Amino acid dipepetide frequency for Shewanella phage SFCi1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.961AlaAla: 10.961 ± 1.502
1.716AlaCys: 1.716 ± 0.501
6.958AlaAsp: 6.958 ± 0.888
6.672AlaGlu: 6.672 ± 0.773
3.241AlaPhe: 3.241 ± 0.609
9.245AlaGly: 9.245 ± 1.054
0.953AlaHis: 0.953 ± 0.38
5.528AlaIle: 5.528 ± 0.716
4.861AlaLys: 4.861 ± 0.889
8.101AlaLeu: 8.101 ± 0.953
2.669AlaMet: 2.669 ± 0.533
4.956AlaAsn: 4.956 ± 0.696
4.48AlaPro: 4.48 ± 0.471
5.433AlaGln: 5.433 ± 0.973
4.956AlaArg: 4.956 ± 0.598
7.148AlaSer: 7.148 ± 0.735
8.292AlaThr: 8.292 ± 1.293
7.911AlaVal: 7.911 ± 0.764
1.716AlaTrp: 1.716 ± 0.547
3.145AlaTyr: 3.145 ± 0.529
0.0AlaXaa: 0.0 ± 0.0
Cys
1.334CysAla: 1.334 ± 0.449
0.286CysCys: 0.286 ± 0.15
0.667CysAsp: 0.667 ± 0.239
1.048CysGlu: 1.048 ± 0.352
0.286CysPhe: 0.286 ± 0.134
1.811CysGly: 1.811 ± 0.635
0.286CysHis: 0.286 ± 0.137
0.381CysIle: 0.381 ± 0.183
0.0CysLys: 0.0 ± 0.0
1.144CysLeu: 1.144 ± 0.302
0.286CysMet: 0.286 ± 0.161
0.286CysAsn: 0.286 ± 0.156
0.858CysPro: 0.858 ± 0.322
0.477CysGln: 0.477 ± 0.182
0.667CysArg: 0.667 ± 0.289
1.048CysSer: 1.048 ± 0.35
0.572CysThr: 0.572 ± 0.235
0.953CysVal: 0.953 ± 0.38
0.095CysTrp: 0.095 ± 0.105
0.095CysTyr: 0.095 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
6.672AspAla: 6.672 ± 0.741
0.762AspCys: 0.762 ± 0.242
3.241AspAsp: 3.241 ± 0.576
4.289AspGlu: 4.289 ± 0.716
2.383AspPhe: 2.383 ± 0.348
6.767AspGly: 6.767 ± 0.739
0.572AspHis: 0.572 ± 0.253
3.526AspIle: 3.526 ± 0.631
2.383AspLys: 2.383 ± 0.386
6.291AspLeu: 6.291 ± 0.674
2.192AspMet: 2.192 ± 0.422
2.192AspAsn: 2.192 ± 0.444
3.622AspPro: 3.622 ± 0.696
2.097AspGln: 2.097 ± 0.421
2.573AspArg: 2.573 ± 0.498
3.526AspSer: 3.526 ± 0.435
4.098AspThr: 4.098 ± 0.722
5.337AspVal: 5.337 ± 0.552
0.381AspTrp: 0.381 ± 0.156
1.62AspTyr: 1.62 ± 0.294
0.0AspXaa: 0.0 ± 0.0
Glu
5.909GluAla: 5.909 ± 0.752
0.572GluCys: 0.572 ± 0.241
3.05GluAsp: 3.05 ± 0.481
1.716GluGlu: 1.716 ± 0.438
2.764GluPhe: 2.764 ± 0.464
3.812GluGly: 3.812 ± 0.633
0.858GluHis: 0.858 ± 0.263
2.478GluIle: 2.478 ± 0.49
2.002GluLys: 2.002 ± 0.433
5.147GluLeu: 5.147 ± 0.995
1.43GluMet: 1.43 ± 0.478
1.811GluAsn: 1.811 ± 0.482
2.002GluPro: 2.002 ± 0.666
2.478GluGln: 2.478 ± 0.408
3.622GluArg: 3.622 ± 0.763
2.859GluSer: 2.859 ± 0.471
3.526GluThr: 3.526 ± 0.539
5.051GluVal: 5.051 ± 0.75
