Enterobacteria phage BP-4795

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Marienburgvirus; Enterobacteria virus BP4795

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6H9Y7|Q6H9Y7_9CAUD Putative Kil protein OS=Enterobacteria phage BP-4795 OX=196242 GN=kil PE=4 SV=1
MM1 pKa = 7.47SNIKK5 pKa = 10.19KK6 pKa = 10.63YY7 pKa = 10.7IIDD10 pKa = 4.21YY11 pKa = 9.73DD12 pKa = 3.52WKK14 pKa = 10.79ASIEE18 pKa = 3.99IEE20 pKa = 3.8IDD22 pKa = 3.1HH23 pKa = 7.35DD24 pKa = 4.32VMTEE28 pKa = 3.99EE29 pKa = 5.57KK30 pKa = 10.35LHH32 pKa = 6.08QINNFWSDD40 pKa = 2.68SEE42 pKa = 4.22YY43 pKa = 11.07RR44 pKa = 11.84LNKK47 pKa = 9.52HH48 pKa = 5.94GSVLNAVLIMLAQHH62 pKa = 6.9ALLIAISSDD71 pKa = 2.92LNAYY75 pKa = 9.62GVVCEE80 pKa = 4.87FDD82 pKa = 3.48WNDD85 pKa = 3.26GNGQEE90 pKa = 5.08GWPSMDD96 pKa = 2.65GSEE99 pKa = 4.97GIRR102 pKa = 11.84ITDD105 pKa = 3.36IDD107 pKa = 3.67TSGIFDD113 pKa = 4.38SDD115 pKa = 3.93DD116 pKa = 3.52MTIKK120 pKa = 10.65AAA122 pKa = 3.9

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6H9Y5|Q6H9Y5_9CAUD Uncharacterized protein OS=Enterobacteria phage BP-4795 OX=196242 PE=4 SV=1
MM1 pKa = 6.97ITRR4 pKa = 11.84PEE6 pKa = 3.93LVARR10 pKa = 11.84LYY12 pKa = 11.25SPLKK16 pKa = 9.67FIRR19 pKa = 11.84RR20 pKa = 11.84FHH22 pKa = 4.7MTRR25 pKa = 11.84RR26 pKa = 11.84TQFKK30 pKa = 9.33GNSRR34 pKa = 11.84SRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84EE39 pKa = 3.62RR40 pKa = 11.84LKK42 pKa = 11.17AKK44 pKa = 10.38ALANGVLARR53 pKa = 11.84EE54 pKa = 4.21EE55 pKa = 4.75AISSEE60 pKa = 4.23VLHH63 pKa = 6.83RR64 pKa = 11.84PTLSRR69 pKa = 11.84AQIQAKK75 pKa = 8.13GTHH78 pKa = 5.26EE79 pKa = 4.15TPDD82 pKa = 3.89RR83 pKa = 11.84IEE85 pKa = 3.98DD86 pKa = 3.56AKK88 pKa = 10.63PIKK91 pKa = 10.49FMAQDD96 pKa = 3.82VIWQQEE102 pKa = 4.17EE103 pKa = 4.06YY104 pKa = 10.57RR105 pKa = 11.84RR106 pKa = 11.84NLEE109 pKa = 3.59RR110 pKa = 11.84AAIVYY115 pKa = 10.58ANEE118 pKa = 4.32FGHH121 pKa = 6.02KK122 pKa = 9.59QPEE125 pKa = 4.48TGVCLPNVALYY136 pKa = 10.32AAGYY140 pKa = 9.13RR141 pKa = 11.84KK142 pKa = 9.93SKK144 pKa = 10.61QLTARR149 pKa = 3.76

Molecular weight: 17.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

17443

44

1158

205.2

22.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.626 ± 0.482

1.342 ± 0.15

5.452 ± 0.248

6.455 ± 0.298

3.199 ± 0.156

7.252 ± 0.284

1.932 ± 0.157

5.223 ± 0.184

5.337 ± 0.25

8.038 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.694 ± 0.166

4.26 ± 0.174

4.283 ± 0.274

4.426 ± 0.256

6.748 ± 0.297

6.65 ± 0.233

5.951 ± 0.312

6.702 ± 0.28

1.743 ± 0.119

2.689 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski