Paenibacillus sp. N4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5080 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A559ID85|A0A559ID85_9BACL Uncharacterized protein OS=Paenibacillus sp. N4 OX=2590547 GN=FPZ44_24950 PE=4 SV=1
MM1 pKa = 7.56SIKK4 pKa = 10.44EE5 pKa = 3.96NEE7 pKa = 3.9ISNGCGTRR15 pKa = 11.84GMLQEE20 pKa = 4.21TVLLYY25 pKa = 10.69KK26 pKa = 8.25WTSYY30 pKa = 11.22FDD32 pKa = 4.47DD33 pKa = 5.06GDD35 pKa = 3.83VLVIFMLRR43 pKa = 11.84PVCEE47 pKa = 4.77DD48 pKa = 3.59DD49 pKa = 3.7STDD52 pKa = 3.91GGVIDD57 pKa = 5.91DD58 pKa = 4.36GGKK61 pKa = 10.39LYY63 pKa = 10.5IMPQGYY69 pKa = 9.19SLEE72 pKa = 4.09TSRR75 pKa = 11.84FSGEE79 pKa = 3.85KK80 pKa = 10.53YY81 pKa = 10.18IVDD84 pKa = 4.44TIGTSVEE91 pKa = 4.29YY92 pKa = 10.19IDD94 pKa = 4.94MDD96 pKa = 4.79DD97 pKa = 3.83NGMPVLMSIEE107 pKa = 4.06GDD109 pKa = 3.42KK110 pKa = 10.96VLCLAA115 pKa = 5.1

Molecular weight: 12.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A559IQE3|A0A559IQE3_9BACL Iron-hydroxamate ABC transporter substrate-binding protein OS=Paenibacillus sp. N4 OX=2590547 GN=FPZ44_18185 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.88RR12 pKa = 11.84KK13 pKa = 8.74KK14 pKa = 8.68NHH16 pKa = 4.64GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.58GRR39 pKa = 11.84AKK41 pKa = 10.7LSAA44 pKa = 3.92

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5080

0

5080

1629143

26

5582

320.7

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.799 ± 0.037

0.788 ± 0.011

5.14 ± 0.028

6.78 ± 0.039

3.977 ± 0.024

6.844 ± 0.033

2.322 ± 0.022

6.973 ± 0.032

5.595 ± 0.039

9.596 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.895 ± 0.02

4.128 ± 0.03

3.813 ± 0.023

4.308 ± 0.025

4.753 ± 0.032

6.423 ± 0.031

5.681 ± 0.043

7.329 ± 0.03

1.244 ± 0.016

3.61 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski