Amino acid dipepetide frequency for NL63-related bat coronavirus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.509AlaAla: 5.509 ± 0.617
1.811AlaCys: 1.811 ± 0.36
2.038AlaAsp: 2.038 ± 0.594
3.019AlaGlu: 3.019 ± 0.516
5.207AlaPhe: 5.207 ± 0.909
4.302AlaGly: 4.302 ± 1.01
1.358AlaHis: 1.358 ± 0.268
4.151AlaIle: 4.151 ± 1.582
3.547AlaLys: 3.547 ± 0.93
5.132AlaLeu: 5.132 ± 1.072
1.962AlaMet: 1.962 ± 0.426
3.094AlaAsn: 3.094 ± 0.312
2.49AlaPro: 2.49 ± 0.277
2.038AlaGln: 2.038 ± 0.333
2.566AlaArg: 2.566 ± 0.762
5.811AlaSer: 5.811 ± 1.187
4.075AlaThr: 4.075 ± 0.304
5.735AlaVal: 5.735 ± 0.715
0.83AlaTrp: 0.83 ± 0.21
3.396AlaTyr: 3.396 ± 0.759
0.0AlaXaa: 0.0 ± 0.0
Cys
2.641CysAla: 2.641 ± 0.574
0.906CysCys: 0.906 ± 0.325
2.264CysAsp: 2.264 ± 0.389
0.981CysGlu: 0.981 ± 0.261
2.415CysPhe: 2.415 ± 0.388
2.641CysGly: 2.641 ± 0.574
0.075CysHis: 0.075 ± 0.231
1.358CysIle: 1.358 ± 0.348
1.509CysLys: 1.509 ± 0.325
1.736CysLeu: 1.736 ± 0.293
0.453CysMet: 0.453 ± 0.174
2.264CysAsn: 2.264 ± 0.549
0.83CysPro: 0.83 ± 0.191
0.302CysGln: 0.302 ± 0.26
1.358CysArg: 1.358 ± 0.481
1.736CysSer: 1.736 ± 0.392
2.038CysThr: 2.038 ± 0.494
3.019CysVal: 3.019 ± 0.638
0.83CysTrp: 0.83 ± 0.414
2.566CysTyr: 2.566 ± 0.619
0.0CysXaa: 0.0 ± 0.0
Asp
2.792AspAla: 2.792 ± 0.349
1.283AspCys: 1.283 ± 0.287
2.943AspAsp: 2.943 ± 0.413
1.509AspGlu: 1.509 ± 0.308
4.679AspPhe: 4.679 ± 0.388
5.66AspGly: 5.66 ± 1.037
1.057AspHis: 1.057 ± 0.233
3.471AspIle: 3.471 ± 0.386
3.321AspLys: 3.321 ± 0.555
3.924AspLeu: 3.924 ± 0.326
0.981AspMet: 0.981 ± 0.368
3.773AspAsn: 3.773 ± 0.383
2.038AspPro: 2.038 ± 0.306
0.528AspGln: 0.528 ± 0.512
1.887AspArg: 1.887 ± 0.268
3.547AspSer: 3.547 ± 0.616
1.283AspThr: 1.283 ± 0.25
7.32AspVal: 7.32 ± 1.323
0.83AspTrp: 0.83 ± 0.321
3.622AspTyr: 3.622 ± 0.619
0.0AspXaa: 0.0 ± 0.0
Glu
2.264GluAla: 2.264 ± 0.425
0.981GluCys: 0.981 ± 0.231
2.038GluAsp: 2.038 ± 0.357
2.415GluGlu: 2.415 ± 0.301
2.415GluPhe: 2.415 ± 0.347
3.698GluGly: 3.698 ± 0.695
1.509GluHis: 1.509 ± 0.418
1.509GluIle: 1.509 ± 0.219
1.585GluLys: 1.585 ± 0.47
3.849GluLeu: 3.849 ± 0.534
0.528GluMet: 0.528 ± 0.139
2.717GluAsn: 2.717 ± 0.143
1.736GluPro: 1.736 ± 0.379
1.509GluGln: 1.509 ± 0.219
1.434GluArg: 1.434 ± 0.24
2.339GluSer: 2.339 ± 0.423
1.736GluThr: 1.736 ± 0.29
2.868GluVal: 2.868 ± 0.399
0.679GluTrp: 0.679 ± 0.163
