Tenacibaculum sp. KUL118
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6803 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A640Q3I3|A0A640Q3I3_9FLAO Membrane protein OS=Tenacibaculum sp. KUL118 OX=2480802 GN=KUL118_41580 PE=3 SV=1
MM1 pKa = 7.74 SDD3 pKa = 3.06 SALSIGLFYY12 pKa = 11.16 GSTTCYY18 pKa = 10.67 TEE20 pKa = 3.71 MAAEE24 pKa = 5.15 KK25 pKa = 10.23 IQAQLNSLFDD35 pKa = 3.95 EE36 pKa = 5.26 NIVEE40 pKa = 3.9 LHH42 pKa = 6.22 NIKK45 pKa = 10.46 DD46 pKa = 3.71 VSLSRR51 pKa = 11.84 TADD54 pKa = 3.15 YY55 pKa = 11.03 DD56 pKa = 3.69 IIIFGISTWDD66 pKa = 3.43 FGEE69 pKa = 4.3 LQEE72 pKa = 5.04 DD73 pKa = 4.5 WEE75 pKa = 4.76 SHH77 pKa = 4.89 WEE79 pKa = 4.11 DD80 pKa = 3.59 VHH82 pKa = 9.11 SLDD85 pKa = 5.8 LSGKK89 pKa = 7.97 TIALFGLGDD98 pKa = 3.5 QLGYY102 pKa = 11.04 GEE104 pKa = 4.72 WFQDD108 pKa = 3.69 ALGMLHH114 pKa = 7.44 DD115 pKa = 6.17 AILPSGCNVVGYY127 pKa = 8.9 WPNEE131 pKa = 3.99 GYY133 pKa = 10.9 EE134 pKa = 4.26 FTASKK139 pKa = 11.01 ALTEE143 pKa = 4.52 DD144 pKa = 3.38 RR145 pKa = 11.84 SQFVGLSLDD154 pKa = 5.49 DD155 pKa = 3.93 EE156 pKa = 4.39 NQYY159 pKa = 11.8 DD160 pKa = 3.7 KK161 pKa = 11.34 TDD163 pKa = 3.17 ARR165 pKa = 11.84 IEE167 pKa = 4.07 AWCEE171 pKa = 3.52 QLLVSLAA178 pKa = 3.54
Molecular weight: 19.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.897
IPC_protein 3.884
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.592
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.177
Thurlkill 3.719
EMBOSS 3.795
Sillero 3.999
Patrickios 1.1
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.892
Protein with the highest isoelectric point:
>tr|A0A640PYJ0|A0A640PYJ0_9FLAO TonB-dependent receptor OS=Tenacibaculum sp. KUL118 OX=2480802 GN=KUL118_24030 PE=3 SV=1
MM1 pKa = 7.73 EE2 pKa = 5.53 YY3 pKa = 10.13 IFGFIIQIAGIMLLAKK19 pKa = 10.31 LSWSLLRR26 pKa = 11.84 LLARR30 pKa = 11.84 QSVRR34 pKa = 11.84 PFRR37 pKa = 11.84 FVFTQIKK44 pKa = 10.23 RR45 pKa = 11.84 LFTPTPKK52 pKa = 9.72 RR53 pKa = 11.84 RR54 pKa = 11.84 PMAVPKK60 pKa = 10.52 APGMPRR66 pKa = 11.84 LTSAFYY72 pKa = 10.61 KK73 pKa = 10.48 EE74 pKa = 3.67 PHH76 pKa = 6.1 QYY78 pKa = 11.64 DD79 pKa = 3.46 MALLEE84 pKa = 4.31 IPTYY88 pKa = 10.64 LRR90 pKa = 11.84 RR91 pKa = 11.84 QSSLPSRR98 pKa = 11.84 VEE100 pKa = 3.68 ATVCTEE106 pKa = 3.94 FNN108 pKa = 3.45
Molecular weight: 12.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.94
IPC_protein 10.862
Toseland 10.833
ProMoST 10.657
Dawson 10.935
Bjellqvist 10.701
Wikipedia 11.199
Rodwell 11.067
Grimsley 10.994
Solomon 11.096
Lehninger 11.052
Nozaki 10.818
DTASelect 10.701
Thurlkill 10.833
EMBOSS 11.242
Sillero 10.877
Patrickios 10.818
IPC_peptide 11.096
IPC2_peptide 9.78
IPC2.peptide.svr19 8.332
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6803
0
6803
2271612
40
3581
333.9
37.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.654 ± 0.036
0.831 ± 0.009
5.593 ± 0.021
6.669 ± 0.027
4.646 ± 0.022
6.487 ± 0.028
1.999 ± 0.015
6.905 ± 0.026
6.819 ± 0.042
9.536 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.289 ± 0.014
5.395 ± 0.029
3.588 ± 0.016
3.777 ± 0.021
3.997 ± 0.023
6.666 ± 0.026
5.756 ± 0.025
6.752 ± 0.024
1.112 ± 0.01
3.527 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here