Shewanella phage SFCi1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0Z1H5|A0A1B0Z1H5_9CAUD Uncharacterized protein OS=Shewanella phage SFCi1 OX=1852599 PE=4 SV=1
MM1 pKa = 7.52IFKK4 pKa = 10.67LSDD7 pKa = 2.75ISAVLGALLAPSGGSLPSDD26 pKa = 4.02NITGGWATYY35 pKa = 10.52FNGDD39 pKa = 3.46QTPINIPAGVEE50 pKa = 4.05TKK52 pKa = 9.68LTLDD56 pKa = 3.66ASDD59 pKa = 4.36PNNVLDD65 pKa = 4.52EE66 pKa = 4.05YY67 pKa = 11.32LPLGVSSIWDD77 pKa = 3.5SANSQFDD84 pKa = 3.75FSQLSVGDD92 pKa = 3.85MVDD95 pKa = 2.89IRR97 pKa = 11.84VDD99 pKa = 3.44GTLTNSGFNEE109 pKa = 4.19SFNLNMVAAIGSAGEE124 pKa = 3.94FTLPFASGNRR134 pKa = 11.84LFAGTSDD141 pKa = 3.11VSRR144 pKa = 11.84YY145 pKa = 8.38NGVYY149 pKa = 9.89IGSQDD154 pKa = 3.84MIDD157 pKa = 3.96NPAEE161 pKa = 3.92LRR163 pKa = 11.84IVTTDD168 pKa = 3.08AASGFLIDD176 pKa = 5.13IYY178 pKa = 11.62VKK180 pKa = 10.17VLKK183 pKa = 10.9VGG185 pKa = 3.39

Molecular weight: 19.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0Z2V3|A0A1B0Z2V3_9CAUD Site-specific DNA-methyltransferase (cytosine-N(4)-specific) OS=Shewanella phage SFCi1 OX=1852599 PE=3 SV=1
MM1 pKa = 7.26RR2 pKa = 11.84TLVLKK7 pKa = 10.46SAQTTEE13 pKa = 3.97GTEE16 pKa = 4.07MIEE19 pKa = 3.97MNTRR23 pKa = 11.84DD24 pKa = 3.97LRR26 pKa = 11.84RR27 pKa = 11.84LEE29 pKa = 4.06EE30 pKa = 3.93NLRR33 pKa = 11.84DD34 pKa = 3.74LNAKK38 pKa = 9.53GIHH41 pKa = 4.94FAEE44 pKa = 4.44RR45 pKa = 11.84NTINDD50 pKa = 3.37MAFGTMHH57 pKa = 7.62EE58 pKa = 4.06ARR60 pKa = 11.84KK61 pKa = 8.26TITEE65 pKa = 3.88QFVNRR70 pKa = 11.84NKK72 pKa = 8.52WTMRR76 pKa = 11.84SVQVDD81 pKa = 3.71KK82 pKa = 11.21ARR84 pKa = 11.84KK85 pKa = 8.85LSDD88 pKa = 3.31SAEE91 pKa = 4.13VGSTEE96 pKa = 6.05DD97 pKa = 3.42YY98 pKa = 10.59MADD101 pKa = 3.41QEE103 pKa = 4.4FGRR106 pKa = 11.84IKK108 pKa = 10.14HH109 pKa = 6.01EE110 pKa = 4.18NMHH113 pKa = 6.39IPTPSASGEE122 pKa = 4.1SPRR125 pKa = 11.84ASVRR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84PVRR134 pKa = 11.84RR135 pKa = 11.84PNRR138 pKa = 11.84MSAITLKK145 pKa = 9.86RR146 pKa = 11.84AKK148 pKa = 9.96YY149 pKa = 10.5SGMTRR154 pKa = 11.84QQQNIATVKK163 pKa = 8.65EE164 pKa = 4.23AKK166 pKa = 10.44AEE168 pKa = 3.91GRR170 pKa = 11.84KK171 pKa = 9.0FVFLQRR177 pKa = 11.84GKK179 pKa = 10.49EE180 pKa = 3.7RR181 pKa = 11.84GIYY184 pKa = 9.88KK185 pKa = 10.12VFGTKK190 pKa = 10.09RR191 pKa = 11.84KK192 pKa = 9.54PKK194 pKa = 6.31TTKK197 pKa = 10.09IQDD200 pKa = 3.33LSRR203 pKa = 11.84RR204 pKa = 11.84VAEE207 pKa = 4.39VPRR210 pKa = 11.84NPWLAPSSATVMRR223 pKa = 11.84TAPQLYY229 pKa = 7.94ATRR232 pKa = 11.84LQQQLDD238 pKa = 4.08RR239 pKa = 11.84LRR241 pKa = 11.84MRR243 pKa = 4.66

Molecular weight: 28.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29

0

29

10493

105

1100

361.8

39.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.893 ± 0.55

1.258 ± 0.26

6.452 ± 0.211

5.432 ± 0.311

3.545 ± 0.178

8.482 ± 0.424

1.334 ± 0.225

4.422 ± 0.268

4.184 ± 0.266

8.072 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.192

4.193 ± 0.348

4.736 ± 0.328

4.203 ± 0.28

5.594 ± 0.377

6.118 ± 0.256

7.014 ± 0.425

7.405 ± 0.315

1.534 ± 0.185

2.621 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski