Desulfosporosinus sp. OL
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5425 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q8QVW0|A0A1Q8QVW0_9FIRM Nucleotide-binding protein OS=Desulfosporosinus sp. OL OX=1888891 GN=DSOL_2611 PE=4 SV=1
MM1 pKa = 8.44 DD2 pKa = 3.86 YY3 pKa = 9.99 PYY5 pKa = 10.81 LIGYY9 pKa = 7.99 VYY11 pKa = 10.54 SEE13 pKa = 4.48 EE14 pKa = 4.14 GRR16 pKa = 11.84 SEE18 pKa = 4.67 HH19 pKa = 6.3 FLKK22 pKa = 8.3 TTPTNIASFIIKK34 pKa = 10.21 NSPLDD39 pKa = 3.78 VIQITTPLDD48 pKa = 3.26 TAFISTRR55 pKa = 11.84 AGFIDD60 pKa = 3.8 YY61 pKa = 10.83 CADD64 pKa = 3.4 QEE66 pKa = 4.28 FLRR69 pKa = 11.84 NEE71 pKa = 4.32 LLPVLIPMQMGDD83 pKa = 3.74 TEE85 pKa = 4.22 PSEE88 pKa = 4.35 VEE90 pKa = 3.81 LVPEE94 pKa = 4.03 NEE96 pKa = 4.21 MNSMDD101 pKa = 4.65 EE102 pKa = 4.3 EE103 pKa = 4.96 GLDD106 pKa = 3.52 SLEE109 pKa = 4.13 FF110 pKa = 4.75
Molecular weight: 12.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.872
IPC_protein 3.783
Toseland 3.605
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.617
Grimsley 3.528
Solomon 3.732
Lehninger 3.694
Nozaki 3.884
DTASelect 4.012
Thurlkill 3.656
EMBOSS 3.668
Sillero 3.897
Patrickios 1.85
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A1Q8QM20|A0A1Q8QM20_9FIRM MacB_PCD domain-containing protein OS=Desulfosporosinus sp. OL OX=1888891 GN=DSOL_4043 PE=4 SV=1
MM1 pKa = 7.19 RR2 pKa = 11.84 QRR4 pKa = 11.84 VRR6 pKa = 11.84 MGIMGTAKK14 pKa = 10.29 GPHH17 pKa = 5.49 IQALATALQNRR28 pKa = 11.84 GAEE31 pKa = 4.14 VSFVSPQRR39 pKa = 11.84 LTSFIPEE46 pKa = 4.08 PATIITGAGGSRR58 pKa = 11.84 EE59 pKa = 3.96 NLRR62 pKa = 11.84 DD63 pKa = 3.48 LDD65 pKa = 4.05 ALLVRR70 pKa = 11.84 SLPGGSLEE78 pKa = 4.01 QVIYY82 pKa = 10.48 RR83 pKa = 11.84 VDD85 pKa = 3.25 VLHH88 pKa = 6.95 RR89 pKa = 11.84 LEE91 pKa = 4.36 KK92 pKa = 10.88 LGLKK96 pKa = 9.62 VINRR100 pKa = 11.84 AAVLEE105 pKa = 4.3 KK106 pKa = 10.71 NSRR109 pKa = 11.84 QVLYY113 pKa = 9.27 VCFVGRR119 pKa = 11.84 CGLASTQNGRR129 pKa = 11.84 DD130 pKa = 3.16 RR131 pKa = 11.84 KK132 pKa = 10.18 LRR134 pKa = 11.84 SGDD137 pKa = 3.64 GSCEE141 pKa = 3.72 EE142 pKa = 5.11 DD143 pKa = 3.4 GGSGCEE149 pKa = 3.77 TSFWLPRR156 pKa = 11.84 NRR158 pKa = 11.84 HH159 pKa = 4.13 GLGRR163 pKa = 11.84 GSRR166 pKa = 11.84 GGLPRR171 pKa = 11.84 FPSPGIGPVCVLFTRR186 pKa = 11.84 ISTAA190 pKa = 2.91
Molecular weight: 20.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.443
IPC_protein 10.394
Toseland 10.687
ProMoST 10.496
Dawson 10.76
Bjellqvist 10.54
Wikipedia 11.008
Rodwell 10.789
Grimsley 10.789
Solomon 10.95
Lehninger 10.906
Nozaki 10.716
DTASelect 10.511
Thurlkill 10.687
EMBOSS 11.111
Sillero 10.716
Patrickios 10.54
IPC_peptide 10.965
IPC2_peptide 9.94
IPC2.peptide.svr19 8.678
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5425
0
5425
1464743
37
2863
270.0
30.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.619 ± 0.037
1.12 ± 0.014
4.915 ± 0.029
6.904 ± 0.044
4.033 ± 0.029
7.473 ± 0.035
1.923 ± 0.017
7.151 ± 0.029
6.08 ± 0.032
10.419 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.719 ± 0.019
4.035 ± 0.023
3.951 ± 0.022
3.842 ± 0.025
4.838 ± 0.03
6.025 ± 0.027
5.402 ± 0.033
7.288 ± 0.034
1.086 ± 0.013
3.178 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here