Amino acid dipepetide frequency for Paenibacillus sp. N4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.554AlaAla: 7.554 ± 0.095
0.716AlaCys: 0.716 ± 0.021
4.035AlaAsp: 4.035 ± 0.056
5.204AlaGlu: 5.204 ± 0.076
3.015AlaPhe: 3.015 ± 0.056
5.786AlaGly: 5.786 ± 0.068
1.592AlaHis: 1.592 ± 0.035
5.434AlaIle: 5.434 ± 0.067
4.274AlaLys: 4.274 ± 0.059
7.673AlaLeu: 7.673 ± 0.085
2.167AlaMet: 2.167 ± 0.039
3.018AlaAsn: 3.018 ± 0.048
2.584AlaPro: 2.584 ± 0.051
2.94AlaGln: 2.94 ± 0.042
3.278AlaArg: 3.278 ± 0.046
4.985AlaSer: 4.985 ± 0.063
3.899AlaThr: 3.899 ± 0.076
6.184AlaVal: 6.184 ± 0.079
0.913AlaTrp: 0.913 ± 0.03
2.744AlaTyr: 2.744 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.524CysAla: 0.524 ± 0.018
0.117CysCys: 0.117 ± 0.008
0.393CysAsp: 0.393 ± 0.016
0.466CysGlu: 0.466 ± 0.019
0.336CysPhe: 0.336 ± 0.014
0.697CysGly: 0.697 ± 0.022
0.201CysHis: 0.201 ± 0.011
0.545CysIle: 0.545 ± 0.018
0.406CysLys: 0.406 ± 0.016
0.692CysLeu: 0.692 ± 0.022
0.238CysMet: 0.238 ± 0.012
0.303CysAsn: 0.303 ± 0.014
0.347CysPro: 0.347 ± 0.016
0.259CysGln: 0.259 ± 0.015
0.409CysArg: 0.409 ± 0.016
0.6CysSer: 0.6 ± 0.017
0.479CysThr: 0.479 ± 0.016
0.484CysVal: 0.484 ± 0.016
0.092CysTrp: 0.092 ± 0.008
0.29CysTyr: 0.29 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.863AspAla: 3.863 ± 0.052
0.395AspCys: 0.395 ± 0.017
2.554AspAsp: 2.554 ± 0.053
3.957AspGlu: 3.957 ± 0.051
1.986AspPhe: 1.986 ± 0.039
3.707AspGly: 3.707 ± 0.065
1.123AspHis: 1.123 ± 0.03
3.78AspIle: 3.78 ± 0.05
3.056AspLys: 3.056 ± 0.044
4.534AspLeu: 4.534 ± 0.053
1.505AspMet: 1.505 ± 0.026
2.035AspAsn: 2.035 ± 0.04
2.067AspPro: 2.067 ± 0.042
1.882AspGln: 1.882 ± 0.033
2.605AspArg: 2.605 ± 0.044
2.916AspSer: 2.916 ± 0.045
2.674AspThr: 2.674 ± 0.039
3.928AspVal: 3.928 ± 0.056
0.794AspTrp: 0.794 ± 0.024
2.043AspTyr: 2.043 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
5.804GluAla: 5.804 ± 0.079
0.424GluCys: 0.424 ± 0.018
3.113GluAsp: 3.113 ± 0.048
5.193GluGlu: 5.193 ± 0.069
2.108GluPhe: 2.108 ± 0.042
4.117GluGly: 4.117 ± 0.065
1.922GluHis: 1.922 ± 0.051
4.138GluIle: 4.138 ± 0.058
4.048GluLys: 4.048 ± 0.065
6.924GluLeu: 6.924 ± 0.065
2.132GluMet: 2.132 ± 0.042
2.379GluAsn: 2.379 ± 0.045
2.074GluPro: 2.074 ± 0.038
4.693GluGln: 4.693 ± 0.061
4.216GluArg: 4.216 ± 0.056
3.485GluSer: 3.485 ± 0.049
3.259GluThr: 3.259 ± 0.046
4.717GluVal: 4.717 ± 0.07
0.958GluTrp: 0.958 ± 0.026
2.102GluTyr: 2.102 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
2.939PheAla: 2.939 ± 0.044
0.349PheCys: 0.349 ± 0.016
2.342PheAsp: 2.342 ± 0.042
2.591PheGlu: 2.591 ± 0.044
1.626PhePhe: 1.626 ± 0.041
2.908PheGly: 2.908 ± 0.045
0.907PheHis: 0.907 ± 0.026
3.123PheIle: 3.123 ± 0.049
2.0PheLys: 2.0 ± 0.041
3.331PheLeu: 3.331 ± 0.059
1.215PheMet: 1.215 ± 0.028
1.689PheAsn: 1.689 ± 0.034
1.411PhePro: 1.411 ± 0.033
1.469PheGln: 1.469 ± 0.029
1.676PheArg: 1.676 ± 0.032
2.688PheSer: 2.688 ± 0.045
2.455PheThr: 2.455 ± 0.046
3.106PheVal: 3.106 ± 0.045
0.472PheTrp: 0.472 ± 0.016
1.468PheTyr: 1.468 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
5.232GlyAla: 5.232 ± 0.086
0.655GlyCys: 0.655 ± 0.022
3.229GlyAsp: 3.229 ± 0.052
4.163GlyGlu: 4.163 ± 0.059
2.948GlyPhe: 2.948 ± 0.048
4.801GlyGly: 4.801 ± 0.065
1.483GlyHis: 1.483 ± 0.031
5.282GlyIle: 5.282 ± 0.057
4.212GlyLys: 4.212 ± 0.057
6.294GlyLeu: 6.294 ± 0.073
2.148GlyMet: 2.148 ± 0.035
2.727GlyAsn: 2.727 ± 0.054
1.744GlyPro: 1.744 ± 0.044
2.695GlyGln: 2.695 ± 0.056
3.029GlyArg: 3.029 ± 0.054
4.362GlySer: 4.362 ± 0.058
4.405GlyThr: 4.405 ± 0.07
5.237GlyVal: 5.237 ± 0.072
0.931GlyTrp: 0.931 ± 0.025
2.865GlyTyr: 2.865 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
1.742HisAla: 1.742 ± 0.039
0.195HisCys: 0.195 ± 0.011
1.105HisAsp: 1.105 ± 0.023
1.48HisGlu: 1.48 ± 0.032
1.017HisPhe: 1.017 ± 0.024
1.509HisGly: 1.509 ± 0.034
0.746HisHis: 0.746 ± 0.023
1.779HisIle: 1.779 ± 0.053
1.019HisLys: 1.019 ± 0.03
2.232HisLeu: 2.232 ± 0.061
0.656HisMet: 0.656 ± 0.021
0.896HisAsn: 0.896 ± 0.025
1.107HisPro: 1.107 ± 0.029
0.956HisGln: 0.956 ± 0.025
1.099HisArg: 1.099 ± 0.028
1.417HisSer: 1.417 ± 0.035
1.288HisThr: 1.288 ± 0.03
1.74HisVal: 1.74 ± 0.037
0.303HisTrp: 0.303 ± 0.015
0.934HisTyr: 0.934 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.92IleAla: 5.92 ± 0.079
0.659IleCys: 0.659 ± 0.023
3.918IleAsp: 3.918 ± 0.054
4.935IleGlu: 4.935 ± 0.063
2.434IlePhe: 2.434 ± 0.046
5.38IleGly: 5.38 ± 0.071
1.686IleHis: 1.686 ± 0.034
4.703IleIle: 4.703 ± 0.072
3.297IleLys: 3.297 ± 0.058
5.451IleLeu: 5.451 ± 0.066
1.762IleMet: 1.762 ± 0.04
2.7IleAsn: 2.7 ± 0.047
3.127IlePro: 3.127 ± 0.052
2.975IleGln: 2.975 ± 0.049
3.534IleArg: 3.534 ± 0.049
4.606IleSer: 4.606 ± 0.061
4.117IleThr: 4.117 ± 0.061
5.722IleVal: 5.722 ± 0.066
0.71IleTrp: 0.71 ± 0.025
2.091IleTyr: 2.091 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.04LysAla: 4.04 ± 0.051
0.295LysCys: 0.295 ± 0.013
3.09LysAsp: 3.09 ± 0.057
4.34LysGlu: 4.34 ± 0.06
1.609LysPhe: 1.609 ± 0.04
3.564LysGly: 3.564 ± 0.056
1.403LysHis: 1.403 ± 0.031
3.035LysIle: 3.035 ± 0.055
3.585LysLys: 3.585 ± 0.075
5.718LysLeu: 5.718 ± 0.074
1.8LysMet: 1.8 ± 0.037
2.118LysAsn: 2.118 ± 0.042
2.352LysPro: 2.352 ± 0.052
3.204LysGln: 3.204 ± 0.044
3.066LysArg: 3.066 ± 0.05
3.295LysSer: 3.295 ± 0.055
2.718LysThr: 2.718 ± 0.041
4.005LysVal: 4.005 ± 0.061
0.799LysTrp: 0.799 ± 0.024
1.916LysTyr: 1.916 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
7.568LeuAla: 7.568 ± 0.078
0.799LeuCys: 0.799 ± 0.022
4.933LeuAsp: 4.933 ± 0.063
6.084LeuGlu: 6.084 ± 0.069
4.355LeuPhe: 4.355 ± 0.06
6.105LeuGly: 6.105 ± 0.074
2.256LeuHis: 2.256 ± 0.039
6.34LeuIle: 6.34 ± 0.081
5.36LeuLys: 5.36 ± 0.067
10.259LeuLeu: 10.259 ± 0.12
2.421LeuMet: 2.421 ± 0.037
3.914LeuAsn: 3.914 ± 0.054
4.118LeuPro: 4.118 ± 0.058
4.184LeuGln: 4.184 ± 0.051
4.455LeuArg: 4.455 ± 0.058
6.77LeuSer: 6.77 ± 0.067
5.632LeuThr: 5.632 ± 0.064
6.243LeuVal: 6.243 ± 0.061
0.974LeuTrp: 0.974 ± 0.027
3.19LeuTyr: 3.19 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
2.081MetAla: 2.081 ± 0.037
0.188MetCys: 0.188 ± 0.011
1.453MetAsp: 1.453 ± 0.03
1.942MetGlu: 1.942 ± 0.04
1.12MetPhe: 1.12 ± 0.029
1.609MetGly: 1.609 ± 0.038
0.53MetHis: 0.53 ± 0.018
2.021MetIle: 2.021 ± 0.038
2.183MetLys: 2.183 ± 0.042
2.999MetLeu: 2.999 ± 0.049
1.01MetMet: 1.01 ± 0.024
1.635MetAsn: 1.635 ± 0.029
1.119MetPro: 1.119 ± 0.026
1.157MetGln: 1.157 ± 0.026
1.366MetArg: 1.366 ± 0.029
1.978MetSer: 1.978 ± 0.035
1.757MetThr: 1.757 ± 0.037
1.667MetVal: 1.667 ± 0.036
0.255MetTrp: 0.255 ± 0.012
0.882MetTyr: 0.882 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
2.835AsnAla: 2.835 ± 0.045
0.322AsnCys: 0.322 ± 0.013
2.215AsnAsp: 2.215 ± 0.041
2.986AsnGlu: 2.986 ± 0.046
1.354AsnPhe: 1.354 ± 0.032
3.405AsnGly: 3.405 ± 0.061
1.001AsnHis: 1.001 ± 0.024
2.673AsnIle: 2.673 ± 0.042
2.577AsnLys: 2.577 ± 0.043
3.202AsnLeu: 3.202 ± 0.043
1.166AsnMet: 1.166 ± 0.028
1.933AsnAsn: 1.933 ± 0.045
1.977AsnPro: 1.977 ± 0.037
1.805AsnGln: 1.805 ± 0.037
2.14AsnArg: 2.14 ± 0.039
2.313AsnSer: 2.313 ± 0.046
2.243AsnThr: 2.243 ± 0.062
3.105AsnVal: 3.105 ± 0.064
0.541AsnTrp: 0.541 ± 0.02
1.491AsnTyr: 1.491 ± 0.032
0.0AsnXaa: 0.0 ± 0.0
Pro
2.895ProAla: 2.895 ± 0.047
0.241ProCys: 0.241 ± 0.012
2.275ProAsp: 2.275 ± 0.041
2.819ProGlu: 2.819 ± 0.045
1.784ProPhe: 1.784 ± 0.035
2.358ProGly: 2.358 ± 0.055
0.927ProHis: 0.927 ± 0.026
2.814ProIle: 2.814 ± 0.045
1.791ProLys: 1.791 ± 0.035
3.754ProLeu: 3.754 ± 0.057
0.858ProMet: 0.858 ± 0.024
1.862ProAsn: 1.862 ± 0.039
1.202ProPro: 1.202 ± 0.031
1.43ProGln: 1.43 ± 0.033
1.314ProArg: 1.314 ± 0.029
2.647ProSer: 2.647 ± 0.046
2.324ProThr: 2.324 ± 0.047
2.827ProVal: 2.827 ± 0.044
0.446ProTrp: 0.446 ± 0.019
1.562ProTyr: 1.562 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.758GlnAla: 3.758 ± 0.054
0.246GlnCys: 0.246 ± 0.012
1.888GlnAsp: 1.888 ± 0.037
2.801GlnGlu: 2.801 ± 0.046
1.75GlnPhe: 1.75 ± 0.038
2.579GlnGly: 2.579 ± 0.058
1.171GlnHis: 1.171 ± 0.027
2.604GlnIle: 2.604 ± 0.042
1.926GlnLys: 1.926 ± 0.039
5.226GlnLeu: 5.226 ± 0.064
1.317GlnMet: 1.317 ± 0.032
1.336GlnAsn: 1.336 ± 0.031
1.683GlnPro: 1.683 ± 0.032
2.599GlnGln: 2.599 ± 0.049
2.068GlnArg: 2.068 ± 0.038
2.704GlnSer: 2.704 ± 0.046
2.164GlnThr: 2.164 ± 0.043
3.056GlnVal: 3.056 ± 0.045
0.61GlnTrp: 0.61 ± 0.02
1.592GlnTyr: 1.592 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
3.262ArgAla: 3.262 ± 0.055
0.36ArgCys: 0.36 ± 0.017
2.305ArgAsp: 2.305 ± 0.042
3.378ArgGlu: 3.378 ± 0.047
1.951ArgPhe: 1.951 ± 0.033
2.638ArgGly: 2.638 ± 0.047
1.046ArgHis: 1.046 ± 0.027
3.714ArgIle: 3.714 ± 0.057
2.889ArgLys: 2.889 ± 0.045
4.771ArgLeu: 4.771 ± 0.071
1.616ArgMet: 1.616 ± 0.031
2.129ArgAsn: 2.129 ± 0.033
1.577ArgPro: 1.577 ± 0.037
1.977ArgGln: 1.977 ± 0.037
2.371ArgArg: 2.371 ± 0.046
2.974ArgSer: 2.974 ± 0.04
2.75ArgThr: 2.75 ± 0.048
3.277ArgVal: 3.277 ± 0.044
0.672ArgTrp: 0.672 ± 0.023
1.878ArgTyr: 1.878 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
4.539SerAla: 4.539 ± 0.065
0.479SerCys: 0.479 ± 0.018
3.04SerAsp: 3.04 ± 0.049
3.838SerGlu: 3.838 ± 0.057
3.048SerPhe: 3.048 ± 0.051
4.822SerGly: 4.822 ± 0.063
1.396SerHis: 1.396 ± 0.034
4.973SerIle: 4.973 ± 0.065
3.593SerLys: 3.593 ± 0.053
6.144SerLeu: 6.144 ± 0.072
1.881SerMet: 1.881 ± 0.031
2.915SerAsn: 2.915 ± 0.054
2.232SerPro: 2.232 ± 0.036
2.266SerGln: 2.266 ± 0.035
2.752SerArg: 2.752 ± 0.046
4.715SerSer: 4.715 ± 0.066
3.728SerThr: 3.728 ± 0.053
4.526SerVal: 4.526 ± 0.064
0.797SerTrp: 0.797 ± 0.025
2.547SerTyr: 2.547 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
4.702ThrAla: 4.702 ± 0.068
0.39ThrCys: 0.39 ± 0.017
