Chitinimonas sp. BJB300
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4049 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2G3K9J4|A0A2G3K9J4_9BURK Uncharacterized protein OS=Chitinimonas sp. BJB300 OX=1559339 GN=CSQ89_00600 PE=4 SV=1
MM1 pKa = 6.72 QTLCIQIQPDD11 pKa = 3.24 RR12 pKa = 11.84 VADD15 pKa = 3.93 FDD17 pKa = 4.22 EE18 pKa = 4.48 ATVLATCQGLRR29 pKa = 11.84 VFKK32 pKa = 10.25 PLIAAFQMEE41 pKa = 4.45 QGEE44 pKa = 5.25 DD45 pKa = 3.41 DD46 pKa = 4.51 GPWINLSFEE55 pKa = 4.46 TEE57 pKa = 3.73 HH58 pKa = 7.28 PARR61 pKa = 11.84 VWPLLQAAFYY71 pKa = 10.68 DD72 pKa = 4.11 AGPLGDD78 pKa = 3.79 VMRR81 pKa = 11.84 ASSMAMCSGEE91 pKa = 4.65 AGWDD95 pKa = 3.61 DD96 pKa = 4.18 YY97 pKa = 12.06 LLLYY101 pKa = 10.51 HH102 pKa = 6.91 YY103 pKa = 10.7 DD104 pKa = 3.34 QDD106 pKa = 5.34 LEE108 pKa = 4.53 LDD110 pKa = 3.88 SLDD113 pKa = 4.27 TPGTTANN120 pKa = 3.79
Molecular weight: 13.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.897
Patrickios 0.896
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A2G3K591|A0A2G3K591_9BURK ABC transporter OS=Chitinimonas sp. BJB300 OX=1559339 GN=CSQ89_08770 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.6 QPSVIRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.64 RR14 pKa = 11.84 THH16 pKa = 5.95 GFLVRR21 pKa = 11.84 SRR23 pKa = 11.84 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.11 GRR39 pKa = 11.84 HH40 pKa = 4.94 SLSVV44 pKa = 3.04
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.428
IPC2_protein 11.111
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.281
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.018
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.094
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4049
0
4049
1312742
26
5314
324.2
35.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.18 ± 0.054
0.923 ± 0.015
5.381 ± 0.029
5.448 ± 0.036
3.553 ± 0.024
7.71 ± 0.048
2.186 ± 0.021
5.009 ± 0.029
4.153 ± 0.03
10.996 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.382 ± 0.02
3.462 ± 0.035
4.734 ± 0.029
4.39 ± 0.027
6.102 ± 0.034
5.806 ± 0.033
5.474 ± 0.045
7.063 ± 0.032
1.383 ± 0.017
2.665 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here