Seonamhaeicola sp. S2-3
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3101 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U7DT73|A0A1U7DT73_9FLAO Cytochrome oxidase maturation protein cbb3-type OS=Seonamhaeicola sp. S2-3 OX=1936081 GN=BWZ22_10905 PE=4 SV=1
MM1 pKa = 7.8 KK2 pKa = 10.03 IFFIGTIIMFFMFSCSDD19 pKa = 4.05 DD20 pKa = 3.28 IKK22 pKa = 10.77 SYY24 pKa = 10.77 PVGSDD29 pKa = 4.55 FIDD32 pKa = 3.44 NDD34 pKa = 3.62 VNLKK38 pKa = 10.47 VLDD41 pKa = 3.99 TFTINAGTFKK51 pKa = 10.63 LDD53 pKa = 3.43 SLITSSTSRR62 pKa = 11.84 ILLGSVKK69 pKa = 10.5 DD70 pKa = 4.27 DD71 pKa = 3.42 YY72 pKa = 11.46 FGNLTAQSYY81 pKa = 7.68 FQVVNSDD88 pKa = 3.61 FSISSDD94 pKa = 3.11 AVYY97 pKa = 10.93 DD98 pKa = 4.31 SIGLVLNYY106 pKa = 10.68 DD107 pKa = 3.24 NYY109 pKa = 11.31 YY110 pKa = 10.7 YY111 pKa = 11.4 GDD113 pKa = 3.51 TTRR116 pKa = 11.84 VQTYY120 pKa = 9.11 KK121 pKa = 9.88 VHH123 pKa = 7.08 RR124 pKa = 11.84 LLEE127 pKa = 4.28 YY128 pKa = 10.64 FEE130 pKa = 5.13 PPEE133 pKa = 6.07 DD134 pKa = 4.95 DD135 pKa = 3.67 DD136 pKa = 5.43 AFYY139 pKa = 11.25 NVSKK143 pKa = 11.04 LEE145 pKa = 3.9 YY146 pKa = 10.24 DD147 pKa = 3.79 EE148 pKa = 5.69 EE149 pKa = 4.48 PLGEE153 pKa = 4.36 FTFSPRR159 pKa = 11.84 PTSTSDD165 pKa = 3.32 SIYY168 pKa = 10.69 IPIDD172 pKa = 3.36 KK173 pKa = 10.74 DD174 pKa = 3.27 LGEE177 pKa = 4.64 EE178 pKa = 3.94 IFNKK182 pKa = 9.75 IRR184 pKa = 11.84 DD185 pKa = 3.64 NDD187 pKa = 3.69 INSTDD192 pKa = 4.42 DD193 pKa = 4.25 FIQYY197 pKa = 10.12 FYY199 pKa = 10.93 GLTVIPDD206 pKa = 3.73 TLVDD210 pKa = 4.09 SNVLGFTYY218 pKa = 10.21 TSSSDD223 pKa = 3.19 IEE225 pKa = 4.58 NNSGMRR231 pKa = 11.84 IFYY234 pKa = 10.18 TEE236 pKa = 5.25 DD237 pKa = 3.35 NDD239 pKa = 5.29 DD240 pKa = 3.81 TNEE243 pKa = 4.55 DD244 pKa = 3.56 NDD246 pKa = 4.07 HH247 pKa = 6.67 VINFYY252 pKa = 10.62 IPSSDD257 pKa = 3.35 KK258 pKa = 10.89 QFNTIKK264 pKa = 10.62 NNLSGTVIEE273 pKa = 4.91 NLDD276 pKa = 3.82 DD277 pKa = 3.81 QEE279 pKa = 4.47 TTIDD283 pKa = 5.17 SYY285 pKa = 11.68 DD286 pKa = 3.43 TDD288 pKa = 3.42 QLIFAQGGIGVSARR302 pKa = 11.84 IEE304 pKa = 4.23 MPTLKK309 pKa = 10.39 YY310 pKa = 10.77 LKK312 pKa = 9.04 TLSDD316 pKa = 3.61 EE317 pKa = 4.4 STSLSAEE324 pKa = 4.02 LTFAPLLNSYY334 pKa = 11.16 DD335 pKa = 3.68 EE336 pKa = 4.3 TRR338 pKa = 11.84 PLQDD342 pKa = 2.89 SLAVYY347 pKa = 10.14 IVDD350 pKa = 3.34 NKK352 pKa = 10.5 NRR354 pKa = 11.84 IVSQLTDD361 pKa = 3.19 ADD363 pKa = 4.4 ANTSYY368 pKa = 11.39 AILNQDD374 pKa = 2.44 SDD376 pKa = 4.18 EE377 pKa = 4.67 FNEE380 pKa = 4.14 STYY383 pKa = 11.37 YY384 pKa = 10.64 SIDD387 pKa = 2.97 MSGFVEE393 pKa = 4.82 TIFNSEE399 pKa = 3.48 TDD401 pKa = 3.5 LNYY404 pKa = 10.89 AIMIQFIDD412 pKa = 3.59 YY413 pKa = 10.15 NKK415 pKa = 9.72 TVDD418 pKa = 4.17 SVIIDD423 pKa = 4.58 DD424 pKa = 5.8 LNEE427 pKa = 4.58 DD428 pKa = 4.13 DD429 pKa = 5.72 NNIKK433 pKa = 10.79 LSVTYY438 pKa = 10.53 LEE440 pKa = 4.62 YY441 pKa = 11.1
Molecular weight: 50.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.681
IPC_protein 3.732
Toseland 3.49
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.541
EMBOSS 3.681
Sillero 3.846
Patrickios 0.846
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A1U7DTE0|A0A1U7DTE0_9FLAO Uncharacterized protein OS=Seonamhaeicola sp. S2-3 OX=1936081 GN=BWZ22_10815 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 7.97 KK42 pKa = 10.72 LSVSSEE48 pKa = 3.76 ARR50 pKa = 11.84 HH51 pKa = 5.78 KK52 pKa = 10.72 KK53 pKa = 9.83
Molecular weight: 6.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3101
0
3101
1092078
26
2404
352.2
39.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.155 ± 0.039
0.762 ± 0.015
5.453 ± 0.035
6.464 ± 0.038
5.222 ± 0.035
6.14 ± 0.039
1.796 ± 0.02
8.078 ± 0.042
8.345 ± 0.055
9.251 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.009 ± 0.022
6.858 ± 0.05
3.337 ± 0.026
3.216 ± 0.023
3.186 ± 0.03
6.402 ± 0.037
5.88 ± 0.049
6.188 ± 0.033
1.046 ± 0.015
4.212 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here