Antonospora locustae virus 1
Average proteome isoelectric point is 7.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A218KIR8|A0A218KIR8_9VIRU ORF1/ORF2 fusion protein OS=Antonospora locustae virus 1 OX=1872708 PE=4 SV=1
MM1 pKa = 7.02 EE2 pKa = 5.45 ASVFVSRR9 pKa = 11.84 LMAIEE14 pKa = 4.58 PSDD17 pKa = 3.91 FSQHH21 pKa = 5.43 FSLPMSICDD30 pKa = 4.17 HH31 pKa = 5.8 GLKK34 pKa = 9.52 MIRR37 pKa = 11.84 ISAEE41 pKa = 3.46 QTPAFVKK48 pKa = 10.82 AFTTQYY54 pKa = 11.14 ALSNEE59 pKa = 4.33 CPQTEE64 pKa = 4.39 FFNALFDD71 pKa = 4.46 YY72 pKa = 11.23 IMDD75 pKa = 4.24 GKK77 pKa = 10.14 IKK79 pKa = 10.19 QAIGEE84 pKa = 4.03 ASGRR88 pKa = 11.84 LRR90 pKa = 11.84 KK91 pKa = 8.29 EE92 pKa = 3.63 QKK94 pKa = 10.26 LLQFQEE100 pKa = 3.89 LHH102 pKa = 6.28 GFSKK106 pKa = 10.75 DD107 pKa = 3.23 IEE109 pKa = 4.67 DD110 pKa = 4.91 DD111 pKa = 3.87 FNEE114 pKa = 4.99 ALILYY119 pKa = 8.83 NKK121 pKa = 9.02 EE122 pKa = 3.83 RR123 pKa = 11.84 ADD125 pKa = 3.69 LRR127 pKa = 11.84 SVKK130 pKa = 10.66 DD131 pKa = 3.66 KK132 pKa = 10.97 FDD134 pKa = 3.98 EE135 pKa = 4.26 LTKK138 pKa = 10.79 DD139 pKa = 3.16 LNGIFKK145 pKa = 10.63 VLEE148 pKa = 4.19 EE149 pKa = 4.4 EE150 pKa = 5.16 LSQRR154 pKa = 11.84 WSALEE159 pKa = 3.85 TIINEE164 pKa = 4.36 KK165 pKa = 8.33 KK166 pKa = 8.96 TNAAEE171 pKa = 3.95 AQKK174 pKa = 10.36 RR175 pKa = 11.84 ARR177 pKa = 11.84 TNWINMKK184 pKa = 10.12 QADD187 pKa = 4.33 RR188 pKa = 11.84 QKK190 pKa = 10.82 YY191 pKa = 9.71 SNDD194 pKa = 2.35 FMLYY198 pKa = 9.71 LKK200 pKa = 10.3 EE201 pKa = 3.68 YY202 pKa = 10.16 SLRR205 pKa = 11.84 NKK207 pKa = 10.11 VNFDD211 pKa = 3.32 QKK213 pKa = 11.11 LKK215 pKa = 10.8 SNEE218 pKa = 3.89 DD219 pKa = 3.23 FKK221 pKa = 11.77 KK222 pKa = 10.33 KK223 pKa = 10.48 LKK225 pKa = 10.18 EE226 pKa = 3.71 LLVKK230 pKa = 10.31 IRR232 pKa = 11.84 VEE234 pKa = 4.08 EE235 pKa = 4.01 NVDD238 pKa = 4.04 KK239 pKa = 11.15 IFLADD244 pKa = 3.59 GLINPKK250 pKa = 9.96 FKK252 pKa = 10.78 SEE254 pKa = 3.72 RR255 pKa = 11.84 VTFLVSEE262 pKa = 4.37 LFEE265 pKa = 4.32 KK266 pKa = 10.76 LGSIKK271 pKa = 10.49 KK272 pKa = 9.76 IFTSQRR278 pKa = 11.84 INRR281 pKa = 11.84 QSGVQEE287 pKa = 4.06 CTAGNLL293 pKa = 3.54
Molecular weight: 34.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.32
IPC2_protein 7.688
IPC_protein 7.673
Toseland 7.907
ProMoST 8.17
Dawson 8.507
Bjellqvist 8.536
Wikipedia 8.492
Rodwell 8.595
Grimsley 7.702
Solomon 8.712
Lehninger 8.712
Nozaki 8.785
DTASelect 8.419
Thurlkill 8.551
EMBOSS 8.741
Sillero 8.785
Patrickios 5.016
IPC_peptide 8.697
IPC2_peptide 7.22
IPC2.peptide.svr19 7.559
Protein with the highest isoelectric point:
>tr|A0A218KIR8|A0A218KIR8_9VIRU ORF1/ORF2 fusion protein OS=Antonospora locustae virus 1 OX=1872708 PE=4 SV=1
MM1 pKa = 7.02 EE2 pKa = 5.45 ASVFVSRR9 pKa = 11.84 LMAIEE14 pKa = 4.58 PSDD17 pKa = 3.91 FSQHH21 pKa = 5.43 FSLPMSICDD30 pKa = 4.17 HH31 pKa = 5.8 GLKK34 pKa = 9.52 MIRR37 pKa = 11.84 ISAEE41 pKa = 3.46 QTPAFVKK48 pKa = 10.82 AFTTQYY54 pKa = 11.14 ALSNEE59 pKa = 4.33 CPQTEE64 pKa = 4.39 FFNALFDD71 pKa = 4.46 YY72 pKa = 11.23 IMDD75 pKa = 4.24 GKK77 pKa = 10.14 IKK79 pKa = 10.19 QAIGEE84 pKa = 4.03 ASGRR88 pKa = 11.84 LRR90 pKa = 11.84 KK91 pKa = 8.29 EE92 pKa = 3.63 QKK94 pKa = 10.26 LLQFQEE100 pKa = 3.89 LHH102 pKa = 6.28 GFSKK106 pKa = 10.75 DD107 pKa = 3.23 IEE109 pKa = 4.67 DD110 pKa = 4.91 DD111 pKa = 3.87 FNEE114 pKa = 4.99 ALILYY119 pKa = 8.83 NKK121 pKa = 9.02 EE122 pKa = 3.83 RR123 pKa = 11.84 ADD125 pKa = 3.69 LRR127 pKa = 11.84 SVKK130 pKa = 10.66 DD131 pKa = 3.66 KK132 pKa = 10.97 FDD134 pKa = 3.98 EE135 pKa = 4.26 LTKK138 pKa = 10.79 DD139 pKa = 3.16 LNGIFKK145 pKa = 10.63 VLEE148 pKa = 4.19 EE149 pKa = 4.4 EE150 pKa = 5.16 LSQRR154 pKa = 11.84 WSALEE159 pKa = 3.85 TIINEE164 pKa = 4.36 KK165 pKa = 8.33 KK166 pKa = 8.96 TNAAEE171 pKa = 3.95 AQKK174 pKa = 10.36 RR175 pKa = 11.84 ARR177 pKa = 11.84 TNWINMKK184 pKa = 10.12 QADD187 pKa = 4.33 RR188 pKa = 11.84 QKK190 pKa = 10.82 YY191 pKa = 9.71 SNDD194 pKa = 2.35 FMLYY198 pKa = 9.71 LKK200 pKa = 10.3 EE201 pKa = 3.68 YY202 pKa = 10.16 SLRR205 pKa = 11.84 NKK207 pKa = 10.11 VNFDD211 pKa = 3.32 QKK213 pKa = 11.11 LKK215 pKa = 10.8 SNEE218 pKa = 3.89 DD219 pKa = 3.23 FKK221 pKa = 11.77 KK222 pKa = 10.33 KK223 pKa = 10.48 LKK225 pKa = 10.18 EE226 pKa = 3.71 LLVKK230 pKa = 10.31 IRR232 pKa = 11.84 VEE234 pKa = 4.08 EE235 pKa = 4.01 NVDD238 pKa = 4.04 KK239 pKa = 11.15 IFLADD244 pKa = 3.59 GLINPKK250 pKa = 9.96 FKK252 pKa = 10.78 SEE254 pKa = 3.77 RR255 pKa = 11.84 VTFLSQSYY263 pKa = 10.07 LRR265 pKa = 11.84 NLDD268 pKa = 3.26 RR269 pKa = 11.84 LRR271 pKa = 11.84 RR272 pKa = 11.84 SLQARR277 pKa = 11.84 GLTDD281 pKa = 3.19 SQEE284 pKa = 4.29 YY285 pKa = 10.95 KK286 pKa = 10.84 NVLQAIYY293 pKa = 10.83 DD294 pKa = 3.72 LLYY297 pKa = 11.0 NFNGPYY303 pKa = 9.96 LHH305 pKa = 7.65 PDD307 pKa = 3.55 FPGDD311 pKa = 3.41 FGHH314 pKa = 7.58 RR315 pKa = 11.84 SHH317 pKa = 7.07 ALANSFINPPLFSVPEE333 pKa = 3.87 QLDD336 pKa = 3.82 ANVFTSDD343 pKa = 3.35 CVSNGRR349 pKa = 11.84 YY350 pKa = 8.95 FKK352 pKa = 11.13 YY353 pKa = 10.89 NEE355 pKa = 4.03 FLRR358 pKa = 11.84 DD359 pKa = 3.41 SLGAIGGGANDD370 pKa = 4.44 LLRR373 pKa = 11.84 EE374 pKa = 3.95 LMEE377 pKa = 3.73 YY378 pKa = 10.31 RR379 pKa = 11.84 RR380 pKa = 11.84 LGGSTSDD387 pKa = 3.18 KK388 pKa = 10.97 LNYY391 pKa = 9.49 LAYY394 pKa = 10.37 FGNKK398 pKa = 7.6 SQCDD402 pKa = 3.6 TLLGLDD408 pKa = 3.69 TAPVYY413 pKa = 11.5 SMFVSGVVRR422 pKa = 11.84 EE423 pKa = 4.8 PYY425 pKa = 10.12 VHH427 pKa = 6.23 MPYY430 pKa = 10.55 SVLVKK435 pKa = 10.48 KK436 pKa = 8.42 FTYY439 pKa = 9.89 NAQASGGTLDD449 pKa = 3.88 KK450 pKa = 11.41 QFGFKK455 pKa = 10.42 NKK457 pKa = 10.22 SDD459 pKa = 3.78 VLLLNLVAYY468 pKa = 10.16 RR469 pKa = 11.84 LISTAWCIQKK479 pKa = 10.2 RR480 pKa = 11.84 SKK482 pKa = 10.58 LKK484 pKa = 10.49 PGIIWDD490 pKa = 3.68 SASRR494 pKa = 11.84 PKK496 pKa = 10.4 LIKK499 pKa = 10.14 IAGTGNKK506 pKa = 8.05 MKK508 pKa = 10.36 KK509 pKa = 9.78 IMAGEE514 pKa = 4.11 PCCRR518 pKa = 11.84 VIAVGPLLEE527 pKa = 4.42 SLLGYY532 pKa = 9.97 SLYY535 pKa = 10.91 LRR537 pKa = 11.84 VSEE540 pKa = 5.13 RR541 pKa = 11.84 LQQFFKK547 pKa = 10.89 EE548 pKa = 4.0 KK549 pKa = 10.31 GYY551 pKa = 11.13 GVAIGCNRR559 pKa = 11.84 MGKK562 pKa = 9.4 DD563 pKa = 2.85 WQNIQKK569 pKa = 9.33 NFKK572 pKa = 8.69 GARR575 pKa = 11.84 HH576 pKa = 5.72 IMVGDD581 pKa = 3.45 YY582 pKa = 10.92 SKK584 pKa = 11.52 YY585 pKa = 10.39 DD586 pKa = 3.18 QTIPEE591 pKa = 4.12 RR592 pKa = 11.84 LMIFGIDD599 pKa = 3.97 MILNMYY605 pKa = 8.49 MPQDD609 pKa = 4.34 EE610 pKa = 4.59 YY611 pKa = 11.84 SMNYY615 pKa = 9.02 IDD617 pKa = 6.17 NFRR620 pKa = 11.84 TWFIDD625 pKa = 3.59 NIVRR629 pKa = 11.84 SVHH632 pKa = 5.71 VVDD635 pKa = 5.28 GKK637 pKa = 11.11 LSARR641 pKa = 11.84 AIGGMPSGTLWTSLLNSVINIVVISDD667 pKa = 3.4 TCKK670 pKa = 10.45 ALGIKK675 pKa = 9.77 NYY677 pKa = 10.28 KK678 pKa = 8.44 PVVYY682 pKa = 10.11 GDD684 pKa = 3.45 DD685 pKa = 3.56 HH686 pKa = 6.69 MIIIYY691 pKa = 10.1 DD692 pKa = 3.99 EE693 pKa = 4.44 IVDD696 pKa = 3.68 SGEE699 pKa = 4.25 FISAYY704 pKa = 7.91 STYY707 pKa = 11.03 VKK709 pKa = 10.38 INFGMTLTTDD719 pKa = 3.35 DD720 pKa = 4.46 TYY722 pKa = 11.38 ISGPEE727 pKa = 4.09 CFYY730 pKa = 10.52 VTYY733 pKa = 10.27 KK734 pKa = 10.71 RR735 pKa = 11.84 PVYY738 pKa = 10.23 NPKK741 pKa = 10.51 ADD743 pKa = 3.71 LQKK746 pKa = 9.53 GTRR749 pKa = 11.84 RR750 pKa = 11.84 LRR752 pKa = 11.84 PKK754 pKa = 10.08 SWEE757 pKa = 3.87 TSKK760 pKa = 9.95 TPFTQFDD767 pKa = 3.68 HH768 pKa = 6.71 TKK770 pKa = 9.03 GTTHH774 pKa = 6.47 RR775 pKa = 11.84 WSYY778 pKa = 9.02 EE779 pKa = 3.25 FRR781 pKa = 11.84 GRR783 pKa = 11.84 PKK785 pKa = 10.21 FLQYY789 pKa = 11.01 YY790 pKa = 8.1 WLEE793 pKa = 3.97 TGLAIRR799 pKa = 11.84 PLRR802 pKa = 11.84 EE803 pKa = 3.69 SMVRR807 pKa = 11.84 MLHH810 pKa = 6.52 PEE812 pKa = 3.93 EE813 pKa = 4.01 EE814 pKa = 4.51 VKK816 pKa = 10.66 NVRR819 pKa = 11.84 EE820 pKa = 3.98 YY821 pKa = 9.65 EE822 pKa = 4.27 VLVISHH828 pKa = 7.32 LYY830 pKa = 10.34 DD831 pKa = 3.86 NYY833 pKa = 11.1 HH834 pKa = 5.99 NAHH837 pKa = 5.2 MRR839 pKa = 11.84 NWAFHH844 pKa = 6.59 LLYY847 pKa = 10.7 DD848 pKa = 3.53 IDD850 pKa = 3.89 FMKK853 pKa = 10.42 RR854 pKa = 11.84 ARR856 pKa = 11.84 MKK858 pKa = 10.79 SADD861 pKa = 3.84 LFFKK865 pKa = 10.8 RR866 pKa = 11.84 SDD868 pKa = 3.37 RR869 pKa = 11.84 GKK871 pKa = 10.92 GYY873 pKa = 10.51 YY874 pKa = 8.53 EE875 pKa = 4.3 HH876 pKa = 7.17 NKK878 pKa = 9.84 GPPSRR883 pKa = 11.84 MWYY886 pKa = 9.84 RR887 pKa = 11.84 RR888 pKa = 11.84 VDD890 pKa = 3.59 YY891 pKa = 10.93 LVDD894 pKa = 4.19 LDD896 pKa = 4.25 SCVGMQHH903 pKa = 6.54 FVHH906 pKa = 5.85 SWKK909 pKa = 10.75 VLLAQMDD916 pKa = 4.26 EE917 pKa = 4.75 IISSEE922 pKa = 4.34 HH923 pKa = 5.35 EE924 pKa = 4.03 CEE926 pKa = 4.37 PYY928 pKa = 10.0 QVRR931 pKa = 11.84 HH932 pKa = 6.36 LIKK935 pKa = 10.65 NEE937 pKa = 3.24 LSMRR941 pKa = 11.84 RR942 pKa = 11.84 ISTTRR947 pKa = 11.84 ARR949 pKa = 11.84 AWFEE953 pKa = 3.43 AGIITRR959 pKa = 11.84 SEE961 pKa = 4.06 YY962 pKa = 11.15 AQFLAAEE969 pKa = 4.64 GTSKK973 pKa = 10.42 QFEE976 pKa = 4.72 SKK978 pKa = 10.43 KK979 pKa = 10.8 SPFNLFGLDD988 pKa = 3.29 EE989 pKa = 4.37 VGFNYY994 pKa = 10.31 LFSLLGISNDD1004 pKa = 3.12 KK1005 pKa = 10.18 VHH1007 pKa = 6.68 YY1008 pKa = 9.25 STDD1011 pKa = 3.47 YY1012 pKa = 11.35 DD1013 pKa = 3.67 RR1014 pKa = 11.84 GPSRR1018 pKa = 11.84 LKK1020 pKa = 10.42 QVLATSIDD1028 pKa = 3.98 LNGFASVVNFASKK1041 pKa = 9.77 WLQSSDD1047 pKa = 3.43 SPNLYY1052 pKa = 8.81 RR1053 pKa = 11.84 TGEE1056 pKa = 4.08 PPP1058 pKa = 3.37
Molecular weight: 122.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.287
IPC2_protein 8.331
IPC_protein 8.17
Toseland 8.668
ProMoST 8.712
Dawson 9.092
Bjellqvist 8.99
Wikipedia 9.253
Rodwell 9.209
Grimsley 9.092
Solomon 9.121
Lehninger 9.077
Nozaki 9.033
DTASelect 8.887
Thurlkill 8.975
EMBOSS 9.209
Sillero 9.151
Patrickios 4.406
IPC_peptide 9.107
IPC2_peptide 7.644
IPC2.peptide.svr19 7.637
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1351
293
1058
675.5
78.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.625 ± 0.393
1.11 ± 0.04
5.996 ± 0.089
6.44 ± 1.27
5.922 ± 0.57
5.329 ± 0.877
1.925 ± 0.412
5.848 ± 0.292
8.142 ± 1.272
9.845 ± 0.18
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.813 ± 0.194
5.329 ± 0.216
3.183 ± 0.676
3.997 ± 0.67
5.922 ± 0.367
7.624 ± 0.209
4.071 ± 0.145
5.107 ± 0.463
1.258 ± 0.263
4.515 ± 1.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here