Amino acid dipepetide frequency for Desulfurobacterium thermolithotrophum (strain DSM 11699 / BSA)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.152AlaAla: 4.152 ± 0.134	0.602AlaCys: 0.602 ± 0.043	2.749AlaAsp: 2.749 ± 0.088	4.445AlaGlu: 4.445 ± 0.096	3.195AlaPhe: 3.195 ± 0.095	4.487AlaGly: 4.487 ± 0.119	0.94AlaHis: 0.94 ± 0.046	5.372AlaIle: 5.372 ± 0.116	5.607AlaLys: 5.607 ± 0.123	6.36AlaLeu: 6.36 ± 0.125	1.115AlaMet: 1.115 ± 0.052	1.939AlaAsn: 1.939 ± 0.076	1.744AlaPro: 1.744 ± 0.072	1.292AlaGln: 1.292 ± 0.058	2.492AlaArg: 2.492 ± 0.073	2.754AlaSer: 2.754 ± 0.071	2.707AlaThr: 2.707 ± 0.081	4.998AlaVal: 4.998 ± 0.106	0.382AlaTrp: 0.382 ± 0.03	2.133AlaTyr: 2.133 ± 0.068	0.0AlaXaa: 0.0 ± 0.0
Cys	0.494CysAla: 0.494 ± 0.033	0.131CysCys: 0.131 ± 0.021	0.399CysAsp: 0.399 ± 0.028	0.634CysGlu: 0.634 ± 0.036	0.397CysPhe: 0.397 ± 0.033	1.037CysGly: 1.037 ± 0.054	0.376CysHis: 0.376 ± 0.078	0.638CysIle: 0.638 ± 0.044	0.902CysLys: 0.902 ± 0.049	0.691CysLeu: 0.691 ± 0.042	0.184CysMet: 0.184 ± 0.018	0.403CysAsn: 0.403 ± 0.029	0.606CysPro: 0.606 ± 0.046	0.258CysGln: 0.258 ± 0.027	0.473CysArg: 0.473 ± 0.031	0.638CysSer: 0.638 ± 0.042	0.386CysThr: 0.386 ± 0.03	0.576CysVal: 0.576 ± 0.033	0.057CysTrp: 0.057 ± 0.01	0.357CysTyr: 0.357 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	2.405AspAla: 2.405 ± 0.081	0.427AspCys: 0.427 ± 0.031	1.565AspAsp: 1.565 ± 0.065	3.653AspGlu: 3.653 ± 0.098	2.971AspPhe: 2.971 ± 0.084	2.726AspGly: 2.726 ± 0.093	0.532AspHis: 0.532 ± 0.035	4.443AspIle: 4.443 ± 0.116	3.949AspLys: 3.949 ± 0.107	4.682AspLeu: 4.682 ± 0.106	0.91AspMet: 0.91 ± 0.043	1.537AspAsn: 1.537 ± 0.068	1.835AspPro: 1.835 ± 0.08	0.625AspGln: 0.625 ± 0.038	2.276AspArg: 2.276 ± 0.075	2.361AspSer: 2.361 ± 0.073	1.926AspThr: 1.926 ± 0.064	3.607AspVal: 3.607 ± 0.095	0.496AspTrp: 0.496 ± 0.036	2.063AspTyr: 2.063 ± 0.072	0.0AspXaa: 0.0 ± 0.0
Glu	4.829GluAla: 4.829 ± 0.116	0.513GluCys: 0.513 ± 0.038	3.535GluAsp: 3.535 ± 0.098	9.019GluGlu: 9.019 ± 0.201	3.617GluPhe: 3.617 ± 0.091	4.834GluGly: 4.834 ± 0.103	1.003GluHis: 1.003 ± 0.052	8.681GluIle: 8.681 ± 0.161	11.131GluLys: 11.131 ± 0.218	8.673GluLeu: 8.673 ± 0.138	1.626GluMet: 1.626 ± 0.059	4.12GluAsn: 4.12 ± 0.099	2.116GluPro: 2.116 ± 0.061	1.398GluGln: 1.398 ± 0.06	4.373GluArg: 4.373 ± 0.109	3.556GluSer: 3.556 ± 0.086	3.636GluThr: 3.636 ± 0.085	6.432GluVal: 6.432 ± 0.127	0.511GluTrp: 0.511 ± 0.035	2.414GluTyr: 2.414 ± 0.066	0.0GluXaa: 0.0 ± 0.0
Phe	2.536PheAla: 2.536 ± 0.079	0.511PheCys: 0.511 ± 0.028	2.361PheAsp: 2.361 ± 0.075	3.951PheGlu: 3.951 ± 0.095	3.032PhePhe: 3.032 ± 0.113	3.486PheGly: 3.486 ± 0.096	0.904PheHis: 0.904 ± 0.048	4.382PheIle: 4.382 ± 0.109	4.601PheLys: 4.601 ± 0.099	5.866PheLeu: 5.866 ± 0.142	0.974PheMet: 0.974 ± 0.041	1.958PheAsn: 1.958 ± 0.068	2.042PhePro: 2.042 ± 0.061	1.128PheGln: 1.128 ± 0.048	1.926PheArg: 1.926 ± 0.068	3.888PheSer: 3.888 ± 0.106	2.26PheThr: 2.26 ± 0.081	3.455PheVal: 3.455 ± 0.092	0.386PheTrp: 0.386 ± 0.031	1.979PheTyr: 1.979 ± 0.078	0.0PheXaa: 0.0 ± 0.0
Gly	4.095GlyAla: 4.095 ± 0.127	0.779GlyCys: 0.779 ± 0.056	2.861GlyAsp: 2.861 ± 0.084	4.849GlyGlu: 4.849 ± 0.1	3.533GlyPhe: 3.533 ± 0.089	4.356GlyGly: 4.356 ± 0.131	1.117GlyHis: 1.117 ± 0.048	6.981GlyIle: 6.981 ± 0.132	7.243GlyLys: 7.243 ± 0.148	5.619GlyLeu: 5.619 ± 0.121	1.51GlyMet: 1.51 ± 0.064	2.804GlyAsn: 2.804 ± 0.1	1.263GlyPro: 1.263 ± 0.049	1.145GlyGln: 1.145 ± 0.042	3.138GlyArg: 3.138 ± 0.081	3.182GlySer: 3.182 ± 0.087	3.455GlyThr: 3.455 ± 0.096	4.633GlyVal: 4.633 ± 0.108	0.6GlyTrp: 0.6 ± 0.039	2.614GlyTyr: 2.614 ± 0.079	0.0GlyXaa: 0.0 ± 0.0
His	0.809HisAla: 0.809 ± 0.044	0.218HisCys: 0.218 ± 0.02	0.526HisAsp: 0.526 ± 0.036	0.783HisGlu: 0.783 ± 0.047	0.809HisPhe: 0.809 ± 0.042	1.043HisGly: 1.043 ± 0.057	0.403HisHis: 0.403 ± 0.033	1.383HisIle: 1.383 ± 0.056	1.153HisLys: 1.153 ± 0.057	1.495HisLeu: 1.495 ± 0.057	0.277HisMet: 0.277 ± 0.025	0.536HisAsn: 0.536 ± 0.04	0.9HisPro: 0.9 ± 0.05	0.424HisGln: 0.424 ± 0.035	0.684HisArg: 0.684 ± 0.038	0.893HisSer: 0.893 ± 0.045	0.648HisThr: 0.648 ± 0.038	0.984HisVal: 0.984 ± 0.052	0.139HisTrp: 0.139 ± 0.017	0.634HisTyr: 0.634 ± 0.037	0.0HisXaa: 0.0 ± 0.0
Ile	5.972IleAla: 5.972 ± 0.103	0.79IleCys: 0.79 ± 0.051	4.654IleAsp: 4.654 ± 0.105	7.917IleGlu: 7.917 ± 0.143	3.907IlePhe: 3.907 ± 0.107	5.37IleGly: 5.37 ± 0.132	1.271IleHis: 1.271 ± 0.055	5.581IleIle: 5.581 ± 0.129	7.807IleLys: 7.807 ± 0.14	8.369IleLeu: 8.369 ± 0.162	1.218IleMet: 1.218 ± 0.057	3.271IleAsn: 3.271 ± 0.093	4.213IlePro: 4.213 ± 0.093	1.567IleGln: 1.567 ± 0.052	3.455IleArg: 3.455 ± 0.092	5.592IleSer: 5.592 ± 0.104	4.035IleThr: 4.035 ± 0.108	6.544IleVal: 6.544 ± 0.133	0.596IleTrp: 0.596 ± 0.034	2.944IleTyr: 2.944 ± 0.077	0.0IleXaa: 0.0 ± 0.0
Lys	5.881LysAla: 5.881 ± 0.115	0.794LysCys: 0.794 ± 0.042	4.747LysAsp: 4.747 ± 0.101	11.878LysGlu: 11.878 ± 0.2	4.141LysPhe: 4.141 ± 0.102	6.576LysGly: 6.576 ± 0.121	1.164LysHis: 1.164 ± 0.049	8.058LysIle: 8.058 ± 0.119	10.402LysLys: 10.402 ± 0.17	10.212LysLeu: 10.212 ± 0.186	1.537LysMet: 1.537 ± 0.067	4.407LysAsn: 4.407 ± 0.108	3.324LysPro: 3.324 ± 0.083	1.907LysGln: 1.907 ± 0.07	5.201LysArg: 5.201 ± 0.133	4.53LysSer: 4.53 ± 0.102	4.452LysThr: 4.452 ± 0.097	7.883LysVal: 7.883 ± 0.166	0.737LysTrp: 0.737 ± 0.039	3.115LysTyr: 3.115 ± 0.087	0.0LysXaa: 0.0 ± 0.0
Leu	5.645LeuAla: 5.645 ± 0.13	0.87LeuCys: 0.87 ± 0.05	4.318LeuAsp: 4.318 ± 0.118	8.084LeuGlu: 8.084 ± 0.152	4.929LeuPhe: 4.929 ± 0.125	6.24LeuGly: 6.24 ± 0.113	1.318LeuHis: 1.318 ± 0.049	7.908LeuIle: 7.908 ± 0.163	12.14LeuLys: 12.14 ± 0.204	9.953LeuLeu: 9.953 ± 0.201	1.795LeuMet: 1.795 ± 0.062	4.253LeuAsn: 4.253 ± 0.109	4.34LeuPro: 4.34 ± 0.096	2.403LeuGln: 2.403 ± 0.088	4.779LeuArg: 4.779 ± 0.1	7.404LeuSer: 7.404 ± 0.163	4.794LeuThr: 4.794 ± 0.103	5.609LeuVal: 5.609 ± 0.121	0.786LeuTrp: 0.786 ± 0.046	3.233LeuTyr: 3.233 ± 0.089	0.0LeuXaa: 0.0 ± 0.0
Met	1.411MetAla: 1.411 ± 0.055	0.199MetCys: 0.199 ± 0.023	0.853MetAsp: 0.853 ± 0.045	1.698MetGlu: 1.698 ± 0.058	0.754MetPhe: 0.754 ± 0.04	1.343MetGly: 1.343 ± 0.051	0.213MetHis: 0.213 ± 0.019	1.307MetIle: 1.307 ± 0.048	2.3MetLys: 2.3 ± 0.061	1.558MetLeu: 1.558 ± 0.053	0.348MetMet: 0.348 ± 0.031	0.781MetAsn: 0.781 ± 0.039	0.718MetPro: 0.718 ± 0.033	0.304MetGln: 0.304 ± 0.025	0.954MetArg: 0.954 ± 0.049	0.851MetSer: 0.851 ± 0.038	0.809MetThr: 0.809 ± 0.043	1.202MetVal: 1.202 ± 0.056	0.127MetTrp: 0.127 ± 0.016	0.431MetTyr: 0.431 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.26AsnAla: 2.26 ± 0.069	0.439AsnCys: 0.439 ± 0.032	1.423AsnAsp: 1.423 ± 0.062	2.405AsnGlu: 2.405 ± 0.072	2.593AsnPhe: 2.593 ± 0.085	2.54AsnGly: 2.54 ± 0.085	0.517AsnHis: 0.517 ± 0.035	3.48AsnIle: 3.48 ± 0.092	3.136AsnLys: 3.136 ± 0.085	5.022AsnLeu: 5.022 ± 0.118	0.663AsnMet: 0.663 ± 0.034	1.324AsnAsn: 1.324 ± 0.065	2.304AsnPro: 2.304 ± 0.072	0.874AsnGln: 0.874 ± 0.047	1.934AsnArg: 1.934 ± 0.074	2.306AsnSer: 2.306 ± 0.082	1.698AsnThr: 1.698 ± 0.068	2.954AsnVal: 2.954 ± 0.087	0.462AsnTrp: 0.462 ± 0.031	1.924AsnTyr: 1.924 ± 0.065	0.0AsnXaa: 0.0 ± 0.0
Pro	2.017ProAla: 2.017 ± 0.069	0.363ProCys: 0.363 ± 0.03	2.021ProAsp: 2.021 ± 0.073	4.082ProGlu: 4.082 ± 0.095	2.31ProPhe: 2.31 ± 0.073	2.464ProGly: 2.464 ± 0.074	0.741ProHis: 0.741 ± 0.042	3.041ProIle: 3.041 ± 0.098	3.075ProLys: 3.075 ± 0.094	3.427ProLeu: 3.427 ± 0.072	0.581ProMet: 0.581 ± 0.035	1.415ProAsn: 1.415 ± 0.057	1.347ProPro: 1.347 ± 0.066	1.09ProGln: 1.09 ± 0.051	1.197ProArg: 1.197 ± 0.051	2.169ProSer: 2.169 ± 0.072	1.888ProThr: 1.888 ± 0.069	3.168ProVal: 3.168 ± 0.081	0.277ProTrp: 0.277 ± 0.024	1.592ProTyr: 1.592 ± 0.049	0.0ProXaa: 0.0 ± 0.0
Gln	1.183GlnAla: 1.183 ± 0.061	0.207GlnCys: 0.207 ± 0.022	0.872GlnAsp: 0.872 ± 0.033	1.751GlnGlu: 1.751 ± 0.062	1.064GlnPhe: 1.064 ± 0.052	1.107GlnGly: 1.107 ± 0.049	0.266GlnHis: 0.266 ± 0.026	1.966GlnIle: 1.966 ± 0.07	2.276GlnLys: 2.276 ± 0.075	2.146GlnLeu: 2.146 ± 0.078	0.467GlnMet: 0.467 ± 0.032	0.847GlnAsn: 0.847 ± 0.041	0.691GlnPro: 0.691 ± 0.037	0.519GlnGln: 0.519 ± 0.033	0.974GlnArg: 0.974 ± 0.051	0.976GlnSer: 0.976 ± 0.045	1.085GlnThr: 1.085 ± 0.054	1.516GlnVal: 1.516 ± 0.063	0.16GlnTrp: 0.16 ± 0.02	0.593GlnTyr: 0.593 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.804ArgAla: 2.804 ± 0.078	0.433ArgCys: 0.433 ± 0.032	1.803ArgAsp: 1.803 ± 0.061	4.361ArgGlu: 4.361 ± 0.096	2.572ArgPhe: 2.572 ± 0.083	2.749ArgGly: 2.749 ± 0.093	0.604ArgHis: 0.604 ± 0.039	4.04ArgIle: 4.04 ± 0.106	5.241ArgLys: 5.241 ± 0.114	4.255ArgLeu: 4.255 ± 0.103	0.809ArgMet: 0.809 ± 0.042	1.989ArgAsn: 1.989 ± 0.062	1.476ArgPro: 1.476 ± 0.058	0.935ArgGln: 0.935 ± 0.045	2.374ArgArg: 2.374 ± 0.087	1.846ArgSer: 1.846 ± 0.067	1.814ArgThr: 1.814 ± 0.066	3.077ArgVal: 3.077 ± 0.081	0.424ArgTrp: 0.424 ± 0.031	1.558ArgTyr: 1.558 ± 0.06	0.0ArgXaa: 0.0 ± 0.0
Ser	2.971SerAla: 2.971 ± 0.079	0.617SerCys: 0.617 ± 0.035	2.287SerAsp: 2.287 ± 0.079	4.042SerGlu: 4.042 ± 0.096	3.624SerPhe: 3.624 ± 0.114	3.902SerGly: 3.902 ± 0.101	0.84SerHis: 0.84 ± 0.043	4.882SerIle: 4.882 ± 0.118	4.87SerLys: 4.87 ± 0.111	6.339SerLeu: 6.339 ± 0.136	1.096SerMet: 1.096 ± 0.049	2.135SerAsn: 2.135 ± 0.067	2.274SerPro: 2.274 ± 0.084	1.404SerGln: 1.404 ± 0.047	2.072SerArg: 2.072 ± 0.061	3.575SerSer: 3.575 ± 0.094	2.798SerThr: 2.798 ± 0.082	3.522SerVal: 3.522 ± 0.087	0.475SerTrp: 0.475 ± 0.034	2.325SerTyr: 2.325 ± 0.089	0.0SerXaa: 0.0 ± 0.0
Thr	3.161ThrAla: 3.161 ± 0.087	0.524ThrCys: 0.524 ± 0.047	2.146ThrAsp: 2.146 ± 0.064	3.168ThrGlu: 3.168 ± 0.074	2.492ThrPhe: 2.492 ± 0.069	3.845ThrGly: 3.845 ± 0.101	0.699ThrHis: 0.699 ± 0.039	3.592ThrIle: 3.592 ± 0.094	3.375ThrLys: 3.375 ± 0.08	4.817ThrLeu: 4.817 ± 0.115	0.779ThrMet: 0.779 ± 0.043	1.689ThrAsn: 1.689 ± 0.075	2.268ThrPro: 2.268 ± 0.07	0.868ThrGln: 0.868 ± 0.043	1.611ThrArg: 1.611 ± 0.062	2.543ThrSer: 2.543 ± 0.076	2.325ThrThr: 2.325 ± 0.083	3.833ThrVal: 3.833 ± 0.088	0.355ThrTrp: 0.355 ± 0.032	1.778ThrTyr: 1.778 ± 0.064	0.0ThrXaa: 0.0 ± 0.0
Val	4.705ValAla: 4.705 ± 0.103	0.712ValCys: 0.712 ± 0.043	3.748ValAsp: 3.748 ± 0.101	6.28ValGlu: 6.28 ± 0.126	3.29ValPhe: 3.29 ± 0.083	4.699ValGly: 4.699 ± 0.115	1.033ValHis: 1.033 ± 0.048	5.909ValIle: 5.909 ± 0.114	7.579ValLys: 7.579 ± 0.148	6.728ValLeu: 6.728 ± 0.129	1.335ValMet: 1.335 ± 0.059	2.969ValAsn: 2.969 ± 0.069	2.768ValPro: 2.768 ± 0.076	1.307ValGln: 1.307 ± 0.048	3.02ValArg: 3.02 ± 0.077	4.337ValSer: 4.337 ± 0.098	3.25ValThr: 3.25 ± 0.086	5.729ValVal: 5.729 ± 0.124	0.53ValTrp: 0.53 ± 0.035	2.479ValTyr: 2.479 ± 0.072	0.0ValXaa: 0.0 ± 0.0
Trp	0.403TrpAla: 0.403 ± 0.031	0.108TrpCys: 0.108 ± 0.015	0.424TrpAsp: 0.424 ± 0.032	0.606TrpGlu: 0.606 ± 0.034	0.37TrpPhe: 0.37 ± 0.029	0.496TrpGly: 0.496 ± 0.033	0.148TrpHis: 0.148 ± 0.018	0.819TrpIle: 0.819 ± 0.045	0.904TrpLys: 0.904 ± 0.045	0.716TrpLeu: 0.716 ± 0.041	0.27TrpMet: 0.27 ± 0.026	0.488TrpAsn: 0.488 ± 0.027	0.169TrpPro: 0.169 ± 0.019	0.203TrpGln: 0.203 ± 0.019	0.399TrpArg: 0.399 ± 0.028	0.355TrpSer: 0.355 ± 0.025	0.279TrpThr: 0.279 ± 0.024	0.401TrpVal: 0.401 ± 0.032	0.087TrpTrp: 0.087 ± 0.016	0.298TrpTyr: 0.298 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.734TyrAla: 1.734 ± 0.061	0.401TyrCys: 0.401 ± 0.032	1.696TyrAsp: 1.696 ± 0.056	2.414TyrGlu: 2.414 ± 0.074	2.025TyrPhe: 2.025 ± 0.086	2.475TyrGly: 2.475 ± 0.069	0.653TyrHis: 0.653 ± 0.035	2.608TyrIle: 2.608 ± 0.082	3.258TyrLys: 3.258 ± 0.098	3.871TyrLeu: 3.871 ± 0.099	0.665TyrMet: 0.665 ± 0.038	1.394TyrAsn: 1.394 ± 0.059	1.867TyrPro: 1.867 ± 0.066	1.014TyrGln: 1.014 ± 0.052	1.833TyrArg: 1.833 ± 0.067	2.298TyrSer: 2.298 ± 0.071	1.622TyrThr: 1.622 ± 0.06	2.283TyrVal: 2.283 ± 0.075	0.355TyrTrp: 0.355 ± 0.028	1.681TyrTyr: 1.681 ± 0.064	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1496 proteins (473549 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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