Amino acid dipepetide frequency for Microlunatus sp. Gsoil 973

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.101AlaAla: 18.101 ± 0.187	0.843AlaCys: 0.843 ± 0.023	8.657AlaAsp: 8.657 ± 0.086	7.896AlaGlu: 7.896 ± 0.093	3.173AlaPhe: 3.173 ± 0.051	12.235AlaGly: 12.235 ± 0.112	2.104AlaHis: 2.104 ± 0.04	5.265AlaIle: 5.265 ± 0.068	2.582AlaLys: 2.582 ± 0.051	11.317AlaLeu: 11.317 ± 0.119	2.497AlaMet: 2.497 ± 0.041	2.214AlaAsn: 2.214 ± 0.044	5.303AlaPro: 5.303 ± 0.069	3.369AlaGln: 3.369 ± 0.044	8.806AlaArg: 8.806 ± 0.089	5.677AlaSer: 5.677 ± 0.066	6.762AlaThr: 6.762 ± 0.081	10.777AlaVal: 10.777 ± 0.111	1.666AlaTrp: 1.666 ± 0.037	2.45AlaTyr: 2.45 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.779CysAla: 0.779 ± 0.029	0.115CysCys: 0.115 ± 0.009	0.426CysAsp: 0.426 ± 0.02	0.335CysGlu: 0.335 ± 0.018	0.235CysPhe: 0.235 ± 0.013	0.821CysGly: 0.821 ± 0.026	0.2CysHis: 0.2 ± 0.013	0.239CysIle: 0.239 ± 0.012	0.099CysLys: 0.099 ± 0.009	0.676CysLeu: 0.676 ± 0.022	0.119CysMet: 0.119 ± 0.009	0.142CysAsn: 0.142 ± 0.01	0.488CysPro: 0.488 ± 0.018	0.186CysGln: 0.186 ± 0.012	0.686CysArg: 0.686 ± 0.021	0.478CysSer: 0.478 ± 0.021	0.417CysThr: 0.417 ± 0.019	0.559CysVal: 0.559 ± 0.02	0.154CysTrp: 0.154 ± 0.011	0.189CysTyr: 0.189 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	6.938AspAla: 6.938 ± 0.071	0.408AspCys: 0.408 ± 0.02	4.405AspAsp: 4.405 ± 0.062	3.55AspGlu: 3.55 ± 0.052	1.827AspPhe: 1.827 ± 0.037	6.006AspGly: 6.006 ± 0.075	2.059AspHis: 2.059 ± 0.046	2.202AspIle: 2.202 ± 0.04	1.141AspLys: 1.141 ± 0.035	7.278AspLeu: 7.278 ± 0.077	0.725AspMet: 0.725 ± 0.024	1.172AspAsn: 1.172 ± 0.033	4.965AspPro: 4.965 ± 0.066	2.769AspGln: 2.769 ± 0.052	5.896AspArg: 5.896 ± 0.081	2.857AspSer: 2.857 ± 0.046	2.809AspThr: 2.809 ± 0.044	4.901AspVal: 4.901 ± 0.056	1.119AspTrp: 1.119 ± 0.03	1.422AspTyr: 1.422 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	5.862GluAla: 5.862 ± 0.071	0.287GluCys: 0.287 ± 0.014	2.561GluAsp: 2.561 ± 0.048	2.676GluGlu: 2.676 ± 0.056	1.589GluPhe: 1.589 ± 0.039	3.064GluGly: 3.064 ± 0.055	1.36GluHis: 1.36 ± 0.034	2.61GluIle: 2.61 ± 0.05	1.1GluLys: 1.1 ± 0.034	6.596GluLeu: 6.596 ± 0.089	0.876GluMet: 0.876 ± 0.028	1.017GluAsn: 1.017 ± 0.028	3.187GluPro: 3.187 ± 0.058	2.307GluGln: 2.307 ± 0.05	4.512GluArg: 4.512 ± 0.071	2.777GluSer: 2.777 ± 0.046	2.633GluThr: 2.633 ± 0.048	4.306GluVal: 4.306 ± 0.061	0.714GluTrp: 0.714 ± 0.024	1.053GluTyr: 1.053 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	3.652PheAla: 3.652 ± 0.055	0.272PheCys: 0.272 ± 0.013	2.192PheAsp: 2.192 ± 0.042	1.44PheGlu: 1.44 ± 0.037	0.948PhePhe: 0.948 ± 0.029	3.414PheGly: 3.414 ± 0.056	0.624PheHis: 0.624 ± 0.022	1.019PheIle: 1.019 ± 0.028	0.473PheLys: 0.473 ± 0.02	2.673PheLeu: 2.673 ± 0.055	0.394PheMet: 0.394 ± 0.02	0.684PheAsn: 0.684 ± 0.023	1.367PhePro: 1.367 ± 0.028	0.73PheGln: 0.73 ± 0.022	1.946PheArg: 1.946 ± 0.037	1.674PheSer: 1.674 ± 0.033	2.038PheThr: 2.038 ± 0.037	2.466PheVal: 2.466 ± 0.046	0.473PheTrp: 0.473 ± 0.02	0.689PheTyr: 0.689 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	9.007GlyAla: 9.007 ± 0.097	0.846GlyCys: 0.846 ± 0.023	5.064GlyAsp: 5.064 ± 0.057	4.302GlyGlu: 4.302 ± 0.06	3.143GlyPhe: 3.143 ± 0.056	7.552GlyGly: 7.552 ± 0.097	2.071GlyHis: 2.071 ± 0.038	4.275GlyIle: 4.275 ± 0.064	2.156GlyLys: 2.156 ± 0.054	9.457GlyLeu: 9.457 ± 0.102	2.048GlyMet: 2.048 ± 0.037	2.004GlyAsn: 2.004 ± 0.047	4.707GlyPro: 4.707 ± 0.074	2.905GlyGln: 2.905 ± 0.048	7.95GlyArg: 7.95 ± 0.09	5.95GlySer: 5.95 ± 0.062	5.318GlyThr: 5.318 ± 0.073	7.507GlyVal: 7.507 ± 0.075	1.902GlyTrp: 1.902 ± 0.035	2.52GlyTyr: 2.52 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.161HisAla: 2.161 ± 0.041	0.202HisCys: 0.202 ± 0.01	1.499HisAsp: 1.499 ± 0.039	0.968HisGlu: 0.968 ± 0.028	0.592HisPhe: 0.592 ± 0.02	2.151HisGly: 2.151 ± 0.039	0.677HisHis: 0.677 ± 0.02	0.71HisIle: 0.71 ± 0.022	0.333HisLys: 0.333 ± 0.015	2.29HisLeu: 2.29 ± 0.043	0.291HisMet: 0.291 ± 0.014	0.461HisAsn: 0.461 ± 0.019	1.724HisPro: 1.724 ± 0.036	0.836HisGln: 0.836 ± 0.022	2.193HisArg: 2.193 ± 0.043	1.05HisSer: 1.05 ± 0.027	1.096HisThr: 1.096 ± 0.03	1.441HisVal: 1.441 ± 0.038	0.399HisTrp: 0.399 ± 0.016	0.534HisTyr: 0.534 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	6.278IleAla: 6.278 ± 0.067	0.417IleCys: 0.417 ± 0.018	3.56IleAsp: 3.56 ± 0.055	2.517IleGlu: 2.517 ± 0.041	1.416IlePhe: 1.416 ± 0.04	5.216IleGly: 5.216 ± 0.066	0.73IleHis: 0.73 ± 0.024	2.25IleIle: 2.25 ± 0.042	1.132IleLys: 1.132 ± 0.027	3.277IleLeu: 3.277 ± 0.05	0.887IleMet: 0.887 ± 0.025	1.379IleAsn: 1.379 ± 0.035	2.265IlePro: 2.265 ± 0.043	0.95IleGln: 0.95 ± 0.028	3.091IleArg: 3.091 ± 0.043	2.776IleSer: 2.776 ± 0.047	3.627IleThr: 3.627 ± 0.056	3.682IleVal: 3.682 ± 0.058	0.571IleTrp: 0.571 ± 0.021	0.832IleTyr: 0.832 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	2.382LysAla: 2.382 ± 0.056	0.087LysCys: 0.087 ± 0.008	1.085LysAsp: 1.085 ± 0.037	0.882LysGlu: 0.882 ± 0.029	0.492LysPhe: 0.492 ± 0.019	1.443LysGly: 1.443 ± 0.038	0.43LysHis: 0.43 ± 0.017	1.43LysIle: 1.43 ± 0.036	0.722LysLys: 0.722 ± 0.036	1.953LysLeu: 1.953 ± 0.046	0.374LysMet: 0.374 ± 0.017	0.507LysAsn: 0.507 ± 0.021	1.322LysPro: 1.322 ± 0.033	0.792LysGln: 0.792 ± 0.026	1.33LysArg: 1.33 ± 0.038	1.173LysSer: 1.173 ± 0.031	1.228LysThr: 1.228 ± 0.04	1.685LysVal: 1.685 ± 0.045	0.271LysTrp: 0.271 ± 0.013	0.469LysTyr: 0.469 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	13.669LeuAla: 13.669 ± 0.117	0.725LeuCys: 0.725 ± 0.023	6.802LeuAsp: 6.802 ± 0.085	4.549LeuGlu: 4.549 ± 0.066	2.553LeuPhe: 2.553 ± 0.055	9.037LeuGly: 9.037 ± 0.098	2.039LeuHis: 2.039 ± 0.038	5.313LeuIle: 5.313 ± 0.073	1.683LeuLys: 1.683 ± 0.039	10.226LeuLeu: 10.226 ± 0.125	1.699LeuMet: 1.699 ± 0.037	1.937LeuAsn: 1.937 ± 0.037	5.919LeuPro: 5.919 ± 0.065	2.972LeuGln: 2.972 ± 0.051	7.667LeuArg: 7.667 ± 0.086	5.763LeuSer: 5.763 ± 0.067	6.312LeuThr: 6.312 ± 0.076	8.651LeuVal: 8.651 ± 0.092	1.252LeuTrp: 1.252 ± 0.036	1.72LeuTyr: 1.72 ± 0.035	0.0LeuXaa: 0.0 ± 0.0
Met	2.254MetAla: 2.254 ± 0.042	0.123MetCys: 0.123 ± 0.008	0.828MetAsp: 0.828 ± 0.025	0.654MetGlu: 0.654 ± 0.019	0.501MetPhe: 0.501 ± 0.021	1.226MetGly: 1.226 ± 0.031	0.37MetHis: 0.37 ± 0.014	1.547MetIle: 1.547 ± 0.039	0.391MetLys: 0.391 ± 0.017	1.886MetLeu: 1.886 ± 0.042	0.389MetMet: 0.389 ± 0.017	0.427MetAsn: 0.427 ± 0.016	1.217MetPro: 1.217 ± 0.031	0.512MetGln: 0.512 ± 0.017	1.306MetArg: 1.306 ± 0.031	1.417MetSer: 1.417 ± 0.032	1.62MetThr: 1.62 ± 0.033	1.455MetVal: 1.455 ± 0.036	0.198MetTrp: 0.198 ± 0.012	0.328MetTyr: 0.328 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.4AsnAla: 2.4 ± 0.042	0.155AsnCys: 0.155 ± 0.013	1.179AsnAsp: 1.179 ± 0.033	0.858AsnGlu: 0.858 ± 0.025	0.668AsnPhe: 0.668 ± 0.026	2.19AsnGly: 2.19 ± 0.048	0.457AsnHis: 0.457 ± 0.02	0.805AsnIle: 0.805 ± 0.027	0.456AsnLys: 0.456 ± 0.02	2.141AsnLeu: 2.141 ± 0.046	0.33AsnMet: 0.33 ± 0.017	0.577AsnAsn: 0.577 ± 0.023	1.607AsnPro: 1.607 ± 0.033	0.753AsnGln: 0.753 ± 0.027	1.676AsnArg: 1.676 ± 0.037	1.166AsnSer: 1.166 ± 0.032	1.262AsnThr: 1.262 ± 0.04	1.596AsnVal: 1.596 ± 0.039	0.366AsnTrp: 0.366 ± 0.014	0.56AsnTyr: 0.56 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	7.067ProAla: 7.067 ± 0.083	0.292ProCys: 0.292 ± 0.014	4.813ProAsp: 4.813 ± 0.074	3.746ProGlu: 3.746 ± 0.059	1.502ProPhe: 1.502 ± 0.039	5.908ProGly: 5.908 ± 0.072	1.168ProHis: 1.168 ± 0.03	2.299ProIle: 2.299 ± 0.045	1.15ProLys: 1.15 ± 0.033	4.682ProLeu: 4.682 ± 0.062	1.093ProMet: 1.093 ± 0.028	1.262ProAsn: 1.262 ± 0.034	3.007ProPro: 3.007 ± 0.063	1.835ProGln: 1.835 ± 0.038	3.736ProArg: 3.736 ± 0.051	3.203ProSer: 3.203 ± 0.055	3.714ProThr: 3.714 ± 0.06	5.011ProVal: 5.011 ± 0.069	0.899ProTrp: 0.899 ± 0.022	1.441ProTyr: 1.441 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.956GlnAla: 3.956 ± 0.055	0.175GlnCys: 0.175 ± 0.011	1.42GlnAsp: 1.42 ± 0.034	1.24GlnGlu: 1.24 ± 0.03	0.862GlnPhe: 0.862 ± 0.024	2.092GlnGly: 2.092 ± 0.047	0.786GlnHis: 0.786 ± 0.025	1.583GlnIle: 1.583 ± 0.033	0.644GlnLys: 0.644 ± 0.023	4.027GlnLeu: 4.027 ± 0.056	0.589GlnMet: 0.589 ± 0.025	0.645GlnAsn: 0.645 ± 0.02	2.202GlnPro: 2.202 ± 0.04	1.676GlnGln: 1.676 ± 0.044	2.871GlnArg: 2.871 ± 0.048	1.594GlnSer: 1.594 ± 0.033	1.842GlnThr: 1.842 ± 0.044	2.881GlnVal: 2.881 ± 0.045	0.481GlnTrp: 0.481 ± 0.019	0.665GlnTyr: 0.665 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	8.327ArgAla: 8.327 ± 0.093	0.583ArgCys: 0.583 ± 0.021	4.315ArgAsp: 4.315 ± 0.064	3.69ArgGlu: 3.69 ± 0.057	2.488ArgPhe: 2.488 ± 0.044	5.319ArgGly: 5.319 ± 0.067	1.929ArgHis: 1.929 ± 0.034	3.965ArgIle: 3.965 ± 0.058	1.535ArgLys: 1.535 ± 0.042	8.909ArgLeu: 8.909 ± 0.092	1.948ArgMet: 1.948 ± 0.032	1.692ArgAsn: 1.692 ± 0.034	4.974ArgPro: 4.974 ± 0.069	2.828ArgGln: 2.828 ± 0.049	8.284ArgArg: 8.284 ± 0.093	5.096ArgSer: 5.096 ± 0.066	4.882ArgThr: 4.882 ± 0.06	5.789ArgVal: 5.789 ± 0.069	1.415ArgTrp: 1.415 ± 0.032	1.879ArgTyr: 1.879 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.825SerAla: 6.825 ± 0.078	0.44SerCys: 0.44 ± 0.02	3.531SerAsp: 3.531 ± 0.055	2.674SerGlu: 2.674 ± 0.051	1.759SerPhe: 1.759 ± 0.042	6.245SerGly: 6.245 ± 0.07	1.02SerHis: 1.02 ± 0.025	2.429SerIle: 2.429 ± 0.043	1.076SerLys: 1.076 ± 0.031	4.887SerLeu: 4.887 ± 0.05	1.307SerMet: 1.307 ± 0.027	1.186SerAsn: 1.186 ± 0.03	3.167SerPro: 3.167 ± 0.057	1.592SerGln: 1.592 ± 0.032	4.32SerArg: 4.32 ± 0.06	3.323SerSer: 3.323 ± 0.058	3.412SerThr: 3.412 ± 0.055	4.508SerVal: 4.508 ± 0.06	1.069SerTrp: 1.069 ± 0.029	1.482SerTyr: 1.482 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	7.672ThrAla: 7.672 ± 0.111	0.371ThrCys: 0.371 ± 0.017	4.135ThrAsp: 4.135 ± 0.074	3.204ThrGlu: 3.204 ± 0.052	1.779ThrPhe: 1.779 ± 0.038	6.213ThrGly: 6.213 ± 0.065	1.082ThrHis: 1.082 ± 0.029	2.742ThrIle: 2.742 ± 0.047	1.24ThrLys: 1.24 ± 0.035	5.244ThrLeu: 5.244 ± 0.065	1.067ThrMet: 1.067 ± 0.03	1.294ThrAsn: 1.294 ± 0.035	3.659ThrPro: 3.659 ± 0.057	1.515ThrGln: 1.515 ± 0.034	3.895ThrArg: 3.895 ± 0.053	3.434ThrSer: 3.434 ± 0.054	3.887ThrThr: 3.887 ± 0.074	5.571ThrVal: 5.571 ± 0.079	0.929ThrTrp: 0.929 ± 0.025	1.194ThrTyr: 1.194 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	10.169ValAla: 10.169 ± 0.093	0.655ValCys: 0.655 ± 0.022	5.675ValAsp: 5.675 ± 0.061	4.296ValGlu: 4.296 ± 0.063	2.32ValPhe: 2.32 ± 0.044	7.301ValGly: 7.301 ± 0.072	1.651ValHis: 1.651 ± 0.034	4.818ValIle: 4.818 ± 0.076	1.443ValLys: 1.443 ± 0.032	8.641ValLeu: 8.641 ± 0.086	1.442ValMet: 1.442 ± 0.032	1.65ValAsn: 1.65 ± 0.034	4.646ValPro: 4.646 ± 0.066	2.147ValGln: 2.147 ± 0.037	6.408ValArg: 6.408 ± 0.065	4.695ValSer: 4.695 ± 0.062	5.179ValThr: 5.179 ± 0.091	7.521ValVal: 7.521 ± 0.079	1.056ValTrp: 1.056 ± 0.027	1.377ValTyr: 1.377 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.558TrpAla: 1.558 ± 0.031	0.16TrpCys: 0.16 ± 0.011	0.853TrpAsp: 0.853 ± 0.027	0.603TrpGlu: 0.603 ± 0.024	0.54TrpPhe: 0.54 ± 0.021	1.067TrpGly: 1.067 ± 0.029	0.393TrpHis: 0.393 ± 0.018	0.706TrpIle: 0.706 ± 0.023	0.34TrpLys: 0.34 ± 0.015	1.879TrpLeu: 1.879 ± 0.044	0.346TrpMet: 0.346 ± 0.019	0.455TrpAsn: 0.455 ± 0.018	0.846TrpPro: 0.846 ± 0.022	0.726TrpGln: 0.726 ± 0.023	1.33TrpArg: 1.33 ± 0.033	1.044TrpSer: 1.044 ± 0.028	1.004TrpThr: 1.004 ± 0.026	1.113TrpVal: 1.113 ± 0.03	0.38TrpTrp: 0.38 ± 0.016	0.352TrpTyr: 0.352 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.636TyrAla: 2.636 ± 0.045	0.187TyrCys: 0.187 ± 0.012	1.451TyrAsp: 1.451 ± 0.031	1.005TyrGlu: 1.005 ± 0.03	0.778TyrPhe: 0.778 ± 0.027	2.187TyrGly: 2.187 ± 0.041	0.462TyrHis: 0.462 ± 0.017	0.646TyrIle: 0.646 ± 0.021	0.361TyrLys: 0.361 ± 0.017	2.447TyrLeu: 2.447 ± 0.051	0.227TyrMet: 0.227 ± 0.014	0.499TyrAsn: 0.499 ± 0.021	1.258TyrPro: 1.258 ± 0.033	0.832TyrGln: 0.832 ± 0.027	1.944TyrArg: 1.944 ± 0.04	1.105TyrSer: 1.105 ± 0.03	1.084TyrThr: 1.084 ± 0.038	1.668TyrVal: 1.668 ± 0.037	0.379TyrTrp: 0.379 ± 0.017	0.493TyrTyr: 0.493 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4311 proteins (1389878 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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