0.981GluTyr: 0.981 ± 0.3
0.151GluXaa: 0.151 ± 0.057
Phe
3.924PheAla: 3.924 ± 0.788
2.113PheCys: 2.113 ± 0.288
5.283PheAsp: 5.283 ± 0.62
1.509PheGlu: 1.509 ± 0.228
2.415PhePhe: 2.415 ± 0.704
5.132PheGly: 5.132 ± 0.461
0.679PheHis: 0.679 ± 0.268
3.17PheIle: 3.17 ± 0.557
3.924PheLys: 3.924 ± 0.805
4.905PheLeu: 4.905 ± 1.101
1.358PheMet: 1.358 ± 0.245
3.698PheAsn: 3.698 ± 0.757
0.83PhePro: 0.83 ± 0.261
0.83PheGln: 0.83 ± 0.676
1.585PheArg: 1.585 ± 0.267
4.075PheSer: 4.075 ± 0.817
3.094PheThr: 3.094 ± 0.382
7.018PheVal: 7.018 ± 0.95
1.057PheTrp: 1.057 ± 0.242
2.264PheTyr: 2.264 ± 0.407
0.0PheXaa: 0.0 ± 0.0
Gly
3.698GlyAla: 3.698 ± 0.386
3.547GlyCys: 3.547 ± 0.783
5.358GlyAsp: 5.358 ± 0.715
1.283GlyGlu: 1.283 ± 0.321
4.075GlyPhe: 4.075 ± 0.587
4.151GlyGly: 4.151 ± 0.478
1.207GlyHis: 1.207 ± 0.979
1.962GlyIle: 1.962 ± 0.317
4.0GlyLys: 4.0 ± 0.397
5.811GlyLeu: 5.811 ± 0.537
0.755GlyMet: 0.755 ± 0.277
3.321GlyAsn: 3.321 ± 0.583
2.189GlyPro: 2.189 ± 0.345
1.962GlyGln: 1.962 ± 0.288
1.887GlyArg: 1.887 ± 0.249
5.811GlySer: 5.811 ± 0.697
3.019GlyThr: 3.019 ± 0.43
8.754GlyVal: 8.754 ± 0.871
0.679GlyTrp: 0.679 ± 0.325
2.339GlyTyr: 2.339 ± 0.232
0.0GlyXaa: 0.0 ± 0.0
His
0.755HisAla: 0.755 ± 0.396
0.151HisCys: 0.151 ± 0.096
0.604HisAsp: 0.604 ± 0.186
0.981HisGlu: 0.981 ± 0.34
0.755HisPhe: 0.755 ± 0.348
0.981HisGly: 0.981 ± 0.239
0.075HisHis: 0.075 ± 0.048
0.377HisIle: 0.377 ± 0.131
1.509HisLys: 1.509 ± 0.211
1.66HisLeu: 1.66 ± 0.555
0.226HisMet: 0.226 ± 0.118
1.207HisAsn: 1.207 ± 0.262
0.604HisPro: 0.604 ± 0.162
0.377HisGln: 0.377 ± 0.131
0.755HisArg: 0.755 ± 0.162
1.132HisSer: 1.132 ± 0.854
1.207HisThr: 1.207 ± 0.296
1.962HisVal: 1.962 ± 0.308
0.151HisTrp: 0.151 ± 0.057
0.906HisTyr: 0.906 ± 0.287
0.0HisXaa: 0.0 ± 0.0
Ile
3.773IleAla: 3.773 ± 0.521
1.811IleCys: 1.811 ± 0.252
3.019IleAsp: 3.019 ± 0.425
1.509IleGlu: 1.509 ± 0.568
2.189IlePhe: 2.189 ± 0.334
2.566IleGly: 2.566 ± 0.496
0.528IleHis: 0.528 ± 0.281
1.811IleIle: 1.811 ± 1.202
2.868IleLys: 2.868 ± 0.419
4.754IleLeu: 4.754 ± 0.491
0.981IleMet: 0.981 ± 0.247
2.641IleAsn: 2.641 ± 0.431
1.132IlePro: 1.132 ± 0.76
1.207IleGln: 1.207 ± 0.561
1.66IleArg: 1.66 ± 0.328
4.151IleSer: 4.151 ± 0.493
2.943IleThr: 2.943 ± 0.303
4.075IleVal: 4.075 ± 0.456
0.679IleTrp: 0.679 ± 0.24
2.641IleTyr: 2.641 ± 0.439
0.0IleXaa: 0.0 ± 0.0
Lys
3.019LysAla: 3.019 ± 0.641
2.49LysCys: 2.49 ± 0.377
2.717LysAsp: 2.717 ± 0.352
2.641LysGlu: 2.641 ± 0.368
3.094LysPhe: 3.094 ± 0.626
4.075LysGly: 4.075 ± 0.554
2.49LysHis: 2.49 ± 0.645
2.415LysIle: 2.415 ± 0.557
1.962LysLys: 1.962 ± 0.527
6.867LysLeu: 6.867 ± 0.562
1.057LysMet: 1.057 ± 0.301
2.189LysAsn: 2.189 ± 0.368
3.773LysPro: 3.773 ± 0.591
2.943LysGln: 2.943 ± 0.416
2.49LysArg: 2.49 ± 0.533
4.603LysSer: 4.603 ± 0.724
3.019LysThr: 3.019 ± 0.19
4.452LysVal: 4.452 ± 0.35
0.679LysTrp: 0.679 ± 0.239
2.49LysTyr: 2.49 ± 0.295
0.0LysXaa: 0.0 ± 0.0
Leu
5.584LeuAla: 5.584 ± 0.536
3.321LeuCys: 3.321 ± 0.517
4.151LeuAsp: 4.151 ± 0.507
3.547LeuGlu: 3.547 ± 0.409
5.358LeuPhe: 5.358 ± 1.16
5.283LeuGly: 5.283 ± 0.596
1.585LeuHis: 1.585 ± 0.249
3.547LeuIle: 3.547 ± 1.035
6.716LeuLys: 6.716 ± 0.935
10.188LeuLeu: 10.188 ± 1.605
0.981LeuMet: 0.981 ± 0.369
4.528LeuAsn: 4.528 ± 0.642
3.471LeuPro: 3.471 ± 1.578
4.603LeuGln: 4.603 ± 0.346
3.773LeuArg: 3.773 ± 0.37
8.83LeuSer: 8.83 ± 0.759
6.566LeuThr: 6.566 ± 0.68
7.018LeuVal: 7.018 ± 1.879
1.358LeuTrp: 1.358 ± 1.084
3.773LeuTyr: 3.773 ± 1.662
0.0LeuXaa: 0.0 ± 0.0
Met
1.132MetAla: 1.132 ± 0.24
0.981MetCys: 0.981 ± 0.28
1.057MetAsp: 1.057 ± 0.291
0.679MetGlu: 0.679 ± 0.241
1.283MetPhe: 1.283 ± 0.496
0.981MetGly: 0.981 ± 0.407
0.302MetHis: 0.302 ± 0.114
0.755MetIle: 0.755 ± 0.279
0.453MetLys: 0.453 ± 0.094
3.019MetLeu: 3.019 ± 0.558
0.226MetMet: 0.226 ± 0.143
0.981MetAsn: 0.981 ± 0.305
1.207MetPro: 1.207 ± 0.334
0.226MetGln: 0.226 ± 0.198
1.057MetArg: 1.057 ± 0.341
1.358MetSer: 1.358 ± 0.701
0.755MetThr: 0.755 ± 0.659
1.283MetVal: 1.283 ± 0.198
0.0MetTrp: 0.0 ± 0.0
1.585MetTyr: 1.585 ± 0.197
0.0MetXaa: 0.0 ± 0.0
Asn
4.075AsnAla: 4.075 ± 0.496
1.962AsnCys: 1.962 ± 0.394
2.113AsnAsp: 2.113 ± 0.427
2.415AsnGlu: 2.415 ± 0.478
3.17AsnPhe: 3.17 ± 0.629
5.132AsnGly: 5.132 ± 0.64
0.377AsnHis: 0.377 ± 0.163
2.566AsnIle: 2.566 ± 0.565
4.226AsnLys: 4.226 ± 0.588
4.075AsnLeu: 4.075 ± 0.388
0.83AsnMet: 0.83 ± 0.409
2.415AsnAsn: 2.415 ± 0.38
1.207AsnPro: 1.207 ± 0.334
1.509AsnGln: 1.509 ± 0.24
1.057AsnArg: 1.057 ± 0.277
5.207AsnSer: 5.207 ± 0.927
3.094AsnThr: 3.094 ± 0.613
7.547AsnVal: 7.547 ± 0.77
0.453AsnTrp: 0.453 ± 0.608
1.736AsnTyr: 1.736 ± 0.613
0.0AsnXaa: 0.0 ± 0.0
Pro
3.019ProAla: 3.019 ± 0.395
0.83ProCys: 0.83 ± 0.189
1.358ProAsp: 1.358 ± 0.652
1.736ProGlu: 1.736 ± 0.255
2.566ProPhe: 2.566 ± 0.66
2.264ProGly: 2.264 ± 0.435
0.302ProHis: 0.302 ± 0.119
1.66ProIle: 1.66 ± 0.378
1.962ProLys: 1.962 ± 0.946
2.943ProLeu: 2.943 ± 0.58
0.453ProMet: 0.453 ± 0.328
1.283ProAsn: 1.283 ± 0.341
1.207ProPro: 1.207 ± 0.505
1.283ProGln: 1.283 ± 0.262
1.358ProArg: 1.358 ± 0.751
3.094ProSer: 3.094 ± 0.457
2.641ProThr: 2.641 ± 0.289
4.452ProVal: 4.452 ± 0.631
0.604ProTrp: 0.604 ± 0.12
1.283ProTyr: 1.283 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
2.868GlnAla: 2.868 ± 0.438
0.302GlnCys: 0.302 ± 0.119
0.906GlnAsp: 0.906 ± 0.285
1.283GlnGlu: 1.283 ± 0.368
1.057GlnPhe: 1.057 ± 0.558
1.66GlnGly: 1.66 ± 0.263
0.528GlnHis: 0.528 ± 0.107
1.434GlnIle: 1.434 ± 0.526
1.811GlnLys: 1.811 ± 0.465
4.151GlnLeu: 4.151 ± 1.044
0.83GlnMet: 0.83 ± 0.195
1.358GlnAsn: 1.358 ± 0.331
2.113GlnPro: 2.113 ± 0.392
0.604GlnGln: 0.604 ± 0.233
0.83GlnArg: 0.83 ± 0.319
1.962GlnSer: 1.962 ± 0.248
1.811GlnThr: 1.811 ± 0.947
2.189GlnVal: 2.189 ± 0.697
0.528GlnTrp: 0.528 ± 0.185
0.981GlnTyr: 0.981 ± 0.773
0.0GlnXaa: 0.0 ± 0.0
Arg
3.094ArgAla: 3.094 ± 0.39
1.509ArgCys: 1.509 ± 0.421
2.113ArgAsp: 2.113 ± 0.255
1.057ArgGlu: 1.057 ± 0.523
3.019ArgPhe: 3.019 ± 0.773
1.736ArgGly: 1.736 ± 0.513
0.528ArgHis: 0.528 ± 0.185
1.207ArgIle: 1.207 ± 0.181
2.339ArgLys: 2.339 ± 0.346
3.547ArgLeu: 3.547 ± 0.516
0.604ArgMet: 0.604 ± 0.162
1.887ArgAsn: 1.887 ± 0.415
1.358ArgPro: 1.358 ± 0.348
0.453ArgGln: 0.453 ± 0.141
0.83ArgArg: 0.83 ± 0.334
1.585ArgSer: 1.585 ± 1.001
2.264ArgThr: 2.264 ± 0.59
3.547ArgVal: 3.547 ± 0.806
0.453ArgTrp: 0.453 ± 0.145
1.283ArgTyr: 1.283 ± 0.262
0.0ArgXaa: 0.0 ± 0.0
Ser
4.905SerAla: 4.905 ± 0.47
1.283SerCys: 1.283 ± 0.536
6.264SerAsp: 6.264 ± 0.971
3.396SerGlu: 3.396 ± 0.481
4.754SerPhe: 4.754 ± 0.77
5.584SerGly: 5.584 ± 0.541
0.755SerHis: 0.755 ± 0.206
4.528SerIle: 4.528 ± 0.56
4.226SerLys: 4.226 ± 0.626
6.792SerLeu: 6.792 ± 1.368
1.887SerMet: 1.887 ± 0.421
3.849SerAsn: 3.849 ± 0.784
1.962SerPro: 1.962 ± 0.351
1.585SerGln: 1.585 ± 0.687
2.641SerArg: 2.641 ± 1.504
4.377SerSer: 4.377 ± 2.087
5.056SerThr: 5.056 ± 1.078
7.32SerVal: 7.32 ± 1.096
0.377SerTrp: 0.377 ± 0.36
3.622SerTyr: 3.622 ± 1.121
0.0SerXaa: 0.0 ± 0.0
Thr
3.396ThrAla: 3.396 ± 0.717
1.132ThrCys: 1.132 ± 0.174
1.887ThrAsp: 1.887 ± 0.584
1.811ThrGlu: 1.811 ± 0.167
2.717ThrPhe: 2.717 ± 0.291
3.245ThrGly: 3.245 ± 0.786
0.906ThrHis: 0.906 ± 0.206
3.396ThrIle: 3.396 ± 0.409
4.0ThrLys: 4.0 ± 0.619
6.339ThrLeu: 6.339 ± 0.654
1.811ThrMet: 1.811 ± 0.3
3.471ThrAsn: 3.471 ± 0.363
2.113ThrPro: 2.113 ± 0.212
2.49ThrGln: 2.49 ± 0.792
1.585ThrArg: 1.585 ± 0.509
5.283ThrSer: 5.283 ± 1.185
4.377ThrThr: 4.377 ± 0.505
5.283ThrVal: 5.283 ± 0.754
0.604ThrTrp: 0.604 ± 0.18
1.66ThrTyr: 1.66 ± 0.407
0.0ThrXaa: 0.0 ± 0.0
Val
7.169ValAla: 7.169 ± 0.617
3.622ValCys: 3.622 ± 0.899
6.339ValAsp: 6.339 ± 0.781
4.302ValGlu: 4.302 ± 0.531
4.452ValPhe: 4.452 ± 0.561
4.075ValGly: 4.075 ± 0.711
1.434ValHis: 1.434 ± 0.226
4.679ValIle: 4.679 ± 0.771
6.716ValLys: 6.716 ± 1.216
8.679ValLeu: 8.679 ± 1.687
2.113ValMet: 2.113 ± 0.778
5.584ValAsn: 5.584 ± 0.312
4.377ValPro: 4.377 ± 0.388
3.698ValGln: 3.698 ± 0.878
3.924ValArg: 3.924 ± 0.413
6.943ValSer: 6.943 ± 1.448
5.509ValThr: 5.509 ± 1.658
10.943ValVal: 10.943 ± 1.75
1.057ValTrp: 1.057 ± 0.184
3.698ValTyr: 3.698 ± 0.971
0.0ValXaa: 0.0 ± 0.0
Trp
0.906TrpAla: 0.906 ± 1.214
0.377TrpCys: 0.377 ± 0.131
0.906TrpAsp: 0.906 ± 0.2
0.679TrpGlu: 0.679 ± 0.24
0.83TrpPhe: 0.83 ± 0.21
0.302TrpGly: 0.302 ± 0.119
0.302TrpHis: 0.302 ± 0.114
0.83TrpIle: 0.83 ± 0.319
0.528TrpLys: 0.528 ± 0.139
1.811TrpLeu: 1.811 ± 0.321
0.075TrpMet: 0.075 ± 0.048
0.755TrpAsn: 0.755 ± 0.877
0.604TrpPro: 0.604 ± 0.35
0.075TrpGln: 0.075 ± 0.048
0.604TrpArg: 0.604 ± 0.163
0.604TrpSer: 0.604 ± 0.196
0.453TrpThr: 0.453 ± 0.133
0.755TrpVal: 0.755 ± 0.329
0.377TrpTrp: 0.377 ± 0.103
0.906TrpTyr: 0.906 ± 0.24
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.396TyrAla: 3.396 ± 0.678
1.509TyrCys: 1.509 ± 0.308
3.17TyrAsp: 3.17 ± 0.78
2.189TyrGlu: 2.189 ± 0.457
2.415TyrPhe: 2.415 ± 0.849
2.339TyrGly: 2.339 ± 0.694
0.377TyrHis: 0.377 ± 0.258
2.113TyrIle: 2.113 ± 0.422
2.264TyrLys: 2.264 ± 0.308
4.0TyrLeu: 4.0 ± 0.799
1.132TyrMet: 1.132 ± 0.297
4.151TyrAsn: 4.151 ± 0.791
1.057TyrPro: 1.057 ± 0.34
0.981TyrGln: 0.981 ± 0.49
1.132TyrArg: 1.132 ± 0.319
2.717TyrSer: 2.717 ± 0.256
2.566TyrThr: 2.566 ± 0.51
3.773TyrVal: 3.773 ± 0.999
0.453TyrTrp: 0.453 ± 0.13
2.038TyrTyr: 2.038 ± 0.425
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.151XaaAla: 0.151 ± 0.057
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8 proteins (13252 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski