Rhodnius prolixus (Triatomid bug)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Hemiptera; Prosorrhyncha; Heteroptera; Euheteroptera; Neoheteroptera; Panheteroptera; Cimicomorpha;

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14941 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1HZ60|T1HZ60_RHOPR PKS_ER domain-containing protein OS=Rhodnius prolixus OX=13249 PE=3 SV=1
MM1 pKa = 7.42TEE3 pKa = 3.8IKK5 pKa = 10.55FEE7 pKa = 4.53LRR9 pKa = 11.84FCSIFALKK17 pKa = 9.9TSLDD21 pKa = 3.56ATTSSFDD28 pKa = 3.11ATTSYY33 pKa = 10.47ATTSSLDD40 pKa = 3.32ATTSSFDD47 pKa = 3.55ATPSSLDD54 pKa = 3.37ATTSSPDD61 pKa = 2.81ATTYY65 pKa = 10.79HH66 pKa = 6.65LNSLDD71 pKa = 3.41VTDD74 pKa = 5.54PYY76 pKa = 11.37AGEE79 pKa = 4.15LVV81 pKa = 3.37

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1ICL5|T1ICL5_RHOPR Uncharacterized protein OS=Rhodnius prolixus OX=13249 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 7.85RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.6MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14927

14

14941

5337290

8

11288

357.2

40.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.9 ± 0.021

2.08 ± 0.02

5.178 ± 0.016

6.744 ± 0.028

4.024 ± 0.018

5.594 ± 0.029

2.425 ± 0.012

6.019 ± 0.022

6.869 ± 0.03

9.656 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.282 ± 0.012

5.005 ± 0.021

4.828 ± 0.026

3.95 ± 0.019

5.057 ± 0.017

7.839 ± 0.026

5.69 ± 0.019

6.363 ± 0.018

1.15 ± 0.009

3.345 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski