Amino acid dipepetide frequency for Bacillus sp. X1(2014)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.787AlaAla: 5.787 ± 0.127	0.601AlaCys: 0.601 ± 0.028	3.152AlaAsp: 3.152 ± 0.073	4.648AlaGlu: 4.648 ± 0.093	3.107AlaPhe: 3.107 ± 0.062	5.206AlaGly: 5.206 ± 0.094	1.191AlaHis: 1.191 ± 0.033	5.951AlaIle: 5.951 ± 0.096	5.149AlaLys: 5.149 ± 0.084	6.943AlaLeu: 6.943 ± 0.092	2.017AlaMet: 2.017 ± 0.054	2.783AlaAsn: 2.783 ± 0.048	1.925AlaPro: 1.925 ± 0.052	2.117AlaGln: 2.117 ± 0.053	2.714AlaArg: 2.714 ± 0.066	3.595AlaSer: 3.595 ± 0.078	3.12AlaThr: 3.12 ± 0.062	5.173AlaVal: 5.173 ± 0.088	0.539AlaTrp: 0.539 ± 0.027	2.194AlaTyr: 2.194 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.452CysAla: 0.452 ± 0.025	0.092CysCys: 0.092 ± 0.011	0.397CysAsp: 0.397 ± 0.022	0.509CysGlu: 0.509 ± 0.024	0.336CysPhe: 0.336 ± 0.02	0.69CysGly: 0.69 ± 0.029	0.216CysHis: 0.216 ± 0.015	0.564CysIle: 0.564 ± 0.028	0.385CysLys: 0.385 ± 0.021	0.677CysLeu: 0.677 ± 0.033	0.18CysMet: 0.18 ± 0.014	0.323CysAsn: 0.323 ± 0.018	0.392CysPro: 0.392 ± 0.021	0.26CysGln: 0.26 ± 0.016	0.281CysArg: 0.281 ± 0.018	0.496CysSer: 0.496 ± 0.025	0.41CysThr: 0.41 ± 0.024	0.399CysVal: 0.399 ± 0.023	0.079CysTrp: 0.079 ± 0.01	0.273CysTyr: 0.273 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	2.952AspAla: 2.952 ± 0.063	0.416AspCys: 0.416 ± 0.023	2.049AspAsp: 2.049 ± 0.057	4.085AspGlu: 4.085 ± 0.072	2.355AspPhe: 2.355 ± 0.049	2.972AspGly: 2.972 ± 0.056	1.148AspHis: 1.148 ± 0.041	3.925AspIle: 3.925 ± 0.07	2.922AspLys: 2.922 ± 0.064	4.891AspLeu: 4.891 ± 0.079	1.3AspMet: 1.3 ± 0.037	1.511AspAsn: 1.511 ± 0.04	2.057AspPro: 2.057 ± 0.05	2.064AspGln: 2.064 ± 0.054	2.342AspArg: 2.342 ± 0.054	2.304AspSer: 2.304 ± 0.05	2.123AspThr: 2.123 ± 0.05	3.459AspVal: 3.459 ± 0.076	0.572AspTrp: 0.572 ± 0.027	1.974AspTyr: 1.974 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	5.271GluAla: 5.271 ± 0.101	0.39GluCys: 0.39 ± 0.019	3.304GluAsp: 3.304 ± 0.072	7.136GluGlu: 7.136 ± 0.108	2.785GluPhe: 2.785 ± 0.064	4.287GluGly: 4.287 ± 0.079	1.523GluHis: 1.523 ± 0.041	6.289GluIle: 6.289 ± 0.086	7.517GluLys: 7.517 ± 0.119	7.503GluLeu: 7.503 ± 0.107	2.441GluMet: 2.441 ± 0.057	3.857GluAsn: 3.857 ± 0.068	1.926GluPro: 1.926 ± 0.058	3.117GluGln: 3.117 ± 0.063	3.594GluArg: 3.594 ± 0.078	3.271GluSer: 3.271 ± 0.068	3.76GluThr: 3.76 ± 0.062	5.157GluVal: 5.157 ± 0.081	0.89GluTrp: 0.89 ± 0.033	2.399GluTyr: 2.399 ± 0.053	0.0GluXaa: 0.0 ± 0.0
Phe	2.944PheAla: 2.944 ± 0.062	0.346PheCys: 0.346 ± 0.022	2.288PheAsp: 2.288 ± 0.056	2.944PheGlu: 2.944 ± 0.068	2.403PhePhe: 2.403 ± 0.065	3.328PheGly: 3.328 ± 0.063	1.142PheHis: 1.142 ± 0.042	4.094PheIle: 4.094 ± 0.089	2.6PheLys: 2.6 ± 0.06	4.742PheLeu: 4.742 ± 0.097	1.148PheMet: 1.148 ± 0.037	1.837PheAsn: 1.837 ± 0.047	1.693PhePro: 1.693 ± 0.046	1.751PheGln: 1.751 ± 0.05	1.552PheArg: 1.552 ± 0.047	3.198PheSer: 3.198 ± 0.065	2.454PheThr: 2.454 ± 0.059	3.054PheVal: 3.054 ± 0.063	0.489PheTrp: 0.489 ± 0.027	1.714PheTyr: 1.714 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	4.709GlyAla: 4.709 ± 0.089	0.658GlyCys: 0.658 ± 0.029	2.989GlyAsp: 2.989 ± 0.062	4.394GlyGlu: 4.394 ± 0.074	3.384GlyPhe: 3.384 ± 0.073	4.8GlyGly: 4.8 ± 0.096	1.431GlyHis: 1.431 ± 0.041	6.232GlyIle: 6.232 ± 0.096	5.397GlyLys: 5.397 ± 0.084	6.703GlyLeu: 6.703 ± 0.087	2.144GlyMet: 2.144 ± 0.056	2.727GlyAsn: 2.727 ± 0.063	1.94GlyPro: 1.94 ± 0.104	2.098GlyGln: 2.098 ± 0.049	2.802GlyArg: 2.802 ± 0.061	3.771GlySer: 3.771 ± 0.069	3.914GlyThr: 3.914 ± 0.076	5.123GlyVal: 5.123 ± 0.084	0.799GlyTrp: 0.799 ± 0.037	2.75GlyTyr: 2.75 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	1.193HisAla: 1.193 ± 0.038	0.209HisCys: 0.209 ± 0.015	1.031HisAsp: 1.031 ± 0.035	1.374HisGlu: 1.374 ± 0.047	1.055HisPhe: 1.055 ± 0.045	1.451HisGly: 1.451 ± 0.043	0.671HisHis: 0.671 ± 0.032	1.549HisIle: 1.549 ± 0.043	1.177HisLys: 1.177 ± 0.038	2.196HisLeu: 2.196 ± 0.048	0.483HisMet: 0.483 ± 0.023	0.771HisAsn: 0.771 ± 0.028	1.206HisPro: 1.206 ± 0.037	0.899HisGln: 0.899 ± 0.035	0.919HisArg: 0.919 ± 0.034	1.307HisSer: 1.307 ± 0.04	1.035HisThr: 1.035 ± 0.034	1.431HisVal: 1.431 ± 0.047	0.219HisTrp: 0.219 ± 0.017	0.892HisTyr: 0.892 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	6.126IleAla: 6.126 ± 0.094	0.659IleCys: 0.659 ± 0.031	4.212IleAsp: 4.212 ± 0.074	6.067IleGlu: 6.067 ± 0.101	3.614IlePhe: 3.614 ± 0.077	6.287IleGly: 6.287 ± 0.098	1.908IleHis: 1.908 ± 0.051	6.637IleIle: 6.637 ± 0.11	5.228IleLys: 5.228 ± 0.072	7.757IleLeu: 7.757 ± 0.126	1.919IleMet: 1.919 ± 0.05	3.472IleAsn: 3.472 ± 0.073	3.551IlePro: 3.551 ± 0.07	3.134IleGln: 3.134 ± 0.07	3.404IleArg: 3.404 ± 0.06	5.095IleSer: 5.095 ± 0.081	4.349IleThr: 4.349 ± 0.068	5.54IleVal: 5.54 ± 0.084	0.662IleTrp: 0.662 ± 0.031	2.606IleTyr: 2.606 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	5.021LysAla: 5.021 ± 0.092	0.384LysCys: 0.384 ± 0.024	3.851LysAsp: 3.851 ± 0.067	7.641LysGlu: 7.641 ± 0.101	2.227LysPhe: 2.227 ± 0.05	4.852LysGly: 4.852 ± 0.08	1.346LysHis: 1.346 ± 0.041	5.581LysIle: 5.581 ± 0.1	6.804LysLys: 6.804 ± 0.109	6.506LysLeu: 6.506 ± 0.086	2.497LysMet: 2.497 ± 0.052	3.933LysAsn: 3.933 ± 0.079	2.427LysPro: 2.427 ± 0.049	3.169LysGln: 3.169 ± 0.074	3.569LysArg: 3.569 ± 0.067	3.552LysSer: 3.552 ± 0.061	3.816LysThr: 3.816 ± 0.065	5.058LysVal: 5.058 ± 0.076	0.88LysTrp: 0.88 ± 0.03	2.397LysTyr: 2.397 ± 0.049	0.0LysXaa: 0.0 ± 0.0
Leu	7.163LeuAla: 7.163 ± 0.099	0.636LeuCys: 0.636 ± 0.027	4.657LeuAsp: 4.657 ± 0.075	7.032LeuGlu: 7.032 ± 0.095	4.798LeuPhe: 4.798 ± 0.092	6.558LeuGly: 6.558 ± 0.098	1.903LeuHis: 1.903 ± 0.051	7.543LeuIle: 7.543 ± 0.108	7.735LeuLys: 7.735 ± 0.102	10.138LeuLeu: 10.138 ± 0.137	2.611LeuMet: 2.611 ± 0.057	4.322LeuAsn: 4.322 ± 0.077	3.87LeuPro: 3.87 ± 0.063	3.439LeuGln: 3.439 ± 0.072	3.697LeuArg: 3.697 ± 0.083	6.567LeuSer: 6.567 ± 0.095	5.635LeuThr: 5.635 ± 0.075	6.235LeuVal: 6.235 ± 0.094	0.794LeuTrp: 0.794 ± 0.036	3.059LeuTyr: 3.059 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	2.171MetAla: 2.171 ± 0.053	0.169MetCys: 0.169 ± 0.015	1.481MetAsp: 1.481 ± 0.041	2.293MetGlu: 2.293 ± 0.051	1.103MetPhe: 1.103 ± 0.039	1.826MetGly: 1.826 ± 0.05	0.417MetHis: 0.417 ± 0.021	2.336MetIle: 2.336 ± 0.054	2.612MetLys: 2.612 ± 0.056	2.422MetLeu: 2.422 ± 0.052	0.857MetMet: 0.857 ± 0.034	1.526MetAsn: 1.526 ± 0.042	1.009MetPro: 1.009 ± 0.039	0.857MetGln: 0.857 ± 0.035	1.025MetArg: 1.025 ± 0.038	1.595MetSer: 1.595 ± 0.048	1.616MetThr: 1.616 ± 0.041	1.858MetVal: 1.858 ± 0.043	0.167MetTrp: 0.167 ± 0.013	0.692MetTyr: 0.692 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.665AsnAla: 2.665 ± 0.057	0.326AsnCys: 0.326 ± 0.019	2.037AsnAsp: 2.037 ± 0.053	3.642AsnGlu: 3.642 ± 0.058	1.672AsnPhe: 1.672 ± 0.039	3.393AsnGly: 3.393 ± 0.078	1.157AsnHis: 1.157 ± 0.037	3.541AsnIle: 3.541 ± 0.066	3.195AsnLys: 3.195 ± 0.063	3.94AsnLeu: 3.94 ± 0.066	1.186AsnMet: 1.186 ± 0.038	1.953AsnAsn: 1.953 ± 0.053	2.213AsnPro: 2.213 ± 0.052	2.032AsnGln: 2.032 ± 0.051	2.124AsnArg: 2.124 ± 0.055	2.193AsnSer: 2.193 ± 0.049	2.006AsnThr: 2.006 ± 0.05	2.832AsnVal: 2.832 ± 0.062	0.559AsnTrp: 0.559 ± 0.028	1.472AsnTyr: 1.472 ± 0.045	0.0AsnXaa: 0.0 ± 0.0
Pro	2.114ProAla: 2.114 ± 0.058	0.226ProCys: 0.226 ± 0.016	1.967ProAsp: 1.967 ± 0.045	3.094ProGlu: 3.094 ± 0.06	2.017ProPhe: 2.017 ± 0.051	2.386ProGly: 2.386 ± 0.059	0.794ProHis: 0.794 ± 0.036	3.1ProIle: 3.1 ± 0.064	2.443ProLys: 2.443 ± 0.061	3.517ProLeu: 3.517 ± 0.069	0.855ProMet: 0.855 ± 0.034	1.862ProAsn: 1.862 ± 0.046	1.047ProPro: 1.047 ± 0.035	1.125ProGln: 1.125 ± 0.037	1.139ProArg: 1.139 ± 0.036	2.262ProSer: 2.262 ± 0.053	2.072ProThr: 2.072 ± 0.106	2.851ProVal: 2.851 ± 0.061	0.396ProTrp: 0.396 ± 0.023	1.432ProTyr: 1.432 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.57GlnAla: 2.57 ± 0.056	0.221GlnCys: 0.221 ± 0.016	1.415GlnAsp: 1.415 ± 0.042	2.988GlnGlu: 2.988 ± 0.06	1.691GlnPhe: 1.691 ± 0.046	2.125GlnGly: 2.125 ± 0.05	0.719GlnHis: 0.719 ± 0.029	2.762GlnIle: 2.762 ± 0.067	3.0GlnLys: 3.0 ± 0.066	4.114GlnLeu: 4.114 ± 0.08	1.151GlnMet: 1.151 ± 0.038	1.684GlnAsn: 1.684 ± 0.047	1.191GlnPro: 1.191 ± 0.041	1.64GlnGln: 1.64 ± 0.048	1.498GlnArg: 1.498 ± 0.044	1.945GlnSer: 1.945 ± 0.049	1.867GlnThr: 1.867 ± 0.053	2.365GlnVal: 2.365 ± 0.056	0.388GlnTrp: 0.388 ± 0.023	1.329GlnTyr: 1.329 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	2.475ArgAla: 2.475 ± 0.065	0.262ArgCys: 0.262 ± 0.018	1.976ArgAsp: 1.976 ± 0.051	3.438ArgGlu: 3.438 ± 0.058	1.988ArgPhe: 1.988 ± 0.045	2.441ArgGly: 2.441 ± 0.063	0.86ArgHis: 0.86 ± 0.035	3.329ArgIle: 3.329 ± 0.06	3.673ArgLys: 3.673 ± 0.068	4.09ArgLeu: 4.09 ± 0.081	1.341ArgMet: 1.341 ± 0.039	1.94ArgAsn: 1.94 ± 0.052	1.425ArgPro: 1.425 ± 0.045	1.526ArgGln: 1.526 ± 0.049	2.123ArgArg: 2.123 ± 0.059	2.128ArgSer: 2.128 ± 0.053	2.091ArgThr: 2.091 ± 0.046	2.671ArgVal: 2.671 ± 0.059	0.423ArgTrp: 0.423 ± 0.021	1.642ArgTyr: 1.642 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	3.402SerAla: 3.402 ± 0.063	0.405SerCys: 0.405 ± 0.022	2.527SerAsp: 2.527 ± 0.056	3.692SerGlu: 3.692 ± 0.059	3.169SerPhe: 3.169 ± 0.06	4.245SerGly: 4.245 ± 0.086	1.201SerHis: 1.201 ± 0.041	4.931SerIle: 4.931 ± 0.089	3.975SerLys: 3.975 ± 0.079	5.746SerLeu: 5.746 ± 0.081	1.663SerMet: 1.663 ± 0.045	2.398SerAsn: 2.398 ± 0.06	2.089SerPro: 2.089 ± 0.046	1.9SerGln: 1.9 ± 0.053	2.364SerArg: 2.364 ± 0.056	3.394SerSer: 3.394 ± 0.06	2.827SerThr: 2.827 ± 0.062	3.825SerVal: 3.825 ± 0.065	0.569SerTrp: 0.569 ± 0.024	2.086SerTyr: 2.086 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	3.746ThrAla: 3.746 ± 0.077	0.393ThrCys: 0.393 ± 0.024	2.438ThrAsp: 2.438 ± 0.049	3.386ThrGlu: 3.386 ± 0.065	2.52ThrPhe: 2.52 ± 0.06	4.258ThrGly: 4.258 ± 0.113	0.979ThrHis: 0.979 ± 0.032	4.648ThrIle: 4.648 ± 0.077	3.594ThrLys: 3.594 ± 0.071	4.951ThrLeu: 4.951 ± 0.073	1.32ThrMet: 1.32 ± 0.043	2.338ThrAsn: 2.338 ± 0.065	2.282ThrPro: 2.282 ± 0.052	1.245ThrGln: 1.245 ± 0.038	1.854ThrArg: 1.854 ± 0.046	2.907ThrSer: 2.907 ± 0.06	2.726ThrThr: 2.726 ± 0.059	3.994ThrVal: 3.994 ± 0.071	0.481ThrTrp: 0.481 ± 0.025	1.806ThrTyr: 1.806 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	4.687ValAla: 4.687 ± 0.092	0.616ValCys: 0.616 ± 0.029	3.363ValAsp: 3.363 ± 0.069	4.847ValGlu: 4.847 ± 0.082	3.087ValPhe: 3.087 ± 0.065	4.544ValGly: 4.544 ± 0.072	1.341ValHis: 1.341 ± 0.046	5.912ValIle: 5.912 ± 0.092	5.08ValLys: 5.08 ± 0.081	6.79ValLeu: 6.79 ± 0.102	1.792ValMet: 1.792 ± 0.044	3.049ValAsn: 3.049 ± 0.067	2.766ValPro: 2.766 ± 0.056	2.273ValGln: 2.273 ± 0.055	2.801ValArg: 2.801 ± 0.065	4.29ValSer: 4.29 ± 0.082	3.903ValThr: 3.903 ± 0.065	4.917ValVal: 4.917 ± 0.089	0.59ValTrp: 0.59 ± 0.028	2.156ValTyr: 2.156 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.58TrpAla: 0.58 ± 0.029	0.099TrpCys: 0.099 ± 0.01	0.493TrpAsp: 0.493 ± 0.021	0.625TrpGlu: 0.625 ± 0.024	0.517TrpPhe: 0.517 ± 0.029	0.652TrpGly: 0.652 ± 0.028	0.202TrpHis: 0.202 ± 0.016	0.832TrpIle: 0.832 ± 0.031	0.866TrpLys: 0.866 ± 0.041	1.112TrpLeu: 1.112 ± 0.037	0.344TrpMet: 0.344 ± 0.021	0.506TrpAsn: 0.506 ± 0.025	0.271TrpPro: 0.271 ± 0.02	0.364TrpGln: 0.364 ± 0.024	0.405TrpArg: 0.405 ± 0.019	0.553TrpSer: 0.553 ± 0.026	0.436TrpThr: 0.436 ± 0.023	0.729TrpVal: 0.729 ± 0.031	0.121TrpTrp: 0.121 ± 0.012	0.343TrpTyr: 0.343 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.882TyrAla: 1.882 ± 0.051	0.304TyrCys: 0.304 ± 0.017	1.79TyrAsp: 1.79 ± 0.049	2.583TyrGlu: 2.583 ± 0.056	1.897TyrPhe: 1.897 ± 0.047	2.461TyrGly: 2.461 ± 0.052	0.918TyrHis: 0.918 ± 0.032	2.473TyrIle: 2.473 ± 0.061	2.161TyrLys: 2.161 ± 0.052	3.653TyrLeu: 3.653 ± 0.064	0.784TyrMet: 0.784 ± 0.029	1.344TyrAsn: 1.344 ± 0.038	1.42TyrPro: 1.42 ± 0.041	1.652TyrGln: 1.652 ± 0.041	1.636TyrArg: 1.636 ± 0.049	1.987TyrSer: 1.987 ± 0.05	1.707TyrThr: 1.707 ± 0.049	2.131TyrVal: 2.131 ± 0.051	0.435TyrTrp: 0.435 ± 0.025	1.444TyrTyr: 1.444 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2943 proteins (846451 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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