2.806ThrAsp: 2.806 ± 0.046
3.385ThrGlu: 3.385 ± 0.047
2.494ThrPhe: 2.494 ± 0.046
4.464ThrGly: 4.464 ± 0.125
1.137ThrHis: 1.137 ± 0.025
4.373ThrIle: 4.373 ± 0.069
2.88ThrLys: 2.88 ± 0.05
5.41ThrLeu: 5.41 ± 0.064
1.493ThrMet: 1.493 ± 0.032
2.406ThrAsn: 2.406 ± 0.059
2.457ThrPro: 2.457 ± 0.047
1.705ThrGln: 1.705 ± 0.038
2.135ThrArg: 2.135 ± 0.037
3.834ThrSer: 3.834 ± 0.065
3.384ThrThr: 3.384 ± 0.057
4.468ThrVal: 4.468 ± 0.092
0.67ThrTrp: 0.67 ± 0.02
2.218ThrTyr: 2.218 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
5.283ValAla: 5.283 ± 0.064
0.645ValCys: 0.645 ± 0.019
3.93ValAsp: 3.93 ± 0.045
4.876ValGlu: 4.876 ± 0.061
2.769ValPhe: 2.769 ± 0.042
4.7ValGly: 4.7 ± 0.063
1.592ValHis: 1.592 ± 0.034
5.195ValIle: 5.195 ± 0.061
4.17ValLys: 4.17 ± 0.05
6.921ValLeu: 6.921 ± 0.074
2.062ValMet: 2.062 ± 0.036
3.121ValAsn: 3.121 ± 0.062
3.154ValPro: 3.154 ± 0.059
3.011ValGln: 3.011 ± 0.041
3.416ValArg: 3.416 ± 0.047
4.898ValSer: 4.898 ± 0.07
4.703ValThr: 4.703 ± 0.1
5.555ValVal: 5.555 ± 0.072
0.848ValTrp: 0.848 ± 0.025
2.439ValTyr: 2.439 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
0.807TrpAla: 0.807 ± 0.024
0.087TrpCys: 0.087 ± 0.007
0.662TrpAsp: 0.662 ± 0.024
0.758TrpGlu: 0.758 ± 0.025
0.549TrpPhe: 0.549 ± 0.02
0.783TrpGly: 0.783 ± 0.025
0.28TrpHis: 0.28 ± 0.014
0.912TrpIle: 0.912 ± 0.026
0.806TrpLys: 0.806 ± 0.025
1.361TrpLeu: 1.361 ± 0.031
0.475TrpMet: 0.475 ± 0.019
0.729TrpAsn: 0.729 ± 0.021
0.304TrpPro: 0.304 ± 0.014
0.485TrpGln: 0.485 ± 0.019
0.58TrpArg: 0.58 ± 0.02
0.824TrpSer: 0.824 ± 0.024
0.692TrpThr: 0.692 ± 0.025
0.748TrpVal: 0.748 ± 0.021
0.196TrpTrp: 0.196 ± 0.011
0.404TrpTyr: 0.404 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.646TyrAla: 2.646 ± 0.046
0.317TyrCys: 0.317 ± 0.013
2.119TyrAsp: 2.119 ± 0.056
2.607TyrGlu: 2.607 ± 0.038
1.514TyrPhe: 1.514 ± 0.034
2.506TyrGly: 2.506 ± 0.042
0.821TyrHis: 0.821 ± 0.024
2.291TyrIle: 2.291 ± 0.038
1.881TyrLys: 1.881 ± 0.038
3.065TyrLeu: 3.065 ± 0.051
1.031TyrMet: 1.031 ± 0.024
1.44TyrAsn: 1.44 ± 0.038
1.495TyrPro: 1.495 ± 0.033
1.387TyrGln: 1.387 ± 0.032
2.024TyrArg: 2.024 ± 0.04
2.22TyrSer: 2.22 ± 0.044
2.14TyrThr: 2.14 ± 0.039
2.695TyrVal: 2.695 ± 0.044
0.459TyrTrp: 0.459 ± 0.017
1.444TyrTyr: 1.444 ± 0.037
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5080 proteins (1629143 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski