Streptomyces oceani
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4624 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E7KH84|A0A1E7KH84_9ACTN ABC transporter permease OS=Streptomyces oceani OX=1075402 GN=AN216_12235 PE=4 SV=1
MM1 pKa = 7.02 STGNEE6 pKa = 3.93 ALEE9 pKa = 3.98 VWIDD13 pKa = 3.41 QDD15 pKa = 3.99 LCTGDD20 pKa = 5.01 GICAQYY26 pKa = 10.9 APEE29 pKa = 4.23 VFEE32 pKa = 5.9 LDD34 pKa = 3.05 IDD36 pKa = 3.63 GLAYY40 pKa = 10.39 VKK42 pKa = 10.61 SAEE45 pKa = 4.68 DD46 pKa = 3.36 EE47 pKa = 4.24 LLQQEE52 pKa = 5.05 GATTPVPLPLLQDD65 pKa = 3.66 VVDD68 pKa = 4.35 SAKK71 pKa = 10.25 EE72 pKa = 3.92 CPGDD76 pKa = 4.18 CIHH79 pKa = 6.36 VRR81 pKa = 11.84 RR82 pKa = 11.84 VSDD85 pKa = 3.22 RR86 pKa = 11.84 VEE88 pKa = 3.77 VHH90 pKa = 6.69 GPDD93 pKa = 3.9 AEE95 pKa = 4.16
Molecular weight: 10.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.859
IPC_protein 3.808
Toseland 3.605
ProMoST 3.935
Dawson 3.795
Bjellqvist 3.999
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.668
EMBOSS 3.732
Sillero 3.923
Patrickios 1.875
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A1E7KCQ7|A0A1E7KCQ7_9ACTN Bacterioferritin OS=Streptomyces oceani OX=1075402 GN=AN216_16620 PE=3 SV=1
MM1 pKa = 7.7 PLSEE5 pKa = 4.33 NEE7 pKa = 3.9 QRR9 pKa = 11.84 MLEE12 pKa = 4.04 QMEE15 pKa = 4.17 RR16 pKa = 11.84 ALYY19 pKa = 10.89 AEE21 pKa = 4.71 DD22 pKa = 4.13 PKK24 pKa = 11.08 FATALEE30 pKa = 4.44 GSEE33 pKa = 4.72 LRR35 pKa = 11.84 THH37 pKa = 5.14 TRR39 pKa = 11.84 KK40 pKa = 9.63 RR41 pKa = 11.84 AYY43 pKa = 9.7 QAVAGFLVGIALLMAGMVAQQIWISVVGFLVMLGCAVLAVTSWRR87 pKa = 11.84 KK88 pKa = 9.14 SPKK91 pKa = 10.15 AGEE94 pKa = 4.08 EE95 pKa = 4.02 QAGSGAAAGGRR106 pKa = 11.84 ASGGAAQQRR115 pKa = 11.84 RR116 pKa = 11.84 PRR118 pKa = 11.84 RR119 pKa = 11.84 SMMNRR124 pKa = 11.84 IEE126 pKa = 4.44 EE127 pKa = 3.94 RR128 pKa = 11.84 WQRR131 pKa = 11.84 RR132 pKa = 11.84 RR133 pKa = 11.84 DD134 pKa = 3.49 EE135 pKa = 4.31 RR136 pKa = 11.84 GG137 pKa = 2.85
Molecular weight: 15.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.458
IPC_protein 10.438
Toseland 10.643
ProMoST 10.438
Dawson 10.73
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 10.774
Grimsley 10.774
Solomon 10.921
Lehninger 10.877
Nozaki 10.643
DTASelect 10.482
Thurlkill 10.643
EMBOSS 11.067
Sillero 10.672
Patrickios 10.54
IPC_peptide 10.921
IPC2_peptide 9.56
IPC2.peptide.svr19 8.709
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4621
3
4624
1511313
23
9273
326.8
35.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.951 ± 0.048
0.818 ± 0.01
6.028 ± 0.025
6.491 ± 0.031
2.68 ± 0.018
9.507 ± 0.038
2.322 ± 0.017
2.896 ± 0.027
1.93 ± 0.03
10.434 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.784 ± 0.016
1.681 ± 0.016
6.025 ± 0.031
2.885 ± 0.021
8.356 ± 0.04
5.358 ± 0.026
5.92 ± 0.022
8.415 ± 0.036
1.463 ± 0.018
2.052 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here