Desulfurobacterium thermolithotrophum (strain DSM 11699 / BSA)

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Desulfurobacteriales; Desulfurobacteriaceae; Desulfurobacterium; Desulfurobacterium thermolithotrophum

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1496 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0S1R5|F0S1R5_DESTD RNA methyltransferase TrmH family group 3 OS=Desulfurobacterium thermolithotrophum (strain DSM 11699 / BSA) OX=868864 GN=Dester_0263 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 4.96EE3 pKa = 3.88KK4 pKa = 10.31QYY6 pKa = 11.56KK7 pKa = 9.53LWRR10 pKa = 11.84CTVCGYY16 pKa = 10.32IYY18 pKa = 10.68DD19 pKa = 3.92VDD21 pKa = 5.16EE22 pKa = 5.28GDD24 pKa = 4.2PDD26 pKa = 4.24NNVPPGTSFEE36 pKa = 5.24AIPDD40 pKa = 3.51DD41 pKa = 4.45WVCPICGVTKK51 pKa = 10.7DD52 pKa = 3.49KK53 pKa = 11.29FEE55 pKa = 4.57PVDD58 pKa = 3.63DD59 pKa = 4.2

Molecular weight: 6.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0S3F4|F0S3F4_DESTD 6-carboxyhexanoate--CoA ligase OS=Desulfurobacterium thermolithotrophum (strain DSM 11699 / BSA) OX=868864 GN=Dester_0730 PE=4 SV=1
MM1 pKa = 7.29EE2 pKa = 5.72VIPKK6 pKa = 10.3RR7 pKa = 11.84KK8 pKa = 9.05FKK10 pKa = 11.13CFDD13 pKa = 3.69CGHH16 pKa = 6.17EE17 pKa = 4.13FEE19 pKa = 5.34EE20 pKa = 5.14PFGKK24 pKa = 9.19PRR26 pKa = 11.84WMVKK30 pKa = 10.07CPKK33 pKa = 9.79CGSEE37 pKa = 4.0NIVRR41 pKa = 11.84ADD43 pKa = 4.54TPAGWCGKK51 pKa = 10.25GYY53 pKa = 10.79GRR55 pKa = 11.84GAGRR59 pKa = 11.84GRR61 pKa = 11.84GRR63 pKa = 11.84GWGWFGGWRR72 pKa = 11.84RR73 pKa = 11.84GEE75 pKa = 3.79GRR77 pKa = 11.84GRR79 pKa = 11.84FGFGWGRR86 pKa = 11.84KK87 pKa = 5.06WW88 pKa = 3.14

Molecular weight: 10.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1496

0

1496

473549

30

1470

316.5

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.947 ± 0.063

0.964 ± 0.033

4.499 ± 0.045

8.602 ± 0.085

5.163 ± 0.059

6.578 ± 0.058

1.474 ± 0.027

8.507 ± 0.053

10.095 ± 0.086

10.08 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.816 ± 0.026

4.026 ± 0.052

3.826 ± 0.041

2.067 ± 0.028

4.517 ± 0.051

5.605 ± 0.05

4.587 ± 0.047

7.241 ± 0.051

0.794 ± 0.018

3.614 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski