Brevundimonas sp. M20

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Brevundimonas; unclassified Brevundimonas

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3160 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514C4J0|A0A514C4J0_9CAUL Phosphonates import ATP-binding protein PhnC OS=Brevundimonas sp. M20 OX=2591463 GN=phnC PE=3 SV=1
MM1 pKa = 7.76SDD3 pKa = 2.9RR4 pKa = 11.84TAALPAGTALPPTLPLMADD23 pKa = 3.48DD24 pKa = 5.24RR25 pKa = 11.84FAPGITEE32 pKa = 4.07TGDD35 pKa = 3.22AGDD38 pKa = 3.47NFLYY42 pKa = 8.91GTHH45 pKa = 6.97NDD47 pKa = 3.43DD48 pKa = 4.44TLNGMAGNDD57 pKa = 3.63VLNGEE62 pKa = 5.29DD63 pKa = 4.38GYY65 pKa = 11.75DD66 pKa = 3.4WLIGGAGNDD75 pKa = 3.69VFNGGDD81 pKa = 3.78GIDD84 pKa = 3.54TGDD87 pKa = 3.59YY88 pKa = 9.89SAAASGVTARR98 pKa = 11.84LDD100 pKa = 3.02IGRR103 pKa = 11.84ATNDD107 pKa = 2.78GDD109 pKa = 4.02GGVDD113 pKa = 2.87ILNSIEE119 pKa = 4.11RR120 pKa = 11.84LYY122 pKa = 11.43GSAFNDD128 pKa = 3.36VLIGDD133 pKa = 4.22GQDD136 pKa = 3.11NLLVGGAGYY145 pKa = 10.74DD146 pKa = 3.63VLIGGAGNDD155 pKa = 3.61RR156 pKa = 11.84IYY158 pKa = 11.27GGVGAANEE166 pKa = 4.38LYY168 pKa = 10.73GGAGDD173 pKa = 3.73DD174 pKa = 4.48TFYY177 pKa = 11.61LDD179 pKa = 4.88ANDD182 pKa = 4.11TVVEE186 pKa = 4.06LAGGGIDD193 pKa = 3.51TVRR196 pKa = 11.84TSLTLVNLAANVEE209 pKa = 4.24NLWYY213 pKa = 10.1DD214 pKa = 4.01GPQDD218 pKa = 3.49FTGNGNAGDD227 pKa = 3.93NVITGGSGNDD237 pKa = 3.38VLRR240 pKa = 11.84GGVGNDD246 pKa = 3.35TLNGRR251 pKa = 11.84GGMDD255 pKa = 3.02TADD258 pKa = 3.8YY259 pKa = 8.91STAAAGIHH267 pKa = 6.23ARR269 pKa = 11.84LDD271 pKa = 3.34YY272 pKa = 8.15MTAFNDD278 pKa = 3.44GDD280 pKa = 4.21GGVDD284 pKa = 3.17TYY286 pKa = 11.97VDD288 pKa = 3.23IEE290 pKa = 4.94AIVGSAFDD298 pKa = 4.22DD299 pKa = 4.22LLVGGALNDD308 pKa = 3.96HH309 pKa = 6.81LSGGLGRR316 pKa = 11.84DD317 pKa = 3.4TLLGGTGNDD326 pKa = 3.53VLSGGQGVANTLQGGVGDD344 pKa = 4.75DD345 pKa = 4.91RR346 pKa = 11.84YY347 pKa = 11.03ILDD350 pKa = 4.06ANDD353 pKa = 4.33TIVEE357 pKa = 4.07LAGEE361 pKa = 4.45GFDD364 pKa = 3.47TVEE367 pKa = 3.97VHH369 pKa = 6.16IGAYY373 pKa = 10.07VMAANLEE380 pKa = 3.94NMEE383 pKa = 4.36YY384 pKa = 10.55VGQTTFNGTGNAGNNNIYY402 pKa = 10.77GGDD405 pKa = 3.77LNDD408 pKa = 3.46VLRR411 pKa = 11.84GGGGDD416 pKa = 3.31DD417 pKa = 3.24HH418 pKa = 8.34LYY420 pKa = 10.79GGRR423 pKa = 11.84GDD425 pKa = 3.69DD426 pKa = 3.59TVVLRR431 pKa = 11.84GAAGDD436 pKa = 3.75YY437 pKa = 9.53TITGDD442 pKa = 3.45GVGYY446 pKa = 10.19RR447 pKa = 11.84IVDD450 pKa = 3.93AVPGRR455 pKa = 11.84DD456 pKa = 2.8GSTYY460 pKa = 8.33VTGIEE465 pKa = 4.32TVLFEE470 pKa = 5.51ADD472 pKa = 3.02GTTRR476 pKa = 11.84TLSYY480 pKa = 9.6TPPPAPSEE488 pKa = 4.8LIDD491 pKa = 3.62KK492 pKa = 10.7ADD494 pKa = 3.7YY495 pKa = 11.4GDD497 pKa = 3.93AQVLPGVIDD506 pKa = 4.56DD507 pKa = 4.87AFLDD511 pKa = 3.7LGGVDD516 pKa = 5.93FGPQTLPPVFDD527 pKa = 5.11DD528 pKa = 6.15FLVLDD533 pKa = 5.55DD534 pKa = 5.0GLSDD538 pKa = 4.13PVWIAAAHH546 pKa = 5.53NAAPEE551 pKa = 4.01IQHH554 pKa = 6.62ILDD557 pKa = 4.01IFHH560 pKa = 7.26GDD562 pKa = 3.57DD563 pKa = 4.13AGHH566 pKa = 6.91HH567 pKa = 6.18GPFNAVDD574 pKa = 3.75PWAA577 pKa = 4.71

Molecular weight: 58.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514BYB4|A0A514BYB4_9CAUL Uncharacterized protein OS=Brevundimonas sp. M20 OX=2591463 GN=FKQ52_02420 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.05NGQKK29 pKa = 9.47IVQRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3160

0

3160

993302

30

2525

314.3

33.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.394 ± 0.06

0.696 ± 0.012

6.055 ± 0.038

5.712 ± 0.042

3.473 ± 0.023

9.146 ± 0.052

1.748 ± 0.022

4.455 ± 0.029

2.711 ± 0.042

9.85 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.02

2.435 ± 0.029

5.503 ± 0.035

3.095 ± 0.023

7.533 ± 0.049

5.11 ± 0.031

5.584 ± 0.04

7.657 ± 0.034

1.502 ± 0.017

2.053 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski