Wenxinia marina DSM 24838
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4030 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D0Q8B7|A0A0D0Q8B7_9RHOB Mg chelatase-related protein OS=Wenxinia marina DSM 24838 OX=1123501 GN=Wenmar_01022 PE=3 SV=1
MM1 pKa = 7.2 TADD4 pKa = 3.67 NNTDD8 pKa = 3.35 GEE10 pKa = 4.32 IHH12 pKa = 6.51 MKK14 pKa = 10.46 RR15 pKa = 11.84 LLLSSAALLLPAGTALADD33 pKa = 3.88 FSMTILHH40 pKa = 6.41 TNDD43 pKa = 2.48 FHH45 pKa = 8.99 DD46 pKa = 3.87 RR47 pKa = 11.84 FEE49 pKa = 5.48 PISAFDD55 pKa = 3.7 STCDD59 pKa = 3.71 AEE61 pKa = 4.37 TNAAGEE67 pKa = 4.58 CFGGTARR74 pKa = 11.84 LVTAIEE80 pKa = 3.75 QARR83 pKa = 11.84 AEE85 pKa = 4.18 NEE87 pKa = 3.7 NTILVDD93 pKa = 5.05 GGDD96 pKa = 3.59 QFQGTLFYY104 pKa = 10.03 TYY106 pKa = 10.75 YY107 pKa = 9.55 KK108 pKa = 11.14 GSMTAEE114 pKa = 4.31 FMNQLGYY121 pKa = 10.99 DD122 pKa = 3.29 AMTVGNHH129 pKa = 5.62 EE130 pKa = 4.45 FDD132 pKa = 5.23 DD133 pKa = 5.09 GPEE136 pKa = 3.95 VLRR139 pKa = 11.84 EE140 pKa = 3.92 FVDD143 pKa = 3.92 TVEE146 pKa = 5.11 FPILMSNADD155 pKa = 3.52 VSGEE159 pKa = 4.03 PLLADD164 pKa = 5.56 AIMKK168 pKa = 7.98 STTIEE173 pKa = 4.04 VGGEE177 pKa = 4.12 TIGLIGLTPQDD188 pKa = 3.73 TDD190 pKa = 3.46 EE191 pKa = 4.75 LASPGPNVIFTDD203 pKa = 3.99 PSDD206 pKa = 3.63 AVQAEE211 pKa = 4.12 VDD213 pKa = 3.57 ALTEE217 pKa = 4.16 AGVNKK222 pKa = 9.98 IVVLSHH228 pKa = 6.42 SGYY231 pKa = 11.36 GLDD234 pKa = 3.37 QTVAEE239 pKa = 4.49 NTTGVDD245 pKa = 3.88 VIVGGHH251 pKa = 5.65 TNTLLGDD258 pKa = 3.52 MEE260 pKa = 4.8 GAQGAYY266 pKa = 7.34 PTMVGDD272 pKa = 3.75 TAIVQAYY279 pKa = 10.11 AYY281 pKa = 9.78 GKK283 pKa = 9.11 YY284 pKa = 10.4 LGMLDD289 pKa = 3.66 VTFDD293 pKa = 3.72 DD294 pKa = 4.99 DD295 pKa = 4.71 GNIIEE300 pKa = 5.6 ASGQPLLIDD309 pKa = 3.91 ASVEE313 pKa = 4.1 EE314 pKa = 4.26 NQGVLDD320 pKa = 4.6 QIAEE324 pKa = 4.2 YY325 pKa = 10.23 AAPLDD330 pKa = 4.23 EE331 pKa = 4.61 IRR333 pKa = 11.84 NEE335 pKa = 4.23 VVAEE339 pKa = 3.89 TSEE342 pKa = 4.67 AIEE345 pKa = 4.34 GNRR348 pKa = 11.84 DD349 pKa = 2.33 ICRR352 pKa = 11.84 AMEE355 pKa = 4.17 CSMGNLVADD364 pKa = 4.1 AMLDD368 pKa = 3.4 RR369 pKa = 11.84 VADD372 pKa = 3.5 QGIDD376 pKa = 3.43 VAIANSGGLRR386 pKa = 11.84 ASIDD390 pKa = 3.41 AGEE393 pKa = 4.19 VTMGEE398 pKa = 4.52 VLTVLPFQNTLSTFQITGAQLMEE421 pKa = 4.32 ALEE424 pKa = 4.5 NGVSQVEE431 pKa = 4.34 EE432 pKa = 4.13 GAGRR436 pKa = 11.84 FPQIAGMTFTADD448 pKa = 3.74 LSQEE452 pKa = 3.9 PGSRR456 pKa = 11.84 ITEE459 pKa = 4.02 VTVGGEE465 pKa = 4.02 PLDD468 pKa = 4.17 PEE470 pKa = 4.19 ATYY473 pKa = 10.78 GAVSNNYY480 pKa = 8.3 VRR482 pKa = 11.84 NGGDD486 pKa = 3.63 GYY488 pKa = 11.6 SMFVNAEE495 pKa = 3.72 NAYY498 pKa = 10.81 DD499 pKa = 4.41 FGPDD503 pKa = 3.4 LADD506 pKa = 3.24 VTAEE510 pKa = 3.85 YY511 pKa = 10.49 LAANGPYY518 pKa = 9.49 TPYY521 pKa = 9.58 TDD523 pKa = 3.45 GRR525 pKa = 11.84 ITIEE529 pKa = 3.86 NAPAAEE535 pKa = 4.02
Molecular weight: 56.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.681
IPC_protein 3.706
Toseland 3.49
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.592
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 3.999
Thurlkill 3.528
EMBOSS 3.605
Sillero 3.821
Patrickios 1.354
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.758
Protein with the highest isoelectric point:
>tr|A0A0D0Q484|A0A0D0Q484_9RHOB GTPase Der OS=Wenxinia marina DSM 24838 OX=1123501 GN=der PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNRR11 pKa = 11.84 VRR13 pKa = 11.84 KK14 pKa = 9.2 ARR16 pKa = 11.84 HH17 pKa = 4.71 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.43 GGRR29 pKa = 11.84 KK30 pKa = 8.99 VLNARR35 pKa = 11.84 RR36 pKa = 11.84 ARR38 pKa = 11.84 GRR40 pKa = 11.84 KK41 pKa = 9.03 RR42 pKa = 11.84 LSAA45 pKa = 4.03
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4030
0
4030
1236898
24
7873
306.9
33.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.692 ± 0.06
0.808 ± 0.012
6.179 ± 0.044
6.101 ± 0.037
3.422 ± 0.027
9.382 ± 0.044
1.918 ± 0.02
4.508 ± 0.029
2.064 ± 0.033
10.178 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.494 ± 0.02
2.047 ± 0.022
5.604 ± 0.038
2.65 ± 0.02
7.781 ± 0.049
4.575 ± 0.032
5.413 ± 0.032
7.591 ± 0.036
1.534 ± 0.019
2.056 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here