1.525GluTrp: 1.525 ± 0.381
1.716GluTyr: 1.716 ± 0.297
0.0GluXaa: 0.0 ± 0.0
Phe
2.669PheAla: 2.669 ± 0.433
0.858PheCys: 0.858 ± 0.288
2.383PheAsp: 2.383 ± 0.526
2.764PheGlu: 2.764 ± 0.469
1.43PhePhe: 1.43 ± 0.262
4.384PheGly: 4.384 ± 0.753
0.191PheHis: 0.191 ± 0.113
1.334PheIle: 1.334 ± 0.335
1.811PheLys: 1.811 ± 0.345
2.383PheLeu: 2.383 ± 0.6
1.334PheMet: 1.334 ± 0.36
2.573PheAsn: 2.573 ± 0.464
0.953PhePro: 0.953 ± 0.358
1.334PheGln: 1.334 ± 0.32
1.716PheArg: 1.716 ± 0.491
1.62PheSer: 1.62 ± 0.423
2.287PheThr: 2.287 ± 0.665
2.097PheVal: 2.097 ± 0.524
0.572PheTrp: 0.572 ± 0.186
0.762PheTyr: 0.762 ± 0.188
0.0PheXaa: 0.0 ± 0.0
Gly
8.959GlyAla: 8.959 ± 0.905
0.858GlyCys: 0.858 ± 0.339
5.147GlyAsp: 5.147 ± 0.712
4.766GlyGlu: 4.766 ± 0.59
3.145GlyPhe: 3.145 ± 0.495
7.434GlyGly: 7.434 ± 0.826
1.525GlyHis: 1.525 ± 0.353
3.145GlyIle: 3.145 ± 0.661
4.384GlyLys: 4.384 ± 0.705
7.53GlyLeu: 7.53 ± 0.807
1.43GlyMet: 1.43 ± 0.394
2.764GlyAsn: 2.764 ± 0.727
2.669GlyPro: 2.669 ± 0.554
3.05GlyGln: 3.05 ± 0.499
5.051GlyArg: 5.051 ± 0.6
4.575GlySer: 4.575 ± 0.756
6.481GlyThr: 6.481 ± 0.611
7.911GlyVal: 7.911 ± 0.965
1.811GlyTrp: 1.811 ± 0.612
2.192GlyTyr: 2.192 ± 0.606
0.0GlyXaa: 0.0 ± 0.0
His
0.858HisAla: 0.858 ± 0.301
0.191HisCys: 0.191 ± 0.157
0.858HisAsp: 0.858 ± 0.368
1.239HisGlu: 1.239 ± 0.324
0.286HisPhe: 0.286 ± 0.155
1.144HisGly: 1.144 ± 0.357
0.286HisHis: 0.286 ± 0.143
0.858HisIle: 0.858 ± 0.249
0.858HisLys: 0.858 ± 0.335
1.43HisLeu: 1.43 ± 0.378
0.381HisMet: 0.381 ± 0.209
0.667HisAsn: 0.667 ± 0.263
0.858HisPro: 0.858 ± 0.406
0.477HisGln: 0.477 ± 0.202
0.572HisArg: 0.572 ± 0.305
0.286HisSer: 0.286 ± 0.186
0.667HisThr: 0.667 ± 0.221
0.762HisVal: 0.762 ± 0.274
0.191HisTrp: 0.191 ± 0.138
0.477HisTyr: 0.477 ± 0.291
0.0HisXaa: 0.0 ± 0.0
Ile
4.003IleAla: 4.003 ± 0.577
0.477IleCys: 0.477 ± 0.236
4.289IleAsp: 4.289 ± 0.625
3.431IleGlu: 3.431 ± 0.419
1.43IlePhe: 1.43 ± 0.324
3.241IleGly: 3.241 ± 0.491
0.191IleHis: 0.191 ± 0.13
2.192IleIle: 2.192 ± 0.526
2.097IleLys: 2.097 ± 0.445
2.097IleLeu: 2.097 ± 0.356
1.239IleMet: 1.239 ± 0.396
2.669IleAsn: 2.669 ± 0.558
1.716IlePro: 1.716 ± 0.418
2.097IleGln: 2.097 ± 0.391
2.383IleArg: 2.383 ± 0.366
2.859IleSer: 2.859 ± 0.552
3.241IleThr: 3.241 ± 0.461
3.145IleVal: 3.145 ± 0.559
0.381IleTrp: 0.381 ± 0.173
0.953IleTyr: 0.953 ± 0.26
0.0IleXaa: 0.0 ± 0.0
Lys
5.051LysAla: 5.051 ± 0.608
0.286LysCys: 0.286 ± 0.134
2.955LysAsp: 2.955 ± 0.589
1.811LysGlu: 1.811 ± 0.42
1.62LysPhe: 1.62 ± 0.355
3.812LysGly: 3.812 ± 0.964
0.953LysHis: 0.953 ± 0.267
1.906LysIle: 1.906 ± 0.398
1.716LysLys: 1.716 ± 0.37
3.717LysLeu: 3.717 ± 0.788
0.667LysMet: 0.667 ± 0.233
1.62LysAsn: 1.62 ± 0.491
2.573LysPro: 2.573 ± 0.591
1.43LysGln: 1.43 ± 0.334
2.287LysArg: 2.287 ± 0.472
2.002LysSer: 2.002 ± 0.484
2.573LysThr: 2.573 ± 0.594
2.478LysVal: 2.478 ± 0.51
1.048LysTrp: 1.048 ± 0.247
1.334LysTyr: 1.334 ± 0.321
0.0LysXaa: 0.0 ± 0.0
Leu
10.579LeuAla: 10.579 ± 1.198
0.667LeuCys: 0.667 ± 0.248
6.767LeuAsp: 6.767 ± 0.988
3.717LeuGlu: 3.717 ± 0.592
2.478LeuPhe: 2.478 ± 0.404
4.766LeuGly: 4.766 ± 0.563
0.572LeuHis: 0.572 ± 0.319
3.241LeuIle: 3.241 ± 0.722
3.145LeuLys: 3.145 ± 0.578
4.956LeuLeu: 4.956 ± 0.681
1.144LeuMet: 1.144 ± 0.307
3.526LeuAsn: 3.526 ± 0.536
4.575LeuPro: 4.575 ± 0.579
3.526LeuGln: 3.526 ± 0.719
5.528LeuArg: 5.528 ± 0.795
6.195LeuSer: 6.195 ± 0.437
6.291LeuThr: 6.291 ± 0.794
6.576LeuVal: 6.576 ± 0.848
0.667LeuTrp: 0.667 ± 0.173
1.811LeuTyr: 1.811 ± 0.43
0.0LeuXaa: 0.0 ± 0.0
Met
3.336MetAla: 3.336 ± 0.628
0.572MetCys: 0.572 ± 0.234
0.953MetAsp: 0.953 ± 0.255
0.477MetGlu: 0.477 ± 0.229
1.62MetPhe: 1.62 ± 0.321
1.048MetGly: 1.048 ± 0.378
0.667MetHis: 0.667 ± 0.265
1.334MetIle: 1.334 ± 0.349
0.858MetLys: 0.858 ± 0.298
1.62MetLeu: 1.62 ± 0.373
0.572MetMet: 0.572 ± 0.202
0.381MetAsn: 0.381 ± 0.195
1.62MetPro: 1.62 ± 0.337
1.144MetGln: 1.144 ± 0.376
1.62MetArg: 1.62 ± 0.499
2.955MetSer: 2.955 ± 0.415
2.002MetThr: 2.002 ± 0.354
1.62MetVal: 1.62 ± 0.359
0.191MetTrp: 0.191 ± 0.131
0.477MetTyr: 0.477 ± 0.193
0.0MetXaa: 0.0 ± 0.0
Asn
5.433AsnAla: 5.433 ± 0.614
0.572AsnCys: 0.572 ± 0.224
2.764AsnAsp: 2.764 ± 0.464
1.43AsnGlu: 1.43 ± 0.287
1.144AsnPhe: 1.144 ± 0.277
4.861AsnGly: 4.861 ± 0.963
0.381AsnHis: 0.381 ± 0.182
1.811AsnIle: 1.811 ± 0.439
1.525AsnLys: 1.525 ± 0.424
3.431AsnLeu: 3.431 ± 0.516
1.239AsnMet: 1.239 ± 0.329
2.097AsnAsn: 2.097 ± 0.457
2.573AsnPro: 2.573 ± 0.433
1.239AsnGln: 1.239 ± 0.309
2.287AsnArg: 2.287 ± 0.359
2.287AsnSer: 2.287 ± 0.52
3.336AsnThr: 3.336 ± 0.504
2.573AsnVal: 2.573 ± 0.443
0.381AsnTrp: 0.381 ± 0.184
0.572AsnTyr: 0.572 ± 0.222
0.0AsnXaa: 0.0 ± 0.0
Pro
4.861ProAla: 4.861 ± 0.719
0.762ProCys: 0.762 ± 0.249
3.622ProAsp: 3.622 ± 0.482
2.955ProGlu: 2.955 ± 0.803
2.192ProPhe: 2.192 ± 0.39
3.145ProGly: 3.145 ± 0.593
0.572ProHis: 0.572 ± 0.243
1.525ProIle: 1.525 ± 0.36
2.097ProLys: 2.097 ± 0.592
3.717ProLeu: 3.717 ± 0.643
1.144ProMet: 1.144 ± 0.349
2.287ProAsn: 2.287 ± 0.388
1.334ProPro: 1.334 ± 0.374
2.192ProGln: 2.192 ± 0.52
1.811ProArg: 1.811 ± 0.445
4.67ProSer: 4.67 ± 0.607
3.145ProThr: 3.145 ± 0.566
2.955ProVal: 2.955 ± 0.393
0.667ProTrp: 0.667 ± 0.24
1.716ProTyr: 1.716 ± 0.252
0.0ProXaa: 0.0 ± 0.0
Gln
4.384GlnAla: 4.384 ± 0.518
0.381GlnCys: 0.381 ± 0.21
1.906GlnAsp: 1.906 ± 0.439
1.525GlnGlu: 1.525 ± 0.311
1.334GlnPhe: 1.334 ± 0.353
3.336GlnGly: 3.336 ± 0.53
0.477GlnHis: 0.477 ± 0.178
2.573GlnIle: 2.573 ± 0.574
1.334GlnLys: 1.334 ± 0.308
4.098GlnLeu: 4.098 ± 0.558
1.239GlnMet: 1.239 ± 0.329
1.716GlnAsn: 1.716 ± 0.553
2.002GlnPro: 2.002 ± 0.354
2.287GlnGln: 2.287 ± 0.588
3.526GlnArg: 3.526 ± 0.466
2.002GlnSer: 2.002 ± 0.421
2.764GlnThr: 2.764 ± 0.453
3.622GlnVal: 3.622 ± 0.609
0.667GlnTrp: 0.667 ± 0.239
0.858GlnTyr: 0.858 ± 0.263
0.0GlnXaa: 0.0 ± 0.0
Arg
5.528ArgAla: 5.528 ± 0.798
0.381ArgCys: 0.381 ± 0.247
4.003ArgAsp: 4.003 ± 0.611
3.241ArgGlu: 3.241 ± 0.593
2.192ArgPhe: 2.192 ± 0.422
4.48ArgGly: 4.48 ± 0.753
0.858ArgHis: 0.858 ± 0.332
2.669ArgIle: 2.669 ± 0.42
2.764ArgLys: 2.764 ± 0.539
4.48ArgLeu: 4.48 ± 0.712
1.144ArgMet: 1.144 ± 0.383
1.811ArgAsn: 1.811 ± 0.363
3.145ArgPro: 3.145 ± 0.512
2.573ArgGln: 2.573 ± 0.459
4.194ArgArg: 4.194 ± 0.722
2.669ArgSer: 2.669 ± 0.403
3.145ArgThr: 3.145 ± 0.562
4.48ArgVal: 4.48 ± 0.516
0.762ArgTrp: 0.762 ± 0.229
1.43ArgTyr: 1.43 ± 0.448
0.0ArgXaa: 0.0 ± 0.0
Ser
8.959SerAla: 8.959 ± 1.021
0.572SerCys: 0.572 ± 0.201
4.289SerAsp: 4.289 ± 0.683
2.097SerGlu: 2.097 ± 0.42
2.002SerPhe: 2.002 ± 0.411
5.147SerGly: 5.147 ± 0.987
1.239SerHis: 1.239 ± 0.377
2.478SerIle: 2.478 ± 0.564
2.478SerLys: 2.478 ± 0.556
5.051SerLeu: 5.051 ± 0.654
1.525SerMet: 1.525 ± 0.485
2.097SerAsn: 2.097 ± 0.41
2.573SerPro: 2.573 ± 0.465
3.431SerGln: 3.431 ± 0.63
2.955SerArg: 2.955 ± 0.505
3.05SerSer: 3.05 ± 0.659
4.289SerThr: 4.289 ± 0.453
4.48SerVal: 4.48 ± 0.681
0.953SerTrp: 0.953 ± 0.259
1.525SerTyr: 1.525 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
7.434ThrAla: 7.434 ± 0.91
0.572ThrCys: 0.572 ± 0.213
4.098ThrAsp: 4.098 ± 0.576
3.717ThrGlu: 3.717 ± 0.623
3.241ThrPhe: 3.241 ± 0.486
7.148ThrGly: 7.148 ± 0.761
0.477ThrHis: 0.477 ± 0.279
2.764ThrIle: 2.764 ± 0.521
2.955ThrLys: 2.955 ± 0.4
5.051ThrLeu: 5.051 ± 0.724
2.573ThrMet: 2.573 ± 0.464
3.05ThrAsn: 3.05 ± 0.65
4.098ThrPro: 4.098 ± 0.686
2.764ThrGln: 2.764 ± 0.415
3.241ThrArg: 3.241 ± 0.572
3.717ThrSer: 3.717 ± 0.725
5.051ThrThr: 5.051 ± 1.168
5.337ThrVal: 5.337 ± 0.624
0.953ThrTrp: 0.953 ± 0.276
1.906ThrTyr: 1.906 ± 0.383
0.0ThrXaa: 0.0 ± 0.0
Val
8.197ValAla: 8.197 ± 1.145
1.239ValCys: 1.239 ± 0.296
4.384ValAsp: 4.384 ± 0.446
4.575ValGlu: 4.575 ± 0.536
1.811ValPhe: 1.811 ± 0.388
5.147ValGly: 5.147 ± 0.542
1.62ValHis: 1.62 ± 0.471
3.145ValIle: 3.145 ± 0.661
2.955ValLys: 2.955 ± 0.455
6.958ValLeu: 6.958 ± 0.639
1.811ValMet: 1.811 ± 0.338
3.526ValAsn: 3.526 ± 0.578
3.05ValPro: 3.05 ± 0.399
2.859ValGln: 2.859 ± 0.537
4.384ValArg: 4.384 ± 0.661
5.719ValSer: 5.719 ± 0.894
5.433ValThr: 5.433 ± 1.074
3.812ValVal: 3.812 ± 0.709
1.144ValTrp: 1.144 ± 0.283
2.287ValTyr: 2.287 ± 0.455
0.0ValXaa: 0.0 ± 0.0
Trp
1.716TrpAla: 1.716 ± 0.449
0.095TrpCys: 0.095 ± 0.08
1.048TrpAsp: 1.048 ± 0.459
0.762TrpGlu: 0.762 ± 0.292
0.095TrpPhe: 0.095 ± 0.09
1.334TrpGly: 1.334 ± 0.398
0.191TrpHis: 0.191 ± 0.133
0.286TrpIle: 0.286 ± 0.165
0.477TrpLys: 0.477 ± 0.162
1.811TrpLeu: 1.811 ± 0.487
0.286TrpMet: 0.286 ± 0.168
0.477TrpAsn: 0.477 ± 0.168
1.239TrpPro: 1.239 ± 0.371
0.191TrpGln: 0.191 ± 0.121
1.334TrpArg: 1.334 ± 0.254
0.953TrpSer: 0.953 ± 0.323
1.048TrpThr: 1.048 ± 0.247
1.048TrpVal: 1.048 ± 0.284
0.572TrpTrp: 0.572 ± 0.22
0.381TrpTyr: 0.381 ± 0.183
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.097TyrAla: 2.097 ± 0.353
0.762TyrCys: 0.762 ± 0.343
1.144TyrAsp: 1.144 ± 0.3
2.097TyrGlu: 2.097 ± 0.453
0.762TyrPhe: 0.762 ± 0.221
2.764TyrGly: 2.764 ± 0.498
0.477TyrHis: 0.477 ± 0.162
1.048TyrIle: 1.048 ± 0.497
1.144TyrLys: 1.144 ± 0.247
1.716TyrLeu: 1.716 ± 0.406
0.667TyrMet: 0.667 ± 0.254
1.43TyrAsn: 1.43 ± 0.357
1.43TyrPro: 1.43 ± 0.301
0.953TyrGln: 0.953 ± 0.229
1.239TyrArg: 1.239 ± 0.384
1.048TyrSer: 1.048 ± 0.25
1.906TyrThr: 1.906 ± 0.389
1.906TyrVal: 1.906 ± 0.47
0.667TyrTrp: 0.667 ± 0.283
0.953TyrTyr: 0.953 ± 0.466
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 29 proteins (10493 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski