKa = 3.42PGDD1921 pKa = 4.07TMQCTAEE1928 pKa = 4.16YY1929 pKa = 7.2TTTQADD1935 pKa = 4.09LNRR1938 pKa = 11.84GGIVNTATVTGTPPAGTEE1956 pKa = 4.09VTDD1959 pKa = 3.49SDD1961 pKa = 4.31SALVIANQSANINIIKK1977 pKa = 8.46TASPTEE1983 pKa = 4.18FGAAGTPITYY1993 pKa = 8.95TYY1995 pKa = 11.28LVTNNGNVTLDD2006 pKa = 3.78DD2007 pKa = 3.97VLVTDD2012 pKa = 4.13PHH2014 pKa = 7.13DD2015 pKa = 4.01GLSAITCDD2023 pKa = 3.49PAQGARR2029 pKa = 11.84LDD2031 pKa = 3.93PGEE2034 pKa = 4.91EE2035 pKa = 4.13MTCTADD2041 pKa = 3.33YY2042 pKa = 7.54TTTQDD2047 pKa = 4.51DD2048 pKa = 4.08LDD2050 pKa = 5.17AGEE2053 pKa = 5.15IINTGTTSGLPVTTDD2068 pKa = 2.92STSPRR2073 pKa = 11.84LPAALAAEE2081 pKa = 4.33RR2082 pKa = 11.84VSDD2085 pKa = 3.79SDD2087 pKa = 3.93TAIVTADD2094 pKa = 3.56QTPSIAISKK2103 pKa = 10.05SADD2106 pKa = 3.44PTSVDD2111 pKa = 3.21AAGDD2115 pKa = 3.87NIIYY2119 pKa = 10.05SYY2121 pKa = 11.2RR2122 pKa = 11.84VTNNGNVTLHH2132 pKa = 6.71DD2133 pKa = 3.78VRR2135 pKa = 11.84VTDD2138 pKa = 3.55PHH2140 pKa = 7.1QGLSDD2145 pKa = 3.76ISCEE2149 pKa = 3.85PAQGSNLEE2157 pKa = 4.22PGEE2160 pKa = 3.98QQVCTASYY2168 pKa = 8.05RR2169 pKa = 11.84TKK2171 pKa = 10.48QADD2174 pKa = 3.29IEE2176 pKa = 4.33RR2177 pKa = 11.84GSIEE2181 pKa = 4.12NVGTVAALTPNDD2193 pKa = 3.93TTVTASDD2200 pKa = 3.31TAMVSVVEE2208 pKa = 4.83KK2209 pKa = 8.07PTPPPTPTPTPSPPSPTPTCPQPPPATPSPSQPPTPPEE2247 pKa = 4.29SPTPPSTSPTPPPPCQPVATPPTSTPAPTSGPANLSGTGINPLVEE2292 pKa = 4.67TILGIGAALLLAGAVLLVVHH2312 pKa = 6.35LRR2314 pKa = 11.84RR2315 pKa = 11.84RR2316 pKa = 11.84RR2317 pKa = 11.84II2318 pKa = 3.33

Molecular weight: 234.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I5VMQ4|A0A6I5VMQ4_9ACTN Zinc-binding dehydrogenase OS=Microlunatus sp. Gsoil 973 OX=2672569 GN=GJV80_16285 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84THH17 pKa = 6.24GFRR20 pKa = 11.84HH21 pKa = 6.29RR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.68GRR40 pKa = 11.84ARR42 pKa = 11.84LAGG45 pKa = 3.63

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4311

0

4311

1389878

33

2504

322.4

34.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.169 ± 0.051

0.734 ± 0.01

6.445 ± 0.039

5.109 ± 0.039

2.917 ± 0.023

9.069 ± 0.029

2.161 ± 0.017

4.685 ± 0.025

2.006 ± 0.03

10.049 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.865 ± 0.015

2.12 ± 0.021

5.783 ± 0.03

3.223 ± 0.019

7.985 ± 0.048

5.625 ± 0.029

6.013 ± 0.035

8.309 ± 0.037

1.569 ± 0.015

2.165 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski