Amino acid dipepetide frequency for Enterobacteria phage BP-4795

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.779AlaAla: 10.779 ± 1.346
1.147AlaCys: 1.147 ± 0.257
5.16AlaAsp: 5.16 ± 0.603
8.371AlaGlu: 8.371 ± 0.788
2.752AlaPhe: 2.752 ± 0.49
8.199AlaGly: 8.199 ± 0.716
1.605AlaHis: 1.605 ± 0.316
5.332AlaIle: 5.332 ± 0.492
4.128AlaLys: 4.128 ± 0.682
8.313AlaLeu: 8.313 ± 0.801
2.408AlaMet: 2.408 ± 0.409
3.096AlaAsn: 3.096 ± 0.378
2.408AlaPro: 2.408 ± 0.361
3.841AlaGln: 3.841 ± 0.506
6.364AlaArg: 6.364 ± 0.756
7.109AlaSer: 7.109 ± 0.67
4.931AlaThr: 4.931 ± 0.551
6.421AlaVal: 6.421 ± 1.018
1.777AlaTrp: 1.777 ± 0.306
2.121AlaTyr: 2.121 ± 0.345
0.0AlaXaa: 0.0 ± 0.0
Cys
0.803CysAla: 0.803 ± 0.233
0.459CysCys: 0.459 ± 0.141
0.688CysAsp: 0.688 ± 0.213
0.573CysGlu: 0.573 ± 0.194
0.287CysPhe: 0.287 ± 0.135
1.147CysGly: 1.147 ± 0.26
0.516CysHis: 0.516 ± 0.212
0.631CysIle: 0.631 ± 0.199
0.344CysLys: 0.344 ± 0.145
0.688CysLeu: 0.688 ± 0.196
0.516CysMet: 0.516 ± 0.176
0.401CysAsn: 0.401 ± 0.135
0.459CysPro: 0.459 ± 0.162
0.401CysGln: 0.401 ± 0.139
1.433CysArg: 1.433 ± 0.291
1.204CysSer: 1.204 ± 0.233
1.204CysThr: 1.204 ± 0.22
1.032CysVal: 1.032 ± 0.22
0.229CysTrp: 0.229 ± 0.105
0.401CysTyr: 0.401 ± 0.15
0.0CysXaa: 0.0 ± 0.0
Asp
5.16AspAla: 5.16 ± 0.646
0.344AspCys: 0.344 ± 0.14
3.899AspAsp: 3.899 ± 0.577
3.841AspGlu: 3.841 ± 0.462
1.892AspPhe: 1.892 ± 0.366
5.332AspGly: 5.332 ± 0.747
0.573AspHis: 0.573 ± 0.172
3.669AspIle: 3.669 ± 0.417
3.153AspLys: 3.153 ± 0.468
3.956AspLeu: 3.956 ± 0.56
1.949AspMet: 1.949 ± 0.358
2.179AspAsn: 2.179 ± 0.309
2.236AspPro: 2.236 ± 0.424
1.376AspGln: 1.376 ± 0.287
3.096AspArg: 3.096 ± 0.4
2.867AspSer: 2.867 ± 0.43
2.58AspThr: 2.58 ± 0.483
4.013AspVal: 4.013 ± 0.758
1.147AspTrp: 1.147 ± 0.282
1.261AspTyr: 1.261 ± 0.34
0.0AspXaa: 0.0 ± 0.0
Glu
7.224GluAla: 7.224 ± 0.731
0.917GluCys: 0.917 ± 0.257
2.58GluAsp: 2.58 ± 0.293
3.612GluGlu: 3.612 ± 0.565
2.351GluPhe: 2.351 ± 0.363
4.071GluGly: 4.071 ± 0.43
1.261GluHis: 1.261 ± 0.312
3.727GluIle: 3.727 ± 0.367
3.44GluLys: 3.44 ± 0.445
5.848GluLeu: 5.848 ± 0.674
1.777GluMet: 1.777 ± 0.288
3.44GluAsn: 3.44 ± 0.403
2.064GluPro: 2.064 ± 0.36
3.956GluGln: 3.956 ± 0.546
4.3GluArg: 4.3 ± 0.597
3.784GluSer: 3.784 ± 0.464
3.325GluThr: 3.325 ± 0.464
3.383GluVal: 3.383 ± 0.465
1.204GluTrp: 1.204 ± 0.246
2.293GluTyr: 2.293 ± 0.36
0.0GluXaa: 0.0 ± 0.0
Phe
2.637PheAla: 2.637 ± 0.427
0.516PheCys: 0.516 ± 0.184
1.491PheAsp: 1.491 ± 0.295
1.548PheGlu: 1.548 ± 0.32
1.032PhePhe: 1.032 ± 0.26
2.695PheGly: 2.695 ± 0.376
0.631PheHis: 0.631 ± 0.188
1.433PheIle: 1.433 ± 0.319
1.376PheLys: 1.376 ± 0.313
1.777PheLeu: 1.777 ± 0.303
0.745PheMet: 0.745 ± 0.163
1.319PheAsn: 1.319 ± 0.249
1.376PhePro: 1.376 ± 0.284
0.745PheGln: 0.745 ± 0.171
2.809PheArg: 2.809 ± 0.409
3.383PheSer: 3.383 ± 0.516
2.351PheThr: 2.351 ± 0.479
2.351PheVal: 2.351 ± 0.394
0.631PheTrp: 0.631 ± 0.172
1.147PheTyr: 1.147 ± 0.221
0.0PheXaa: 0.0 ± 0.0
Gly
5.561GlyAla: 5.561 ± 0.609
0.86GlyCys: 0.86 ± 0.235
4.357GlyAsp: 4.357 ± 0.469
4.472GlyGlu: 4.472 ± 0.592
2.695GlyPhe: 2.695 ± 0.344
5.16GlyGly: 5.16 ± 0.71
1.147GlyHis: 1.147 ± 0.23
3.956GlyIle: 3.956 ± 0.489
4.816GlyLys: 4.816 ± 0.599
6.077GlyLeu: 6.077 ± 0.53
2.637GlyMet: 2.637 ± 0.4
3.325GlyAsn: 3.325 ± 0.325
2.637GlyPro: 2.637 ± 1.054
2.981GlyGln: 2.981 ± 0.487
4.3GlyArg: 4.3 ± 0.624
4.701GlySer: 4.701 ± 0.546
3.612GlyThr: 3.612 ± 0.534
5.389GlyVal: 5.389 ± 0.455
1.72GlyTrp: 1.72 ± 0.29
2.064GlyTyr: 2.064 ± 0.356
0.0GlyXaa: 0.0 ± 0.0
His
1.433HisAla: 1.433 ± 0.292
0.401HisCys: 0.401 ± 0.162
1.204HisAsp: 1.204 ± 0.257
0.975HisGlu: 0.975 ± 0.216
0.459HisPhe: 0.459 ± 0.161
1.72HisGly: 1.72 ± 0.289
0.86HisHis: 0.86 ± 0.249
1.433HisIle: 1.433 ± 0.351
1.147HisLys: 1.147 ± 0.262
1.663HisLeu: 1.663 ± 0.354
0.172HisMet: 0.172 ± 0.1
1.147HisAsn: 1.147 ± 0.22
1.089HisPro: 1.089 ± 0.258
0.803HisGln: 0.803 ± 0.193
1.089HisArg: 1.089 ± 0.253
1.147HisSer: 1.147 ± 0.3
0.745HisThr: 0.745 ± 0.197
0.631HisVal: 0.631 ± 0.185
0.229HisTrp: 0.229 ± 0.103
0.975HisTyr: 0.975 ± 0.236
0.0HisXaa: 0.0 ± 0.0
Ile
4.587IleAla: 4.587 ± 0.489
0.803IleCys: 0.803 ± 0.182
3.555IleAsp: 3.555 ± 0.472
3.039IleGlu: 3.039 ± 0.526
1.319IlePhe: 1.319 ± 0.328
3.039IleGly: 3.039 ± 0.331
1.089IleHis: 1.089 ± 0.239
2.523IleIle: 2.523 ± 0.316
3.268IleLys: 3.268 ± 0.513
4.128IleLeu: 4.128 ± 0.479
0.803IleMet: 0.803 ± 0.237
2.465IleAsn: 2.465 ± 0.396
2.465IlePro: 2.465 ± 0.62
2.007IleGln: 2.007 ± 0.287
4.415IleArg: 4.415 ± 0.546
4.128IleSer: 4.128 ± 0.589
4.357IleThr: 4.357 ± 0.485
2.523IleVal: 2.523 ± 0.381
0.631IleTrp: 0.631 ± 0.205
1.089IleTyr: 1.089 ± 0.341
0.0IleXaa: 0.0 ± 0.0
Lys
5.676LysAla: 5.676 ± 0.605
0.631LysCys: 0.631 ± 0.194
2.236LysAsp: 2.236 ± 0.312
3.325LysGlu: 3.325 ± 0.477
0.975LysPhe: 0.975 ± 0.2
3.612LysGly: 3.612 ± 0.525
0.803LysHis: 0.803 ± 0.185
2.695LysIle: 2.695 ± 0.346
3.841LysLys: 3.841 ± 0.644
4.3LysLeu: 4.3 ± 0.54
1.835LysMet: 1.835 ± 0.372
2.523LysAsn: 2.523 ± 0.387
2.523LysPro: 2.523 ± 0.347
2.924LysGln: 2.924 ± 0.361
2.752LysArg: 2.752 ± 0.415
3.612LysSer: 3.612 ± 0.481
3.727LysThr: 3.727 ± 0.487
3.039LysVal: 3.039 ± 0.568
0.975LysTrp: 0.975 ± 0.243
1.376LysTyr: 1.376 ± 0.289
0.0LysXaa: 0.0 ± 0.0
Leu
9.689LeuAla: 9.689 ± 0.782
1.433LeuCys: 1.433 ± 0.259
3.612LeuAsp: 3.612 ± 0.474
4.529LeuGlu: 4.529 ± 0.626
2.064LeuPhe: 2.064 ± 0.316
4.071LeuGly: 4.071 ± 0.583
1.605LeuHis: 1.605 ± 0.312
3.956LeuIle: 3.956 ± 0.544
5.103LeuLys: 5.103 ± 0.519
6.249LeuLeu: 6.249 ± 0.585
2.408LeuMet: 2.408 ± 0.443
2.924LeuAsn: 2.924 ± 0.49
3.784LeuPro: 3.784 ± 0.431
3.669LeuGln: 3.669 ± 0.535
6.249LeuArg: 6.249 ± 0.607
5.389LeuSer: 5.389 ± 0.505
5.791LeuThr: 5.791 ± 0.529
4.185LeuVal: 4.185 ± 0.544
1.835LeuTrp: 1.835 ± 0.285
1.835LeuTyr: 1.835 ± 0.323
0.0LeuXaa: 0.0 ± 0.0
Met
2.752MetAla: 2.752 ± 0.439
0.287MetCys: 0.287 ± 0.13
1.261MetAsp: 1.261 ± 0.302
1.147MetGlu: 1.147 ± 0.23
0.688MetPhe: 0.688 ± 0.199
1.089MetGly: 1.089 ± 0.25
0.115MetHis: 0.115 ± 0.087
1.319MetIle: 1.319 ± 0.299
1.777MetLys: 1.777 ± 0.343
2.981MetLeu: 2.981 ± 0.511
0.688MetMet: 0.688 ± 0.175
1.433MetAsn: 1.433 ± 0.238
1.261MetPro: 1.261 ± 0.243
1.605MetGln: 1.605 ± 0.327
1.663MetArg: 1.663 ± 0.332
2.064MetSer: 2.064 ± 0.339
2.695MetThr: 2.695 ± 0.358
1.548MetVal: 1.548 ± 0.4
0.344MetTrp: 0.344 ± 0.153
0.229MetTyr: 0.229 ± 0.113
0.0MetXaa: 0.0 ± 0.0
Asn
4.644AsnAla: 4.644 ± 0.573
0.459AsnCys: 0.459 ± 0.155
2.351AsnAsp: 2.351 ± 0.425
2.58AsnGlu: 2.58 ± 0.431
1.204AsnPhe: 1.204 ± 0.342
3.727AsnGly: 3.727 ± 0.514
1.032AsnHis: 1.032 ± 0.244
2.637AsnIle: 2.637 ± 0.345
1.949AsnLys: 1.949 ± 0.348
2.637AsnLeu: 2.637 ± 0.335
0.975AsnMet: 0.975 ± 0.278
2.293AsnAsn: 2.293 ± 0.385
2.351AsnPro: 2.351 ± 0.329
1.835AsnGln: 1.835 ± 0.346
2.867AsnArg: 2.867 ± 0.458
2.523AsnSer: 2.523 ± 0.385
2.236AsnThr: 2.236 ± 0.296
2.58AsnVal: 2.58 ± 0.366
0.573AsnTrp: 0.573 ± 0.149
1.147AsnTyr: 1.147 ± 0.223
0.0AsnXaa: 0.0 ± 0.0
Pro
3.612ProAla: 3.612 ± 0.62
0.459ProCys: 0.459 ± 0.136
3.841ProAsp: 3.841 ± 0.536
4.415ProGlu: 4.415 ± 0.48
1.319ProPhe: 1.319 ± 0.313
3.153ProGly: 3.153 ± 0.509
0.975ProHis: 0.975 ± 0.283
1.204ProIle: 1.204 ± 0.266
1.892ProLys: 1.892 ± 0.449
2.58ProLeu: 2.58 ± 0.316
0.917ProMet: 0.917 ± 0.336
1.835ProAsn: 1.835 ± 0.354
2.121ProPro: 2.121 ± 0.518
2.179ProGln: 2.179 ± 0.434
1.949ProArg: 1.949 ± 0.475
2.867ProSer: 2.867 ± 0.296
1.548ProThr: 1.548 ± 0.256
4.357ProVal: 4.357 ± 0.701
0.688ProTrp: 0.688 ± 0.18
0.917ProTyr: 0.917 ± 0.179
0.0ProXaa: 0.0 ± 0.0
Gln
3.956GlnAla: 3.956 ± 0.619
0.86GlnCys: 0.86 ± 0.177
2.064GlnAsp: 2.064 ± 0.276
2.695GlnGlu: 2.695 ± 0.528
1.835GlnPhe: 1.835 ± 0.288
2.752GlnGly: 2.752 ± 0.402
1.147GlnHis: 1.147 ± 0.215
2.867GlnIle: 2.867 ± 0.406
2.752GlnLys: 2.752 ± 0.453
2.809GlnLeu: 2.809 ± 0.39
1.204GlnMet: 1.204 ± 0.383
1.605GlnAsn: 1.605 ± 0.437
1.835GlnPro: 1.835 ± 0.396
2.351GlnGln: 2.351 ± 0.467
3.497GlnArg: 3.497 ± 0.555
2.752GlnSer: 2.752 ± 0.436
2.465GlnThr: 2.465 ± 0.392
2.408GlnVal: 2.408 ± 0.417
0.688GlnTrp: 0.688 ± 0.199
1.72GlnTyr: 1.72 ± 0.288
0.0GlnXaa: 0.0 ± 0.0
Arg
4.759ArgAla: 4.759 ± 0.575
0.86ArgCys: 0.86 ± 0.25
4.415ArgAsp: 4.415 ± 0.866
5.676ArgGlu: 5.676 ± 0.634
2.351ArgPhe: 2.351 ± 0.36
3.784ArgGly: 3.784 ± 0.468
1.663ArgHis: 1.663 ± 0.289
3.325ArgIle: 3.325 ± 0.454
3.784ArgLys: 3.784 ± 0.548
6.593ArgLeu: 6.593 ± 0.677
2.007ArgMet: 2.007 ± 0.289
3.211ArgAsn: 3.211 ± 0.433
2.58ArgPro: 2.58 ± 0.444
3.325ArgGln: 3.325 ± 0.557
5.733ArgArg: 5.733 ± 0.751
2.809ArgSer: 2.809 ± 0.456
3.039ArgThr: 3.039 ± 0.353
4.759ArgVal: 4.759 ± 0.548
1.147ArgTrp: 1.147 ± 0.267
1.663ArgTyr: 1.663 ± 0.345
0.0ArgXaa: 0.0 ± 0.0
Ser
7.052SerAla: 7.052 ± 0.933
0.86SerCys: 0.86 ± 0.236
3.383SerAsp: 3.383 ± 0.511
4.587SerGlu: 4.587 ± 0.507
2.064SerPhe: 2.064 ± 0.296
6.307SerGly: 6.307 ± 0.742
1.089SerHis: 1.089 ± 0.369
2.867SerIle: 2.867 ± 0.36
2.523SerLys: 2.523 ± 0.392
4.816SerLeu: 4.816 ± 0.543
2.351SerMet: 2.351 ± 0.419
2.867SerAsn: 2.867 ± 0.401
2.867SerPro: 2.867 ± 0.457
3.497SerGln: 3.497 ± 0.434
3.555SerArg: 3.555 ± 0.434
4.529SerSer: 4.529 ± 0.571
3.899SerThr: 3.899 ± 0.553
4.759SerVal: 4.759 ± 0.61
0.803SerTrp: 0.803 ± 0.225
1.835SerTyr: 1.835 ± 0.384
0.0SerXaa: 0.0 ± 0.0
Thr
5.619ThrAla: 5.619 ± 0.629
0.516ThrCys: 0.516 ± 0.153
2.752ThrAsp: 2.752 ± 0.389
3.841ThrGlu: 3.841 ± 0.724
2.351ThrPhe: 2.351 ± 0.363
5.905ThrGly: 5.905 ± 0.717
1.319ThrHis: 1.319 ± 0.273
3.268ThrIle: 3.268 ± 0.42
2.465ThrLys: 2.465 ± 0.352
5.561ThrLeu: 5.561 ± 0.582
0.573ThrMet: 0.573 ± 0.16
1.835ThrAsn: 1.835 ± 0.303
3.555ThrPro: 3.555 ± 0.54
2.121ThrGln: 2.121 ± 0.285
2.637ThrArg: 2.637 ± 0.376
3.784ThrSer: 3.784 ± 0.638
3.956ThrThr: 3.956 ± 0.454
5.16ThrVal: 5.16 ± 0.607
0.803ThrTrp: 0.803 ± 0.255
1.491ThrTyr: 1.491 ± 0.34
0.0ThrXaa: 0.0 ± 0.0
Val
6.421ValAla: 6.421 ± 0.646
0.86ValCys: 0.86 ± 0.217
3.096ValAsp: 3.096 ± 0.373
3.956ValGlu: 3.956 ± 0.491
2.236ValPhe: 2.236 ± 0.325
3.899ValGly: 3.899 ± 0.483
0.745ValHis: 0.745 ± 0.207
3.268ValIle: 3.268 ± 0.414
3.383ValLys: 3.383 ± 0.443
5.791ValLeu: 5.791 ± 0.574
1.835ValMet: 1.835 ± 0.272
3.44ValAsn: 3.44 ± 0.371
3.325ValPro: 3.325 ± 0.478
2.064ValGln: 2.064 ± 0.444
4.931ValArg: 4.931 ± 0.551
5.16ValSer: 5.16 ± 0.727
4.701ValThr: 4.701 ± 0.614
4.701ValVal: 4.701 ± 0.488
1.089ValTrp: 1.089 ± 0.265
2.121ValTyr: 2.121 ± 0.365
0.0ValXaa: 0.0 ± 0.0
Trp
1.204TrpAla: 1.204 ± 0.239
0.516TrpCys: 0.516 ± 0.175
0.688TrpAsp: 0.688 ± 0.237
0.459TrpGlu: 0.459 ± 0.132
0.745TrpPhe: 0.745 ± 0.208
0.803TrpGly: 0.803 ± 0.19
0.516TrpHis: 0.516 ± 0.16
0.745TrpIle: 0.745 ± 0.228
1.089TrpLys: 1.089 ± 0.238
1.949TrpLeu: 1.949 ± 0.424
0.516TrpMet: 0.516 ± 0.156
0.459TrpAsn: 0.459 ± 0.161
0.516TrpPro: 0.516 ± 0.14
1.089TrpGln: 1.089 ± 0.227
1.663TrpArg: 1.663 ± 0.258
0.975TrpSer: 0.975 ± 0.191
0.745TrpThr: 0.745 ± 0.153
1.777TrpVal: 1.777 ± 0.304
0.516TrpTrp: 0.516 ± 0.249
0.459TrpTyr: 0.459 ± 0.144
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.695TyrAla: 2.695 ± 0.336
0.229TyrCys: 0.229 ± 0.116
1.892TyrAsp: 1.892 ± 0.399
0.917TyrGlu: 0.917 ± 0.243
1.376TyrPhe: 1.376 ± 0.297
2.064TyrGly: 2.064 ± 0.351
0.631TyrHis: 0.631 ± 0.176
1.376TyrIle: 1.376 ± 0.268
1.147TyrLys: 1.147 ± 0.291
1.663TyrLeu: 1.663 ± 0.279
0.573TyrMet: 0.573 ± 0.183
0.803TyrAsn: 0.803 ± 0.209
1.376TyrPro: 1.376 ± 0.28
1.491TyrGln: 1.491 ± 0.382
2.179TyrArg: 2.179 ± 0.383
1.72TyrSer: 1.72 ± 0.309
1.605TyrThr: 1.605 ± 0.235
2.007TyrVal: 2.007 ± 0.437
0.401TyrTrp: 0.401 ± 0.129
0.745TyrTyr: 0.745 ± 0.241
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 85 proteins (17443 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski