Bacillus sp. X1(2014)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2943 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077J8Y4|A0A077J8Y4_9BACI Carbon starvation protein A OS=Bacillus sp. X1(2014) OX=1565991 GN=HW35_13410 PE=3 SV=1
MM1 pKa = 7.17ATKK4 pKa = 10.28CKK6 pKa = 10.29HH7 pKa = 6.26EE8 pKa = 4.1YY9 pKa = 9.82FCYY12 pKa = 10.3GLYY15 pKa = 10.29RR16 pKa = 11.84EE17 pKa = 4.2MKK19 pKa = 8.93EE20 pKa = 3.95YY21 pKa = 10.62EE22 pKa = 4.43DD23 pKa = 3.87YY24 pKa = 11.42VVTIRR29 pKa = 11.84TRR31 pKa = 11.84SGDD34 pKa = 4.1LIQGVLDD41 pKa = 4.11DD42 pKa = 3.92CTKK45 pKa = 10.26YY46 pKa = 10.99GIVKK50 pKa = 9.92IYY52 pKa = 10.15QQDD55 pKa = 3.57TLSPFMEE62 pKa = 4.38EE63 pKa = 3.33QLTFIRR69 pKa = 11.84IDD71 pKa = 4.1DD72 pKa = 3.65IEE74 pKa = 4.59YY75 pKa = 10.69FSVTDD80 pKa = 4.48VDD82 pKa = 6.24DD83 pKa = 6.8DD84 pKa = 5.76DD85 pKa = 7.71DD86 pKa = 6.94DD87 pKa = 4.68DD88 pKa = 4.1

Molecular weight: 10.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077J4Z6|A0A077J4Z6_9BACI Stage IV sporulation protein FB OS=Bacillus sp. X1(2014) OX=1565991 GN=HW35_05060 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.87VHH16 pKa = 5.77GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.48VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2943

0

2943

846451

30

2334

287.6

32.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.791 ± 0.041

0.741 ± 0.014

4.742 ± 0.029

7.642 ± 0.048

4.572 ± 0.04

6.876 ± 0.046

2.107 ± 0.021

8.223 ± 0.047

7.551 ± 0.041

9.839 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.803 ± 0.021

4.414 ± 0.029

3.67 ± 0.027

3.666 ± 0.036

4.184 ± 0.034

5.641 ± 0.03

5.187 ± 0.028

6.88 ± 0.037

1.005 ± 0.017

3.466 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski