Amino acid dipepetide frequency for Rhodnius prolixus (Triatomid bug)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.875AlaAla: 4.875 ± 0.053	1.136AlaCys: 1.136 ± 0.019	2.812AlaAsp: 2.812 ± 0.027	3.824AlaGlu: 3.824 ± 0.03	2.219AlaPhe: 2.219 ± 0.026	3.456AlaGly: 3.456 ± 0.036	1.359AlaHis: 1.359 ± 0.02	3.518AlaIle: 3.518 ± 0.027	3.675AlaLys: 3.675 ± 0.026	5.947AlaLeu: 5.947 ± 0.044	1.406AlaMet: 1.406 ± 0.018	2.526AlaAsn: 2.526 ± 0.023	2.583AlaPro: 2.583 ± 0.03	2.242AlaGln: 2.242 ± 0.023	2.877AlaArg: 2.877 ± 0.029	4.367AlaSer: 4.367 ± 0.033	3.492AlaThr: 3.492 ± 0.034	4.361AlaVal: 4.361 ± 0.038	0.632AlaTrp: 0.632 ± 0.012	1.692AlaTyr: 1.692 ± 0.019	0.0AlaXaa: 0.0 ± 0.0
Cys	1.095CysAla: 1.095 ± 0.018	0.492CysCys: 0.492 ± 0.013	1.142CysAsp: 1.142 ± 0.019	1.235CysGlu: 1.235 ± 0.022	0.829CysPhe: 0.829 ± 0.013	1.391CysGly: 1.391 ± 0.025	0.52CysHis: 0.52 ± 0.011	1.229CysIle: 1.229 ± 0.023	1.334CysLys: 1.334 ± 0.023	2.032CysLeu: 2.032 ± 0.026	0.412CysMet: 0.412 ± 0.009	1.051CysAsn: 1.051 ± 0.019	1.068CysPro: 1.068 ± 0.023	0.759CysGln: 0.759 ± 0.015	1.093CysArg: 1.093 ± 0.021	1.7CysSer: 1.7 ± 0.025	1.24CysThr: 1.24 ± 0.02	1.268CysVal: 1.268 ± 0.02	0.248CysTrp: 0.248 ± 0.008	0.662CysTyr: 0.662 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	2.579AspAla: 2.579 ± 0.026	1.096AspCys: 1.096 ± 0.02	3.295AspAsp: 3.295 ± 0.039	3.98AspGlu: 3.98 ± 0.033	2.223AspPhe: 2.223 ± 0.024	2.945AspGly: 2.945 ± 0.034	1.08AspHis: 1.08 ± 0.014	3.517AspIle: 3.517 ± 0.029	3.402AspLys: 3.402 ± 0.03	5.004AspLeu: 5.004 ± 0.038	1.202AspMet: 1.202 ± 0.015	2.517AspAsn: 2.517 ± 0.027	2.313AspPro: 2.313 ± 0.027	1.625AspGln: 1.625 ± 0.019	2.408AspArg: 2.408 ± 0.024	4.043AspSer: 4.043 ± 0.039	2.607AspThr: 2.607 ± 0.027	3.398AspVal: 3.398 ± 0.024	0.667AspTrp: 0.667 ± 0.014	1.884AspTyr: 1.884 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	3.938GluAla: 3.938 ± 0.037	1.332GluCys: 1.332 ± 0.027	4.031GluAsp: 4.031 ± 0.038	6.546GluGlu: 6.546 ± 0.07	2.346GluPhe: 2.346 ± 0.021	3.283GluGly: 3.283 ± 0.031	1.449GluHis: 1.449 ± 0.018	4.296GluIle: 4.296 ± 0.04	5.317GluLys: 5.317 ± 0.053	6.095GluLeu: 6.095 ± 0.042	1.74GluMet: 1.74 ± 0.023	3.735GluAsn: 3.735 ± 0.031	2.389GluPro: 2.389 ± 0.025	2.559GluGln: 2.559 ± 0.029	3.542GluArg: 3.542 ± 0.038	4.323GluSer: 4.323 ± 0.033	3.509GluThr: 3.509 ± 0.033	4.255GluVal: 4.255 ± 0.032	0.732GluTrp: 0.732 ± 0.013	2.022GluTyr: 2.022 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	2.111PheAla: 2.111 ± 0.025	0.905PheCys: 0.905 ± 0.014	2.057PheAsp: 2.057 ± 0.02	2.238PheGlu: 2.238 ± 0.026	1.8PhePhe: 1.8 ± 0.024	2.437PheGly: 2.437 ± 0.028	1.007PheHis: 1.007 ± 0.014	2.656PheIle: 2.656 ± 0.028	2.557PheLys: 2.557 ± 0.025	4.108PheLeu: 4.108 ± 0.035	0.895PheMet: 0.895 ± 0.015	2.027PheAsn: 2.027 ± 0.023	1.751PhePro: 1.751 ± 0.022	1.526PheGln: 1.526 ± 0.02	1.957PheArg: 1.957 ± 0.021	3.334PheSer: 3.334 ± 0.03	2.399PheThr: 2.399 ± 0.025	2.482PheVal: 2.482 ± 0.023	0.491PheTrp: 0.491 ± 0.011	1.503PheTyr: 1.503 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	3.144GlyAla: 3.144 ± 0.036	1.083GlyCys: 1.083 ± 0.019	2.918GlyAsp: 2.918 ± 0.035	3.426GlyGlu: 3.426 ± 0.034	2.331GlyPhe: 2.331 ± 0.025	4.551GlyGly: 4.551 ± 0.072	1.483GlyHis: 1.483 ± 0.029	3.358GlyIle: 3.358 ± 0.029	3.677GlyLys: 3.677 ± 0.029	4.847GlyLeu: 4.847 ± 0.038	1.259GlyMet: 1.259 ± 0.018	2.668GlyAsn: 2.668 ± 0.027	2.306GlyPro: 2.306 ± 0.042	2.055GlyGln: 2.055 ± 0.035	2.951GlyArg: 2.951 ± 0.029	4.397GlySer: 4.397 ± 0.042	3.174GlyThr: 3.174 ± 0.03	3.562GlyVal: 3.562 ± 0.031	0.7GlyTrp: 0.7 ± 0.013	2.057GlyTyr: 2.057 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	1.176HisAla: 1.176 ± 0.016	0.605HisCys: 0.605 ± 0.013	1.024HisAsp: 1.024 ± 0.015	1.237HisGlu: 1.237 ± 0.017	1.133HisPhe: 1.133 ± 0.018	1.31HisGly: 1.31 ± 0.025	1.051HisHis: 1.051 ± 0.023	1.493HisIle: 1.493 ± 0.018	1.45HisLys: 1.45 ± 0.017	2.541HisLeu: 2.541 ± 0.023	0.588HisMet: 0.588 ± 0.014	1.147HisAsn: 1.147 ± 0.015	1.314HisPro: 1.314 ± 0.017	1.049HisGln: 1.049 ± 0.017	1.297HisArg: 1.297 ± 0.018	1.894HisSer: 1.894 ± 0.025	1.272HisThr: 1.272 ± 0.02	1.385HisVal: 1.385 ± 0.018	0.312HisTrp: 0.312 ± 0.009	0.976HisTyr: 0.976 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	3.598IleAla: 3.598 ± 0.03	1.324IleCys: 1.324 ± 0.021	3.101IleAsp: 3.101 ± 0.025	3.717IleGlu: 3.717 ± 0.029	2.593IlePhe: 2.593 ± 0.026	3.1IleGly: 3.1 ± 0.027	1.399IleHis: 1.399 ± 0.019	3.94IleIle: 3.94 ± 0.035	4.205IleLys: 4.205 ± 0.034	5.78IleLeu: 5.78 ± 0.048	1.38IleMet: 1.38 ± 0.02	3.174IleAsn: 3.174 ± 0.026	2.883IlePro: 2.883 ± 0.024	2.205IleGln: 2.205 ± 0.021	2.844IleArg: 2.844 ± 0.024	4.9IleSer: 4.9 ± 0.037	3.574IleThr: 3.574 ± 0.035	3.841IleVal: 3.841 ± 0.029	0.653IleTrp: 0.653 ± 0.011	1.981IleTyr: 1.981 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	3.623LysAla: 3.623 ± 0.033	1.442LysCys: 1.442 ± 0.024	3.499LysAsp: 3.499 ± 0.032	5.605LysGlu: 5.605 ± 0.05	2.519LysPhe: 2.519 ± 0.023	3.226LysGly: 3.226 ± 0.037	1.585LysHis: 1.585 ± 0.02	4.189LysIle: 4.189 ± 0.033	5.948LysLys: 5.948 ± 0.066	6.598LysLeu: 6.598 ± 0.046	1.715LysMet: 1.715 ± 0.017	3.522LysAsn: 3.522 ± 0.036	2.967LysPro: 2.967 ± 0.033	2.75LysGln: 2.75 ± 0.026	3.745LysArg: 3.745 ± 0.035	4.721LysSer: 4.721 ± 0.037	3.683LysThr: 3.683 ± 0.029	4.163LysVal: 4.163 ± 0.034	0.774LysTrp: 0.774 ± 0.014	2.412LysTyr: 2.412 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	6.115LeuAla: 6.115 ± 0.038	1.972LeuCys: 1.972 ± 0.021	4.881LeuAsp: 4.881 ± 0.036	6.361LeuGlu: 6.361 ± 0.051	3.836LeuPhe: 3.836 ± 0.034	4.854LeuGly: 4.854 ± 0.039	2.534LeuHis: 2.534 ± 0.027	5.489LeuIle: 5.489 ± 0.041	6.908LeuLys: 6.908 ± 0.045	9.784LeuLeu: 9.784 ± 0.064	2.221LeuMet: 2.221 ± 0.024	4.74LeuAsn: 4.74 ± 0.036	4.807LeuPro: 4.807 ± 0.04	4.334LeuGln: 4.334 ± 0.034	5.107LeuArg: 5.107 ± 0.035	7.454LeuSer: 7.454 ± 0.05	5.33LeuThr: 5.33 ± 0.034	5.696LeuVal: 5.696 ± 0.039	1.052LeuTrp: 1.052 ± 0.012	3.082LeuTyr: 3.082 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	1.683MetAla: 1.683 ± 0.019	0.452MetCys: 0.452 ± 0.011	1.309MetAsp: 1.309 ± 0.016	1.643MetGlu: 1.643 ± 0.017	0.944MetPhe: 0.944 ± 0.012	1.177MetGly: 1.177 ± 0.016	0.441MetHis: 0.441 ± 0.009	1.202MetIle: 1.202 ± 0.017	1.707MetLys: 1.707 ± 0.023	2.098MetLeu: 2.098 ± 0.025	0.592MetMet: 0.592 ± 0.011	1.099MetAsn: 1.099 ± 0.013	1.022MetPro: 1.022 ± 0.019	0.845MetGln: 0.845 ± 0.013	1.103MetArg: 1.103 ± 0.016	1.781MetSer: 1.781 ± 0.016	1.232MetThr: 1.232 ± 0.016	1.455MetVal: 1.455 ± 0.018	0.266MetTrp: 0.266 ± 0.007	0.765MetTyr: 0.765 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.567AsnAla: 2.567 ± 0.027	1.096AsnCys: 1.096 ± 0.017	2.459AsnAsp: 2.459 ± 0.027	3.285AsnGlu: 3.285 ± 0.034	2.079AsnPhe: 2.079 ± 0.022	2.806AsnGly: 2.806 ± 0.033	1.103AsnHis: 1.103 ± 0.016	3.48AsnIle: 3.48 ± 0.03	3.557AsnLys: 3.557 ± 0.032	4.882AsnLeu: 4.882 ± 0.042	1.155AsnMet: 1.155 ± 0.015	3.09AsnAsn: 3.09 ± 0.035	2.13AsnPro: 2.13 ± 0.023	1.833AsnGln: 1.833 ± 0.025	2.267AsnArg: 2.267 ± 0.027	3.974AsnSer: 3.974 ± 0.033	2.641AsnThr: 2.641 ± 0.025	3.247AsnVal: 3.247 ± 0.03	0.572AsnTrp: 0.572 ± 0.012	1.834AsnTyr: 1.834 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	2.814ProAla: 2.814 ± 0.032	0.816ProCys: 0.816 ± 0.021	2.379ProAsp: 2.379 ± 0.025	3.213ProGlu: 3.213 ± 0.041	1.828ProPhe: 1.828 ± 0.023	2.723ProGly: 2.723 ± 0.071	1.157ProHis: 1.157 ± 0.016	2.506ProIle: 2.506 ± 0.026	2.922ProLys: 2.922 ± 0.03	4.256ProLeu: 4.256 ± 0.035	0.863ProMet: 0.863 ± 0.016	2.179ProAsn: 2.179 ± 0.026	4.021ProPro: 4.021 ± 0.066	2.043ProGln: 2.043 ± 0.027	2.1ProArg: 2.1 ± 0.024	4.102ProSer: 4.102 ± 0.052	2.882ProThr: 2.882 ± 0.03	3.348ProVal: 3.348 ± 0.043	0.518ProTrp: 0.518 ± 0.01	1.613ProTyr: 1.613 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	2.314GlnAla: 2.314 ± 0.025	0.799GlnCys: 0.799 ± 0.016	1.747GlnAsp: 1.747 ± 0.022	2.759GlnGlu: 2.759 ± 0.031	1.525GlnPhe: 1.525 ± 0.015	1.887GlnGly: 1.887 ± 0.021	1.076GlnHis: 1.076 ± 0.017	2.334GlnIle: 2.334 ± 0.02	2.47GlnLys: 2.47 ± 0.024	3.978GlnLeu: 3.978 ± 0.034	0.973GlnMet: 0.973 ± 0.015	1.933GlnAsn: 1.933 ± 0.023	2.021GlnPro: 2.021 ± 0.045	2.677GlnGln: 2.677 ± 0.061	2.047GlnArg: 2.047 ± 0.025	2.676GlnSer: 2.676 ± 0.03	2.088GlnThr: 2.088 ± 0.026	2.488GlnVal: 2.488 ± 0.026	0.447GlnTrp: 0.447 ± 0.01	1.264GlnTyr: 1.264 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	2.788ArgAla: 2.788 ± 0.026	0.996ArgCys: 0.996 ± 0.021	2.507ArgAsp: 2.507 ± 0.024	3.334ArgGlu: 3.334 ± 0.033	1.907ArgPhe: 1.907 ± 0.023	2.676ArgGly: 2.676 ± 0.034	1.322ArgHis: 1.322 ± 0.019	2.946ArgIle: 2.946 ± 0.025	3.954ArgLys: 3.954 ± 0.039	4.862ArgLeu: 4.862 ± 0.033	1.186ArgMet: 1.186 ± 0.014	2.596ArgAsn: 2.596 ± 0.027	2.365ArgPro: 2.365 ± 0.046	2.008ArgGln: 2.008 ± 0.024	3.543ArgArg: 3.543 ± 0.038	3.679ArgSer: 3.679 ± 0.039	2.751ArgThr: 2.751 ± 0.033	2.852ArgVal: 2.852 ± 0.027	0.606ArgTrp: 0.606 ± 0.01	1.691ArgTyr: 1.691 ± 0.024	0.0ArgXaa: 0.0 ± 0.0
Ser	4.579SerAla: 4.579 ± 0.037	1.585SerCys: 1.585 ± 0.022	4.117SerAsp: 4.117 ± 0.032	4.886SerGlu: 4.886 ± 0.043	3.045SerPhe: 3.045 ± 0.025	4.846SerGly: 4.846 ± 0.049	1.75SerHis: 1.75 ± 0.022	4.108SerIle: 4.108 ± 0.031	4.814SerLys: 4.814 ± 0.038	7.608SerLeu: 7.608 ± 0.046	1.552SerMet: 1.552 ± 0.018	3.798SerAsn: 3.798 ± 0.034	4.089SerPro: 4.089 ± 0.049	2.976SerGln: 2.976 ± 0.03	3.778SerArg: 3.778 ± 0.038	7.842SerSer: 7.842 ± 0.08	4.736SerThr: 4.736 ± 0.042	4.978SerVal: 4.978 ± 0.038	0.869SerTrp: 0.869 ± 0.014	2.43SerTyr: 2.43 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	3.535ThrAla: 3.535 ± 0.029	1.191ThrCys: 1.191 ± 0.022	2.785ThrAsp: 2.785 ± 0.026	3.478ThrGlu: 3.478 ± 0.033	2.335ThrPhe: 2.335 ± 0.024	3.298ThrGly: 3.298 ± 0.034	1.227ThrHis: 1.227 ± 0.019	3.336ThrIle: 3.336 ± 0.028	3.556ThrLys: 3.556 ± 0.038	5.568ThrLeu: 5.568 ± 0.038	1.187ThrMet: 1.187 ± 0.015	2.766ThrAsn: 2.766 ± 0.024	3.152ThrPro: 3.152 ± 0.035	1.913ThrGln: 1.913 ± 0.022	2.418ThrArg: 2.418 ± 0.023	4.976ThrSer: 4.976 ± 0.047	3.852ThrThr: 3.852 ± 0.056	3.975ThrVal: 3.975 ± 0.037	0.597ThrTrp: 0.597 ± 0.012	1.759ThrTyr: 1.759 ± 0.021	0.0ThrXaa: 0.0 ± 0.0
Val	4.118ValAla: 4.118 ± 0.039	1.429ValCys: 1.429 ± 0.021	3.35ValAsp: 3.35 ± 0.028	4.138ValGlu: 4.138 ± 0.038	2.54ValPhe: 2.54 ± 0.027	3.411ValGly: 3.411 ± 0.029	1.567ValHis: 1.567 ± 0.022	3.863ValIle: 3.863 ± 0.031	4.236ValLys: 4.236 ± 0.036	5.984ValLeu: 5.984 ± 0.039	1.467ValMet: 1.467 ± 0.015	3.059ValAsn: 3.059 ± 0.028	3.163ValPro: 3.163 ± 0.036	2.406ValGln: 2.406 ± 0.028	3.061ValArg: 3.061 ± 0.027	4.78ValSer: 4.78 ± 0.036	3.948ValThr: 3.948 ± 0.033	4.291ValVal: 4.291 ± 0.035	0.749ValTrp: 0.749 ± 0.014	2.074ValTyr: 2.074 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	0.658TrpAla: 0.658 ± 0.013	0.235TrpCys: 0.235 ± 0.008	0.635TrpAsp: 0.635 ± 0.012	0.666TrpGlu: 0.666 ± 0.011	0.506TrpPhe: 0.506 ± 0.01	0.589TrpGly: 0.589 ± 0.012	0.278TrpHis: 0.278 ± 0.009	0.712TrpIle: 0.712 ± 0.013	0.828TrpLys: 0.828 ± 0.015	1.215TrpLeu: 1.215 ± 0.019	0.286TrpMet: 0.286 ± 0.008	0.63TrpAsn: 0.63 ± 0.013	0.443TrpPro: 0.443 ± 0.01	0.438TrpGln: 0.438 ± 0.01	0.673TrpArg: 0.673 ± 0.015	0.828TrpSer: 0.828 ± 0.014	0.635TrpThr: 0.635 ± 0.01	0.664TrpVal: 0.664 ± 0.013	0.182TrpTrp: 0.182 ± 0.007	0.403TrpTyr: 0.403 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.69TyrAla: 1.69 ± 0.02	0.813TyrCys: 0.813 ± 0.014	1.736TyrAsp: 1.736 ± 0.02	1.871TyrGlu: 1.871 ± 0.022	1.702TyrPhe: 1.702 ± 0.023	1.979TyrGly: 1.979 ± 0.029	0.865TyrHis: 0.865 ± 0.014	2.02TyrIle: 2.02 ± 0.022	2.171TyrLys: 2.171 ± 0.02	3.37TyrLeu: 3.37 ± 0.031	0.736TyrMet: 0.736 ± 0.012	1.8TyrAsn: 1.8 ± 0.023	1.497TyrPro: 1.497 ± 0.03	1.261TyrGln: 1.261 ± 0.022	1.759TyrArg: 1.759 ± 0.02	2.617TyrSer: 2.617 ± 0.028	1.857TyrThr: 1.857 ± 0.021	1.923TyrVal: 1.923 ± 0.019	0.436TyrTrp: 0.436 ± 0.009	1.341TyrTyr: 1.341 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 14941 proteins (5337290 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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