Trichormus variabilis SAG 1403-4b
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5168 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S5K2M9|A0A3S5K2M9_ANAVA DUF262 domain-containing protein OS=Trichormus variabilis SAG 1403-4b OX=447716 GN=DSM107003_49640 PE=4 SV=1
MM1 pKa = 7.51 SRR3 pKa = 11.84 PQVKK7 pKa = 8.45 ITVSASGGPLNTPSYY22 pKa = 11.08 KK23 pKa = 10.6 DD24 pKa = 3.35 LTITGIGTDD33 pKa = 3.27 NTYY36 pKa = 9.32 TFLNQQGVLDD46 pKa = 4.29 AWCLDD51 pKa = 3.38 SATGISSNTTYY62 pKa = 9.86 TANVYY67 pKa = 10.52 SSYY70 pKa = 11.25 EE71 pKa = 3.96 LGALATLGTPARR83 pKa = 11.84 IGNLYY88 pKa = 10.75 NLDD91 pKa = 3.66 LVNWIINQNFAANPQYY107 pKa = 11.31 NYY109 pKa = 11.33 GEE111 pKa = 4.0 IQAAIWTLLGDD122 pKa = 4.53 TYY124 pKa = 11.37 NSSGADD130 pKa = 3.34 LTRR133 pKa = 11.84 NGAVVASDD141 pKa = 3.64 VNSIIALAKK150 pKa = 10.31 ANGEE154 pKa = 4.04 NFVPDD159 pKa = 3.71 TNQFIGVIFDD169 pKa = 3.81 PFVVNGDD176 pKa = 3.55 GSVTPRR182 pKa = 11.84 QPLIAQIKK190 pKa = 8.18 AAKK193 pKa = 10.34 LGDD196 pKa = 4.28 LVWNDD201 pKa = 3.39 LNANGIQDD209 pKa = 3.65 TGEE212 pKa = 3.92 SGIAGVTVNLTRR224 pKa = 11.84 DD225 pKa = 3.52 SNSNGIIEE233 pKa = 4.47 SNEE236 pKa = 3.42 ILATTTTNVSGLYY249 pKa = 9.12 EE250 pKa = 4.26 FRR252 pKa = 11.84 GLTPGLSYY260 pKa = 10.35 QVQFATPTGFDD271 pKa = 3.13 AVSPRR276 pKa = 11.84 QQGGNQTTDD285 pKa = 2.4 SDD287 pKa = 4.27 GLVSNVVILNPGEE300 pKa = 3.98 FNQTIDD306 pKa = 3.17 AGFYY310 pKa = 10.2 KK311 pKa = 10.51 YY312 pKa = 11.03 ASLGDD317 pKa = 3.59 FVFNDD322 pKa = 3.68 GNNNGIQDD330 pKa = 3.76 AGEE333 pKa = 4.05 AGIGGVTVEE342 pKa = 5.58 LINPANGSVIATTTTDD358 pKa = 3.82 GIGAYY363 pKa = 8.44 TFTGLTPGDD372 pKa = 3.82 YY373 pKa = 9.53 QVKK376 pKa = 8.04 FTSPTGYY383 pKa = 10.33 VFSTANVGDD392 pKa = 3.85 DD393 pKa = 4.3 TKK395 pKa = 11.6 DD396 pKa = 3.11 SDD398 pKa = 4.67 ANTSTGLTKK407 pKa = 10.23 TVTLTSGEE415 pKa = 4.31 FNGTLDD421 pKa = 3.63 AGLVQLASLGDD432 pKa = 3.93 KK433 pKa = 10.5 VFHH436 pKa = 7.25 DD437 pKa = 5.01 LDD439 pKa = 5.64 ADD441 pKa = 5.05 GIQDD445 pKa = 3.86 AGEE448 pKa = 3.86 QGIANASVKK457 pKa = 10.77 LLDD460 pKa = 3.56 TAGNVIATTTTDD472 pKa = 3.08 GDD474 pKa = 3.85 GLYY477 pKa = 10.64 SFTNLQPGDD486 pKa = 3.73 YY487 pKa = 9.59 KK488 pKa = 11.37 VQFVQPTGFNGVSLANVGGDD508 pKa = 4.01 DD509 pKa = 4.78 SVDD512 pKa = 3.57 SDD514 pKa = 4.01 GLISDD519 pKa = 4.49 VVNLSPGEE527 pKa = 3.94 NDD529 pKa = 3.38 TTVDD533 pKa = 2.85 AGFYY537 pKa = 8.57 KK538 pKa = 10.21 TASLGDD544 pKa = 3.5 FVFNDD549 pKa = 3.68 GNNNGIQDD557 pKa = 3.76 AGEE560 pKa = 4.06 AGIGEE565 pKa = 4.41 VTVEE569 pKa = 5.02 LINPADD575 pKa = 3.81 GSVIATTTTDD585 pKa = 2.84 EE586 pKa = 4.13 TGAYY590 pKa = 7.47 TFTGLTPGDD599 pKa = 3.82 YY600 pKa = 9.53 QVKK603 pKa = 8.25 FTSPTDD609 pKa = 3.87 YY610 pKa = 11.14 VFSTADD616 pKa = 2.99 QGGDD620 pKa = 3.44 DD621 pKa = 4.5 TVDD624 pKa = 3.2 SDD626 pKa = 5.04 ANTSTGLTQTVTLTSGEE643 pKa = 4.31 FNGTLDD649 pKa = 3.63 AGLVQLASLGDD660 pKa = 3.84 FVFEE664 pKa = 4.86 DD665 pKa = 3.94 SNANGIQDD673 pKa = 3.09 AWEE676 pKa = 3.81 QGIAEE681 pKa = 4.27 ASVNLLDD688 pKa = 3.78 TAGNVIATTTTDD700 pKa = 3.08 GDD702 pKa = 3.85 GLYY705 pKa = 10.64 SFTNLQPGDD714 pKa = 3.73 YY715 pKa = 9.59 KK716 pKa = 11.41 VQFVQPAGFSGVSPSNVGGDD736 pKa = 3.97 DD737 pKa = 4.02 SVDD740 pKa = 3.57 SDD742 pKa = 4.01 GLISDD747 pKa = 4.49 VVNLSPGEE755 pKa = 3.94 NDD757 pKa = 3.38 TTVDD761 pKa = 2.85 AGFYY765 pKa = 8.57 KK766 pKa = 10.21 TASLGDD772 pKa = 3.5 FVFNDD777 pKa = 3.68 GNNNGIQDD785 pKa = 3.45 IGEE788 pKa = 4.16 AGIGGVTVEE797 pKa = 5.58 LINPANGSVIASVITDD813 pKa = 3.89 DD814 pKa = 3.48 NGGYY818 pKa = 9.52 QFSGLTPGDD827 pKa = 3.7 YY828 pKa = 9.61 QVKK831 pKa = 8.04 FTSPTGYY838 pKa = 10.34 VFSTAYY844 pKa = 10.15 QGGDD848 pKa = 3.36 DD849 pKa = 4.57 TVDD852 pKa = 3.2 SDD854 pKa = 5.04 ANTSTGLTKK863 pKa = 10.23 TVTLTSGEE871 pKa = 4.31 FNGTLDD877 pKa = 3.63 AGLVQLASLGDD888 pKa = 3.93 KK889 pKa = 10.5 VFHH892 pKa = 7.25 DD893 pKa = 5.01 LDD895 pKa = 5.64 ADD897 pKa = 5.05 GIQDD901 pKa = 3.86 AGEE904 pKa = 3.86 QGIANASVKK913 pKa = 10.77 LLDD916 pKa = 3.56 TAGNVIATTTTDD928 pKa = 3.08 GDD930 pKa = 3.88 GLYY933 pKa = 10.82 SFINLQPGDD942 pKa = 3.83 YY943 pKa = 9.82 KK944 pKa = 11.37 VQFVQPTDD952 pKa = 3.8 FNGVSLANVGGDD964 pKa = 4.01 DD965 pKa = 4.78 SVDD968 pKa = 3.57 SDD970 pKa = 4.01 GLISDD975 pKa = 4.49 VVNLSPGEE983 pKa = 3.94 NDD985 pKa = 3.38 TTVDD989 pKa = 2.85 AGFYY993 pKa = 8.57 KK994 pKa = 10.21 TASLGDD1000 pKa = 3.5 FVFNDD1005 pKa = 3.68 GNNNGIQDD1013 pKa = 3.76 AGEE1016 pKa = 4.05 AGIGGVTVKK1025 pKa = 10.8 LINPANGNVISTTTTDD1041 pKa = 3.03 GAGAYY1046 pKa = 7.07 TFTGLTPGDD1055 pKa = 3.84 YY1056 pKa = 9.35 QVKK1059 pKa = 9.7 FISPTGYY1066 pKa = 10.67 SFTAANQGGNDD1077 pKa = 4.28 AVDD1080 pKa = 3.56 SDD1082 pKa = 4.92 ANPSTGLTQTVTLTSGEE1099 pKa = 4.32 YY1100 pKa = 10.52 NGTLDD1105 pKa = 4.27 AGLVQLASLGDD1116 pKa = 3.93 KK1117 pKa = 10.5 VFHH1120 pKa = 7.25 DD1121 pKa = 5.01 LDD1123 pKa = 5.64 ADD1125 pKa = 5.05 GIQDD1129 pKa = 3.86 AGEE1132 pKa = 3.86 QGIANASVKK1141 pKa = 10.77 LLDD1144 pKa = 3.56 TAGNVIATTTTDD1156 pKa = 3.08 GDD1158 pKa = 3.85 GLYY1161 pKa = 10.64 SFTNLQPGDD1170 pKa = 3.73 YY1171 pKa = 9.59 KK1172 pKa = 11.37 VQFVQPTGFNGVSLANVGGDD1192 pKa = 3.78 DD1193 pKa = 5.29 SVDD1196 pKa = 3.18 SDD1198 pKa = 3.89 AASNLTTGVVNLSPGEE1214 pKa = 3.94 NDD1216 pKa = 3.38 TTVDD1220 pKa = 2.85 AGFYY1224 pKa = 8.64 KK1225 pKa = 10.25 TASLGNFVFNDD1236 pKa = 3.34 VNNNGIQEE1244 pKa = 3.98 ASEE1247 pKa = 4.27 AGVAGVTVTLTGGGADD1263 pKa = 3.84 GLISTTADD1271 pKa = 2.81 NTTITTTTNASGKK1284 pKa = 10.27 YY1285 pKa = 9.23 NFAGLTPGQEE1295 pKa = 4.26 YY1296 pKa = 10.07 QVGFSNLPAGYY1307 pKa = 10.36 QFTQANAGTDD1317 pKa = 3.53 DD1318 pKa = 5.35 ALDD1321 pKa = 3.86 SDD1323 pKa = 5.42 ANTSTGKK1330 pKa = 7.77 TQIVTLASGEE1340 pKa = 4.29 NNTTLDD1346 pKa = 3.17 AGIYY1350 pKa = 9.65 QNAGDD1355 pKa = 4.69 LSITKK1360 pKa = 9.53 TNGLTTVSPGQQITYY1375 pKa = 9.2 TIVAQNNGLITATNALVSDD1394 pKa = 5.22 IIPSNLTNVTWTSVATGGATDD1415 pKa = 3.9 NQSSGTGSINDD1426 pKa = 4.01 YY1427 pKa = 9.5 VTLTAGSSITYY1438 pKa = 8.23 TVTGTVAPNALTPVYY1453 pKa = 8.18 NTPTTINLTSNSSSALNGTAGNIRR1477 pKa = 11.84 NFSANGISVNASAFSSTSTGTWNTAYY1503 pKa = 10.68 LGLYY1507 pKa = 9.38 GSYY1510 pKa = 11.06 GLGVTDD1516 pKa = 4.27 GGEE1519 pKa = 3.89 NGSNGTHH1526 pKa = 7.02 HH1527 pKa = 7.36 IDD1529 pKa = 3.12 NDD1531 pKa = 3.32 GRR1533 pKa = 11.84 NNYY1536 pKa = 9.11 VLLEE1540 pKa = 4.14 FSDD1543 pKa = 3.85 SVVIDD1548 pKa = 3.95 KK1549 pKa = 10.9 AALSYY1554 pKa = 11.27 VVGDD1558 pKa = 3.5 SDD1560 pKa = 3.62 ISVWIGNFNNPYY1572 pKa = 9.47 TNHH1575 pKa = 6.17 LTLNSSVLSSFGFTEE1590 pKa = 3.95 INLGGSSDD1598 pKa = 3.79 RR1599 pKa = 11.84 NADD1602 pKa = 3.12 INASNIVGNAVVIAAKK1618 pKa = 10.73 VGDD1621 pKa = 3.98 TNDD1624 pKa = 3.43 EE1625 pKa = 4.37 FKK1627 pKa = 11.13 LKK1629 pKa = 10.81 SLDD1632 pKa = 3.5 IIKK1635 pKa = 9.23 ATTQTGGSLVNTATITAPTGFTDD1658 pKa = 3.58 TNSNNNSATDD1668 pKa = 3.23 TDD1670 pKa = 4.38 TIVASSTVRR1679 pKa = 11.84 IGDD1682 pKa = 3.74 RR1683 pKa = 11.84 VWYY1686 pKa = 7.32 DD1687 pKa = 3.32 TNANGIQEE1695 pKa = 4.16 TGEE1698 pKa = 4.0 AGVAGVAVNLFNQAGTQVGTTTTDD1722 pKa = 2.83 TNGFYY1727 pKa = 10.54 QFTANTNSTYY1737 pKa = 10.71 SVQFVQPTGFSGFTTANVGNDD1758 pKa = 3.61 GADD1761 pKa = 3.24 SDD1763 pKa = 4.55 VVNANGTTAQFLVGTTDD1780 pKa = 3.26 NLTIDD1785 pKa = 3.64 AGLIKK1790 pKa = 10.76 NIGDD1794 pKa = 3.81 LSITKK1799 pKa = 9.48 TDD1801 pKa = 3.16 GLTTVTAGQQLTYY1814 pKa = 10.31 TIVVSNNGFITATNALVTDD1833 pKa = 5.92 AIPALLTNVTWTSVASGGATGNEE1856 pKa = 3.93 TSGTGNINDD1865 pKa = 3.9 YY1866 pKa = 9.57 VTLTGGSSITYY1877 pKa = 7.49 TVNGTVAANAVSSSSTYY1894 pKa = 9.84 TDD1896 pKa = 2.52 TRR1898 pKa = 11.84 FDD1900 pKa = 4.14 FNGNSSSDD1908 pKa = 3.44 GTDD1911 pKa = 2.82 GNTRR1915 pKa = 11.84 TFTKK1919 pKa = 10.61 DD1920 pKa = 2.88 GITVTARR1927 pKa = 11.84 AFSRR1931 pKa = 11.84 VDD1933 pKa = 3.26 GTNGAWSKK1941 pKa = 11.29 AYY1943 pKa = 10.28 LGSYY1947 pKa = 9.13 TGGLGVTDD1955 pKa = 4.03 SSEE1958 pKa = 3.83 SSSYY1962 pKa = 10.79 HH1963 pKa = 6.01 RR1964 pKa = 11.84 VDD1966 pKa = 3.43 NGGGRR1971 pKa = 11.84 DD1972 pKa = 3.58 NYY1974 pKa = 10.9 VLFQFSEE1981 pKa = 4.37 AVVVDD1986 pKa = 3.89 SAYY1989 pKa = 10.66 LQYY1992 pKa = 11.4 VVTDD1996 pKa = 3.36 SDD1998 pKa = 3.82 TSVWIGNFNNTITTLSDD2015 pKa = 3.22 SVLNSFGFTEE2025 pKa = 4.4 VNLGSGSDD2033 pKa = 3.11 RR2034 pKa = 11.84 WADD2037 pKa = 3.27 INAGNYY2043 pKa = 9.46 LGNTLVIAAKK2053 pKa = 9.9 TDD2055 pKa = 3.43 DD2056 pKa = 3.81 TNDD2059 pKa = 3.23 NFKK2062 pKa = 10.32 IRR2064 pKa = 11.84 HH2065 pKa = 6.24 LDD2067 pKa = 3.04 IQKK2070 pKa = 9.94 PVAPTTVSLTNTATVTAPTGFTDD2093 pKa = 3.71 TNTSNNSATDD2103 pKa = 3.44 TDD2105 pKa = 4.48 TVTAAPTVPSAPGVRR2120 pKa = 11.84 TPGFWQNTEE2129 pKa = 3.64 WQKK2132 pKa = 10.89 FWDD2135 pKa = 5.33 GIQGNEE2141 pKa = 4.28 PAQKK2145 pKa = 8.27 TQPNFAKK2152 pKa = 10.33 SDD2154 pKa = 3.87 LLFAPYY2160 pKa = 9.58 TNSAQAGKK2168 pKa = 10.59 VLDD2171 pKa = 4.33 PVSGQYY2177 pKa = 10.67 DD2178 pKa = 3.32 IGLLIGDD2185 pKa = 4.45 FNRR2188 pKa = 11.84 NGKK2191 pKa = 7.69 TDD2193 pKa = 3.35 AGEE2196 pKa = 4.01 DD2197 pKa = 3.46 TLFYY2201 pKa = 10.77 TRR2203 pKa = 11.84 AQALQMVDD2211 pKa = 4.39 ASAHH2215 pKa = 5.63 PNGDD2219 pKa = 2.69 KK2220 pKa = 10.61 RR2221 pKa = 11.84 YY2222 pKa = 9.23 DD2223 pKa = 3.4 LGRR2226 pKa = 11.84 SLVAGWLNYY2235 pKa = 10.25 LAGNPIDD2242 pKa = 3.98 TANPTDD2248 pKa = 3.68 KK2249 pKa = 11.14 DD2250 pKa = 3.02 EE2251 pKa = 4.64 RR2252 pKa = 11.84 YY2253 pKa = 10.42 YY2254 pKa = 10.51 INEE2257 pKa = 4.76 GINWLQGFTPDD2268 pKa = 4.17 EE2269 pKa = 4.51 NGDD2272 pKa = 3.75 KK2273 pKa = 10.95 KK2274 pKa = 11.25 GDD2276 pKa = 3.45 GAIYY2280 pKa = 10.45 QMTGSSVSSPQLTNSYY2296 pKa = 9.01 WNLGISSASNLPSSYY2311 pKa = 10.82 KK2312 pKa = 10.79 SNTNVLYY2319 pKa = 9.51 PLEE2322 pKa = 4.67 SGSVINTNLDD2332 pKa = 3.28 NYY2334 pKa = 11.31 NNGLGLADD2342 pKa = 3.36 NVFYY2346 pKa = 11.01 GGNAA2350 pKa = 3.15
Molecular weight: 242.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.605
IPC_protein 3.681
Toseland 3.427
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.503
Grimsley 3.338
Solomon 3.694
Lehninger 3.656
Nozaki 3.795
DTASelect 4.126
Thurlkill 3.49
EMBOSS 3.668
Sillero 3.808
Patrickios 1.697
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.742
Protein with the highest isoelectric point:
>tr|A0A433UWE5|A0A433UWE5_ANAVA Uncharacterized protein OS=Trichormus variabilis SAG 1403-4b OX=447716 GN=DSM107003_12610 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.03 PNYY5 pKa = 9.07 RR6 pKa = 11.84 RR7 pKa = 11.84 CISCRR12 pKa = 11.84 RR13 pKa = 11.84 VGLKK17 pKa = 10.37 EE18 pKa = 3.9 EE19 pKa = 4.36 FWRR22 pKa = 11.84 IVRR25 pKa = 11.84 VFPSGKK31 pKa = 9.1 VQLDD35 pKa = 3.24 QGMGRR40 pKa = 11.84 SAYY43 pKa = 9.32 ICPQTSCLQAAQKK56 pKa = 10.5 KK57 pKa = 8.83 NRR59 pKa = 11.84 LGRR62 pKa = 11.84 SLHH65 pKa = 5.08 GTVPEE70 pKa = 4.21 TLYY73 pKa = 9.23 QTLWLRR79 pKa = 11.84 LTQNNTQNQII89 pKa = 3.23
Molecular weight: 10.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.706
IPC_protein 10.452
Toseland 10.599
ProMoST 10.292
Dawson 10.716
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.965
Grimsley 10.774
Solomon 10.804
Lehninger 10.774
Nozaki 10.628
DTASelect 10.423
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.657
Patrickios 10.716
IPC_peptide 10.818
IPC2_peptide 9.721
IPC2.peptide.svr19 8.447
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5168
0
5168
1610029
41
3312
311.5
34.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.704 ± 0.033
0.971 ± 0.012
4.838 ± 0.027
6.374 ± 0.034
3.972 ± 0.023
6.499 ± 0.039
1.805 ± 0.016
7.239 ± 0.03
5.234 ± 0.034
10.926 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.834 ± 0.016
4.772 ± 0.036
4.565 ± 0.027
5.388 ± 0.034
4.73 ± 0.028
6.382 ± 0.03
5.753 ± 0.029
6.487 ± 0.028
1.408 ± 0.015
3.109 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here