Amino acid dipepetide frequency for Brevundimonas sp. M20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.768AlaAla: 19.768 ± 0.205
1.104AlaCys: 1.104 ± 0.035
8.012AlaAsp: 8.012 ± 0.093
8.114AlaGlu: 8.114 ± 0.102
4.822AlaPhe: 4.822 ± 0.075
11.766AlaGly: 11.766 ± 0.121
2.139AlaHis: 2.139 ± 0.052
5.308AlaIle: 5.308 ± 0.066
3.3AlaLys: 3.3 ± 0.073
14.213AlaLeu: 14.213 ± 0.155
3.483AlaMet: 3.483 ± 0.073
2.821AlaAsn: 2.821 ± 0.07
7.386AlaPro: 7.386 ± 0.118
4.098AlaGln: 4.098 ± 0.078
10.232AlaArg: 10.232 ± 0.139
6.545AlaSer: 6.545 ± 0.09
6.407AlaThr: 6.407 ± 0.082
10.124AlaVal: 10.124 ± 0.115
1.795AlaTrp: 1.795 ± 0.044
2.502AlaTyr: 2.502 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.893CysAla: 0.893 ± 0.033
0.071CysCys: 0.071 ± 0.009
0.45CysAsp: 0.45 ± 0.019
0.376CysGlu: 0.376 ± 0.022
0.21CysPhe: 0.21 ± 0.015
0.805CysGly: 0.805 ± 0.03
0.168CysHis: 0.168 ± 0.013
0.268CysIle: 0.268 ± 0.016
0.141CysLys: 0.141 ± 0.011
0.673CysLeu: 0.673 ± 0.031
0.122CysMet: 0.122 ± 0.011
0.172CysAsn: 0.172 ± 0.015
0.423CysPro: 0.423 ± 0.024
0.167CysGln: 0.167 ± 0.014
0.525CysArg: 0.525 ± 0.023
0.343CysSer: 0.343 ± 0.019
0.356CysThr: 0.356 ± 0.022
0.563CysVal: 0.563 ± 0.024
0.095CysTrp: 0.095 ± 0.01
0.135CysTyr: 0.135 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
8.022AspAla: 8.022 ± 0.099
0.396AspCys: 0.396 ± 0.02
3.5AspAsp: 3.5 ± 0.083
3.434AspGlu: 3.434 ± 0.065
2.106AspPhe: 2.106 ± 0.05
6.098AspGly: 6.098 ± 0.097
1.274AspHis: 1.274 ± 0.036
2.716AspIle: 2.716 ± 0.057
1.455AspLys: 1.455 ± 0.041
6.236AspLeu: 6.236 ± 0.085
1.25AspMet: 1.25 ± 0.033
1.308AspAsn: 1.308 ± 0.044
3.789AspPro: 3.789 ± 0.069
2.012AspGln: 2.012 ± 0.039
5.059AspArg: 5.059 ± 0.071
2.144AspSer: 2.144 ± 0.049
2.691AspThr: 2.691 ± 0.064
4.322AspVal: 4.322 ± 0.073
1.206AspTrp: 1.206 ± 0.036
1.534AspTyr: 1.534 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
8.984GluAla: 8.984 ± 0.116
0.244GluCys: 0.244 ± 0.018
2.989GluAsp: 2.989 ± 0.068
2.785GluGlu: 2.785 ± 0.066
1.53GluPhe: 1.53 ± 0.038
5.177GluGly: 5.177 ± 0.073
1.02GluHis: 1.02 ± 0.033
2.905GluIle: 2.905 ± 0.061
1.672GluLys: 1.672 ± 0.045
4.918GluLeu: 4.918 ± 0.084
1.328GluMet: 1.328 ± 0.034
1.294GluAsn: 1.294 ± 0.035
2.989GluPro: 2.989 ± 0.061
2.202GluGln: 2.202 ± 0.053
5.131GluArg: 5.131 ± 0.085
2.191GluSer: 2.191 ± 0.041
3.858GluThr: 3.858 ± 0.063
4.173GluVal: 4.173 ± 0.075
0.786GluTrp: 0.786 ± 0.031
0.943GluTyr: 0.943 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
4.501PheAla: 4.501 ± 0.076
0.311PheCys: 0.311 ± 0.017
2.713PheAsp: 2.713 ± 0.054
2.229PheGlu: 2.229 ± 0.051
1.181PhePhe: 1.181 ± 0.04
3.608PheGly: 3.608 ± 0.066
0.682PheHis: 0.682 ± 0.029
1.532PheIle: 1.532 ± 0.049
0.8PheLys: 0.8 ± 0.033
2.85PheLeu: 2.85 ± 0.058
0.764PheMet: 0.764 ± 0.031
1.045PheAsn: 1.045 ± 0.036
1.421PhePro: 1.421 ± 0.036
1.015PheGln: 1.015 ± 0.032
2.269PheArg: 2.269 ± 0.051
1.901PheSer: 1.901 ± 0.042
2.068PheThr: 2.068 ± 0.05
2.549PheVal: 2.549 ± 0.047
0.52PheTrp: 0.52 ± 0.024
0.773PheTyr: 0.773 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
11.256GlyAla: 11.256 ± 0.117
0.739GlyCys: 0.739 ± 0.027
5.379GlyAsp: 5.379 ± 0.114
5.335GlyGlu: 5.335 ± 0.072
3.566GlyPhe: 3.566 ± 0.068
8.848GlyGly: 8.848 ± 0.158
1.669GlyHis: 1.669 ± 0.043
3.182GlyIle: 3.182 ± 0.056
2.732GlyLys: 2.732 ± 0.065
9.72GlyLeu: 9.72 ± 0.11
2.231GlyMet: 2.231 ± 0.046
1.778GlyAsn: 1.778 ± 0.069
4.204GlyPro: 4.204 ± 0.073
3.421GlyGln: 3.421 ± 0.065
7.175GlyArg: 7.175 ± 0.096
4.58GlySer: 4.58 ± 0.087
3.786GlyThr: 3.786 ± 0.094
7.884GlyVal: 7.884 ± 0.092
1.811GlyTrp: 1.811 ± 0.043
2.167GlyTyr: 2.167 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.184HisAla: 2.184 ± 0.048
0.154HisCys: 0.154 ± 0.012
1.104HisAsp: 1.104 ± 0.036
1.004HisGlu: 1.004 ± 0.034
0.609HisPhe: 0.609 ± 0.025
1.842HisGly: 1.842 ± 0.048
0.443HisHis: 0.443 ± 0.026
0.73HisIle: 0.73 ± 0.024
0.36HisLys: 0.36 ± 0.02
1.671HisLeu: 1.671 ± 0.044
0.417HisMet: 0.417 ± 0.02
0.382HisAsn: 0.382 ± 0.02
1.209HisPro: 1.209 ± 0.04
0.516HisGln: 0.516 ± 0.022
1.258HisArg: 1.258 ± 0.04
0.784HisSer: 0.784 ± 0.03
0.769HisThr: 0.769 ± 0.027
1.29HisVal: 1.29 ± 0.039
0.309HisTrp: 0.309 ± 0.017
0.441HisTyr: 0.441 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
6.154IleAla: 6.154 ± 0.081
0.357IleCys: 0.357 ± 0.02
3.321IleAsp: 3.321 ± 0.055
3.271IleGlu: 3.271 ± 0.057
1.32IlePhe: 1.32 ± 0.046
4.474IleGly: 4.474 ± 0.075
0.835IleHis: 0.835 ± 0.027
1.779IleIle: 1.779 ± 0.044
1.083IleLys: 1.083 ± 0.036
3.818IleLeu: 3.818 ± 0.071
0.776IleMet: 0.776 ± 0.028
1.25IleAsn: 1.25 ± 0.035
1.958IlePro: 1.958 ± 0.046
1.247IleGln: 1.247 ± 0.037
3.05IleArg: 3.05 ± 0.055
2.386IleSer: 2.386 ± 0.058
2.51IleThr: 2.51 ± 0.05
3.542IleVal: 3.542 ± 0.058
0.566IleTrp: 0.566 ± 0.024
0.85IleTyr: 0.85 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
4.316LysAla: 4.316 ± 0.079
0.105LysCys: 0.105 ± 0.011
1.445LysAsp: 1.445 ± 0.044
1.105LysGlu: 1.105 ± 0.04
0.756LysPhe: 0.756 ± 0.031
2.387LysGly: 2.387 ± 0.057
0.488LysHis: 0.488 ± 0.024
1.227LysIle: 1.227 ± 0.037
0.98LysLys: 0.98 ± 0.044
2.51LysLeu: 2.51 ± 0.052
0.57LysMet: 0.57 ± 0.024
0.548LysAsn: 0.548 ± 0.025
1.864LysPro: 1.864 ± 0.045
0.75LysGln: 0.75 ± 0.025
2.076LysArg: 2.076 ± 0.055
1.386LysSer: 1.386 ± 0.043
1.725LysThr: 1.725 ± 0.044
2.08LysVal: 2.08 ± 0.055
0.317LysTrp: 0.317 ± 0.02
0.473LysTyr: 0.473 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
13.0LeuAla: 13.0 ± 0.132
0.654LeuCys: 0.654 ± 0.024
6.179LeuAsp: 6.179 ± 0.095
5.436LeuGlu: 5.436 ± 0.072
3.399LeuPhe: 3.399 ± 0.067
8.042LeuGly: 8.042 ± 0.11
1.653LeuHis: 1.653 ± 0.044
5.192LeuIle: 5.192 ± 0.083
3.662LeuLys: 3.662 ± 0.067
8.683LeuLeu: 8.683 ± 0.132
2.358LeuMet: 2.358 ± 0.051
2.953LeuAsn: 2.953 ± 0.06
5.193LeuPro: 5.193 ± 0.088
2.694LeuGln: 2.694 ± 0.049
6.565LeuArg: 6.565 ± 0.097
6.307LeuSer: 6.307 ± 0.077
6.642LeuThr: 6.642 ± 0.085
6.626LeuVal: 6.626 ± 0.085
1.257LeuTrp: 1.257 ± 0.038
2.0LeuTyr: 2.0 ± 0.05
0.0LeuXaa: 0.0 ± 0.0
Met
3.159MetAla: 3.159 ± 0.062
0.111MetCys: 0.111 ± 0.009
1.205MetAsp: 1.205 ± 0.041
1.022MetGlu: 1.022 ± 0.035
0.662MetPhe: 0.662 ± 0.027
1.946MetGly: 1.946 ± 0.043
0.333MetHis: 0.333 ± 0.018
1.187MetIle: 1.187 ± 0.04
0.916MetLys: 0.916 ± 0.028
1.999MetLeu: 1.999 ± 0.05
0.577MetMet: 0.577 ± 0.025
0.629MetAsn: 0.629 ± 0.023
1.211MetPro: 1.211 ± 0.037
0.703MetGln: 0.703 ± 0.028
1.701MetArg: 1.701 ± 0.044
1.522MetSer: 1.522 ± 0.04
2.07MetThr: 2.07 ± 0.049
1.487MetVal: 1.487 ± 0.042
0.186MetTrp: 0.186 ± 0.012
0.247MetTyr: 0.247 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.339AsnAla: 3.339 ± 0.063
0.194AsnCys: 0.194 ± 0.015
1.417AsnAsp: 1.417 ± 0.054
1.072AsnGlu: 1.072 ± 0.039
0.749AsnPhe: 0.749 ± 0.036
2.503AsnGly: 2.503 ± 0.065
0.441AsnHis: 0.441 ± 0.023
1.132AsnIle: 1.132 ± 0.041
0.42AsnLys: 0.42 ± 0.024
2.481AsnLeu: 2.481 ± 0.055
0.528AsnMet: 0.528 ± 0.024
0.629AsnAsn: 0.629 ± 0.029
1.763AsnPro: 1.763 ± 0.041
0.713AsnGln: 0.713 ± 0.03
1.785AsnArg: 1.785 ± 0.042
1.08AsnSer: 1.08 ± 0.037
1.256AsnThr: 1.256 ± 0.037
1.873AsnVal: 1.873 ± 0.057
0.383AsnTrp: 0.383 ± 0.019
0.595AsnTyr: 0.595 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
7.328ProAla: 7.328 ± 0.1
0.285ProCys: 0.285 ± 0.016
4.027ProAsp: 4.027 ± 0.072
4.03ProGlu: 4.03 ± 0.077
1.934ProPhe: 1.934 ± 0.037
5.135ProGly: 5.135 ± 0.061
0.931ProHis: 0.931 ± 0.027
2.124ProIle: 2.124 ± 0.05
1.368ProLys: 1.368 ± 0.039
4.779ProLeu: 4.779 ± 0.079
1.119ProMet: 1.119 ± 0.04
1.437ProAsn: 1.437 ± 0.038
3.131ProPro: 3.131 ± 0.096
1.607ProGln: 1.607 ± 0.04
3.315ProArg: 3.315 ± 0.069
2.82ProSer: 2.82 ± 0.054
2.979ProThr: 2.979 ± 0.056
4.79ProVal: 4.79 ± 0.079
0.8ProTrp: 0.8 ± 0.033
1.093ProTyr: 1.093 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
4.615GlnAla: 4.615 ± 0.075
0.148GlnCys: 0.148 ± 0.013
1.508GlnAsp: 1.508 ± 0.038
1.362GlnGlu: 1.362 ± 0.037
0.936GlnPhe: 0.936 ± 0.032
2.687GlnGly: 2.687 ± 0.049
0.506GlnHis: 0.506 ± 0.021
1.592GlnIle: 1.592 ± 0.045
0.837GlnLys: 0.837 ± 0.03
2.662GlnLeu: 2.662 ± 0.055
0.812GlnMet: 0.812 ± 0.031
0.731GlnAsn: 0.731 ± 0.029
1.93GlnPro: 1.93 ± 0.04
1.115GlnGln: 1.115 ± 0.039
2.355GlnArg: 2.355 ± 0.058
1.702GlnSer: 1.702 ± 0.044
2.167GlnThr: 2.167 ± 0.046
2.3GlnVal: 2.3 ± 0.048
0.416GlnTrp: 0.416 ± 0.02
0.571GlnTyr: 0.571 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
9.191ArgAla: 9.191 ± 0.114
0.455ArgCys: 0.455 ± 0.021
4.381ArgAsp: 4.381 ± 0.072
4.199ArgGlu: 4.199 ± 0.084
3.059ArgPhe: 3.059 ± 0.061
5.429ArgGly: 5.429 ± 0.086
1.26ArgHis: 1.26 ± 0.037
3.79ArgIle: 3.79 ± 0.062
2.081ArgLys: 2.081 ± 0.048
8.85ArgLeu: 8.85 ± 0.126
1.937ArgMet: 1.937 ± 0.046
1.764ArgAsn: 1.764 ± 0.037
4.379ArgPro: 4.379 ± 0.068
2.404ArgGln: 2.404 ± 0.048
6.36ArgArg: 6.36 ± 0.111
3.572ArgSer: 3.572 ± 0.072
3.987ArgThr: 3.987 ± 0.061
5.151ArgVal: 5.151 ± 0.076
1.287ArgTrp: 1.287 ± 0.035
1.794ArgTyr: 1.794 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
6.47SerAla: 6.47 ± 0.09
0.32SerCys: 0.32 ± 0.02
3.034SerAsp: 3.034 ± 0.054
2.563SerGlu: 2.563 ± 0.055
1.775SerPhe: 1.775 ± 0.045
5.368SerGly: 5.368 ± 0.073
0.877SerHis: 0.877 ± 0.03
2.26SerIle: 2.26 ± 0.053
1.259SerLys: 1.259 ± 0.035
5.072SerLeu: 5.072 ± 0.072
1.123SerMet: 1.123 ± 0.03
1.362SerAsn: 1.362 ± 0.045
3.09SerPro: 3.09 ± 0.054
1.556SerGln: 1.556 ± 0.039
3.748SerArg: 3.748 ± 0.074
2.598SerSer: 2.598 ± 0.068
2.756SerThr: 2.756 ± 0.06
3.922SerVal: 3.922 ± 0.063
0.817SerTrp: 0.817 ± 0.031
1.132SerTyr: 1.132 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
7.221ThrAla: 7.221 ± 0.098
0.371ThrCys: 0.371 ± 0.023
3.295ThrAsp: 3.295 ± 0.061
2.72ThrGlu: 2.72 ± 0.053
1.965ThrPhe: 1.965 ± 0.049
5.821ThrGly: 5.821 ± 0.097
0.899ThrHis: 0.899 ± 0.033
2.287ThrIle: 2.287 ± 0.055
1.105ThrLys: 1.105 ± 0.032
6.082ThrLeu: 6.082 ± 0.089
0.87ThrMet: 0.87 ± 0.032
1.272ThrAsn: 1.272 ± 0.043
4.203ThrPro: 4.203 ± 0.064
1.412ThrGln: 1.412 ± 0.039
3.873ThrArg: 3.873 ± 0.071
2.585ThrSer: 2.585 ± 0.059
3.098ThrThr: 3.098 ± 0.073
4.822ThrVal: 4.822 ± 0.078
0.701ThrTrp: 0.701 ± 0.027
1.236ThrTyr: 1.236 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
9.473ValAla: 9.473 ± 0.11
0.647ValCys: 0.647 ± 0.025
4.37ValAsp: 4.37 ± 0.068
5.162ValGlu: 5.162 ± 0.079
2.794ValPhe: 2.794 ± 0.052
6.215ValGly: 6.215 ± 0.092
1.255ValHis: 1.255 ± 0.039
3.913ValIle: 3.913 ± 0.062
2.033ValLys: 2.033 ± 0.05
7.619ValLeu: 7.619 ± 0.091
1.788ValMet: 1.788 ± 0.046
2.009ValAsn: 2.009 ± 0.054
3.129ValPro: 3.129 ± 0.059
2.227ValGln: 2.227 ± 0.053
5.651ValArg: 5.651 ± 0.078
4.586ValSer: 4.586 ± 0.083
4.73ValThr: 4.73 ± 0.078
6.486ValVal: 6.486 ± 0.083
1.159ValTrp: 1.159 ± 0.033
1.325ValTyr: 1.325 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.6TrpAla: 1.6 ± 0.044
0.118TrpCys: 0.118 ± 0.012
0.735TrpAsp: 0.735 ± 0.028
0.643TrpGlu: 0.643 ± 0.027
0.602TrpPhe: 0.602 ± 0.025
1.06TrpGly: 1.06 ± 0.035
0.241TrpHis: 0.241 ± 0.018
0.713TrpIle: 0.713 ± 0.028
0.484TrpLys: 0.484 ± 0.024
1.736TrpLeu: 1.736 ± 0.046
0.412TrpMet: 0.412 ± 0.02
0.451TrpAsn: 0.451 ± 0.022
0.785TrpPro: 0.785 ± 0.03
0.451TrpGln: 0.451 ± 0.022
1.485TrpArg: 1.485 ± 0.052
0.991TrpSer: 0.991 ± 0.033
1.046TrpThr: 1.046 ± 0.039
0.934TrpVal: 0.934 ± 0.032
0.283TrpTrp: 0.283 ± 0.018
0.251TrpTyr: 0.251 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.465TyrAla: 2.465 ± 0.051
0.17TyrCys: 0.17 ± 0.015
1.486TyrAsp: 1.486 ± 0.047
1.256TyrGlu: 1.256 ± 0.035
0.756TyrPhe: 0.756 ± 0.025
2.252TyrGly: 2.252 ± 0.051
0.362TyrHis: 0.362 ± 0.018
0.71TyrIle: 0.71 ± 0.028
0.419TyrLys: 0.419 ± 0.02
1.926TyrLeu: 1.926 ± 0.049
0.409TyrMet: 0.409 ± 0.02
0.518TyrAsn: 0.518 ± 0.027
0.977TyrPro: 0.977 ± 0.031
0.64TyrGln: 0.64 ± 0.028
1.718TyrArg: 1.718 ± 0.047
1.08TyrSer: 1.08 ± 0.037
0.939TyrThr: 0.939 ± 0.033
1.653TyrVal: 1.653 ± 0.049
0.325TyrTrp: 0.325 ± 0.02
0.468TyrTyr: 0.468 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3160 proteins (993302 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski