Citrus leprosis virus C (isolate Citrus sinesis/Brazil/Cordeiropolis/2003) (CiLV-C)
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q1KZ58|P29_CILVC P29 OS=Citrus leprosis virus C (isolate Citrus sinesis/Brazil/Cordeiropolis/2003) OX=686950 GN=p29 PE=4 SV=1
MM1 pKa = 7.4 LNWSTIEE8 pKa = 4.21 WDD10 pKa = 4.31 SFWQQHH16 pKa = 6.14 DD17 pKa = 3.98 CGCFTFEE24 pKa = 4.92 CDD26 pKa = 4.87 FITSIDD32 pKa = 4.06 PLVHH36 pKa = 7.38 DD37 pKa = 3.78 YY38 pKa = 11.34 AIYY41 pKa = 10.49 HH42 pKa = 6.08 SLSQKK47 pKa = 9.03 TVLEE51 pKa = 4.03 MLQTHH56 pKa = 6.61 LVAGPDD62 pKa = 3.11 ASEE65 pKa = 4.08 TIRR68 pKa = 11.84 RR69 pKa = 11.84 QVAFLIYY76 pKa = 10.14 DD77 pKa = 3.6 FHH79 pKa = 8.15 RR80 pKa = 11.84 LSCNCDD86 pKa = 2.78 KK87 pKa = 11.24 CYY89 pKa = 11.06 GDD91 pKa = 5.22 CNATTTGRR99 pKa = 11.84 FKK101 pKa = 11.3 VVDD104 pKa = 3.71 RR105 pKa = 11.84 VLNDD109 pKa = 3.93 HH110 pKa = 6.84 IEE112 pKa = 4.01 FGIMRR117 pKa = 11.84 RR118 pKa = 11.84 QDD120 pKa = 4.79 LIPILHH126 pKa = 6.24 NLEE129 pKa = 4.15 TT130 pKa = 4.13
Molecular weight: 15.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.841
IPC2_protein 5.016
IPC_protein 4.965
Toseland 5.016
ProMoST 5.181
Dawson 5.054
Bjellqvist 5.143
Wikipedia 4.991
Rodwell 4.965
Grimsley 4.978
Solomon 5.054
Lehninger 5.016
Nozaki 5.207
DTASelect 5.423
Thurlkill 5.092
EMBOSS 5.105
Sillero 5.27
Patrickios 2.092
IPC_peptide 5.054
IPC2_peptide 5.27
IPC2.peptide.svr19 5.14
Protein with the highest isoelectric point:
>sp|Q1KZ55|MVP_CILVC Putative movement protein OS=Citrus leprosis virus C (isolate Citrus sinesis/Brazil/Cordeiropolis/2003) OX=686950 GN=MP PE=3 SV=1
MM1 pKa = 8.27 DD2 pKa = 4.88 AQLLQANKK10 pKa = 10.18 RR11 pKa = 11.84 LLRR14 pKa = 11.84 RR15 pKa = 11.84 AANVRR20 pKa = 11.84 QRR22 pKa = 11.84 YY23 pKa = 9.26 KK24 pKa = 10.31 MLATEE29 pKa = 4.62 SFVADD34 pKa = 3.82 IKK36 pKa = 11.1 QILLRR41 pKa = 11.84 FIQKK45 pKa = 9.55 PNVIIMYY52 pKa = 10.01 ISVLVLFAAHH62 pKa = 6.95 IDD64 pKa = 4.23 SNTHH68 pKa = 7.67 DD69 pKa = 4.24 ILDD72 pKa = 4.23 DD73 pKa = 4.2 LAAQFPNNTFIEE85 pKa = 4.4 WAKK88 pKa = 11.11 SNFFRR93 pKa = 11.84 ICGALVFIPVIIDD106 pKa = 3.29 TEE108 pKa = 4.4 EE109 pKa = 3.56 KK110 pKa = 9.9 HH111 pKa = 6.61 RR112 pKa = 11.84 NYY114 pKa = 10.28 LALVIFVFLMGFPQRR129 pKa = 11.84 SIMEE133 pKa = 3.88 YY134 pKa = 9.83 FIYY137 pKa = 10.5 SISFHH142 pKa = 7.15 VYY144 pKa = 9.71 AKK146 pKa = 10.31 AKK148 pKa = 10.45 HH149 pKa = 5.03 PVTRR153 pKa = 11.84 IFIIGAAVFSCVMFGIFTNEE173 pKa = 3.88 QLRR176 pKa = 11.84 KK177 pKa = 9.98 LYY179 pKa = 10.94 AEE181 pKa = 4.3 LPKK184 pKa = 10.77 VPTHH188 pKa = 6.06 PVAVNRR194 pKa = 11.84 VEE196 pKa = 4.65 KK197 pKa = 10.21 VANRR201 pKa = 11.84 ASRR204 pKa = 11.84 VSTEE208 pKa = 3.74 GTVNFGG214 pKa = 3.37
Molecular weight: 24.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.167
IPC2_protein 9.165
IPC_protein 9.18
Toseland 9.765
ProMoST 9.531
Dawson 10.014
Bjellqvist 9.721
Wikipedia 10.204
Rodwell 10.321
Grimsley 10.101
Solomon 10.058
Lehninger 10.014
Nozaki 9.809
DTASelect 9.706
Thurlkill 9.853
EMBOSS 10.189
Sillero 9.94
Patrickios 7.614
IPC_peptide 10.058
IPC2_peptide 8.419
IPC2.peptide.svr19 8.145
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3953
130
2512
658.8
74.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.034 ± 0.966
2.707 ± 0.522
6.704 ± 0.7
5.186 ± 0.239
5.667 ± 0.608
4.882 ± 0.511
2.757 ± 0.225
6.527 ± 0.599
6.046 ± 0.561
9.006 ± 0.431
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.998 ± 0.185
4.376 ± 0.462
3.693 ± 0.598
2.808 ± 0.924
5.515 ± 0.611
8.297 ± 0.785
5.237 ± 0.443
8.677 ± 0.534
0.582 ± 0.139
4.301 ± 0.799
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here